scholarly journals Phytochemical and acute toxicity study of aqueous extract of Bambusa vulgaris leaves on Wistar rats

2020 ◽  
Vol 02 (04) ◽  
pp. 107
Author(s):  
Appy Simone Abe ◽  
Kouao Augustin Amonkan ◽  
Kacou Marius Jules Djetouan ◽  
Kesse Philippe N’Da
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Bambusa Vulgaris

In-vitro and in-vivo evaluation of antidiabetic activity of Withania coagulans extract

2019 ◽  
Vol 09 ◽  
Author(s):  
Tejas Patel ◽  
B.N. Suhagia
Keyword(s):  
Blood Glucose ◽  
Acute Toxicity ◽  
Aqueous Extract ◽  
Pancreatic Beta Cell ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Withania Coagulans ◽  
Study Results

Background: Diabetes mellitus is major issue to public health as its prevalence is rising day by day. Synthetic agents available for the diabetic treatment are expensive or produce undesirable side effect on chronic use and some of them are not suitable during pregnancy. Herbal medicines accepted widely due to side effects and low cost. Objective: The aim of present study was to evaluate the activity of Withania coagulans extract using In-vitro and In-vivo model. Methods: Different three types of Withania coagulans extract were prepared using aqueous (W1), Alcohol (W2) and hydro-alcoholic (50:50) mixture (W3). In-vitro Anti-diabetic activity of the all three extracts evaluated using RINm5F Pancreatic beta cells.Further, n-vivo anti-diabetic evaluation performed by administering 50 mg/kg (p.o) aqueous extract for 7 days in Streptozotocin (STZ)-induced mice. Body weight of the animals was also determined to perform acute toxicity study. Results: The results of in –vitro cell based study indicated that among all three extract, aqueous extract (W1) of Withania coagulans showed potential increase in inulin release. The EC50 of the W1 (249.6 µg/L) which is compared with standard (Glibenclamide) EC50. From the results of In-vitro study, W1 subjected for acute toxicity study and the acute toxicity study results indicated LD50 of 50mg/kg. Diabetic rats treated with W1 extract at oral dose of 50 mg/kg for 7 days showed 34.17% reduction in blood glucose in comparison to untreated diabetic (STZ-induced) rats. Blood glucose levels of Standard treated (Glibenclamide) and control untreated. Conclusion: In conclusion, results of pancreatic beta cell based study showed increase in insulin release by administration of extract. Further aqueous extract (W1) was potentially reduced blood glucose level in STZ induced diabetic mice.

scholarly journals Acute Toxicity Study of Standardized Mitragyna speciosa Korth Aqueous Extract in Sprague Dawley Rats

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Mohd Saleh Ahmad Kamal ◽  
Ahmad Rohi Ghazali ◽  
Noral ‘Ashikin Yahya ◽  
Mohd Isa Wasiman ◽  
Zakiah Ismail
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Toxicity Study ◽  
Sprague Dawley ◽  
Mitragyna Speciosa ◽  
Acute Toxicity Study ◽  
Sprague Dawley Rats

scholarly journals Antidiarrhoeal activity of aqueous leaf extract of Olea europaea var. sylvestris in albino Wistar rats

2018 ◽  
Vol 91 (2) ◽  
Author(s):  
Mohamed Zaouani ◽  
Fatima Yahiaoui ◽  
Nazli Nacer Bey ◽  
Meriem Hind Ben-Mahdi
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Aqueous Extract ◽  
Olea Europaea ◽  
Castor Oil ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Motility Assay ◽  
Phytochemical Screening ◽  
Leaf Aqueous Extract

