EFFECTS OF PRESERVATIVE TREATMENTS ON THE MICROBIOLOGICAL AND STORAGE QUALITY OF TIGER NUT MILK DRINK
Tiger nut milk drink is a milk-like aqueous extract from tiger nut, which is highly nutritious and is consumed in most parts of the world especially in the Northern part of Nigeria. However, its availability is limited due to its short shelf life. The aim of this present study was to determine the effects of preservatives treatments on the microbiological and storage quality of tiger nut milk drink. Fresh tiger nut milk drink was produced according to the standard procedure with portable water and also with sterile distilled water. The milk was divided into 16 aliquots, kept in a sterile container for analysis of the different treatments, and preservatives were added according to the experimental design. The color, odor and consistency of the milk were observed daily for a period of seven days. The total bacteria count (TBC), total coliform count (TCC), total Staphylococcal count (TSC) and total fungi count (TFC) (yeast and mold) were evaluated every two days to monitor the microbial stability. Fresh tiger nut milk without any treatment spoiled within few hours after production, while the aliquot supplemented with all the preservatives; pasteurized and then stored under refrigerated temperature for up to seven days. The range of total bacterial count was from 8.00×104 -1.20×107 CFU/ml. The most prevalent bacterial isolates were; Escherichia coli (17.79%), Staphylococcus aureus (10.53%) and Micrococcus spp. (9.78%), whereas the dominant fungi were Candida tropicalis (16.27%), Saccharomyces spp. (11.63%) and Aspergillus spp. (11.63%). This study showed that tiger nut milk drink has a rich microbial content which affects its shelf life and the addition of natural (spices) and chemical preservatives is important to stabilize the drink; thus there is an urgent need to standardize the use of preservatives.