scholarly journals Anthelmintic potential and in vivo acute toxicity study of Piptadenia gonoachanta leaf aqueous extract

2017 ◽  
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Leaf Aqueous Extract

In-vitro and in-vivo evaluation of antidiabetic activity of Withania coagulans extract

2019 ◽  
Vol 09 ◽  
Author(s):  
Tejas Patel ◽  
B.N. Suhagia
Keyword(s):  
Blood Glucose ◽  
Acute Toxicity ◽  
Aqueous Extract ◽  
Pancreatic Beta Cell ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Withania Coagulans ◽  
Study Results

Background: Diabetes mellitus is major issue to public health as its prevalence is rising day by day. Synthetic agents available for the diabetic treatment are expensive or produce undesirable side effect on chronic use and some of them are not suitable during pregnancy. Herbal medicines accepted widely due to side effects and low cost. Objective: The aim of present study was to evaluate the activity of Withania coagulans extract using In-vitro and In-vivo model. Methods: Different three types of Withania coagulans extract were prepared using aqueous (W1), Alcohol (W2) and hydro-alcoholic (50:50) mixture (W3). In-vitro Anti-diabetic activity of the all three extracts evaluated using RINm5F Pancreatic beta cells.Further, n-vivo anti-diabetic evaluation performed by administering 50 mg/kg (p.o) aqueous extract for 7 days in Streptozotocin (STZ)-induced mice. Body weight of the animals was also determined to perform acute toxicity study. Results: The results of in –vitro cell based study indicated that among all three extract, aqueous extract (W1) of Withania coagulans showed potential increase in inulin release. The EC50 of the W1 (249.6 µg/L) which is compared with standard (Glibenclamide) EC50. From the results of In-vitro study, W1 subjected for acute toxicity study and the acute toxicity study results indicated LD50 of 50mg/kg. Diabetic rats treated with W1 extract at oral dose of 50 mg/kg for 7 days showed 34.17% reduction in blood glucose in comparison to untreated diabetic (STZ-induced) rats. Blood glucose levels of Standard treated (Glibenclamide) and control untreated. Conclusion: In conclusion, results of pancreatic beta cell based study showed increase in insulin release by administration of extract. Further aqueous extract (W1) was potentially reduced blood glucose level in STZ induced diabetic mice.

scholarly journals Gastroprotective Effects of Lion’s Mane MushroomHericium erinaceus(Bull.:Fr.) Pers. (Aphyllophoromycetideae) Extract against Ethanol-Induced Ulcer in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Jing-Yang Wong ◽  
Mahmood Ameen Abdulla ◽  
Jegadeesh Raman ◽  
Chia-Wei Phan ◽  
Umah Rani Kuppusamy ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Gastric Wall ◽  
Toxicity Study ◽  
Control Group ◽  
High Dose ◽  
Sprague Dawley ◽  
Bax Protein ◽  
Acute Toxicity Study

Hericium erinaceusis a famous tonic in oriental medicine. The gastroprotective effects of aqueous extract ofH. erinaceusagainst ethanol-induced ulcers inSprague Dawleyrats were investigated. The possible involvements of lipid peroxidation, superoxide dismutase, and catalase were also investigated. Acute toxicity study was performed. The effects of aqueous extract ofH. erinaceuson the ulcer areas, ulcer inhibition, gastric wall mucus, gross and histological gastric lesions, antioxidant levels, and malondialdehyde (MDA) contents were evaluated in ethanol-induced ulcerin vivo. In acute toxicity study, a high dose of 5 g/kg did not manifest any toxicological signs in rats. The extract promoted ulcer protection as ascertained by a significant reduction of the ulcer area. Furthermore, it exhibited a significant protection activity against gastric mucosal injury by preventing the depletion of antioxidant enzymes. The level of MDA was also limited in rat stomach tissues when compared with the ulcer control group. Immunohistochemistry showed upregulation of HSP70 protein and downregulation of BAX protein in rats pretreated with the extract. The aqueous extract ofH. erinaceusprotected gastric mucosa in ourin vivomodel. It is speculated that the bioactive compounds present in the extract may play a major role in gastroprotective activity.

