scholarly journals Association of Early Pubertal Onset in Female Rats With Inhalation of Lavender Oil

2022 ◽  
Vol 37 (2) ◽  
Author(s):  
Yoo-Mi Kim ◽  
Han Hyuk Lim
Keyword(s):  
Female Rats ◽  
Lavender Oil ◽  
Pubertal Onset

scholarly journals Association of Early Pubertal Onset in Female Rats with Inhalation of Lavender Oil

2021 ◽  
Author(s):  
Yoo-Mi Kim ◽  
Han H. Lim
Keyword(s):  
Precocious Puberty ◽  
Endocrine Disrupting Chemicals ◽  
Central Precocious Puberty ◽  
Female Rats ◽  
Vaginal Opening ◽  
Lavender Oil ◽  
Hormone Levels ◽  
Pubertal Onset ◽  
Endocrine Disrupting ◽  
Body Weights

Abstract Background Central precocious puberty is caused by early activation of the hypothalamic–pituitary–gonadal axis but its major cause remains unclear. Studies have indicated an association between chronic environmental exposure to endocrine-disrupting chemicals and pubertal onset. Methods To evaluate this association, we compared the hormone levels and timing of vaginal opening in female rats exposed to lavender oil (LO) through different routes [study groups: control, LO nasal spray (LS), and indoor exposure to LO (LE)] during the prepubertal period. The body weights of the animals were also compared every 3 days until the day of vaginal opening, at which time gonadotropin levels and internal organ weights were assessed. Results The LS group showed early vaginal opening at 33.8 ± 1.8 days compared with the control (38.4 ± 2.9 days) and LE (36.6 ± 1.5 days) groups. Additionally, luteinizing hormone levels were significantly higher in the LE and LS groups than in the control group. Body weights did not differ significantly among the groups. Conclusions Exposure to an exogenic simulant via inhalation during the prepubertal period triggered early puberty onset in female rats. Further evaluation of exposure to other endocrine-disrupting chemicals capable of inducing central precocious puberty through the skin, orally, and/or nasally is warranted.

Prepubertal exposure to T-2 toxin advances pubertal onset and development in female rats via promoting the onset of hypothalamic–pituitary–gonadal axis function

2016 ◽  
Vol 35 (12) ◽  
pp. 1276-1285 ◽  
Author(s):  
R Yang ◽  
Y-M Wang ◽  
L Zhang ◽  
Z-M Zhao ◽  
J Zhao ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Female Rats ◽  
Intragastric Administration ◽  
Corpora Lutea ◽  
Sprague Dawley ◽  
Trichothecene Mycotoxin ◽  
Pubertal Onset ◽  
Serum Hormone ◽  
Puberty Onset ◽  
High Level

T-2 toxin, a naturally produced Type A trichothecene mycotoxin, has been shown to damage the reproductive and developmental functions in livestocks. However, whether T-2 toxin can disturb the pubertal onset and development following prepubertal exposure remains unclear. To clarify this point, infantile female Sprague–Dawley rats were given a daily intragastric administration of vehicle or T-2 toxin at a dose of 375 μg/kg body weight for 5 consecutive days from postnatal day (PND) 15–19 (PND15–PND19). The days of vaginal opening, first diestrus, and first estrus in regular estrous cycle were advanced following T-2 toxin treatment, indicating prepubertal exposure to T-2 toxin induced the advancement of puberty onset. The relative weights of uterus and ovaries and the incidence of corpora lutea were all increased in T-2 toxin-treated rats; serum hormone levels of luteinizing hormone and estradiol and the messenger RNA expressions of gonadotropin-releasing hormone (GnRH) and GnRH receptor also displayed marked increases following exposure to T-2 toxin, all of which were well consistent with the manifestations of the advanced puberty onset. In conclusion, the present study reveals that prepubertal exposure to a high level of T-2 toxin promotes puberty onset in infantile female rats by advancing the initiation of hypothalamic–pituitary–gonadal axis function in female rats.

scholarly journals Unaltered Development of the Initial Follicular Waves and Normal Pubertal Onset in Female Rats after Neonatal Deletion of the Follicular Reserve

