scholarly journals Study of angiogenesis in cervical intraepithelial lesion and carcinoma using chick chorioallantoic membrane (CAM) assay

2021 ◽  
Vol 5 (5) ◽  
pp. 82-89
Author(s):  
Dr. Princy Dudhwala ◽  
Dr. Rakesh Sharma
Keyword(s):  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Chick Chorioallantoic Membrane ◽  
Cervical Intraepithelial Lesion ◽  
Intraepithelial Lesion

scholarly journals Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

2021 ◽  
Vol 12 ◽  
pp. 204173142110056
Author(s):  
Nupur Kohli ◽  
Vaibhav Sharma ◽  
Alodia Orera ◽  
Prasad Sawadkar ◽  
Nazanin Owji ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Clinical Model ◽  
Chick Chorioallantoic Membrane ◽  
Biomimetic Method ◽  
Alginate Scaffold

Due to the limitations of bone autografts, we aimed to develop new composite biomaterials with pro-angiogenic and osteogenic properties to be used as scaffolds in bone tissue engineering applications. We used a porous, cross-linked and slowly biodegradable fibrin/alginate scaffold originally developed in our laboratory for wound healing, throughout which deposits of calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed the porous nature and confirmed the deposition of CaP precursor phases throughout the scaffolds. MC3T3-E1 cells adhered to the scaffolds, proliferated, migrated and differentiated down the osteogenic pathway during the culture period. Chick chorioallantoic membrane (CAM) assay results showed that the scaffolds were pro-angiogenic and biocompatible. The work presented here gave useful insights into the potential of these pro-angiogenic and osteogenic scaffolds for bone tissue engineering and merits further research in a pre-clinical model prior to its clinical translation.

First experience of chick chorioallantoic membrane (CAM) assay in the clinical work flow with oral squamous cell carcinoma patients

2019 ◽  
Vol 70 (4) ◽  
pp. 487-494 ◽  
Author(s):  
Philipp Kauffmann ◽  
Markus Troeltzsch ◽  
Phillipp Brockmeyer ◽  
Hanibal Bohnenberger ◽  
Paul I. Heidekrüger ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Chorioallantoic Membrane ◽  
Clinical Work ◽  
Work Flow ◽  
Cam Assay ◽  
Chick Chorioallantoic Membrane

scholarly journals In-Situ Forming pH and Thermosensitive Injectable Hydrogels to Stimulate Angiogenesis: Potential Candidates for Fast Bone Regeneration Applications

2020 ◽  
Vol 21 (5) ◽  
pp. 1633 ◽  
Author(s):  
Fatma Z. Kocak ◽  
Abdullah C.S. Talari ◽  
Muhammad Yar ◽  
Ihtesham U. Rehman
Keyword(s):  
Bone Regeneration ◽  
Sol Gel ◽  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Injectable Hydrogels ◽  
Angiogenic Potential ◽  
Homogeneous Solutions ◽  
Chick Chorioallantoic Membrane ◽  
Thermosensitive Hydrogels

Biomaterials that promote angiogenesis are required for repair and regeneration of bone. In-situ formed injectable hydrogels functionalised with bioactive agents, facilitating angiogenesis have high demand for bone regeneration. In this study, pH and thermosensitive hydrogels based on chitosan (CS) and hydroxyapatite (HA) composite materials loaded with heparin (Hep) were investigated for their pro-angiogenic potential. Hydrogel formulations with varying Hep concentrations were prepared by sol–gel technique for these homogeneous solutions were neutralised with sodium bicarbonate (NaHCO3) at 4 °C. Solutions (CS/HA/Hep) constituted hydrogels setting at 37 °C which was initiated from surface in 5–10 minutes. Hydrogels were characterised by performing injectability, gelation, rheology, morphology, chemical and biological analyses. Hydrogel solutions facilitated manual dropwise injection from 21 Gauge which is highly used for orthopaedic and dental administrations, and the maximum injection force measured through 19 G needle (17.191 ± 2.296N) was convenient for manual injections. Angiogenesis tests were performed by an ex-ovo chick chorioallantoic membrane (CAM) assay by applying injectable solutions on CAM, which produced in situ hydrogels. Hydrogels induced microvascularity in CAM assay this was confirmed by histology analyses. Hydrogels with lower concentration of Hep showed more efficiency in pro-angiogenic response. Thereof, novel injectable hydrogels inducing angiogenesis (CS/HA/Hep) are potential candidates for bone regeneration and drug delivery applications.

scholarly journals The In Ovo Chick Chorioallantoic Membrane (CAM) Assay as an Efficient Xenograft Model of Hepatocellular Carcinoma

10.3791/52411 ◽  
2015 ◽  
Author(s):  
Michael Li ◽  
Ravi R. Pathak ◽  
Esther Lopez-Rivera ◽  
Scott L. Friedman ◽  
Julio A. Aguirre-Ghiso ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Chorioallantoic Membrane ◽  
Xenograft Model ◽  
Cam Assay ◽  
In Ovo ◽  
Chick Chorioallantoic Membrane

