DEVELOPMENT OF AN INTEGRATED MANAGEMENT APPROACHES FOR MUNGBEAN YELLOW MOSAIC VIRUS OF GREEN GRAM [VIGNA RADIATA (L.) WILCZEK ] IN BUNDELKHAND ZONE

2018 ◽  
Vol 24 (1) ◽  
Author(s):  
ANIL KUMAR SINGH ◽  
J.K. BABELE ◽  
R.K.S. TOMAR
Keyword(s):  
Mosaic Virus ◽  
Seed Treatment ◽  
Integrated Management ◽  
Green Gram ◽  
Yellow Mosaic Virus ◽  
Economic Losses ◽  
Resistant Variety ◽  
Cost Ratio ◽  
Benefit Cost Ratio ◽  
Mungbean Yellow Mosaic Virus

Mungbean yellow mosaic virus (MYMV) disease is one of the most vicious diseases of green gram and has been renowned in India for more than five decades. The economic losses due to this virus account up to 85% in green gram which is spreading faster towards newer areas. A field experiment was carried out by Krishi Vigyan Kendra during kharif seasons at the farmers’ fields in villages Sitapur, Datia district of BundelKhand zone to evaluate the five integrated pest management module including conventional farmers’ practices against green gram sucking pest (white fly) and YMV. Among the test modules, Module 3 (Resistant variety TJM 3+ Seed treatment with Imidacloprid 17.8 % SL @ 5 ml/kg seeds +2 sprays of Imidacloprid 17.8 % SL @ 0.5 ml/l water) found as the most effective treatments with more than 91.58 percent mean reduction in nymphal population of whiteflies and below 2 percent incidence of YMV followed by Module 2 (Module 1 +2 sprays of Neem oil (5% Azadirachtin) @ 3.0 ml/l), Module 4 (Module 1 + 2 sprays of Triazophos 40 % EC @ 1.5 ml/l), Module 1(Resistant variety TJM 3+ Seed treatment with Imidacloprid 17.8 % SL @ 5 ml/kg seeds). All the IPM modules were found significantly superior over the conventional farmers’ practices in reducing the incidence of YMV in black gram. Higher mean yield of 9.35 q/ha in Module 3 followed by Module 2, Module 4, and Module 1 were recorded in the IPM fields against 6.75 q/ha in farmers’ practise fields. Module 3 obtained maximum return with higher benefit cost ratio followed by Module 2, Module 4, and Module 1.

scholarly journals Screening for Mungbean Yellow Mosaic Virus Resistance in Green Gram (Vigna radiata (L.) Wilczek)

2019 ◽  
Vol 8 (10) ◽  
pp. 666-670
Author(s):  
K. R. Reshmi Raj ◽  
B. Baisakh ◽  
S. K. Tripathy ◽  
Devraj Lenka ◽  
B. Pradhan ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Resistance ◽  
Vigna Radiata ◽  
Green Gram ◽  
Yellow Mosaic Virus ◽  
Mungbean Yellow Mosaic Virus

Mungbean yellow mosaic virus (MYMV): a threat to green gram (Vigna radiata) production in Asia

2014 ◽  
Vol 60 (4) ◽  
pp. 314-324 ◽  
Author(s):  
A. Karthikeyan ◽  
V.G. Shobhana ◽  
M. Sudha ◽  
M. Raveendran ◽  
N. Senthil ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Vigna Radiata ◽  
Green Gram ◽  
Yellow Mosaic Virus ◽  
Mungbean Yellow Mosaic Virus

scholarly journals Molecular Cloning of MYMV Genome and Infectivity of Yellow Mosaic Virus in Green Gram Using Different Viral Transmission Tools

2021 ◽  
Vol 18 (3) ◽  
pp. 467-478
Author(s):  
Ashwini Talakayala ◽  
Veerapaneni Bindu Prathyusha ◽  
Dhanasekar Divya ◽  
Srinivas Ankanagari ◽  
Mallikarjuna Garladinne
Keyword(s):  
Mosaic Virus ◽  
Infectious Clone ◽  
Viral Transmission ◽  
Green Gram ◽  
Yellow Mosaic Virus ◽  
Rolling Circle ◽  
Plant Expression Vector ◽  
Bipartite Genome ◽  
Mungbean Yellow Mosaic Virus ◽  
Polymerase Chain

