Biotechnology and Environmental applications of Trichoderma spp.

2021 ◽  
pp. 149-157
Author(s):  
Waill A. Elkhateeb ◽  
Marwa O. Elnahas ◽  
Ghoson M. Daba ◽  
Abdel-Nasser A. Zohri
Keyword(s):  
Biological Control ◽  
Agricultural Soils ◽  
Hydrolytic Enzymes ◽  
Biological Control Agents ◽  
Growth Promoters ◽  
Trichoderma Spp ◽  
Trichoderma Species ◽  
Plant Growth Promoters ◽  
Different Strains ◽  
Soil Borne Fungi

The genus Trichoderma is multicultural soil-borne fungi found in different ecosystems. They are highly successful colonizers of their habitats. Genus Trichoderma is capable of dealing with various environments such as compost, agricultural soils, rhizosphere, and waste material. Therefore, different strains of Trichoderma have been applied in agriculture, bioremediation, waste management, and biotechnology. Many Trichoderma species act as biological control agents and plant growth promoters. Additionally, the genus Trichoderma is a new fungal source for the production of cyclosporin A as well as various hydrolytic enzymes with industrial importance.

scholarly journals Exploration and Identification Trichoderma spp. as a Biological Control Agents to Plant Pathogens and Starter Making Biological Fertilizers

2020 ◽  
Vol 13 (1) ◽  
pp. 222-226
Author(s):  
Henny V.G. Makal ◽  
Max M. Ratulangi ◽  
Denny S. Sualang
Keyword(s):  
Biological Control ◽  
Population Density ◽  
Plant Pathogens ◽  
Soil Samples ◽  
Dilution Method ◽  
Biological Control Agents ◽  
Trichoderma Spp ◽  
Trichoderma Species ◽  
Trichoderma Sp ◽  
Biological Fertilizer

The objectives of this study are: (1) to inventory Trichoderma spp. in North Minahasa District, South Minahasa District, and Tomohon City-Minahasa District, (2) inventory of Trichoderma spp. in the rhizosphere of cultivated and fallow gardens, and (3) calculate the population density of Trichoderma spp. all soil samples. The scope of this study is the biological control of plant pathogens, induce plant resistance, and biological fertilizer production. Trichoderma isolation spp. has been carried out by dilution method and cultured on PDA + antibiotics. Population density of Trichoderma spp. calculated using the plate calculation method. Identification of this species function based on the color and patterns of sporulation in the colony; hyphae and clamydospores; conidiophores; and phialides and phialospores. Trichoderma species found in North Minahasa District were T. harzianum, T. koningii, and T. viride; in South Minahasa District, T. koningii and T. viride; and in Tomohon City-Minahasa District, T. koningii and T. viride. In fallow gardens were T. harzianum, T. koningii, and T. viride, and in cultivated gardens were T. koningii and T. viride. Population densities of Trichoderma sp. in South Minahasa District, North Minahasa District, and Tomohon City-Minahasa District, respectively 1,363.64, 466.67, and 26.67 CFU / g soil.

scholarly journals Biostimulants and Microorganisms Boost the Nutritional Composition of Buckwheat (Fagopyrum esculentum Moench) Sprouts

Agronomy ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 469 ◽  
Author(s):  
Robert Witkowicz ◽  
Wioletta Biel ◽  
Joanna Chłopicka ◽  
Agnieszka Galanty ◽  
Katarzyna Gleń-Karolczyk ◽  
...  
Keyword(s):  
Biological Control ◽  
Chemical Composition ◽  
Plant Growth ◽  
Nutritional Composition ◽  
Biological Control Agents ◽  
Growth Promoters ◽  
Pythium Oligandrum ◽  
Plant Growth Promoters ◽  
Fiber Fractions ◽  
Fagopyrum Esculentum Moench

