scholarly journals Noninvasive in vivo assessment of the skeletal muscle and small intestine serous surface microcirculation in rat: sidestream dark-field (SDF) imaging

2008 ◽  
pp. 365-371
Author(s):  
Z Turek ◽  
V Černý ◽  
R Pařízková
Keyword(s):  
Skeletal Muscle ◽  
Interindividual Variability ◽  
Experimental Studies ◽  
Dark Field ◽  
Sidestream Dark Field ◽  
Small Vessels ◽  
Sdf Imaging ◽  
Orthogonal Polarization Spectral ◽  
Custom Made ◽  
Significant Difference

The pathophysiology of microcirculation is intensively investigated to understand disease development at the microscopic level. Orthogonal polarization spectral (OPS) imaging and its successor sidestream dark-field (SDF) imaging are relatively new noninvasive optical techniques allowing direct visualization of microcirculation in both clinical and experimental studies. The goal of this experimental study was to describe basic microcirculatory parameters of skeletal muscle and ileal serous surface microcirculation in the rat using SDF imaging and to standardize the technical aspects of the protocol. Interindividual variability in functional capillary density (FCD) and small vessels (<25 μm in diameter) proportion was determined in anesthetized rats on the surface of quadriceps femoris (m. rectus femoris and m. vastus medialis) and serous surface of ileum. Special custom made flexible arm was used to fix the SDF probe minimizing the pressure movement artifacts. Clear high contrast images were analyzed off-line. The mean FCD obtained from the surface of skeletal muscle and ileal serous surface was 219 (213-225 cm/cm2) and 290 (282-298 cm/cm2) respectively. There was no statistically significant difference between rats in mean values of FCD obtained from the muscle (P = 0.273) in contrast to ileal serous surface, where such difference was statistically significant (P = 0.036). No statistically significant differences in small vessels percentage was detected on either the muscle surface (P = 0.739) or on ileal serous surface (P = 0.659). Our study has shown that interindividual variability of basic microcirculatory parameters in rat skeletal muscle and ileum is acceptable when using SDF imaging technique according to a highly standardized protocol and with appropriate fixation device. SDF imaging represents promising technology for experimental and clinical studies.

Can sidestream dark field (SDF) imaging identify subtle microvascular changes of the bowel during colorectal surgery?

2018 ◽  
Vol 22 (10) ◽  
pp. 793-800
Author(s):  
A. F. J. de Bruin ◽  
A. L. M. Tavy ◽  
K. van der Sloot ◽  
A. Smits ◽  
C. Ince ◽  
...  
Keyword(s):  
Colorectal Surgery ◽  
Dark Field ◽  
Sidestream Dark Field ◽  
Sdf Imaging ◽  
Microvascular Changes

scholarly journals In Situ Assessment of the Brain Microcirculation in Mechanically-Ventilated Rabbits Using Sidestream Dark-Field (SDF) Imaging

2011 ◽  
pp. 75-81 ◽  
Author(s):  
M. ŠITINA ◽  
Z. TUREK ◽  
R. PAŘÍZKOVÁ ◽  
V. ČERNÝ
Keyword(s):  
Dark Field ◽  
Vessel Density ◽  
Flow Index ◽  
Target Tissue ◽  
Cerebral Microcirculation ◽  
Sidestream Dark Field ◽  
Mechanically Ventilated ◽  
Sdf Imaging ◽  
The Brain

Assessment of the cerebral microcirculation by on-line visualization has been impossible for a long time. Sidestream dark-field (SDF) imaging is a relatively new method allowing direct visualization of cerebral surface layer microcirculation using hand-held probe for direct contact with target tissue. The aim of this study was to elucidate the feasibility of studying the cerebral microcirculation in situ by SDF imaging and to assess the basic cerebral microcirculatory parameters in mechanically ventilated rabbits. Images were obtained using SDF imaging from the surface of the brain via craniotomy. Clear high contrast SDF images were successfully obtained. Total small-vessel density was 14.6±1.8 mm/mm2, total all-vessel density was 17.9±1.7 mm/mm2, DeBacker score was 12.0±1.6 mm-1 and microvascular flow index was 3.0±0.0. This method seems to be applicable in animal studies with possibility to use SDF imaging also intraoperatively, providing unique opportunity to study cerebral microcirculation during various experimental and clinical settings.

