Early and late effects of hyperbaric oxygen treatment on oxidative stress parameters in diabetic patients

Exposure to hyperbaric oxygen leads to increased amount of reactive oxygen species (ROS) that are derived from various sources. After the discovery that ROS can function as signaling molecules, the idea of ROS being hazardous to biological tissues has been challenged. The aim of this study was to examine the changes in oxidative stress parameters in diabetics undergoing hyperbaric oxygen therapy (HBOT) due to foot ulcers. Twenty patients, who received HBOT for diabetic foot ulcers, were included in the study. Blood samples were taken before HBOT and 30 min after exit from the chamber, on the day of the first and the 15th HBOT sessions. They were used for the determinations of malondialdehyde (MDA), 8-isoprostane and advanced oxidation protein products (AOPPs). 8-Isoprostane and AOPP levels were not altered significantly after the first HBOT session, while both were increased on the fifteenth day (p<0.05). MDA was significantly increased only after the first HBOT session, and remained unchanged on the fifteenth day (within-day variations). Plasma AOPP levels were lowered significantly after fifteen consecutive HBOT sessions (between-day variations). Decreased AOPP levels suggest that increased oxygenation of tissues due to HBO therapy may activate some endogenous factors that prevent hazardous effects of the disease itself.

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Clinical Significance of ROMs, OXY, SHp and HMGB-1 in Canine Leishmaniosis

Animals ◽

10.3390/ani11030754 ◽

2021 ◽

Vol 11 (3) ◽

pp. 754

Author(s):

Michela Pugliese ◽

Alessandra Sfacteria ◽

Gaetano Oliva ◽

Annastella Falcone ◽

Manuela Gizzarelli ◽

...

Keyword(s):

Oxidative Stress ◽

Lymph Nodes ◽

Clinical Signs ◽

Biological Tissues ◽

Stress Index ◽

Control Group ◽

Canine Leishmaniosis ◽

Oxidative Stress Parameters ◽

Stress Parameters

This study aimed to investigate the role of oxidative stress parameters (ROMs, OXY, SHp), the Oxidative Stress index (OSi), and High Mobility Group Box-1 protein (HMGB-1) in canine leishmaniosis (CanL). For this study, thirty dogs, naturally infected with Leishmania spp. (Leishmania Group, LEISH) and ten healthy adult dogs (control group, CTR) were included. The diagnosis of CanL was performed by a cytological examination of lymph nodes, real time polymerase chain reaction on biological tissues (lymph nodes and whole blood), and an immunofluorescence antibody test (IFAT) for the detection of anti-Leishmania antibodies associated with clinical signs such as dermatitis, lymphadenopathy, onychogryphosis, weight loss, cachexia, lameness, conjunctivitis, epistaxis, and hepatosplenomegaly. The HMGB-1 and oxidative stress parameters of the LEISH Group were compared with the values recorded in the CTR group (Mann Whitney Test, p < 0.05). Spearman rank correlation was applied to evaluate the correlation between the HMGB-1, oxidative stress biomarkers, hematological and biochemical parameters in the LEISH Group. Results showed statistically significant higher values of SHp in the LEISH Group. Specific correlation between the ROMs and the number of red blood cells, and between HGMB-1 and SHp were recorded. These preliminary data may suggest the potential role of oxidative stress in the pathogenesis of CanL. Further studies are undoubtedly required to evaluate the direct correlation between inflammation parameters with the different stages of CanL. Similarly, further research should investigate the role of ROMs in the onset of anemia.

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Hyperbaric oxygen treatment reverses radiation induced pro-fibrotic and oxidative stress responses in a rat model

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2016.12.036 ◽

2017 ◽

Vol 103 ◽

pp. 248-255 ◽

Cited By ~ 12

Author(s):

N. Oscarsson ◽

L. Ny ◽

J. Mölne ◽

F. Lind ◽

S.-E. Ricksten ◽

...