This study investigated the influence of biological control agents and plant growth promoters on the chemical composition of the cultivars Panda and Kora buckwheat sprouts. Before sowing, seeds were soaked in solutions containing Bacillus subtilis bacteria, Pythium oligandrum oospores, Ecklonia maxima algae extract, and/or nitrophenols. The sprouts of the Panda displayed higher levels of protein, fat, and dietary fiber fractions than the Kora. Measurable effects of biological control agents (BCAs) and plant growth promoters (PGPs) on the chemical composition of sprouts were also confirmed. Soaking the seeds in a solution containing P. oligandrum oospores resulted in a decrease in the level of crude ash in sprouts, while the addition of nitrophenols increased the level of both crude ash and protein. We also found statistically significant effects of interactions between the cultivar and BCA and/or PGP for each of the examined components.

scholarly journals Trichoderma Secondary Metabolites that Affect Plant Metabolism

2012 ◽  
Vol 7 (11) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Francesco Vinale ◽  
Krishnapillai Sivasithamparam ◽  
Emilio L. Ghisalberti ◽  
Michelina Ruocco ◽  
Sheridan Woo ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Plant Growth ◽  
Biological Activities ◽  
Plant Metabolism ◽  
Growth Promoters ◽  
Plant Host ◽  
Trichoderma Spp ◽  
Positive Effects ◽  
Complex Interactions ◽  
Plant Growth Promoters

Recently, there have been many exciting new developments relating to the use of Trichoderma spp. as agents for biocontrol of pathogens and as plant growth promoters. Several mechanisms have been proposed to explain the positive effects of these microorganisms on the plant host. One factor that contributes to their beneficial biological activities is related to the wide variety of metabolites that they produce. These metabolites have been found not only to directly inhibit the growth and pathogenic activities of the parasites, but also to increase disease resistance by triggering the system of defence in the plant host. In addition, these metabolites are also capable of enhancing plant growth, which enables the plant to counteract the disease with compensatory vegetative growth by the augmented production of root and shoot systems. This review takes into account the Trichoderma secondary metabolites that affect plant metabolism and that may play an important role in the complex interactions of this biocontrol agent with the plant and pathogens.

scholarly journals Holistic approach to control Melolontha spp. in organic strawberry plantations

2020 ◽  
Vol 10 (S1) ◽  
pp. 13-22
Author(s):  
Eligio Malusá ◽  
Małgorzata Tartanus ◽  
Ewa M. Furmanczyk ◽  
Barbara H. Łabanowska
Keyword(s):  
Biological Control ◽  
Entomopathogenic Fungi ◽  
Predictive Models ◽  
Holistic Approach ◽  
Biological Control Agents ◽  
Light Traps ◽  
Biological Cycle ◽  
Effective Reduction ◽  
Different Strains ◽  
Root Feeding

AbstractTo achieve an effective reduction of the damage by root feeding grubs of Melolontha spp. in organic strawberry plantations, we have tested an approach targeting different stages of the insect’s biological cycle. Adult beetles were caught by using light traps or by manual shaking off trees associated to the monitoring of cockchafer swarm flights supported by forecasts models. Phytosanitary pre-crops and the application of biological control agents were tested against the larvae. The three predictive models utilized to forecast the period of emergence of the cockchafer were suitable to support the deployment of the light traps before the adults’ swarm flights. Traps positioned at 4-m height were more effective in attracting the beetles than those kept at 2-m height. Buckwheat in mixtures with either a mustard or leguminous species used as pre-crops was able to reduce the population of grubs, and considering also its capacity in solubilizing recalcitrant phosphorous sources should enter in a rotation with strawberry or any other crop susceptible to grubs damage. The distribution of two different strains of entomopathogenic fungi resulted in a reduction of the damage to plants due to the cockchafer grubs’ activity, even though the efficacy resulted to be dependent on environmental and agronomic factors, including the kind of formulation used. It is concluded that to assure a sufficient level of control of Melolontha spp. in organic strawberry plantations, it is necessary to integrate several methods that are targeting the different biological stages of the insect and are based on different kinds of practices.

scholarly journals Trichoderma Biological Control to Protect Sensitive Maize Hybrids Against Late Wilt Disease in the Field

