scholarly journals Protein Interactions With Quaternized Chitosan/Heparin Multilayers

2016 ◽  
pp. S253-S261 ◽  
Author(s):  
M. KUMOREK ◽  
D. KUBIES ◽  
T. RIEDEL
Keyword(s):  
Protein Interactions ◽  
Electrostatic Interactions ◽  
Multilayer Film ◽  
Adsorption Process ◽  
Surface Coatings ◽  
Polyelectrolyte Multilayer ◽  
Quaternized Chitosan ◽  
Solution Interface ◽  
Polyelectrolyte Multilayer Film ◽  
Polysaccharide Film

Understanding the behavior of single proteins at the polyelectrolyte multilayer film/solution interface is of prime importance for the designing of bio-functionalized surface coatings. In the present paper, we study the adsorption of the model proteins, albumin and lysozyme, as well as basic fibroblast growth factor (FGF-2) on a polysaccharide multilayer film composed of quaternized chitosan and heparin. Several analytical methods were used to describe the formation of the polysaccharide film and its interactions with the proteins. Both albumin and lysozyme adsorbed on quaternized chitosan/heparin films, however this process strongly depended on the terminating polysaccharide. Protein adsorption was driven mainly by electrostatic interactions between protein and the terminal layer of the film. The effective binding of FGF-2 by the heparin-terminated film suggested that other interactions could also contribute to the adsorption process. We believe that this FGF-2-presenting polysaccharide film may serve as a biofunctional surface coating for biologically-related applications.

Kinetics of Salt-Induced Annealing of a Polyelectrolyte Multilayer Film Morphology

Langmuir ◽  
2003 ◽  
Vol 19 (9) ◽  
pp. 3947-3952 ◽  
Author(s):  
Richard A. McAloney ◽  
Vyacheslav Dudnik ◽  
M. Cynthia Goh
Keyword(s):  
Multilayer Film ◽  
Film Morphology ◽  
Polyelectrolyte Multilayer ◽  
Kinetics Of ◽  
Polyelectrolyte Multilayer Film

Combinatorial Polyelectrolyte Multilayer Film Fabrication

2009 ◽  
Vol 9 (11) ◽  
pp. 6421-6426 ◽  
Author(s):  
Matthew M. Coulter ◽  
David S. Dos Santos ◽  
Richard W. Loo ◽  
M. Cynthia Goh
Keyword(s):  
Multilayer Film ◽  
Polyelectrolyte Multilayer ◽  
Film Fabrication ◽  
Polyelectrolyte Multilayer Film

Polyelectrolyte Multilayer Film Coating and Stability at the Surfaces of Oral Prosthesis Base Polymers: an in vitro and in vivo Study

2006 ◽  
Vol 85 (1) ◽  
pp. 44-48 ◽  
Author(s):  
O. Etienne ◽  
C. Picart ◽  
C. Taddei ◽  
P. Keller ◽  
E. Hubsch ◽  
...  
Keyword(s):  
Multilayer Film ◽  
Film Coating ◽  
Human Saliva ◽  
Layer By Layer ◽  
Polyelectrolyte Multilayer ◽  
Layer Technique ◽  
Polyelectrolyte Multilayer Film ◽  
Layer By Layer Technique

A new type of coating involving a layer-by-layer technique has been recently reported. This coating is composed of a polyelectrolyte multilayer film that confers specific properties on surfaces to which it is applied. Here, we studied the applicability of such a technique to the coating of oral prostheses, by first testing the construction of polyelectrolyte multilayer films on several polymers used in oral prosthesis bases, and, subsequently, by studying the stability of these coatings in vitro, in human saliva, and in vivo in a rat model. We demonstrated that the multilayered films are able to coat the surfaces of all tested polymers completely, thus increasing their wettability. We also showed that saliva does not degrade the film after 7 days in vitro and after 4 days in vivo. Taken together, our results establish that the layer-by-layer technique is suitable for the coating of oral devices.

Using a biocompatible diazidecrosslinker to fabricate a robust polyelectrolyte multilayer film with enhanced effects on cell proliferation

2017 ◽  
Vol 5 (2) ◽  
pp. 375-381 ◽  
Author(s):  
Yue Wang ◽  
Wenjun You ◽  
Yifan Song ◽  
Xiangxin Li ◽  
Dengli Qiu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Multilayer Film ◽  
Polyelectrolyte Multilayer ◽  
Positive Effects ◽  
Polyelectrolyte Multilayer Film

We have proposed a biocompatible diazidecrosslinker with positive effects on cell proliferation based on a facile and versatile crosslinking strategy.

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