scholarly journals Analisis Kandungan Fitokimia dan Aktivitas Antioksidan Mikroalga Chlorella sp. Berdasarkan Variasi Waktu Pencahayaan

2021 ◽  
Vol 8 (2) ◽  
pp. 139-146
Author(s):  
Hartini Hartini ◽  
Karolina Rosmiati ◽  
Agnes Farianti Rezeki Sihombing
Keyword(s):  

Mikroalga mengandung metabolit sekunder yang potensial untuk dikembangkan. Metabolit sekunder digunakan untuk pertahanan kimia terhadap predator di lingkungan air. Salah satu jenis mikroalga yang dapat dikembangkan metabolit sekundernya adalah Chlorella sp. Kondisi lingkungan saat kultivasi Chlorella sp. mempengaruhi jumlah sel, kandungan metabolit sekunder dan kestabilan senyawa antioksidan mikroalga. Faktor lingkungan yang menjadi fokus penelitian adalah faktor cahaya. Penelitian ini bertujuan untuk menganalisis kandungan fitokimia dan antioksidan biomassa mikroalga Chlorella sp. dengan variasi waktu pencahayaan. Variasi pemberian cahaya pada proses kultivasi menggunakan periode terang : gelap yang terbagi menjadi 2 kelompok yaitu 12:12 (terang:gelap) jam dan 18:6 (terang:gelap) jam. Metode yang digunakan untuk analisis kandungan fitokimia menggunakan metode Harborne dan analisis aktivitas antioksidan menggunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl). Hasil penelitian menunjukkan bahwa ekstrak mikroalga Chlorella sp. dengan variasi waktu pencahayaan (terang:gelap) 12:12 jam mengandung alkaloid, flavonoid, saponin, steroid, kuinon dan terpenoid. Sedangkan, ekstrak mikroalga Chlorella sp. dengan variasi waktu pencahayaan (terang:gelap) 18:6 jam mengandung alkaloid, flavonoid, saponin dan terpenoid. Hasil aktivitas antioksidan ekstrak mikroalga Chlorella sp. dengan variasi waktu pencahayaan (terang:gelap) 12:12 jam IC50 sebesar 40,421 dan ekstrak Chlorella sp. dengan variasi waktu pencahayaan (terang:gelap) 18:6 jam memiliki IC50 sebesar 8,992. Berdasarkan penelitian diperoleh bahwa ekstrak mikroalga yang diberi waktu pencahayaan tinggi menghasilkan aktivitas antioksidan yang lebih kuat dibandingkan pencahayaan rendah.

2018 ◽  
Vol 159 (18) ◽  
pp. 703-708
Author(s):  
Gábor Vasas

Abstract: More than 90% of herbal products and herbal medicines have been derived from higher plants recently, but due to independent circumstances, several photosynthetic microalgal species are in focus in this point of view. In the last 50 years, many carbohydrate-, peptide-, terpenoid-, alkaloid- and phenol-type components were described from algae because of the developing structural determination and analytical methods, algae mass production and also artificial algae technologies. At the same time, based partly on traditional causes and partly on the clinical and preclinical data of today, some dried products of algae are directly used as food supplements. Hereinafter, the historical background, economic significance and metabolic background of the mostly used microalgal species will be reviewed. The diverse metabolite production of these organisms will be demonstrated by some molecules with special bioactivity. Several preclinical and clinical studies will be described relating to the microalgal species Spirulina sp., Chlorella sp., Haematococcus sp. and Dunaliella sp. Orv Hetil. 2018; 159(18): 703–708.


2016 ◽  
Vol 04 ◽  
pp. 1 ◽  
Author(s):  
Phong, H.Q. ◽  
Dat, N.V. ◽  
Huong, H.L. ◽  
Au, T.D.

Chemistry ◽  
2021 ◽  
Vol 3 (3) ◽  
pp. 821-830
Author(s):  
Davide De Simeis ◽  
Stefano Serra ◽  
Alessandro Di Fonzo ◽  
Francesco Secundo

Natural flavor and fragrance market size is expected to grow steadily due to the rising consumer demand of natural ingredients. This market request is guided by the general opinion that the production of natural compounds leads to a reduction of pollution, with inherent advantages for the environment and people’s health. The biotransformation reactions have gained high relevance in the production of natural products. In this context, few pieces of research have described the role of microalgae in the oxidation of terpenoids. In this present study, we questioned the role of microalgal based oxidation in the synthesis of high-value flavors and fragrances. This study investigated the role of three different microalgae strains, Chlorella sp. (211.8b and 211.8p) and Chlorococcum sp. (JB3), in the oxidation of different terpenoid substrates: α-ionone, β-ionone, theaspirane and valencene. Unfortunately, the experimental data showed that the microalgal strains used are not responsible for the substrate oxidation. In fact, our experiments demonstrate that the transformation of the four starting compounds is a photochemical reaction that involves the oxygen as oxidant. Even though these findings cast a shadow on the use of these microorganisms for an industrial purpose, they open a new possible strategy to easily obtain nootkatone in a natural way by just using an aqueous medium, oxygen and light.


Fuel ◽  
2019 ◽  
Vol 257 ◽  
pp. 116026 ◽  
Author(s):  
Quazi Mahzabin Rahman ◽  
Bo Zhang ◽  
Lijun Wang ◽  
Abolghasem Shahbazi

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2410
Author(s):  
Jean Claude Nzayisenga ◽  
Anita Sellstedt

There are numerous strains of Chlorella with a corresponding variety of metabolic pathways. A strain we previously isolated from wastewater in northern Sweden can grow heterotrophically as well as autotrophically in light and has higher lipid contents under heterotrophic growth conditions. The aims of the present study were to characterize metabolic changes associated with the higher lipid contents in order to enhance our understanding of lipid production in microalgae and potentially identify new compounds with utility in sustainable development. Inter alia, the amino acids glutamine and lysine were 7-fold more abundant under heterotrophic conditions, the key metabolic intermediate alpha-ketoglutarate was more abundant under heterotrophic conditions with glucose, and maltose was more abundant under heterotrophic conditions with glycerol than under autotrophic conditions. The metabolite 3-hydroxy-butyric acid, the direct precursor of the biodegradable plastic PHB (poly-3-hydroxy-butyric acid), was also more abundant under heterotrophic conditions. Our metabolomic analysis has provided new insights into the alga’s lipid production pathways and identified metabolites with potential use in sustainable development, such as the production of renewable, biodegradable plastics, cosmetics, and nutraceuticals, with reduced pollution and improvements in both ecological and human health.


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