scholarly journals Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea

2020 ◽  
Vol 58 (3) ◽  
pp. 257-265
Author(s):  
Yeojin Park ◽  
Jinhyeong Noh ◽  
Hyun-Ji Seo ◽  
Keun-Ho Kim ◽  
Subin Min ◽  
...  

The outbreak of human toxoplasmosis can be attributed to ingestion of food contaminated with Toxoplasma gondii. Toxoplasmosis recently increased in domestic and stray dogs and cats. It prompted studies on the zoonotic infectious diseases transmitted via these animals. Sero- and antigen prevalences of T. gondii in dogs and cats were surveyed using ELISA and PCR, and B1 gene phylogeny was analyzed in this study. Toxoplasmosis antibodies were measured on sera of 403 stray cats, 947 stray dogs, 909 domestic cats, and 2,412 domestic dogs collected at nationwide regions, Korea from 2017 to 2019. In addition, whole blood, feces, and tissue samples were also collected from stray cats (1,392), stray dogs (686), domestic cats (3,040), and domestic dogs (1,974), and T. gondii-specific B1 gene PCR was performed. Antibody prevalence of stray cats, stray dogs, domestic cats, and domestic dogs were 14.1%, 5.6%, 2.3%, and 0.04%, respectively. Antigen prevalence of these animals was 0.5%, 0.2%, 0.1%, and 0.4%, respectively. Stray cats revealed the highest infection rate of toxoplasmosis, followed by stray dogs, domestic cats, and domestic dogs. B1 gene positives were 5 of stray cats, and identified to high/moderate pathogenic Type I/III group. These findings enforce that preventive hygienic measure should be strengthened at One Health level in dogs and cats, domestic and stray, to minimize human toxoplasmosis infections.

Author(s):  
Tahereh Azimpour-Ardakan ◽  
Reza Fotouhi-Ardakani ◽  
Nasser Hoghooghi-Rad ◽  
Nourdehr Rokni ◽  
Abbasali Motallebi

Background: A high correlation is observed between specific clonal lineages and host types in toxoplasmosis. The main objectives of this study were comparing polymorphism and evolutionary analysis of the B1 and ROP8 genes, as well as the evaluation of phylogenic and Toxoplasma gondii isolates obtained from different hosts and regions. Methods: Overall 96 brain/ diaphragm tissue samples of livestock and poultry from three provinces of Iran (cows: 9 from Yazd, 9 from Qom; sheep: 19 from Yazd, 7 from Qom; goats: 7 from Yazd, 4 from Qom; one camel from Yazd and 37 chickens, 2 roosters and one duck from Golestan) were tested during 2018-19. A nested PCR and PCR-PCR methods were developed with the B1 and ROP8 genes. Evaluation of genetic proximity, genetic diversity and evolutionary analysis were done using MEGA-X and DnaSP5 software. Thirty samples of both genes were sequenced (18 B1 and 12 ROP8 genes), and submitted to the GenBank (MN275903-MN275932). Results: Tajima's D index analyses showed that both genes were in the negative direction of evolution. The B1 gene was more sensitive than the ROP8 gene. The ROP8 gene showed better and more acceptable results in terms of the relationship between the host and the genotyping of the samples. Conclusion: The B1 gene was only an attractive target for rapid detection of T. gondii parasites, whereas the ROP8 gene due to a high level of polymorphism was able to isolate the three clonal lineages (type I, II and III), inter-types and even atypical strains from different isolates of T. gondii.


Parasitology ◽  
2013 ◽  
Vol 140 (14) ◽  
pp. 1768-1776 ◽  
Author(s):  
A. BURRELLS ◽  
P. M. BARTLEY ◽  
I. A. ZIMMER ◽  
S. ROY ◽  
A. C. KITCHENER ◽  
...  

SUMMARYToxoplasma gondiiis a zoonotic pathogen defined by three main clonal lineages (types I, II, III), of which type II is most common in Europe. Very few data exist on the prevalence and genotypes ofT. gondiiin the UK. Wildlife can act as sentinel species forT. gondiigenotypes present in the environment, which may subsequently be transmitted to livestock and humans. DNA was extracted from tissue samples of wild British carnivores, including 99 ferrets, 83 red foxes, 70 polecats, 65 mink, 64 badgers and 9 stoats. Parasite DNA was detected using a nested ITS1 PCR specific forT. gondii, PCR positive samples were subsequently genotyped using five PCR–RFLP markers.Toxoplasma gondiiDNA was detected within all these mammal species and prevalence varied from 6·0 to 44·4% depending on the host. PCR–RFLP genotyping identified type II as the predominant lineage, but type III and type I alleles were also identified. No atypical or mixed genotypes were identified within these animals. This study demonstrates the presence of alleles for all three clonal lineages with potential for transmission to cats and livestock. This is the first DNA-based study ofT. gondiiprevalence and genotypes across a broad range of wild British carnivores.


Viruses ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 721 ◽  
Author(s):  
Novacco ◽  
Kohan ◽  
Stirn ◽  
Meli ◽  
Díaz-Sánchez ◽  
...  