Mungbean yellow mosaic virus (MYMV) causes massive crop losses in green gram. MYMV is a member of begomovirus with bipartite genome comprising DNA-A and DNA-B components, which is transmitted by whiteflies. Cloning and preparation of infectious clone is very much essential for screening germplasm or transgenic material of pulse crops since viruliferous whiteflies may not be available throughout the year. In the current work, we have amplified rolling circle mediated viral genome of MYMV using Φ29 DNA polymerase. The amplified products was digested and cloned into the plant expression vector pCAMBIA2301.The cloned constructs was then transformed into Agrobacterium LBA4404 through freeze thaw method. Further, three viral transmission techniques including mechanical rubbing, Agroinfiltration and Agroinoculation, were employed for assessing the mosaic symptoms in green gram. The molecular confirmation through polymerase chain reaction (PCR) indicated that the yellow mosaic symptoms were formed due to infectivity of MYMV in the green gram.

Comparative Effectiveness Of Seed Treating And Foliar Insecticides Against Sucking Pests Of Cotton And Impact On Their Natural Enemies

2013 ◽  
Vol 38 (1) ◽  
pp. 61-70 ◽  
Author(s):  
SMA Hossain ◽  
MA Baque ◽  
MR Amin
Keyword(s):  
Natural Enemies ◽  
Untreated Control ◽  
Seed Treatment ◽  
Foliar Spray ◽  
Cost Ratio ◽  
Research Station ◽  
Benefit Cost Ratio ◽  
Benefit Cost ◽  
Thrips Population ◽  
Cotton Cultivar

The Imidacloprid insecticide, Gaucho 70 WS at 1.5, 2.5, 3.5, 4.5 and 5.5 g/kg seed was used as seed treatment and monocrotophos 40 WSC at 1120 ml/ha was applied as foliar spray on CB9 cotton cultivar to suppress aphid, whitefly and thrips, and impact on their natural enemies during 2008-2011 at the Regional Cotton Research Station, Dinajpur, Bangladesh. The activity of natural enemies, such as ladybird beetle, lacewing, syrphid, and spider population on the sucking pests attacking cotton cultivar CB9 and yield of cotton were recorded. Imidacloprid significantly reduced aphid, whitefly, and thrips population on cotton crops compared to untreated control or foliar spray of monocrotophos 40 WSC at 1120 ml/ha. Ladybird beetles, lacewings, syrphids, and spiders were abundant in the field but their population decreased in the treated plots compared to untreated control. The CB9 cotton cultivar produced significantly higher yield (1.73 t/ha) with a benefit cost ratio 12.47 when seeds were treated with Imidacloprid at 5.5 g/kg fuzzy seed. This study indicated that Imidacloprid (Gaucho 70 WS) used as a seed treatment may be suggested to the cotton growers for controlling sucking pests. Bangladesh J. Agril. Res. 38(1): 61-70, March 2013 DOI: http://dx.doi.org/10.3329/bjar.v38i1.15190

scholarly journals Breeding for Enhancing Legumovirus Resistance in Mungbean: Current Understanding and Future Directions

Agronomy ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 622 ◽  
Author(s):  
Chandra Mohan Singh ◽  
Poornima Singh ◽  
Aditya Pratap ◽  
Rakesh Pandey ◽  
Shalini Purwar ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Seed Quality ◽  
Soil Health ◽  
Mosaic Disease ◽  
Bipartite Begomoviruses ◽  
Yellow Mosaic Virus ◽  
Symbiotic Association ◽  
Detection Techniques ◽  
Vigna Species ◽  
Mungbean Yellow Mosaic Virus

Yellow mosaic disease (YMD) affects several types of leguminous crops, including the Vigna species, which comprises a number of commercially important pulse crops. YMD is characterized by the formation of a bright yellow mosaic pattern on the leaves; in severe forms, this pattern can also be seen on stems and pods. This disease leads to tremendous yield losses, even up to 100%, in addition to deterioration in seed quality. Symptoms of this disease are similar among affected plants; YMD is not limited to mungbean (Vigna radiata L. Wilczek) and also affects other collateral and alternate hosts. In the last decade, rapid advancements in molecular detection techniques have been made, leading to an improved understanding of YMD-causing viruses. Three distinct bipartite begomoviruses, namely, Mungbean Yellow Mosaic India Virus (MYMIV), Mungbean Yellow Mosaic Virus (MYMV), and Horsegram Yellow Mosaic Virus (HgYMV), are known to cause YMD in Vigna spp. Vigna crops serve as an excellent protein source for vegetarians worldwide; moreover, they aid in improving soil health by fixing atmospheric nitrogen through a symbiotic association with Rhizobium bacteria. The loss in the yield of these short-duration crops due to YMD, thus, needs to be checked. This review highlights the discoveries that have been made regarding various aspects of YMD affecting mungbean, including the determination of YMD-causing viruses and strategies used to develop high-yielding YMD-resistant mungbean varieties that harness the potential of related Vigna species through the use of different omics approaches.

