Acute toxicity study of the crude extract of the fruit rind of rambutan (Nephelium lappaceum L.) in male Wistar rats

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
A Thinkratok ◽  
R Srisawat
Keyword(s):  
Acute Toxicity ◽  
Crude Extract ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Nephelium Lappaceum ◽  
Male Wistar Rats ◽  
Fruit Rind

scholarly journals Short Communication: Acute toxicity study of plantaricin from Lactobacillus plantarum S34 and its antibacterial activity

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Arido Yugovelman Ahaddin ◽  
Sri Budiarti ◽  
Apon Zaenal Mustopa ◽  
Huda S Darusman ◽  
Lita Triratna
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Acute Toxicity ◽  
Lactobacillus Plantarum ◽  
Crude Extract ◽  
Short Communication ◽  
Histopathological Examination ◽  
Blood Analysis ◽  
Toxicity Study ◽  
Acute Toxicity Study

Abstract. Ahaddin AY, Budiarti S, Mustopa AZ, Darusman HS, Triratna L. 2021. Short Communication: Acute toxicity study of plantaricin from Lactobacillus plantarum S34 and its antibacterial activity. Biodiversitas 22: 227-232. Lactobacillus plantarum S34 belongs to the Lactobacilli class produces a bacteriocin called plantaricin. Bacteriocins are well known as active compounds that inhibit bacterial growth. This study was conducted to determine the antimicrobial activity of plantaricin S34 and its safety profile in the ddY mouse animal models. Plantaricin 34 from the crude extract was identified using Tricine SDS-PAGE. Antimicrobial activity was observed using disk diffusion against EPEC K1.1, S. aureus, S. typhosa, S typhimurium, and Proteus sp. The safety assessment showed that crude extract of plantaricin S34 did not cause any abnormalities to experimental mice even after being administrated with 5000 mg/kg BB. The identification of plantaricin S34 showed an active molecule at 7.34 kDa and had an activity to inhibit the pathogens used in this study. The blood analysis showed that the hematological and biochemical blood properties were in the normal range. Histopathological examination showed no damage to the intestine, liver, and kidneys. Thus, the crude extract of plantaricin S34 is active as an antimicrobial agent without any toxicity effects.

Acute Toxicity Study of Herbal Ovulation Inducer Product ‘Estrofarm’ in Wistar Rats

2011 ◽  
Vol 4 (5) ◽  
pp. 528-529
Author(s):  
M. M. Gatne M. M. Gatne ◽  
◽  
M.J Saxena M.J Saxena ◽  
K. Ravikanth K. Ravikanth ◽  
A. Thakur A. Thakur
Keyword(s):  
Acute Toxicity ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Acute Toxicity Study

scholarly journals ANTIHYPERLIPIDEMIC AND HEPATOPROTECTIVE STUDIES ON LEAVES OF MACARANGA TANARIUS

2016 ◽  
Vol 10 (1) ◽  
pp. 239
Author(s):  
Phebe Hendra ◽  
Fenty Fenty ◽  
Jeffry Julianus ◽  
Oktariani Aurelia Jamil ◽  
Dian Ayu Maharani ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Toxicity Study ◽  
Lipoprotein Cholesterol ◽  
Acute Toxicity Study ◽  
Macaranga Tanarius ◽  
Serum Glutamate ◽  
Ethanol Fraction

ABSTRACTObjective: This study investigated the antihyperlipidemic and hepatoprotective effects of the hexane-ethanol fraction of methanol extract ofMacaranga tanarius (HEM) in rats.Methods: The hexane-ethanol fraction was screened for toxicity by oral acute toxicity study. The antihyperlipidemic effect of the hexane-ethanolfraction and the unsolved of the hexane-ethanol fraction is measured against Wistar rats induced by glucose-fructose diets for 42 days throughmeasuring serum cholesterol, triglyceride (TG), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), and fastingblood glucose. The hepatoprotective effect of the hexane-ethanol fraction is determined against Wistar rats with liver damage induced by carbontetrachloride through measuring serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkalinephosphatase (ALP), albumin, lactate dehydrogenase (LDH), and total bilirubin.Results: There is no toxic effect that was observed on acute toxicity study. The TG, LDL-c, and fasting blood glucose levels were significantly (p<0.05)reduced after both of treatment the hexane-ethanol fraction and the unsolved HEM. Administration of the hexane-ethanol fraction 68.6 mg/kgBWsignificantly (p<0.05) prevented elevation of SGPT, SGOT, LDH, ALP, and decreasing of albumin level.Conclusion: The study showed antihyperlipidemic and hepatoprotective activities of the HEM in animal models.Keywords: Macaranga tanarius, Antihyperlipidemic, Hepatoprotective.