Keyword(s):

Oxidative Stress ◽

Hyperbaric Oxygen ◽

Rat Model ◽

Stress Responses ◽

Hyperbaric Oxygen Treatment ◽

Oxygen Treatment ◽

Radiation Induced ◽

Oxidative Stress Responses ◽

And Oxidative Stress

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Effect of atorvastatin on oxidative stress parameters and lipid profile in type 2 diabetic patients

IIUM Medical Journal Malaysia ◽

10.31436/imjm.v7i2.783 ◽

2020 ◽

Vol 7 (2) ◽

Author(s):

Najah RH ◽

Mohammad AAH ◽

Ammar RMR

Keyword(s):

Oxidative Stress ◽

Lipid Profile ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Diabetic Patients ◽

Type 2 Diabetic Patients ◽

Oxidative Stress Parameters ◽

Type 2 Diabetic ◽

Stress Parameters

Introduction: Evidence has long existed regarding the relationship between oxidative stress and diabetes. The present study was conducted to assess the effect of atorvastatin on selected oxidative stress parameters in the form of reduced glutathione (GSH), lipid peroxidation byproduct malondialdehyde (MDA) levels, glutathione –S- transferase (GST) activity and catalase (CAT) activity) and its effect on lipid profile (total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) in dyslipidaemic type 2 diabetic patients . Materials and Methods: Fifty nine dyslipidaemic type 2 diabetic patients were included in this study. Full history was taken and general examination of patients was performed. Patients studied were taking glibenclamide (an oral hypoglycaemic drug) during the study as a treatment for their disease. These patients were followed up for 60 days and divided randomly into 2 groups. Group I (n = 31): no drug was given and served as dyslipidaemic diabetic control. Group II (n = 28): received atorvastatin tablets 20 mg once daily at night. Of the 59 Fifty patients, 46 completed the study while 13 patients withdrew. This is due to non compliance of the patients. Blood samples were drawn from the patients at the beginning and after 60 days of follow up between 8:30 & 10:30 am after at least 12-14 hours fast. Fasting blood glucose, lipid profile, selected oxidative stress parameters (GSH, MDA levels, GST and CAT activities) were measured. Renal and hepatic functions were also assessed. Results: This study revealed that: atorvastatin treatment increased serum GSH; reduced MDA levels significantly while did not significantly affect CAT and GST activity. In atorvastatin treatment, TC, TG, LDL and VLDL decreased significantly while HDL increased significantly. Conclusion: There was insignificant correlations between atorvastatin induced changes in the oxidation markers and the observed changes of the lipid profile.

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Hyperbaric Oxygen Treatment: Effects on Mitochondrial Function and Oxidative Stress

Biomolecules ◽

10.3390/biom11121827 ◽

2021 ◽

Vol 11 (12) ◽

pp. 1827

Author(s):

Nofar Schottlender ◽

Irit Gottfried ◽

Uri Ashery

Keyword(s):

Oxidative Stress ◽

Hyperbaric Oxygen ◽

Mitochondrial Function ◽

Defense Mechanisms ◽

Term Treatment ◽

Mitochondrial Activity ◽

Hyperbaric Oxygen Treatment ◽

Oxygen Treatment ◽

Long Term Treatment ◽

And Oxidative Stress

Hyperbaric oxygen treatment (HBOT)—the administration of 100% oxygen at atmospheric pressure (ATA) greater than 1 ATA—increases the proportion of dissolved oxygen in the blood five- to twenty-fold. This increase in accessible oxygen places the mitochondrion—the organelle that consumes most of the oxygen that we breathe—at the epicenter of HBOT’s effects. As the mitochondrion is also a major site for the production of reactive oxygen species (ROS), it is possible that HBOT will increase also oxidative stress. Depending on the conditions of the HBO treatment (duration, pressure, umber of treatments), short-term treatments have been shown to have deleterious effects on both mitochondrial activity and production of ROS. Long-term treatment, on the other hand, improves mitochondrial activity and leads to a decrease in ROS levels, partially due to the effects of HBOT, which increases antioxidant defense mechanisms. Many diseases and conditions are characterized by mitochondrial dysfunction and imbalance between ROS and antioxidant scavengers, suggesting potential therapeutic intervention for HBOT. In the present review, we will present current views on the effects of HBOT on mitochondrial function and oxidative stress, the interplay between them and the implications for several diseases.