scholarly journals Alterations of cerebral microcirculation in peritumoral edema: feasibility of in vivo sidestream dark-field imaging in intracranial meningiomas

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Moncef Berhouma ◽  
Thiebaud Picart ◽  
Chloe Dumot ◽  
Isabelle Pelissou-Guyotat ◽  
David Meyronet ◽  
...  
Keyword(s):  
Mass Effect ◽  
Dark Field ◽  
Sidestream Dark Field ◽  
Reference Pattern ◽  
Who Grade ◽  
Perioperative Morbidity ◽  
Sdf Imaging ◽  
Intracranial Meningiomas ◽  
Normal Reference

Abstract Background Intracranial meningiomas display a variable amount of peritumoral brain edema (PTBE), which can significantly impact perioperative morbidity. The role of microcirculatory disturbances in the pathogenesis of PTBE is still debated. The aim of this study was to microscopically demonstrate and intraoperatively quantify, for the first time, the alterations to microcirculation in PTBE using sidestream dark-field (SDF) imaging. Methods Adult patients with WHO grade I meningiomas were recruited over a 9-month period and divided into 2 groups depending on the absence (NE group) or the presence (E group) of PTBE. In vivo intraoperative microcirculation imaging was performed in the peritumoral area before and after microsurgical resection. Results Six patients were included in the NE group and 6 in the E group. At the baseline in the NE group, there was a minor decrease in microcirculatory parameters compared to normal reference values, which was probably due to the mass effect. In contrast, microcirculatory parameters in the E group were significantly altered, affecting both vessel density and blood flow values, with a drop of approximately 50% of normal values. Surgical resection resulted in a quasi-normalization of microcirculation parameters in the NE group, whereas in the E group, even if all parameters statistically significantly improved, post-resection values remained considerably inferior to those of the normal reference pattern. Conclusion Our study confirmed significant alterations of microcirculatory parameters in PTBE in meningiomas. Further in vivo SDF imaging studies may explore the possible correlation between the severity of these microcirculatory alterations and the postoperative neurological outcome.

scholarly journals Sidestream Dark Field (SDF) imaging of oral microcirculation in the assessment of systemic sclerosis

2019 ◽  
Vol 126 ◽  
pp. 103890
Author(s):  
M. Sha ◽  
M. Griffin ◽  
C.P. Denton ◽  
P.E. Butler
Keyword(s):  
Systemic Sclerosis ◽  
Dark Field ◽  
Sidestream Dark Field ◽  
Sdf Imaging

Abstract 363: Examination of the Effects of Extracorporeal Cardiopulmonary Resuscitation on Sublingual Microcirculation in a Swine Model of Cardiac Arrest

Circulation ◽  
2018 ◽  
Vol 138 (Suppl_2) ◽  
Author(s):  
Brendan M McCracken ◽  
Mohamad H Tiba ◽  
Brandon C Cummings ◽  
Carmen I Colmenero ◽  
Alvaro Rojas-Pena ◽  
...  
Keyword(s):  
Cardiac Arrest ◽  
Real Time ◽  
Tissue Perfusion ◽  
Dark Field ◽  
Swine Model ◽  
Sidestream Dark Field ◽  
Extracorporeal Cardiopulmonary Resuscitation ◽  
Significant Difference ◽  
Highly Correlated ◽  
Time Assessment