Recently, a gammaherpesvirus was described in domestic cats (FcaGHV1). The goal of the present study was to investigate the presence of FcaGHV1 in Swiss domestic cats and analyze potential risk factors. Blood samples from 881 cats presented to veterinarians in all Swiss cantons and from 91 stray cats and neoplastic tissue samples from 17 cats with lymphoma were evaluated. FcaGHV1 was detected by real-time PCR targeting the glycoprotein B gene, followed by sequencing. Blood samples were also tested for feline hemoplasmas, feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV). The molecular prevalence of FcaGHV1 was 6.0% (95% confidence interval (CI), 4.5–7.8%) in cats presented to veterinarians and 5.5% (95% CI, 1.8–12.4%) in stray cats. FcaGHV1 PCR-positive cats originated from 19/26 Swiss cantons. Factors significantly associated with FcaGHV1 detection included male sex, age >3 years, nonpedigree status and co-infection with FIV and hemoplasmas. Moreover, FeLV viremia tended to be associated with FcaGHV1 detection. High FcaGHV1 blood loads were found more frequently in FeLV-viremic cats and less frequently in hemoplasma-infected cats than in uninfected cats. Clinical information was unavailable for most of the 881 cats, but leukemia, carcinoma and cardiomyopathy were reported in FcaGHV1-positive cats. None of the tissue samples from the 17 cats with lymphoma tested positive for FcaGHV1. Sequence analyses revealed homogeneity among the Swiss isolates and >99.7% identity to published FcaGHV1 sequences. In conclusion, FcaGHV1 is present in Switzerland with a similar prevalence in cats presented to veterinarians and in stray cats. The pathogenic potential of FcaGHV1 needs further evaluation.


2009 ◽  
Vol 122 (3) ◽  
pp. 203-207 ◽  
Author(s):  
Yenisey Alfonso ◽  
Jorge Fraga ◽  
Narciso Jiménez ◽  
Carlos Fonseca ◽  
Alberto J. Dorta-Contreras ◽  
...  

2014 ◽  
Vol 23 (4) ◽  
pp. 501-508 ◽  
Author(s):  
Gislene Fátima da Silva Rocha Fournier ◽  
Marcos Gomes Lopes ◽  
Arlei Marcili ◽  
Diego Garcia Ramirez ◽  
Igor Cunha Lima Acosta ◽  
...  

Toxoplasmosis stands out as a global disease that has felines as definitive hosts. In the municipality of Natal, Rio Grande do Norte State, Brazil, two parks are notable for their ecological and social importance. This study aimed to investigate the presence of Toxoplasma gondii in short hair cats, bats and small non-volant mammals in these two ecological reserves. Altogether, biological samples were obtained from 154 mammals, 92 wild animals from both areas and 62 domestic cats of the Parque da Cidade. In total, 22 (53.7%) non-volant wild mammals, 11 (21.5%) bats and 28 (52.8%) cats were positive for IgG anti-T. gondii antibodies using the Modified Agglutination Test (≥ 25). It was possible to detect the presence of T. gondii DNA, by means of a molecular amplification of a B1 gene fragment (155bp), in 92 tissue samples from wild animals, including Didelphis albiventris, Monodelphis domestica, Artibeus lituratus, Carollia perspicillata and Glossophaga soricina. Of the 62 cats examined by the same molecular method, T. gondii DNA could be detected in 4 cats. In this study, it was observed the circulation of T. gondii in wild species and domestic cats, demonstrating the involvement of wild and domestic animals in the cycle of T. gondii.


2015 ◽  
Vol 53 (3) ◽  
pp. 259-263 ◽  
Author(s):  
Bong-Kwang Jung ◽  
Sang-Eun Lee ◽  
Hyemi Lim ◽  
Jaeeun Cho ◽  
Deok-Gyu Kim ◽  
...  

2021 ◽  
Vol 2 (2) ◽  
Author(s):  
Mahmoud Rahdar ◽  
Leila Arab ◽  
Ali Reza Samarbaf- zadeh

Toxoplasma gondii is an obligate, intracellular parasite, with worldwide distribution. The main source of infection for humans is livestock and meat-producer animals. The relationships between Toxoplasma genotype and biological characteristics of the parasite have already been identified. According to the pathogenicity of the parasite in laboratory animals, Toxoplasma is divided into three genotypes included type I, II and III. Understanding the genotype of the parasite, could help us to predict clinical features and severity of disease. The aim of this study was to identify genotypes of T. gondii in cattle and sheep meat and meat products in Ahvaz city southwest of Iran.One hundred and ninety samples of tongue, heart and muscles of sheep and cattle and meat products, including sausages and burgers, were collected from slaughterhouses and stores. To identify Toxoplasma gondii, DNA were extracted from samples and B1 gene were amplified by specific primers. To determine the genotype of T.gondii, PCR-RFLP was done on positive samples using by amplifying GRA6 gene and endonuclease Msel enzyme. Data analysis showed that the strain of the parasite in all positive samples belonged to genotype I.In this study the predominant Toxoplasma genotype was type I which can cause severe clinical symptoms in immunocompromised patients. Further research is needed to determine the genotype of the parasite in humans and other animals.