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Assessment of Glycemic Control, Renal Function, and Oxidative Stress Parameters in Type 2 Diabetes MellitusPatients

Iraqi Journal of Science ◽

10.24996/ijs.2021.62.12.4 ◽

2021 ◽

pp. 4628-4638

Author(s):

Hawraa Sabah Al-Musawi ◽

MakarimQassim Al-Lami ◽

Ali H. Al-Saadi

Keyword(s):

Oxidative Stress ◽

Renal Function ◽

Blood Glucose ◽

Glycemic Control ◽

Inverse Correlation ◽

Diabetic Patients ◽

Positive Correlation ◽

Oxidative Stress Parameters ◽

Chronic Hyperglycemia ◽

Stress Parameters

Diabetes mellitus (T2DM) is a multifactorial syndrome that israpidly rising in all the continents ofthe globe, causing elevated blood sugar levels in affected people. A sample of 81 Iraqi T2DM patients was investigated based on several parameters. Glycemic control parameters includedlevels of fasting blood glucose (FBG),glycated hemoglobin (HbA1C), and insulin, along with insulin resistance (IR) and insulin sensitivity (IS). Renal function tests includedmeasuring the blood levels of urea and creatinine. Oxidative stress parameters included total antioxidant capacity (TAC) and thelevel of reactive oxygen species (ROS). The results of the presentstudy showed a highly significant (P˂0.01) increase in FBG, HbA1c, insulin and IR levels in T2DM patients as compared to control.Insulin sensitivity showed a highly significant (p˂0.01) decrease in patients compared with control.Urea and creatinine levelsincreased in T2DM patients, but the differences were insignificant. TAC levelsignificantly (P<0.05) increased in patients compared with control. Also, the levels of ROSrevealed a highly significant (P<0.01) increasein T2DM patients compared with the control. Correlation analysis showedthat FBG has a highly significant (P<0.01) positive correlation with IR, urea, creatinine and ROS, as well as a significant (P<0.05) positive correlation with TAC. However, FBG shows a highlysignificant (P< 0.01) inverse correlation with IS. The levels of HbA1C show a significant (P<0.05) positive correlation with IR, creatinine, and TAC, whereas ithas a highly significant (P<0.01) positive relation with ROS. However, HbA1C level has a highly significant (P<0.01) inverse relation with IS. Insulin has highly significant (P<0.01) positive and negative associations with IR and IS, respectively.IR showshighlya significant (P<0.01) inverse correlation with IS, significant (P<0.05) positive correlation with creatinine, and highly significant (P<0.01) positive correlation with ROS. IS has a significant(P< 0.05) inverse correlation with urea. Urea shows a highly significant (P<0.01) positive correlation with creatinine. TAC has a significant (P<0.05) inverse correlation with ROS. Conclusion: diabetic patients revealed poor glycemic control. Fluctuating blood glucose concentrations may contribute significantly to oxidative stress, probablyeven more than chronic hyperglycemia. The observed significant positive correlation between FBG and the other tested parameters revealed that hyperglycemia is an obvious independent risk factor for T2DM progression.

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Advantages and Disadvantages of Hyperbaric Oxygen Treatment in Mice with Obesity Hyperlipidemia and Steatohepatitis

The Scientific World JOURNAL ◽

10.1100/2011/380236 ◽

2011 ◽

Vol 11 ◽

pp. 2124-2135 ◽

Cited By ~ 11

Author(s):

Koichi Tsuneyama ◽

Yen-Chen Chen ◽

Makoto Fujimoto ◽

Yoshiyuki Sasaki ◽

Wataru Suzuki ◽

...

Keyword(s):

Oxidative Stress ◽

Hyperbaric Oxygen ◽

Lipid Level ◽

The Body ◽

Cellular Damage ◽

Control Group ◽

Second Phase ◽

Hyperbaric Oxygen Treatment ◽

Advantages And Disadvantages ◽

Oxygen Treatment

The effect of hyperbaric oxygen treatment (HBOT) was examined using MSG mice, which are an animal model of obesity, hyperlipidemia, diabetes, and nonalcoholic fatty liver disease. Nineteen MSG male mice were divided into HBOT treated and control groups at 12 weeks of ages. The HBOT group was treated with hyperbaric oxygen from 12 to 14 weeks (first phase) and then from 16 to 18 weeks (second phase). Interestingly, the body weight of the HBOT group was significantly lower (P<0.01) than that of the control group. In contrast, the serum lipid level did not show significant changes between the two groups. As for the effects of increasing oxidative stress, the liver histology of the HBOT group showed severer cellular damage and aberrant TNF-α expression. HBOT has the advantage of improving obesity in patients with metabolic syndrome, but the fault of causing organ damage by increasing oxidative stress.

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