Evaluation of Electro-Spun Tubular Scaffolds to Create an Anastomosis Using the CAM Assay

2013 ◽  
Author(s):  
Adam Orendain ◽  
Jose Carrasco ◽  
Eniko T. Enikov ◽  
Gholam Peyman
Keyword(s):  
Growth Factor ◽  
Vascular Disease ◽  
Vascular Tissue ◽  
Uv Light ◽  
Chorioallantoic Membrane ◽  
Neovascular Glaucoma ◽  
Collagen Type I ◽  
Type I ◽  
Cam Assay ◽  
Chick Chorioallantoic Membrane

Central retinal vein occlusion (CRVO) is a vascular disease characterized by thrombosis of the retinal veins that can eventually lead to ischemia. Ischemic CRVO can then cause macular degeneration and neovascular glaucoma causing partial to full blindness. In this study, we determined the feasibility of electrospinning tubular scaffolds for treating CRVO and vascular disease. Electrospinning was utilized to produce customizable scaffolds from nano-bers using collagen type I. Scaffolds were treated with glutaraldehyde, glycine, ethanol, UV light, and combinations of the treatments for the purpose cross-linking and to study their angiogenic effects. Structural properties of the scaffolds were analyzed with scanning electron micrsoscopy (SEM). Scaffolds were immobilized with human recombinant vascular endothelial growth factor (rhVEGF165) to investigate the drug-delivering abilities of the electrospun materials and as a method to produce vascularization. The chick chorioallantoic membrane (CAM) assay was used to examine the effects of VEGF immobilizations and to evaluate the feasibility of creating an anastomosis to treat CRVO. Collagen onplants (non-electrospun) and electrospun implants were made on day 10 of embryonic development. Findings show collagen loaded with rhVEGF165 had improved vasculature and pro-angiogenic properties. The present study suggests that collagen can immobilize and release growth factor, be electrospun to mimic the ultrastructure of native blood vessels, and holds promise for vascular tissue engineering.

SERP-1, A NOVEL VIRAL ANTI-INFLAMMATORY SERPIN, IS ANTI-ANGIOGENIC IN CHICK CHORIOALLANTOIC MEMBRANE (CAM) ASSAY

2004 ◽  
Vol 13 (3) ◽  
pp. 75
Author(s):  
David Wong ◽  
Mary Richardson ◽  
Jolanta Stanisz ◽  
Colin Macauley ◽  
Grant McFadden ◽  
...  
Keyword(s):  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Chick Chorioallantoic Membrane ◽  
Anti Inflammatory

scholarly journals Antiangiogenic activity of zinc and zinc-sorafenib combination using the chick chorioallantoic membrane assay: a descriptive study

2017 ◽  
Vol 6 (5) ◽  
pp. 1060
Author(s):  
Manu Kumar ◽  
Girish Gulab Meshram ◽  
Tripti Rastogi ◽  
Sonal Sharma ◽  
Rachna Gupta ◽  
...  
Keyword(s):  
Kinase Inhibitor ◽  
Chorioallantoic Membrane ◽  
Angiogenesis Inhibitor ◽  
Control Group ◽  
Cam Assay ◽  
Dose Ratio ◽  
Antiangiogenic Activity ◽  
Chick Chorioallantoic Membrane ◽  
Branching Points ◽  
Chorioallantoic Membrane Assay

Background: Zinc, a trace element, is known for downregulating several proangiogenic growth factors and cytokines. However, its antiangiogenic activity is not adequately studied. The present study was aimed to evaluate the possible antiangiogenic activity of zinc via the chick chorioallantoic membrane (CAM) assay. Also, the antiangiogenic activity of the combination therapy of zinc with various doses of sorafenib, a tyrosine kinase inhibitor, was evaluated.Methods: A pilot study was initially conducted so as to select suitable doses of zinc and sorafenib. The antiangiogenic activity after combining zinc 2.5 μg/embryo with sorafenib 1, and 2 μg/embryo was also evaluated. The antiangiogenic activity was quantified in terms of total length of blood vessels, number of junctions, number of branching points, and mean length of the blood vessels.Results: Zinc 2.5 μg/embryo showed significant (p <0.05) antiangiogenic activity, as compared to the control group. However, its effect was not comparable to that of sorafenib 2 μg/embryo. The combination of zinc 2.5 μg/embryo with sorafenib 2 μg/embryo did not show an additive/synergistic effect. The combination of zinc 2.5 μg/embryo with sorafenib 1 μg/embryo produced an antiangiogenic activity which was comparable (p >0.05) to that of sorafenib 2 μg/embryo.Conclusions: Zinc caused significant antiangiogenic activity in the CAM assay. The lack of addition/synergism in the zinc-sorafenib combination could have been due to the variability in the dose/ratio selection. Addition of zinc to sorafenib therapy could improve treatment tolerability, reduce cost of therapy, and reduce the emergence of drug resistance. Future mechanistic studies could identify the exact pharmacodynamics of zinc as an angiogenesis inhibitor.

Sign in / Sign up

Export Citation Format

Share Document