Background: Extracorporeal CPR (ECPR) is used to provide circulatory stability for organ perfusion and oxygen delivery (DO 2 ) after refractory cardiac arrest (CA). Hemodynamic measurements during ECPR may not necessarily indicate adequate perfusion at the microcirculatory level where DO 2 , oxygen consumption (VO 2 ), and oxygen exchange (O 2 ER) are most critical. In this study, we used sidestream dark-field imaging to measure total vessel density (TVD) to evaluate sublingual microcirculatory flow in a swine model of refractory CA and ECPR. Hypothesis: We hypothesize that TVD can provide real-time assessment of tissue perfusion during post-cardiac arrest ECPR that correlates with traditional measures of tissue oxygenation. Methods: Swine (8) were anesthetized and instrumented for hemodynamic monitoring. Ventricular fibrillation (VF) was induced and CPR initiated after 8min. CPR was administered using a combination of manual and mechanical chest compressions. During CPR the femoral vessels were instrumented for delivery of veno-arterial ECPR. ECPR was initiated 45min after arrest to simulate refractory CA. Sublingual TVD was measured at baseline and after 15min, 1, 2, and 3 hours of ECPR. Results: A one-way ANOVA showed significant difference between baseline TVD: 12.2(2.3)mm -1 and at 3 hours into ECPR: 6.4(3.0)mm -1 (p=0.005). TVD was highly correlated with circuit flow but not with Mean Arterial Pressure (MAP) (r=0.903, p=0.036; r=0.063, p=0.920). In addition, TVD was highly correlated with DO 2 , and lactate, (r= 0.897, p=0.039; r=-0.883, p=0.047) and moderately with VO 2 , O 2 ER, and ScvO 2 (r= 0.776, -0.370, 0.558) respectively. Conclusion: TVD appears to provide a reliable real-time assessment of tissue perfusion during post-cardiac arrest ECPR. The relationships between TVD and MAP, and TVD and flow also suggest that optimization of flow may be more important than optimizing pressure to achieve adequate tissue perfusion during ECPR.

scholarly journals In situ assessment of the liver microcirculation in mechanically ventilated rats using sidestream dark-field imaging

2009 ◽  
pp. 49-55 ◽  
Author(s):  
V Černý ◽  
Z Turek ◽  
R Pařízková
Keyword(s):  
Animal Studies ◽  
Dark Field ◽  
Target Tissue ◽  
Sidestream Dark Field ◽  
Hepatic Microcirculation ◽  
Midline Laparotomy ◽  
Sdf Imaging ◽  
Liver Microcirculation ◽  
Dark Field Imaging

Assessment of hepatic microcirculation by on-line visualization has been impossible for a long time. Sidestream dark-field (SDF) imaging is a relatively new method allowing direct visualization of both mucosal microcirculation and surface layers microcirculation of solid organs using hand-held probe for direct contact with target tissue. The aim of this study was to evaluate the feasibility of studying the rat hepatic microcirculation in situ by SDF imaging. The liver lobes were left in situ, and images were obtained using SDF imaging on the surface of the liver via upper midline laparotomy. Images were captured intermittently during 10-sec apnoea and recorded. The microvascular parameters were compared with previous validation studies. Clear high contrast SDF images were successfully obtained. Quantitative analysis revealed a mean FSD (functional sinusoidal density) of 402±15 cm/cm2, a sinusoidal diameter of 10.2±0.5 μm and postsinusoidal venular diameter of 33.9±13 μm. SDF imaging is a suitable noninvasive method for accurate quantification of the basic microcirculatory parameters of the liver in situ without a need to exteriorize the liver lobes. This method seems to be applicable in animal studies with possibility to use SDF imaging also intraoperatively, providing unique opportunity to study liver microcirculation during various experimental and clinical settings.

