scholarly journals SINGLE SPORE ISOLATION AND MOLECULAR CHARACTERIZATION OF TRICHODERMA SPECIES FROM AGRICULTURAL FIELDS OF ZHEJIANG PROVINCE, CHINA

2018 ◽  
Vol 30 (2) ◽  
pp. 115
Author(s):  
Baharullah Khattak ◽  
Hong K. Wang ◽  
Fucheng Lin ◽  
Saif Ullah ◽  
Qaiser Jamal ◽  
...  
Keyword(s):  
Plant Pathogens ◽  
Agricultural Soils ◽  
Biological Control Agent ◽  
Control Agent ◽  
Soil Samples ◽  
Zhejiang Province ◽  
Single Spore ◽  
Trichoderma Spp ◽  
Trichoderma Species ◽  
Trichoderma Sp

It is well known that Trichoderma spp. can be used as a biological control agent against variety of plant pathogens. In this study, Trichoderma species were isolated from rhizosphere samples of various sites in Zhejiang province, China. A total of 41 soil samples were processed for the isolation of single cell culture of Trichoderma sp. Out of which, 32 species of the genus Trichoderma, were isolated and characterized by using single spore isolation method. The isolates were subjected to sequencing, for precise identification up to species level. Trichoderma harzianum was isolated from maximum (09) number of soil samples, followed by T. velutinum, isolated from 3 soil samples. The other isolated species of the genus Trichoderma were; lixii, atroviride, koningii, longibrachatum, rossicum, gamsii, koningiopsis, tomentosum, afroharzianum, viride, citroviride, aureoviride, simmonsii and hamatum. It was concluded that Trichoderma sp. were abundantly found in the agricultural soils of Zhejiang province, China.

scholarly journals Exploration and Identification Trichoderma spp. as a Biological Control Agents to Plant Pathogens and Starter Making Biological Fertilizers

2020 ◽  
Vol 13 (1) ◽  
pp. 222-226
Author(s):  
Henny V.G. Makal ◽  
Max M. Ratulangi ◽  
Denny S. Sualang
Keyword(s):  
Biological Control ◽  
Population Density ◽  
Plant Pathogens ◽  
Soil Samples ◽  
Dilution Method ◽  
Biological Control Agents ◽  
Trichoderma Spp ◽  
Trichoderma Species ◽  
Trichoderma Sp ◽  
Biological Fertilizer

The objectives of this study are: (1) to inventory Trichoderma spp. in North Minahasa District, South Minahasa District, and Tomohon City-Minahasa District, (2) inventory of Trichoderma spp. in the rhizosphere of cultivated and fallow gardens, and (3) calculate the population density of Trichoderma spp. all soil samples. The scope of this study is the biological control of plant pathogens, induce plant resistance, and biological fertilizer production. Trichoderma isolation spp. has been carried out by dilution method and cultured on PDA + antibiotics. Population density of Trichoderma spp. calculated using the plate calculation method. Identification of this species function based on the color and patterns of sporulation in the colony; hyphae and clamydospores; conidiophores; and phialides and phialospores. Trichoderma species found in North Minahasa District were T. harzianum, T. koningii, and T. viride; in South Minahasa District, T. koningii and T. viride; and in Tomohon City-Minahasa District, T. koningii and T. viride. In fallow gardens were T. harzianum, T. koningii, and T. viride, and in cultivated gardens were T. koningii and T. viride. Population densities of Trichoderma sp. in South Minahasa District, North Minahasa District, and Tomohon City-Minahasa District, respectively 1,363.64, 466.67, and 26.67 CFU / g soil.

scholarly journals Effect of Trichoderma Spp. as a Bio-control Agent on Cereal Crop Plants

2020 ◽  
Vol 3 (5) ◽  
pp. 65-68
Author(s):  
Shankar Gopinath Yadav
Keyword(s):  
Seed Germination ◽  
Culture Filtrate ◽  
Plant Pathogens ◽  
Germination Percentage ◽  
Control Agent ◽  
Trichoderma Virens ◽  
Cereal Crop ◽  
Trichoderma Spp ◽  
Trichoderma Species ◽  
Phytotoxic Effect

Trichoderma is a Deuteromycetous fungus with hyphae long, creeping, conidiophores erect, philiadic, having conidial heads. Trichoderma species produces a chemical, trichodermin; it is the sesquiteroenoid acting as a bio-control agent on the principal of myco-parasitism, antibiosis and competition to control soil borne plant pathogens. The phytotoxic effect was screened by treating cereal crop seeds with culture filtrate of Trichoderma species, the effect of culture filtrate was measured in terms of seed germination percentage and results were compared with control one. The obtained results shows percentage of germination in treated seeds was more than the control. The culture filtrate of Trichoderma harzianum showed superior effects (95-97%) than that of Trichoderma viridae (90-95%) and Trichoderma virens (86-92%) on the seed germination of selected cereal crop seeds.