scholarly journals Evaluation of Sub-acute and Sub Chronic Toxicity Profile of the Aqueous Leaf Crude Extract of Melanthera Scandens (Schumach & Thonn) in Wistar Rats

2019 ◽  
pp. 1-16
Author(s):  
Daniel Chans Mwandah ◽  
Ibrahim Ntulume ◽  
Adamu Almustapha Aliero ◽  
Kennedy Kiyimba ◽  
Emmanuel Tiyo Ayikobua ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Crude Extract ◽  
Wistar Rats ◽  
Control Group ◽  
Subchronic Toxicity ◽  
Significant Treatment ◽  
Study Results ◽  
Need To Evaluate ◽  
Female Wistar Rats ◽  
Male Wistar Rats

Aims: Although Melanthera scandens is a plant widely used in traditional medicine for the management of seizures, stomach ulcers and sores, dysmenorrhea, diabetes and malaria, there was scanty information about its safety. There was, therefore, a need to evaluate the sub-acute and subchronic toxicity studies of this plant which would reflect on its safety. Methodology: This was an experimental laboratory study. The research was conducted at Kampala International University-Western Campus at the Pharmacology laboratory from February to June 2017. The sub-acute toxicity was evaluated after administering daily oral doses of M. scandens crude extract (250, 500 and 1000 mg/kg) for 28 days and 90 days for subchronic study, after which the effect on haematological, biochemical and histopathological parameters were assessed in male and female Wistar rats (five of each sex). Results: Sub-acute toxicity results revealed that there was a significant decrease in the AST between the male Wistar rats that received 250 mg/kg (P= .005) and those that received 500 mg/kg (P= .05) as compared with the control group. Subchronic studies showed a significant increase in ALP (P= .05) at 1000 mg/kg compared with 500 mg/kg. Terminal necropsy did not reveal any treatment-related histopathological findings. There were also no toxicologically significant treatment-related effects on haematological parameters. The sub-acute toxicity results suggest that doses of 250mg/kg and 500mg/kg are safe and could be hepatoprotective due to reduced levels of AST and ALP, while the subchronic toxicity study results suggest that doses greater than 1000 mg/kg could be toxic to the plasma membrane, liver cells or endoplasmic reticulum due to increased ALP levels at this dose. Conclusion: The M. scandens crude extract did not cause significant toxicity on haematological and histopathological indices, after sub-acute and subchronic administration in Wistar rats.

scholarly journals Phytochemical and acute toxicity study of aqueous extract of Bambusa vulgaris leaves on Wistar rats

2020 ◽  
Vol 02 (04) ◽  
pp. 107
Author(s):  
Appy Simone Abe ◽  
Kouao Augustin Amonkan ◽  
Kacou Marius Jules Djetouan ◽  
Kesse Philippe N’Da
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Bambusa Vulgaris

scholarly journals Inhibitory Activity of Boesenbergia rotunda (L.) Mansf. Rhizome towards the Expression of Akt and NF-KappaB p65 in Acetic Acid-Induced Wistar Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Aziiz Mardanarian Rosdianto ◽  
Irma Melyani Puspitasari ◽  
Ronny Lesmana ◽  
Jutti Levita
Keyword(s):  
Acetic Acid ◽  
Acute Toxicity ◽  
Normal Control ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Mechanism Study ◽  
Acute Toxicity Study ◽  
Inflammatory Mechanism ◽  
Anti Inflammatory ◽  
Nf Kappab