Endocrinology ◽  
2003 ◽  
Vol 144 (8) ◽  
pp. 3651-3662 ◽  
Author(s):  
Céline J. Guigon ◽  
Séverine Mazaud ◽  
Maguelone G. Forest ◽  
Sylvie Brailly-Tabard ◽  
Noëlline Coudouel ◽  
...  
Keyword(s):  
Female Rats ◽  
Pubertal Onset ◽  
Follicular Waves ◽  
Follicular Reserve

scholarly journals Uterotrophic Assay of Percutaneous Lavender Oil in Immature Female Rats

2013 ◽  
Vol 32 (2) ◽  
pp. 123-129 ◽  
Author(s):  
Valerie T. Politano ◽  
Danielle McGinty ◽  
Elise M. Lewis ◽  
Alan M. Hoberman ◽  
Mildred S. Christian ◽  
...  
Keyword(s):  
Body Weight ◽  
Vehicle Control ◽  
Female Rats ◽  
Immature Female ◽  
Skin Reactions ◽  
Lavender Oil ◽  
The Third ◽  
Uterotrophic Assay ◽  
Body Weights ◽  
Weight Gains

The estrogenic potential of lavender oil was evaluated in a percutaneous uterotrophic bioassay in immature female rats. Four groups of 10 immature female rats each were randomly selected on postpartum day (PPD) 16. During the 3-day treatment period (PPDs 19-21), the immature rats were separated from the dams, caged in groups of 5 in a litter box for 6 hours, and administered the vehicle control article (corn oil) or lavender oil at 20 or 100 mg/kg per day. All dosages were administered as a 5 mL/kg volume in a Hilltop Chamber (25 mm diameter; absorbent material removed) placed on the shaved back of each immature rat, and secured with micropore tape and Vetrap. A positive control group was gavaged twice daily with 2.5 μg/kg per day of 17α-ethinyl estradiol. Daily observations included viability, clinical signs, body weights, and body weight gains. All rats were euthanized 24 hours after the third and final treatment, the uteri and ovaries were removed, and the paired ovaries and wet and blotted uterine weights were recorded. No unscheduled deaths occurred. No skin reactions were observed. Both dosages of lavender oil significantly reduced body weight gains after the third day of treatment, but terminal body weights and mean absolute and relative uterine weights did not differ significantly from vehicle control values. Positive controls showed significant increases in body weight and increased mean absolute and relative uterine weights as expected. Based on these data, lavender oil, at dosages of 20 or 100 mg/kg, was not active in the rat uterotrophic assay and gave no evidence of estrogenic activity.

Investigation of Irisin's Role in Pubertal Onset Physiology in Female Rats

2021 ◽  
Author(s):  
Esra Kutlu ◽  
İlker Tolga Özgen ◽  
Huri Bulut ◽  
Abdurrahim Koçyiğit ◽  
Savaş Üstünova ◽  
...  
Keyword(s):  
Female Rats ◽  
Pubertal Onset

scholarly journals Sex Differences in Pubertal Circadian and Ultradian Rhythmic Development Under Naturalistic Conditions

2021 ◽  
Author(s):  
Azure Dominique Grant ◽  
Linda Wilbrecht ◽  
Lance J Kriegsfeld
Keyword(s):  
Sex Differences ◽  
Temporal Structure ◽  
Pubertal Development ◽  
Early Adulthood ◽  
Female Rats ◽  
Male Rats ◽  
Vaginal Opening ◽  
Field Station ◽  
Pubertal Onset ◽  
Laboratory Conditions

Biological rhythms in core body temperature (CBT) provide informative markers of adolescent development under controlled laboratory conditions. However, it is unknown if these markers are preserved under more variable naturalistic conditions, and if CBT may therefore prove useful in a real-world setting. To evaluate this possibility, we examined fecal steroid concentrations and CBT rhythms from pre-adolescence (p26) through early adulthood (p76) in intact male and female rats under natural light and climate at the University of California, Berkeley Field Station. Despite greater environmental variability, CBT markers of pubertal onset and its rhythmic progression were comparable to those previously reported in laboratory conditions in female rats and extend actigraphy-based findings in males. Specifically, sex differences emerged in circadian rhythm (CR) power and temperature amplitude prior to pubertal onset and persisted into early adulthood, with females exhibiting elevated CBT and decreased CR power compared to males. Within-day (ultradian rhythm; UR) patterns also exhibited a pronounced sex difference associated with estrous cyclicity. Pubertal onset, defined by vaginal opening, preputial separation, and sex steroid concentrations, occurred later than previously reported under lab conditions for both sexes. Vaginal opening and increased fecal estradiol concentrations were closely tied to the commencement of 4-day oscillations in CBT and UR power in female rats. By contrast, preputial separation and the first rise in testosterone concentration were not associated with adolescent changes to CBT rhythms in male rats. Together, males and females exhibited unique temporal patterning of CBT and sex steroids across pubertal development, with tractable associations between hormonal concentrations, external development, and temporal structure in females. The preservation of these features outside the laboratory supports CBT as a strong candidate for translational pubertal monitoring under naturalistic conditions in females.