2021 ◽  
Vol 7 (4) ◽  
pp. 315
Author(s):  
Ofir Degani ◽  
Shlomit Dor
Keyword(s):  
Biological Control ◽  
Growth Parameters ◽  
Crop Protection ◽  
Antagonistic Activity ◽  
Trichoderma Spp ◽  
Maize Crop ◽  
Trichoderma Species ◽  
Maize Cultivars ◽  
Pathogen Dna

Late wilt, a disease severely affecting maize fields throughout Israel, is characterized by the relatively rapid wilting of maize plants from the tasseling stage to maturity. The disease is caused by the fungus Magnaporthiopsis maydis, a soil and seed-borne pathogen. The pathogen is controlled traditionally through the use of maize cultivars having reduced sensitivity to the disease. Nevertheless, such cultivars may lose their immunity after several years of intensive growth due to the presence of high virulent isolates of M. maydis. Alternative effective and economical chemical treatment to the disease was recently established but is dependent on the use of a dripline assigned for two adjacent rows and exposes the risk of fungicide resistance. In the current work, eight marine and soil isolates of Trichoderma spp., known for high mycoparasitic potential, were tested as biocontrol agents against M. maydis. An in vitro confront plate assay revealed strong antagonistic activity against the pathogen of two T. longibrachiatum isolates and of T. asperelloides. These species produce soluble metabolites that can inhibit or kill the maize pathogen in submerged and solid media culture growth assays. In greenhouse experiments accompanied by real-time PCR tracking of the pathogen, the Trichoderma species or their metabolites managed to improve the seedlings’ wet biomass and reduced the pathogen DNA in the maize roots. A follow-up experiment carried out through a whole growth session, under field conditions, provided important support to the Trichoderma species’ beneficial impact. The direct addition of T. longibrachiatum and even more T. asperelloides to the seeds, with the sowing, resulted in a yield improvement, a significant increase in the growth parameters and crops, to the degree of noninfected plants. These bioprotective treatments also restricted the pathogen DNA in the host tissues (up to 98%) and prevented the disease symptoms. The results encourage more in-depth research to uncover such biological agents’ potential and the methods to implement them in commercial fields. If adequately developed into final products and combined with other control methods, the biological control could play an important role in maize crop protection against Late wilt.

scholarly journals Novel Insights into the Effect of Pythium Strains on Rapeseed Metabolism

2020 ◽  
Vol 8 (10) ◽  
pp. 1472
Author(s):  
Kateřina Bělonožníková ◽  
Kateřina Vaverová ◽  
Tomáš Vaněk ◽  
Miroslav Kolařík ◽  
Veronika Hýsková ◽  
...  
Keyword(s):  
Plant Growth ◽  
Biological Control Agent ◽  
Effective Strain ◽  
Hydrolytic Enzymes ◽  
Biochemical Properties ◽  
Control Agent ◽  
Growth Promoters ◽  
Pythium Oligandrum ◽  
Defense Compounds ◽  
Plant Growth Promoters

Pythium oligandrum is a unique biological control agent. This soil oomycete not only acts as a mycoparasite, but also interacts with plant roots and stimulates plant defense response via specific elicitors. In addition, P. oligandrum can synthetize auxin precursors and stimulate plant growth. We analyzed the secretomes and biochemical properties of eleven Pythium isolates to find a novel and effective strain with advantageous features for plants. Our results showed that even closely related P. oligandrum isolates significantly differ in the content of compounds secreted into the medium, and that all strains secrete proteins, amino acids, tryptamine, phenolics, and hydrolytic enzymes capable of degrading cell walls (endo-β-1,3-glucanase, chitinase, and cellulase), exoglycosidases (especially β-glucosidase), proteases, and phosphatases. The most different strain was identified as a not yet described Pythium species. The changes in metabolism of Brassica napus plants grown from seeds coated with the tested Pythium spp. were characterized. Enhanced levels of jasmonates, ethylene precursor, and salicylic acid may indicate better resistance to a wide variety of pathogens. Glucosinolates, as defense compounds against insects and herbivores, were enhanced in young plants. Altogether, P. oligandrum strains varied in their life strategies, and either they could perform equally as plant growth promoters and mycoparasites or they had developed one of these strategies better.

