Acute Toxicity Study of Herbal Ovulation Inducer Product ‘Estrofarm’ in Wistar Rats

2011 ◽  
Vol 4 (5) ◽  
pp. 528-529
Author(s):  
M. M. Gatne M. M. Gatne ◽  
◽  
M.J Saxena M.J Saxena ◽  
K. Ravikanth K. Ravikanth ◽  
A. Thakur A. Thakur
Keyword(s):  
Acute Toxicity ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Acute Toxicity Study

scholarly journals ANTIHYPERLIPIDEMIC AND HEPATOPROTECTIVE STUDIES ON LEAVES OF MACARANGA TANARIUS

2016 ◽  
Vol 10 (1) ◽  
pp. 239
Author(s):  
Phebe Hendra ◽  
Fenty Fenty ◽  
Jeffry Julianus ◽  
Oktariani Aurelia Jamil ◽  
Dian Ayu Maharani ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Toxicity Study ◽  
Lipoprotein Cholesterol ◽  
Acute Toxicity Study ◽  
Macaranga Tanarius ◽  
Serum Glutamate ◽  
Ethanol Fraction

ABSTRACTObjective: This study investigated the antihyperlipidemic and hepatoprotective effects of the hexane-ethanol fraction of methanol extract ofMacaranga tanarius (HEM) in rats.Methods: The hexane-ethanol fraction was screened for toxicity by oral acute toxicity study. The antihyperlipidemic effect of the hexane-ethanolfraction and the unsolved of the hexane-ethanol fraction is measured against Wistar rats induced by glucose-fructose diets for 42 days throughmeasuring serum cholesterol, triglyceride (TG), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), and fastingblood glucose. The hepatoprotective effect of the hexane-ethanol fraction is determined against Wistar rats with liver damage induced by carbontetrachloride through measuring serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkalinephosphatase (ALP), albumin, lactate dehydrogenase (LDH), and total bilirubin.Results: There is no toxic effect that was observed on acute toxicity study. The TG, LDL-c, and fasting blood glucose levels were significantly (p<0.05)reduced after both of treatment the hexane-ethanol fraction and the unsolved HEM. Administration of the hexane-ethanol fraction 68.6 mg/kgBWsignificantly (p<0.05) prevented elevation of SGPT, SGOT, LDH, ALP, and decreasing of albumin level.Conclusion: The study showed antihyperlipidemic and hepatoprotective activities of the HEM in animal models.Keywords: Macaranga tanarius, Antihyperlipidemic, Hepatoprotective.

scholarly journals Phytochemical and acute toxicity study of aqueous extract of Bambusa vulgaris leaves on Wistar rats

2020 ◽  
Vol 02 (04) ◽  
pp. 107
Author(s):  
Appy Simone Abe ◽  
Kouao Augustin Amonkan ◽  
Kacou Marius Jules Djetouan ◽  
Kesse Philippe N’Da
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Bambusa Vulgaris

scholarly journals Inhibitory Activity of Boesenbergia rotunda (L.) Mansf. Rhizome towards the Expression of Akt and NF-KappaB p65 in Acetic Acid-Induced Wistar Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Aziiz Mardanarian Rosdianto ◽  
Irma Melyani Puspitasari ◽  
Ronny Lesmana ◽  
Jutti Levita
Keyword(s):  
Acetic Acid ◽  
Acute Toxicity ◽  
Normal Control ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Mechanism Study ◽  
Acute Toxicity Study ◽  
Inflammatory Mechanism ◽  
Anti Inflammatory ◽  
Nf Kappab

Ethnopharmacological Relevance. Boesenbergia rotunda has been traditionally used to reduce stomach discomfort in Indonesia. The objective of the present study was to investigate the anti-inflammatory mechanism and acute toxicity of the ethanol extract of B. rotunda rhizome (BREE) in Wistar rats. Materials and Methods. Forty-eight male Wistar rats were divided into anti-inflammatory mechanism study (n = 18) and acute toxicity study (n = 30). The anti-inflammatory mechanism study employed six groups (n = 3), e.g., the normal control, negative control, positive control (quercetin 20 mg/kg BW), and three doses of BREE (250 mg/kg BW; 500 mg/kg BW; 1000 mg/kg BW). All groups (except the normal control) were inflammatory-induced i.p. using 0.1 mL of 1% of acetic acid. The expression of Akt and NF-kappaB p65 in the stomach and intestine of the rats was examined using Western blot analysis. The acute toxicity study (21 days) was conducted by following the Regulation of Indonesia National Agency of Drug and Food Control No. 7/2014 about In Vivo Nonclinical Toxicity Study using 5 doses of BREE (250 mg/kg BW; 500 mg/kg BW; 1000 mg/kg BW; 2000 mg/kg BW; 4000 mg/kg BW). Results. BREE reduces the infiltration of inflammatory cells in both the stomach and the intestine of acetic acid-induced rats. BREE also alters the expression of Akt and NF-kappaB p65 in the rat’s stomach and intestine (p=0.005). The acute toxicity study reveals no lethal effects and behavioral signs of toxicity at all tested doses, which indicates that the LD50 is greater than 4000 mg/kg BW. Conclusion. Taken together, BREE could inhibit the expression of Akt and NF-kappaB p65 in the stomach and intestine of acetic acid-induced Wistar rats. This plant could be further explored for its potential as plant-based antistomach ulceration.