​RCA-based Detection of Begomoviruses in Weed Ggenera Associated with Legumes in Southern Karnataka

2021 ◽  
Author(s):  
Sudeep Pandey ◽  
T.R. Girish ◽  
S. Basavaraj ◽  
A.S. Padmaja ◽  
N. Nagaraju
Keyword(s):  
Mosaic Virus ◽  
Rolling Circle Amplification ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Insect Vector ◽  
Weed Species ◽  
Ageratum Conyzoides ◽  
Yellow Mosaic Disease ◽  
Mungbean Yellow Mosaic Virus ◽  
Legume Crops

Background: Yellow mosaic disease (YMD) caused by begomoviruses transmitted through the insect vector Bemisia tabaci poses a serious threat to the production of legume crops. Methods: Season-long surveys were carried out for YMD occurrence in six different legume crops and associated natural weeds both symptomatic and asymptomatic across the districts of southern Karnataka, India. The samples were analyzed through RCA PCR using specific primer pairs. Result: Up to 94.1 per cent YMD incidence was recorded and nine weed species were commonly found associated with legume crops. The weeds viz., Ageratum conyzoides, Alternanthera sessilis, Commelina benghalensis and Euphorbia geniculata were abundantly found in the surveyed regions. The weeds were both symptomatic and asymptomatic. Rolling circle amplification coupled polymerase chain reaction method was employed to detect yellow mosaic virus in asymptomatic weeds. Phylogenetic analysis based on the sequences of PCR amplified products of weeds and symptomatic legumes revealed a close clustering of the weed samples with horsegram yellow mosaic virus, legume yellow mosaic virus and mungbean yellow mosaic virus. Overall, our data suggests the role of weed species associated with legume crops as alternative/collateral hosts of begomoviruses and their role in the epidemiology of yellow mosaic disease.

Virus Vector Relationship of Yellow Mosaic Virus and Whitefly, Bemisia tabaci (Gennadius) in Soybean

2021 ◽  
Author(s):  
M. Swathi ◽  
Neeta Gaur ◽  
Kamendra Singh
Keyword(s):  
Mosaic Virus ◽  
Virus Disease ◽  
Management Strategies ◽  
Virus Transmission ◽  
Virus Vector ◽  
Yellow Mosaic Virus ◽  
Starvation Period ◽  
Mungbean Yellow Mosaic Virus ◽  
Biological Relationship ◽  
Relationship Of

Background: Whitefly is one of the most destructive sucking pest in the tropical and subtropical regions of the world and causing significant crop losses directly by sucking sap from the plants and indirectly through the transmission of viral diseases specifically caused by the genus Begomovirus. The Begomovirus species viz., Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV) are causing yellow mosaic virus disease in soybean, which is transmitted by whiteflies. The disease accounts to 30-70 per cent yield loss and increases up to 80 - 100 per cent during severe incidence. Hence, there is a need for development of integrated pest management strategies against disease and whiteflies, for this the knowledge on virus-vector relationship is required. But, the studies on biological relationship of yellow mosaic virus disease and whitefly in soybean are scarce. At this juncture, considering the importance of disease in soybean, the present investigation was carried out to know the virus -vector relationship of the YMV and whitefly in soybean.Methods: The experiment on virus-vector relationship of yellow mosaic virus and whitefly in soybean was conducted at Department of Entomology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand during 2016-17. The data on number of whiteflies per plant, acquisition and inoculation access feeding period and pre and post starvation period required for effective transmission of virus was recorded.Result: A single viruliferous whitefly was able to transmit virus and ten viruliferous whiteflies per plant were required for cent per cent transmission of virus. The minimum acquisition access and inoculation access feeding periods required for virus transmission was 0.25h (15 min) each; while the 100 per cent virus transmission was recorded with acquisition and inoculation period of 12h, each. The per cent transmission was increased with the increase of acquisition and inoculation periods. The rate of transmission was positively correlated with pre-acquisition starvation period and negatively correlated with post- acquisition starvation period.

Sign in / Sign up

Export Citation Format

Share Document