Ethnopharmacological Relevance. Boesenbergia rotunda has been traditionally used to reduce stomach discomfort in Indonesia. The objective of the present study was to investigate the anti-inflammatory mechanism and acute toxicity of the ethanol extract of B. rotunda rhizome (BREE) in Wistar rats. Materials and Methods. Forty-eight male Wistar rats were divided into anti-inflammatory mechanism study (n = 18) and acute toxicity study (n = 30). The anti-inflammatory mechanism study employed six groups (n = 3), e.g., the normal control, negative control, positive control (quercetin 20 mg/kg BW), and three doses of BREE (250 mg/kg BW; 500 mg/kg BW; 1000 mg/kg BW). All groups (except the normal control) were inflammatory-induced i.p. using 0.1 mL of 1% of acetic acid. The expression of Akt and NF-kappaB p65 in the stomach and intestine of the rats was examined using Western blot analysis. The acute toxicity study (21 days) was conducted by following the Regulation of Indonesia National Agency of Drug and Food Control No. 7/2014 about In Vivo Nonclinical Toxicity Study using 5 doses of BREE (250 mg/kg BW; 500 mg/kg BW; 1000 mg/kg BW; 2000 mg/kg BW; 4000 mg/kg BW). Results. BREE reduces the infiltration of inflammatory cells in both the stomach and the intestine of acetic acid-induced rats. BREE also alters the expression of Akt and NF-kappaB p65 in the rat’s stomach and intestine (p=0.005). The acute toxicity study reveals no lethal effects and behavioral signs of toxicity at all tested doses, which indicates that the LD50 is greater than 4000 mg/kg BW. Conclusion. Taken together, BREE could inhibit the expression of Akt and NF-kappaB p65 in the stomach and intestine of acetic acid-induced Wistar rats. This plant could be further explored for its potential as plant-based antistomach ulceration.

scholarly journals Acute toxicity study of seeds of Achyranthes aspera, bark of Berberis aristata and roots of Coleus forskohlii in Wistar rats

2020 ◽  
Vol 9 (9) ◽  
pp. 1424
Author(s):  
J. Ravi Kumar ◽  
V. Prasanna ◽  
Chakradhar . ◽  
K. C. Haritha
Keyword(s):  
Acute Toxicity ◽  
Cell Biology ◽  
Wistar Rats ◽  
Clinical Signs ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Toxicity Study ◽  
Achyranthes Aspera ◽  
Acute Toxicity Study ◽  
The Family

Background: Achyranthes aspera is a species of plant in the family Amaranthaceae. Berberis aristata is a shrub belonging to the family Berberidaceae and the genus Berberis. Plectranthus barbatus is a tropical perennial plant related to the typical coleus species. It produces forskolin, an extract useful for pharmaceutical preparations and research in cell biology. It is belonging to Lamiaceae. The present study has been undertaken to study the toxic effects of hydro alcoholic extracts of A. aspera, B. aristata, C. forskohlii in albino Wistar rats and to establish the hazardous safety category of hydro alcoholic extracts of these plants as per organization for economic cooperation and development (OECD-423) guidelines and GHS classification system respectively.Methods: In acute toxicity study, the hydro-alcoholic extracts of all the above three plants were given orally at the dose of 2000 mg/kg b. w. to three rats in each group respectively in step I. Then, all the animals were observed for initial 4 hours and followed by fourteen days for their clinical signs and mortality in step II.Results: In step I, all the animals were normal and there was no mortality after 48 hours. In step II with the same dose, all the animals showed no adverse effects and no mortality when followed up to 14 days observation period.Conclusions: The result indicates that the hydro alcoholic extracts of A. aspera, B. aristata, C. forskohlii plants can be utilized safely for therapeutic use in pharmaceutical formulations and it falls under category ‘5’ or ‘unclassified’ of GHS system.

scholarly journals Acute toxicity study of the ethanolic extract of Eleutherine bulbosa Urb in Wistar rats

2021 ◽  
pp. 143-147
Author(s):  
Helmina Wati ◽  
Rahmi Muthia ◽  
Kartini ◽  
Finna Setiawan
Keyword(s):  
Acute Toxicity ◽  
Toxicity Test ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Test Study ◽  
Limit Test ◽  
Low Toxicity ◽  
Medicinal Properties

Introduction: Eleutherine bulbosa Urb is a plant species with medicinal properties, including anti-inflammatory, widely relied upon in traditional practices. For this reason, the present research was intended to assess and, thus, ensure the safety of this plant for conventional medicinal purposes using a toxicity test study. Methods: The acute toxicity test of the ethanolic extract of E. bulbosa Urb (EEEB) used the method adopted from the Organization Economic Cooperation and Development (OECD) guidelines 425 for testing Wistar rats. Results: During 14 days of the acute toxicity study, there were no significant changes in rat weight, no mortality, and no signs of toxicity after the oral EEEB administration at 2000 mg/kg body weight (bw). The limit test showed that the LD50 of EEEB was higher than 2000 mg/kg bw. Conclusion: EEEB has low toxicity because its LD50 is higher than the limit test results.

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