Microvascular perfusion in cardiac arrest: a review of microcirculatory imaging studies

Perfusion ◽  
2017 ◽  
Vol 33 (1) ◽  
pp. 8-15 ◽  
Author(s):  
Petra Krupičková ◽  
Zuzana Mormanová ◽  
Tomáš Bouček ◽  
Tomáš Belza ◽  
Jana Šmalcová ◽  
...  
Keyword(s):  
Cardiac Arrest ◽  
Therapy Monitoring ◽  
Developed Countries ◽  
Dark Field ◽  
Therapeutic Interventions ◽  
Orthogonal Polarization ◽  
Sidestream Dark Field ◽  
Extracorporeal Cardiopulmonary Resuscitation ◽  
Orthogonal Polarization Spectral ◽  
The Impact

Cardiac arrest represents a leading cause of mortality and morbidity in developed countries. Extracorporeal cardiopulmonary resuscitation (ECPR) increases the chances for a beneficial outcome in victims of refractory cardiac arrest. However, ECPR and post-cardiac arrest care are affected by high mortality rates due to multi-organ failure syndrome, which is closely related to microcirculatory disorders. Therefore, microcirculation represents a key target for therapeutic interventions in post-cardiac arrest patients. However, the evaluation of tissue microcirculatory perfusion is still demanding to perform. Novel videomicroscopic technologies (Orthogonal polarization spectral, Sidestream dark field and Incident dark field imaging) might offer a promising way to perform bedside microcirculatory assessment and therapy monitoring. This review aims to summarise the recent body of knowledge on videomicroscopic imaging in a cardiac arrest setting and to discuss the impact of extracorporeal reperfusion and other therapeutic modalities on microcirculation.

Sublingual microcirculation detects impaired perfusion in dehydrated older patients

2020 ◽  
Vol 75 (4) ◽  
pp. 475-487 ◽  
Author(s):  
Raphael Romano Bruno ◽  
Maryna Masyuk ◽  
Johanna M. Muessig ◽  
Stephan Binneboessel ◽  
Michael Bernhard ◽  
...  
Keyword(s):  
Older Patients ◽  
Video Quality ◽  
Clinical Problem ◽  
Dark Field ◽  
Lower Percentage ◽  
Sidestream Dark Field ◽  
Clinical Frailty Scale ◽  
Old Patients ◽  
Small Vessels ◽  
Sublingual Microcirculation

BACKGROUND: Dehydration occurs frequently in older patients and constitutes a significant clinical problem. OBJECTIVE: This proof-of-concept study examines whether 1) sublingual measurement in dehydrated old patients is feasible, 2) frailty and incompliance in old, awake patients affects video-quality, 3) dehydration impacts microcirculation METHODS: This prospective observational study included clinically dehydrated patients aged ≥65 years immediately after admission. Dehydration was assessed clinically. A sidestream dark field camera (SDF) was used for measurement. Video-quality was evaluated with MIQS (microcirculation image quality score). Both AVA 4.3C- and AVA POEM-software analyzed the videos. Seventeen patients ≥65 years not showing dehydration served as control. RESULTS: Thirteen patients (8 female) were included. The average age was 83±8 years. The mini-mental test was 17±15 points, the Clinical Frailty Scale 4±3, the Barthel-Index 59±39. None of these parameters correlated with MIQS (3.4±4.2 SD (“acceptable”)). Dehydrated patients had a slightly impaired microcirculation, with a significantly lower percentage of perfused small vessels compared to control (83.1±7.7% versus 88.0±6.0%, P < 0.05). After rehydration, there was acute improvement in the microcirculation. CONCLUSIONS: Sublingual microcirculatory SDF-measurement is both, safe and valid for dehydrated old patients - regardless of frailty, age or cognitive performance. Dehydration leads to an impaired microcirculation.

scholarly journals Sidestream Dark Field (SDF) imaging: a novel stroboscopic LED ring-based imaging modality for clinical assessment of the microcirculation

2007 ◽  
Vol 15 (23) ◽  
pp. 15101 ◽  
Author(s):  
P. T. Goedhart ◽  
M. Khalilzada ◽  
R. Bezemer ◽  
J. Merza ◽  
C. Ince
Keyword(s):  
Clinical Assessment ◽  
Imaging Modality ◽  
Dark Field ◽  
Sidestream Dark Field ◽  
Sdf Imaging
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