scholarly journals Acute Toxicity Study of Standardized Mitragyna speciosa Korth Aqueous Extract in Sprague Dawley Rats

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Mohd Saleh Ahmad Kamal ◽  
Ahmad Rohi Ghazali ◽  
Noral ‘Ashikin Yahya ◽  
Mohd Isa Wasiman ◽  
Zakiah Ismail
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Toxicity Study ◽  
Sprague Dawley ◽  
Mitragyna Speciosa ◽  
Acute Toxicity Study ◽  
Sprague Dawley Rats

scholarly journals Antidiarrhoeal activity of aqueous leaf extract of Olea europaea var. sylvestris in albino Wistar rats

2018 ◽  
Vol 91 (2) ◽  
Author(s):  
Mohamed Zaouani ◽  
Fatima Yahiaoui ◽  
Nazli Nacer Bey ◽  
Meriem Hind Ben-Mahdi
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Aqueous Extract ◽  
Olea Europaea ◽  
Castor Oil ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Motility Assay ◽  
Phytochemical Screening ◽  
Leaf Aqueous Extract

Olea europaea var. sylvestris, also named oleaster, is widely used by traditional medicine practitioners in Algeria to treat high blood pressure and diabetes. However, the antidiarrhoeal activity of this plant has not been scientifically evaluated. The main aim of the study deals with an investigation of three topics: the phytochemical screening, the acute toxicity, and antidiarrhoeal activity of the oleaster leaf aqueous extract. Acute oral toxicity study was carried out based on Organization for Economic Cooperation and Development 423 guideline. The extract was orally administered in wistar rats at a single dose of 2000 mg/kg body weight and the animals were observed for mortality, behavioral changes and other abnormal signs. Qualitative analysis of phytochemical constituents was carried out using standard methods developed by Harborne, Trease and Evans. Castor oil-induced diarrhoea tests and gastro intestinal motility assay were evaluated in rats to determine the antidiarrhoeal activity of the extract. In the acute toxicity study, the extract did not induce death or any sign of toxicity in treated rats. The preliminary phytochemical screening of the extract revealed the presence of saponins, flavonoids, and triterpenoids. The oleaster extract at oral doses of 100, 200 and 400 mg/kg body weight showed a significant (P<0.05) antidiarrhoeal activity compared to the control group treated with castor oil induced diarrhoea, enteropooling and gastrointestinal motility assay, after charcoal meal administration. The oleaster leaf aqueous extract has shown a gradual response with increasing dose. The present study indicates that the oleaster leaf aqueous extract is safe with antidiarrhoeal property.

Sub-acute toxicity study on the aqueous extract of Albizia zygia stem bark

2016 ◽  
Vol 13 (1) ◽  
pp. 32 ◽  
Author(s):  
Stephen O. Okpo ◽  
Clare O. Igwealor ◽  
Gerald I. Eze
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Stem Bark ◽  
Toxicity Study ◽  
Acute Toxicity Study

Amphiphilic poly-N-vinylpyrrolidone nanoparticles as carriers for non-steroidal, anti-inflammatory drugs: In vitro cytotoxicity and in vivo acute toxicity study

2017 ◽  
Vol 13 (3) ◽  
pp. 1021-1030 ◽  
Author(s):  
Andrey N. Kuskov ◽  
Pavel P. Kulikov ◽  
Anastasia V. Goryachaya ◽  
Manolis N. Tzatzarakis ◽  
Anca O. Docea ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
In Vitro Cytotoxicity ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

scholarly journals In vivo antiplasmodial potential of aqueous seed extract of Ricinus communis

2019 ◽  
Vol 8 (2) ◽  
pp. 133-138
Author(s):  
Peace ME. Ubulom ◽  
Ette O. Ettebong ◽  
Edidiong J. Udofia ◽  
Rachel S Inyang Etuk
Keyword(s):  
Acute Toxicity ◽  
Ricinus Communis ◽  
Lethal Dose ◽  
Suppressive Effect ◽  
Seed Extract ◽  
Toxicity Study ◽  
Control Group ◽  
Active Principle ◽  
Acute Toxicity Study