Olea europaea var. sylvestris, also named oleaster, is widely used by traditional medicine practitioners in Algeria to treat high blood pressure and diabetes. However, the antidiarrhoeal activity of this plant has not been scientifically evaluated. The main aim of the study deals with an investigation of three topics: the phytochemical screening, the acute toxicity, and antidiarrhoeal activity of the oleaster leaf aqueous extract. Acute oral toxicity study was carried out based on Organization for Economic Cooperation and Development 423 guideline. The extract was orally administered in wistar rats at a single dose of 2000 mg/kg body weight and the animals were observed for mortality, behavioral changes and other abnormal signs. Qualitative analysis of phytochemical constituents was carried out using standard methods developed by Harborne, Trease and Evans. Castor oil-induced diarrhoea tests and gastro intestinal motility assay were evaluated in rats to determine the antidiarrhoeal activity of the extract. In the acute toxicity study, the extract did not induce death or any sign of toxicity in treated rats. The preliminary phytochemical screening of the extract revealed the presence of saponins, flavonoids, and triterpenoids. The oleaster extract at oral doses of 100, 200 and 400 mg/kg body weight showed a significant (P<0.05) antidiarrhoeal activity compared to the control group treated with castor oil induced diarrhoea, enteropooling and gastrointestinal motility assay, after charcoal meal administration. The oleaster leaf aqueous extract has shown a gradual response with increasing dose. The present study indicates that the oleaster leaf aqueous extract is safe with antidiarrhoeal property.

Sub-acute toxicity study on the aqueous extract of Albizia zygia stem bark

2016 ◽  
Vol 13 (1) ◽  
pp. 32 ◽  
Author(s):  
Stephen O. Okpo ◽  
Clare O. Igwealor ◽  
Gerald I. Eze
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Stem Bark ◽  
Toxicity Study ◽  
Acute Toxicity Study

Acute Toxicity Study of Herbal Ovulation Inducer Product ‘Estrofarm’ in Wistar Rats

2011 ◽  
Vol 4 (5) ◽  
pp. 528-529
Author(s):  
M. M. Gatne M. M. Gatne ◽  
◽  
M.J Saxena M.J Saxena ◽  
K. Ravikanth K. Ravikanth ◽  
A. Thakur A. Thakur
Keyword(s):  
Acute Toxicity ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Acute Toxicity Study

scholarly journals ANTIHYPERLIPIDEMIC AND HEPATOPROTECTIVE STUDIES ON LEAVES OF MACARANGA TANARIUS

2016 ◽  
Vol 10 (1) ◽  
pp. 239
Author(s):  
Phebe Hendra ◽  
Fenty Fenty ◽  
Jeffry Julianus ◽  
Oktariani Aurelia Jamil ◽  
Dian Ayu Maharani ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Toxicity Study ◽  
Lipoprotein Cholesterol ◽  
Acute Toxicity Study ◽  
Macaranga Tanarius ◽  
Serum Glutamate ◽  
Ethanol Fraction

ABSTRACTObjective: This study investigated the antihyperlipidemic and hepatoprotective effects of the hexane-ethanol fraction of methanol extract ofMacaranga tanarius (HEM) in rats.Methods: The hexane-ethanol fraction was screened for toxicity by oral acute toxicity study. The antihyperlipidemic effect of the hexane-ethanolfraction and the unsolved of the hexane-ethanol fraction is measured against Wistar rats induced by glucose-fructose diets for 42 days throughmeasuring serum cholesterol, triglyceride (TG), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), and fastingblood glucose. The hepatoprotective effect of the hexane-ethanol fraction is determined against Wistar rats with liver damage induced by carbontetrachloride through measuring serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkalinephosphatase (ALP), albumin, lactate dehydrogenase (LDH), and total bilirubin.Results: There is no toxic effect that was observed on acute toxicity study. The TG, LDL-c, and fasting blood glucose levels were significantly (p<0.05)reduced after both of treatment the hexane-ethanol fraction and the unsolved HEM. Administration of the hexane-ethanol fraction 68.6 mg/kgBWsignificantly (p<0.05) prevented elevation of SGPT, SGOT, LDH, ALP, and decreasing of albumin level.Conclusion: The study showed antihyperlipidemic and hepatoprotective activities of the HEM in animal models.Keywords: Macaranga tanarius, Antihyperlipidemic, Hepatoprotective.

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