scholarly journals Impact of pubertal onset on region-specific Esr2 expression

2021 ◽  
Author(s):  
Carly M. Drzewiecki ◽  
Elli P. Sellinger ◽  
Janice M. Juraska
Keyword(s):  
Estrogen Receptor ◽  
Motor Cortex ◽  
Estrogen Receptor Alpha ◽  
Estrogen Receptor Beta ◽  
Gonadal Hormones ◽  
Specific Pattern ◽  
Female Rats ◽  
Female Rat ◽  
Early Postnatal Development ◽  
Pubertal Onset

AbstractIn female rats, pubertal onset is associated with maturation of the medial prefrontal cortex (mPFC) and mPFC-mediated behaviors. These behavioral and anatomical changes are likely due to effects of estrogen at the nuclear estrogen receptor beta (ERβ), which is expressed at higher levels than the estrogen receptor alpha (ERα) isoform in the adult mPFC. Researchers have previously quantified ERβ protein and Esr2 RNA in rodents during early postnatal development and adulthood, but an adolescent-specific trajectory of this receptor in the mPFC has not been documented. Given that levels of Esr2 can fluctuate in the presence or absence of estrogens, puberty and the subsequent rise in gonadal hormones could influence ERβ expression in the adolescent brain. To further explore this, we used RNAscope to quantify the amount of Esr2 mRNA in pre-pubertal adolescent, recently post-pubertal adolescent, and adult female rats. We show here that Esr2 expression decreases significantly in the mPFC, striatum and motor cortex between pre-pubertal adolescence and adulthood. In the mPFC, this decrease occurs rapidly at pubertal onset, with no significant decrease in Esr2 levels between the recently post-pubertal and adult cohort. In contrast in the striatum and motor cortex, there were no significant differences in the amount of Esr2 between pre- and post-pubertal females. Insofar as the amount of Esr2 is proportional to functional ERβ, these results suggest ERβ decreases in a region-specific pattern in response to pubertal onset and highlight a role for this receptor in the maturational events that occur in the female rat mPFC at puberty.

scholarly journals Influences of age and pubertal status on number of perineuronal nets in the rat medial prefrontal cortex

2020 ◽  
Author(s):  
Carly M. Drzewiecki ◽  
Jari Willing ◽  
Janice M. Juraska
Keyword(s):  
Prefrontal Cortex ◽  
White Matter ◽  
Female Rats ◽  
Perineuronal Net ◽  
Abrupt Decrease ◽  
Pubertal Onset ◽  
Inhibitory Function ◽  
Sensory Cortices ◽  
Medial Pfc

AbstractThe prefrontal cortex (PFC) is a late developing region of the cortex, and its protracted maturation during adolescence may confer a period of plasticity. Closure of critical, or sensitive, periods in sensory cortices coincides with perineuronal net (PNN) expression, leading to enhanced inhibitory function and synaptic stabilization. PNN density is known to increase across adolescence in the male rodent medial PFC (mPFC). However, the trajectory of female PNN development has not been explored nor has the potential role of pubertal onset in PNN expression. Here, we examined rats at four time points spanning adolescent development to quantify the number of PNNs in the mPFC, as well as the total volume of the prefrontal white matter. Additionally, because puberty coincides with broad behavioral and neuroanatomical changes, we collected tissue from age-matched pre- and post- pubertal siblings within a litter. Results indicate that both males and females show an increase the total number of mPFC PNNs and in white matter under the mPFC between postnatal day (P) 30 and P60. Male puberty did not affect PNNs, while female pubertal onset led to an abrupt decrease in the total number of PNNs that persisted through mid-adolescence before increasing at P60. This decrease in female rats may indicate a difference in timing of maximal plasticity between the sexes during adolescence.

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