Introduction: Ricinus communis is used by the people of Niger-Delta region of Nigeria, for the treatment of various ailments, especially malaria. This study evaluated the antiplasmodial potentials of the aqueous seed extract of R. communis, using Plasmodium berghei berghei. Methods: Acute toxicity study was carried out to determine the median lethal dose (LD50) of the extract. Antiplasmodial effect of the extract was assessed in suppressive, repository/ prophylactic and curative models, using Swiss albino mice (15-29 g). Mice were infected intraperitoneally with 0.2 mL of parasitized blood. Extract doses administered were 54.77, 109.54 and 164.32 mg/kg/d of the seed extract and each dose had 6 replicates. Artesunate (5 mg/kg/d) and pyrimethamine (1.2 mg/kg/d) were used as standard drugs, while distilled water (10 mL/kg/d) served as control. Results: Acute toxicity study produced LD50 of 547.72 mg/kg. The extract demonstrated a dosedependent reduction in parasitaemia in all tests. At the end of 4-day test, suppressive effect of 20.80, 49.00, 75.00 and 88.40% were obtained for doses 54.77, 109.54 and 164.32 mg/kg/d of the seed extract and artesunate, respectively. In the repository test pyrimethamine was more potent (72.26%) than the seed extract (9.47%–51.42%). The extract also exhibited appreciable curative effect. The activity of the seed extract was significant when compared with the control (P < 0.05). Mice treated with the seed extract and drugs survived for longer duration than the control group. Conclusion: The aqueous seed extract of R. communis has antiplasmodial potential and its active principle should be elucidated and further investigated to help in the ongoing fight against malaria.

scholarly journals Acute and Subacute Toxic Aqueous Extract of the Leaves of Petroselinum Crispum Mill. in Male and Female Wistar Rats

2021 ◽  
Vol 17 (40) ◽  
pp. 178
Author(s):  
Kablan Kassi Jean Jacques ◽  
Blahi Adelaïde Nadia, ◽  
Kouakou Koffi Roger ◽  
Diby Yao Seraphin ◽  
Siapo Yao Martin ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Aqueous Extract ◽  
Toxicity Study ◽  
Female Rats ◽  
Petroselinum Crispum ◽  
Male And Female ◽  
Acute Toxicity Study ◽  
Male And Female Rats ◽  
Female Wistar Rats

The present study is part of a vast program of the valorization of the medicinal flora and to help the populations to make a real profit from the use of plants in order to avoid any problem of poisoning. Petroselinum crispum Mill. (Apiaceae) is a plant, whose therapeutic virtues are diverse. The toxicological aspect of the aqueous extract of Petroselinum crispum leaves in male and female rats was investigated. The acute toxicity study with the single dose of 5000 mg/Kg body weight shows that the aqueous extract from the leaves of Petroselinum crispum is not toxic orally. According to Organisation for Economic Cooperation and Development (OECD) Guideline 423, the oral LD50 for this extract is greater than 5000 mg/kg body weight. In addition, the sub-acute toxicity study (OECD 407) showed that the aqueous extract from the leaves of Petroselinum crispum did not show any toxic effects at doses 50,100 and 200 mg/kg body weight and would have an orexigenic effect after 28 days of treatment. The different histological sections showed that the aqueous extract of Petroselinum crispum is not toxic on the vital organs and appears to be hepatoprotective.

scholarly journals Phytochemical and acute toxicity study of aqueous extract of Bambusa vulgaris leaves on Wistar rats

2020 ◽  
Vol 02 (04) ◽  
pp. 107
Author(s):  
Appy Simone Abe ◽  
Kouao Augustin Amonkan ◽  
Kacou Marius Jules Djetouan ◽  
Kesse Philippe N’Da
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Bambusa Vulgaris
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