Author(s):  
Nasir AREFKHAH ◽  
Bahador SARKARI ◽  
Qasem ASGARI ◽  
Abdolali MOSHFE ◽  
Mohammad Hasan KHALAFI ◽  
...  

Background: We aimed to detect Toxoplasma gondii in ovine aborted fetuses and evaluate its genetic variations in the southwest of Iran. Methods: This cross-sectional study was performed on 100 aborted ovine fetuses collected from the different region of Kohgiluyeh and Boyer-Ahmad Province, Iran, in lambing season during 2017 and 2018. DNA was extracted from the brain samples of all of the aborted fetuses and PCR amplified, targeting a 529 bp repetitive element gene of T. gondii. Moreover, to find out the heterogeneity of the positive samples, PCR-DNA amplification of the two main genetic markers, B1 and GRA6, of T. gondii were performed. Nucleotide sequencing and phylogenetic analysis were performed, using the BLAST program and MEGA-X software. Results: The 529 bp gene of T. gondii was detected in 2 out of 100 (2%) of the ovine aborted samples. The sequences analysis of GRA6 and B1 genes revealed that both isolates from the aborted fetuses of sheep belonged to type I of T. gondii. Intra-divergence was more seen in GRA6 gene whereas less divergence was observed in B1 gene. Conclusion: Congenital infection with Type I of T. gondii during the neonatal period is associated with abortion in ovine. Evaluation of more aborted samples from broader geographical areas is needed to elucidate the molecular epidemiology and also the genotypes of T. gondii associated with abortion.


Широкое распространение безнадзорных животных на территории городов несет за собой потенциальную угрозу распространения зооантропонозных заболеваний, одним из которых является демодекоз. Невозможно разработать мероприятия, направленные на борьбу с заболеванием и его профилактику, без анализа данных особенностей возникновения и распространения инвазии среди всей популяции восприимчивых животных. Поэтому целью нашей работы явилось изучение распространения демодекоза среди безнадзорных собак и кошек в городе Тюмени. В задачи исследования входило изучение распространения демодекоза и его клинического проявления среди бездомных собак и кошек в условиях города Тюмени и определение сезонной динамики заболевания. Работу выполняли в 2016-2018 гг. на базе кафедры анатомии и физиологии ФГБОУ ВО ГАУ Северного Зауралья, в лаборатории акарологии ВНИИВЭА – филиала ТюмНЦ СО РАН, а также в производственных условиях на базе пункта временного содержания безнадзорных домашних животных МКУ «ЛесПаркХоз». Демодекозная инвазия распространена среди бездомных кошек и собак. Наиболее часто демодекоз встречается у собак, экстенсивность инвазии от 0,65 до 0,72%. Заболевание демодекозом у бездомных собак регистрировали на протяжении всего года, но 54,6% больных собак поступали в апреле и мае. Большинство больных демодекозом – это молодые собаки в возрасте от 1,5 месяцев до 2-х лет – 75,76%, животные старше двух лет гораздо реже страдали от демодекоза – 24,24%. Генерализацию демодекоза регистрировали у 21 собаки (63,64%), а локализованные очаги – у 12 собак (36,36%). Наиболее распространенной формой проявления демодекоза у бездомных собак является пустулезная, или пиодемодекоз. Данная форма заболевания была отмечена у 16 собак (48,49%), чешуйчатая форма отмечалась у 10 собак (30,30%), а смешанная – у 7 собак (21,21%). The widespread use of stray animals in urban areas carries with it the potential threat of the spread of zooanthroponotic diseases, one of which is demodicosis. It is impossible to develop measures aimed at combating the disease and its prevention without analyzing the data on the characteristics of the occurrence and spread of invasion among the entire population of susceptible animals. Therefore, the purpose of our work was to study the distribution of demodicosis among street dogs and cats in the city of Tyumen. The objectives of the study included the study of the spread of demodicosis and its clinical manifestation among stray dogs and cats in the conditions of the city of Tyumen and the determination of the seasonal dynamics of the disease. Demodectic invasion is common among stray cats and dogs. Most often, demodicosis occurs in dogs, with extensive invasion from 0.65 to 0.72%. Demodecosis in stray dogs was recorded throughout the year, but 54.6% of sick dogs were reported in April and May. The majority of patients with demodicosis are young dogs between the ages of 1.5 months and 2 years old - 75.76%, animals older than two years suffer less from demodicosis, only 24.24%. Generalization of demodicosis was recorded in 21 dogs (63.64%), and localized foci in 12 dogs (36.36%). The most common form of demodicosis in stray dogs is pustular or pyodemodecosis. This form of the disease was observed in 16 dogs (48.49%), scaly form, was observed in 10 dogs (30.30%), and mixed in 7 dogs (21.21%).


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