scholarly journals Acute toxicity study of seeds of Achyranthes aspera, bark of Berberis aristata and roots of Coleus forskohlii in Wistar rats

2020 ◽  
Vol 9 (9) ◽  
pp. 1424
Author(s):  
J. Ravi Kumar ◽  
V. Prasanna ◽  
Chakradhar . ◽  
K. C. Haritha
Keyword(s):  
Acute Toxicity ◽  
Cell Biology ◽  
Wistar Rats ◽  
Clinical Signs ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Toxicity Study ◽  
Achyranthes Aspera ◽  
Acute Toxicity Study ◽  
The Family

Background: Achyranthes aspera is a species of plant in the family Amaranthaceae. Berberis aristata is a shrub belonging to the family Berberidaceae and the genus Berberis. Plectranthus barbatus is a tropical perennial plant related to the typical coleus species. It produces forskolin, an extract useful for pharmaceutical preparations and research in cell biology. It is belonging to Lamiaceae. The present study has been undertaken to study the toxic effects of hydro alcoholic extracts of A. aspera, B. aristata, C. forskohlii in albino Wistar rats and to establish the hazardous safety category of hydro alcoholic extracts of these plants as per organization for economic cooperation and development (OECD-423) guidelines and GHS classification system respectively.Methods: In acute toxicity study, the hydro-alcoholic extracts of all the above three plants were given orally at the dose of 2000 mg/kg b. w. to three rats in each group respectively in step I. Then, all the animals were observed for initial 4 hours and followed by fourteen days for their clinical signs and mortality in step II.Results: In step I, all the animals were normal and there was no mortality after 48 hours. In step II with the same dose, all the animals showed no adverse effects and no mortality when followed up to 14 days observation period.Conclusions: The result indicates that the hydro alcoholic extracts of A. aspera, B. aristata, C. forskohlii plants can be utilized safely for therapeutic use in pharmaceutical formulations and it falls under category ‘5’ or ‘unclassified’ of GHS system.

scholarly journals Acute toxicity study of the ethanolic extract of Eleutherine bulbosa Urb in Wistar rats

2021 ◽  
pp. 143-147
Author(s):  
Helmina Wati ◽  
Rahmi Muthia ◽  
Kartini ◽  
Finna Setiawan
Keyword(s):  
Acute Toxicity ◽  
Toxicity Test ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Test Study ◽  
Limit Test ◽  
Low Toxicity ◽  
Medicinal Properties

Introduction: Eleutherine bulbosa Urb is a plant species with medicinal properties, including anti-inflammatory, widely relied upon in traditional practices. For this reason, the present research was intended to assess and, thus, ensure the safety of this plant for conventional medicinal purposes using a toxicity test study. Methods: The acute toxicity test of the ethanolic extract of E. bulbosa Urb (EEEB) used the method adopted from the Organization Economic Cooperation and Development (OECD) guidelines 425 for testing Wistar rats. Results: During 14 days of the acute toxicity study, there were no significant changes in rat weight, no mortality, and no signs of toxicity after the oral EEEB administration at 2000 mg/kg body weight (bw). The limit test showed that the LD50 of EEEB was higher than 2000 mg/kg bw. Conclusion: EEEB has low toxicity because its LD50 is higher than the limit test results.

PHARMACOGNOSTIC, ANTIOXIDANT AND ACUTE TOXICITY STUDY OF Ficus sycomorus (Linn) (Moraceae) ROOT AND STEM BARK

2020 ◽  
Vol 4 (2) ◽  
pp. 605-614
Author(s):  
Murtala M. Namadina ◽  
H. Haruna ◽  
U. Sanusi
Keyword(s):  
Acute Toxicity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
The Body ◽  
Toxicity Study ◽  
Bark Extract ◽  
Root Extract ◽  
Acute Toxicity Study ◽  
Radical Scavenging Ability ◽  
Phytochemical Components

Most of biochemical reactions in the body generates Reactive Oxygen Species (ROS), which are involved in the pathogenesis of oxidative stress-related disorders like diabetes, nephrotoxicity, cancer, cardiovascular disorders, inflammation and neurological disorders when they attack biochemical molecules like proteins, lipids and nucleic acid. Antioxidants are used to protect the cells or tissues against potential attack by ROS. Most medicinal plants possess a rich source of antioxidants such as flavonoids, phenols, tannins, alkaloids among others. These phytochemicals are currently pursued as an alternative and complimentary drug. In this study, phytochemical components, antioxidant and acute toxicity study of the methanol extract of stem bark and root of F. sycomorus were carried out using standard methods. Findings from this study revealed the presence of some diagnostic microscopical features such as calcium oxalate, starch, gum/mucilage, lignin, Aleurone grain, suberized/Cuticular cell wall and inulin but calcium carbonate was absent in stem bark but present in the powdered root. Quantitative physical constants include moisture contents (6.40% and 7.82%), ash value (7.20% and 9.30 %) in stem bark and root respectively. Carbohydrates, alkaloid, flavonoids, saponins, tannins, glycoside, steroid, triterpenes and phenols were present in all the extracts. They were found to exhibit potent 1,1,-diphenyl 2-picryl hydrazyl (DPPH) free scavenging activity. The DPPH radical scavenging ability of the extracts showed the following trend Ascorbic acid < stem bark extract˃ root extract. The LD50 of the methanolic stem bark and root extracts were found to be greater than 5000 mg /kg and is considered safe for use. Nonetheless, further

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