Field evaluation of Trichoderma spp. as a biological control agent to prevent wood decay on Benin mahogany ( Khaya grandifoliola ) and rain tree ( Samanea saman ) in Singapore

2017 ◽  
Vol 114 ◽  
pp. 114-124 ◽  
Author(s):  
Daniel C. Burcham ◽  
Nelson V. Abarrientos ◽  
Jia Yih Wong ◽  
Mohamed Ismail Mohamed Ali ◽  
Yok King Fong ◽  
...  
Keyword(s):  
Biological Control ◽  
Biological Control Agent ◽  
Wood Decay ◽  
Field Evaluation ◽  
Control Agent ◽  
Trichoderma Spp ◽  
Samanea Saman

Biotechnology and Environmental applications of Trichoderma spp.

2021 ◽  
pp. 149-157
Author(s):  
Waill A. Elkhateeb ◽  
Marwa O. Elnahas ◽  
Ghoson M. Daba ◽  
Abdel-Nasser A. Zohri
Keyword(s):  
Biological Control ◽  
Agricultural Soils ◽  
Hydrolytic Enzymes ◽  
Biological Control Agents ◽  
Growth Promoters ◽  
Trichoderma Spp ◽  
Trichoderma Species ◽  
Plant Growth Promoters ◽  
Different Strains ◽  
Soil Borne Fungi

The genus Trichoderma is multicultural soil-borne fungi found in different ecosystems. They are highly successful colonizers of their habitats. Genus Trichoderma is capable of dealing with various environments such as compost, agricultural soils, rhizosphere, and waste material. Therefore, different strains of Trichoderma have been applied in agriculture, bioremediation, waste management, and biotechnology. Many Trichoderma species act as biological control agents and plant growth promoters. Additionally, the genus Trichoderma is a new fungal source for the production of cyclosporin A as well as various hydrolytic enzymes with industrial importance.

scholarly journals In-vitro Evaluation of Bio-control agents against Soil Borne Plant Pathogens

2019 ◽  
Vol 5 ◽  
pp. 68-72
Author(s):  
Shrinkhala Manandhar ◽  
Bimala Pant ◽  
Chetana Manandhar ◽  
Suraj Baidya
Keyword(s):  
Plant Pathogens ◽  
Field Experiments ◽  
Colony Growth ◽  
Dual Culture ◽  
Vitro Assay ◽  
Trichoderma Species ◽  
Soil Borne Pathogens ◽  
Trichoderma Sp ◽  
Trichoderma Isolates

Biocontrol is an important aspect of disease management for plant pathogens, especially for the soil borne fungi. Trichoderma species is the most exploited biocontrol agent in recent years. The soil specific nature of Trichoderma species is a well-known fact and hence native Trichoderma isolates should be more emphasized for control of plant pathogens. Fifty soil samples from rhizosphere of various agricultural crops were collected for isolation of Trichoderma sp. Ten isolates of Trichoderma were tested in dual culture with soil borne pathogens Fusarium solani, Rhizoctonia solani and Sclerotinia sclerotiorum in an in vitro assay. All of the test isolates were found to be significant in terms of mycelial inhibition growth as compared to control. However, varying degrees of antagonism by different Trichoderma isolates were observed for above mentioned soil borne pathogens. The isolate (T363) was found to exhibit more than 80% inhibition of S. sclerotiorum while the isolate T357 was found to control F. solani by more than 80%.  For the control of R. solani, six of the tested Trichoderma isolates showed more than 80% inhibition of its radial colony growth. The Trichoderma isolates seen effective in this study need to be tested in pot and field experiments for exploiting the use and benefits of biocontrol.

A pipeline for the genetic improvement of a biological control agent enhances its potential for controlling soil-borne plant pathogens

2021 ◽  
Vol 152 ◽  
pp. 104460
Author(s):  
Marcio Vinicius de Carvalho Barros Côrtes ◽  
Maythsulene Inacio de Sousa Oliveira ◽  
Jackeline Rossetti Mateus ◽  
Lucy Seldin ◽  
Valacia Lemes Silva-Lobo ◽  
...  
Keyword(s):  
Biological Control ◽  
Genetic Improvement ◽  
Plant Pathogens ◽  
Biological Control Agent ◽  
Control Agent

Development of a strain-specific SCAR marker for the detection of Trichoderma atroviride 11, a biological control agent against soilborne fungal plant pathogens

2001 ◽  
Vol 38 (6) ◽  
pp. 343-350 ◽  
Author(s):  
M. Rosa Hermosa ◽  
Isabel Grondona ◽  
José María Díaz-Mínguez ◽  
Enrique A. Iturriaga ◽  
Enrique Monte
Keyword(s):  
Biological Control ◽  
Plant Pathogens ◽  
Scar Marker ◽  
Biological Control Agent ◽  
Control Agent ◽  
Trichoderma Atroviride ◽  
Fungal Plant Pathogens

scholarly journals EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG

2017 ◽  
Vol 16 (2) ◽  
pp. 115
Author(s):  
Johanna Taribuka ◽  
Christanti Sumardiyono ◽  
Siti Muslimah Widyastuti ◽  
Arif Wibowo
Keyword(s):  
Biological Control ◽  
Phylogenetic Analysis ◽  
Endophytic Fungi ◽  
Trichoderma Harzianum ◽  
Biocontrol Agent ◽  
Biological Control Agent ◽  
Control Agent ◽  
Selective Medium ◽  
Trichoderma Spp ◽  
Its1 And Its2

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.

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