scholarly journals AKTIVITAS SIDEROFOR BACILLUS SUBTILIS SEBAGAI PEMACU PERTUMBUHAN DAN PENGENDALI PATOGEN TANAMAN TERUNG

2017 ◽  
Vol 17 (2) ◽  
pp. 170 ◽  
Author(s):  
Nur Prihatiningsih ◽  
Heru Adi Djatmiko ◽  
Puji Lestari
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Plant Growth ◽  
Biological Control Agent ◽  
Control Agent ◽  
Leaf Number ◽  
Growth Promoters ◽  
Root Volume ◽  
Volume Weight ◽  
Plant Growth Promoters

Siderophore activity of Bacillus subtilis as plant growth promoters and biological control agent of eggplants pathogens. The aims of this research were to identify the siderophores of B. subtilis, to assess its activities as plant growth promoters and biological control agent of eggplants pathogens. Five isolates of B. subtilis i.e.B46, B209, B211, B298 and B 315 grown on SDCASA medium. The isolate which showed the best siderophores production was then further studied on its ability as a growth promoter on eggplants in two soil types with different Fe content. The inhibitory test was conducted against two kinds of pathogens, namely Colletotrichum sp. and Ralstonia solanacearum. The greenhouse experiment was arranged using a factorial completely randomized block design. The first factor was the B. subtilis (B. subtilis B298 and without B. subtilis B298), second factor was the type of soil (Ultisol and Andisol). The variables measured were Fe uptake by plants, plant growth parameters on eggplant i.e. height, leaf number, root length, root volume, weight of fresh and dried shoot as well as fresh and dry root, percentage of inhibition to fungal and bacterial eggplant pathogens. The results showed that the five isolates of B. subtilis were able to produce siderophores as catecholate and hydroxamate types. The best siderophore production was showed by B. subtilis B298. The ability of B. subtilis B298 in accelerating the growth of plants was indicated by the increased of uptake Fe, plant height, leaf number, root volume, weight of dried plants by 45.62%, 25.48%, 19.45%, 41.10% and 34.89% respectively. The inhibition to the fungal and bacterial eggplant pathogens best shown by the isolates of B. subtilis B298 with 55.4% and 22 mm respectively.

Effets de concentrations élevées de sels sur la croissance, la sporulation et la pigmentation de Trichoderma spp.

1977 ◽  
Vol 23 (5) ◽  
pp. 607-616 ◽  
Author(s):  
D. Gindrat
Keyword(s):  
Biological Control ◽  
Osmotic Potential ◽  
Maximal Growth ◽  
High Concentration ◽  
Trichoderma Spp ◽  
Trichoderma Species ◽  
Gliocladium Virens ◽  
The Media ◽  
Agar Media

On agar media supplemented with NaCl (5% = 856 mM), seven Trichoderma species aggregates and an isolate intermediate between T. harzianum and T. viride (T. 410) grew more slowly, sporulated poorly, and there was no characteristic pigmentation of the colonies. NaCl or KCl concentrations inducing 'albinization' differed with different isolates. CaCl2 (90–270 mM) reversed the anti-sporulating effect of NaCl (856 mM) on T. 410, and stimulated conidiation in media with no NaCl added. Gliocladium virens, but not G. roseum, reacted like Trichoderma to NaCl. Fourteen other fungal genera offered various reactions. Since 856 mM NaCl decreases osmotic potential (π) of the media used from −1.2 to −41 bars, the influence of π on T. 410 was examined. After 3 to 4 days, maximal growth was observed between −1.2 and −10 bars. Growth was reduced to 50% at about −30 bars. No growth occurred at about −90 bars. No pigmentation was observed at −32 bars (NaCl) and −41 bars (KCl). Abundant sporulation and pigmentation occurred at −31 bars with CaCl2 as sole electrolyte added. Na+ was toxic at high concentration. Results are discussed in view of possible use of Trichoderma in biological control.

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