Hyaluronan-based cryogels are effective scaffolds for human dermal fibroblasts supporting long term growth and matrix deposition in vitro

Our previous study evaluated the antioxidant activities of sulfated polysaccharides from Celluclast-assisted extract of Hizikia fusiforme (HFPS) in vitro in Vero cells and in vivo in zebrafish. The results showed that HFPS possesses strong antioxidant activity and suggested the potential photo-protective activities of HFPS. Hence, in the present study, we investigated the protective effects of HFPS against ultraviolet (UV) B-induced skin damage in vitro in human dermal fibroblasts (HDF cells). The results indicate that HFPS significantly reduced intracellular reactive oxygen species (ROS) level and improved the viability of UVB-irradiated HDF cells in a dose-dependent manner. Furthermore, HFPS significantly inhibited intracellular collagenase and elastase activities, remarkably protected collagen synthesis, and reduced matrix metalloproteinases (MMPs) expression by regulating nuclear factor kappa B (NF-κB), activator protein 1 (AP-1), and mitogen-activated protein kinases (MAPKs) signaling pathways in UVB-irradiated HDF cells. These results suggest that HFPS possesses strong UV protective effect, and can be a potential ingredient in the pharmaceutical and cosmetic industries.

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Comparison of the Biological Behaviour of Human Dermal Fibroblasts seeded on 3D Printed Polylactic acid, Polycaprolactone and Polyethylene Terephthalate Scaffolds in vitro

Journal of Medical Innovation and Technology ◽

10.51934/jomit.957164 ◽

2021 ◽

Author(s):

Ufkay KARABAY ◽

R. Bugra HUSEMOGLU ◽

Mehtap YUKSEL EGRILMEZ ◽

Selma AYDEMİR ◽

Başak GÜZEL BAYKAYRA ◽

...

Keyword(s):

Polylactic Acid ◽

Polyethylene Terephthalate ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts ◽

Biological Behaviour ◽

3D Printed

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A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study

Environmental Health and Preventive Medicine ◽

10.1186/s12199-018-0730-3 ◽

2018 ◽

Vol 23 (1) ◽

Cited By ~ 1

Author(s):

Ken Shirato ◽

Jun Takanari ◽

Tomoko Koda ◽

Takuya Sakurai ◽

Junetsu Ogasawara ◽

...

Keyword(s):

Heat Shock ◽

Heat Shock Protein 70 ◽

In Vitro Study ◽

Dermal Fibroblasts ◽

Ultraviolet B ◽

Asparagus Officinalis ◽

Human Dermal Fibroblasts ◽

Normal Human ◽

Normal Human Dermal Fibroblasts

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Photobiomodulation with 590 nm Wavelength Delays the Telomere Shortening and Replicative Senescence of Human Dermal Fibroblasts In Vitro

Photobiomodulation Photomedicine and Laser Surgery ◽

10.1089/photob.2020.4822 ◽

2020 ◽

Vol 38 (11) ◽

pp. 656-660

Author(s):

Borislav Arabadjiev ◽

Roumen Pankov ◽

Ivelina Vassileva ◽

Lyuben Sashov Petrov ◽

Ivan Buchvarov

Keyword(s):

Replicative Senescence ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts ◽

Telomere Shortening

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Protective effect of diphlorethohydroxycarmalol isolated from Ishige okamurae against UVB-induced damage in vitro in human dermal fibroblasts and in vivo in zebrafish

Food and Chemical Toxicology ◽

10.1016/j.fct.2019.110963 ◽

2020 ◽

Vol 136 ◽

pp. 110963 ◽

Cited By ~ 9

Author(s):

Lei Wang ◽

Hyun Soo Kim ◽

Jae Young Oh ◽

Jun Geon Je ◽

You-Jin Jeon ◽

...

Keyword(s):

Protective Effect ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts ◽

Ishige Okamurae

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Effect of Protein Kinase C delta (PKC-δ) Inhibition on the Transcriptome of Normal and Systemic Sclerosis Human Dermal Fibroblasts In Vitro

PLoS ONE ◽

10.1371/journal.pone.0027110 ◽

2011 ◽

Vol 6 (11) ◽

pp. e27110 ◽

Cited By ~ 16

Author(s):

Peter J. Wermuth ◽

Sankar Addya ◽

Sergio A. Jimenez

Keyword(s):

Protein Kinase C ◽

Systemic Sclerosis ◽

Protein Kinase ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts ◽

Protein Kinase C Delta ◽

Kinase C

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Long-term fate of silica nanoparticles interacting with human dermal fibroblasts

Biomaterials ◽

10.1016/j.biomaterials.2012.03.004 ◽

2012 ◽

Vol 33 (17) ◽

pp. 4431-4442 ◽

Cited By ~ 50

Author(s):

Sandrine Quignard ◽

Gervaise Mosser ◽

Michel Boissière ◽

Thibaud Coradin

Keyword(s):

Silica Nanoparticles ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts

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Polycaprolactone-Chitin Nanofibrous Mats as Potential Scaffolds for Tissue Engineering

Journal of Nanomaterials ◽

10.1155/2012/635212 ◽

2012 ◽

Vol 2012 ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Min Sup Kim ◽

Sang Jun Park ◽

Bon Kang Gu ◽

Chun-Ho Kim

Keyword(s):

Electron Microscopy ◽

Tissue Engineering ◽

Contact Angles ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts ◽

Water Contact ◽

Mean Diameter ◽

Poly Caprolactone ◽

Scanning Electron

We describe here the preparation of poly(caprolactone) (PCL)-chitin nanofibrous mats by electrospinning from a blended solution of PCL and chitin dissolved in a cosolvent, 1,1,1,3,3,3-hexafluoro-2-propanol and trifluoroacetic acid. Scanning electron microscopy showed that the neutralized PCL-chitin nanofibrous mats were morphologically stable, with a mean diameter of340.5±2.6 nm, compared with a diameter of524.2±12.1 nm for PCL mats. The nanofibrous mats showed decreased water contact angles as the proportion of chitin increased. However, the tensile properties of nanofibrous mats containing30~50% (wt/wt) chitin were enhanced compared with PCL-only mats.In vitrostudies showed that the viability of human dermal fibroblasts (HDFs) for up to 7 days in culture was higher on composite (OD value:1.42±0.09) than on PCL-only (0.51±0.14) nanofibrous mats, with viability correlated with chitin concentration. Together, our results suggest that PCL-chitin nanofibrous mats can be used as an implantable substrate to modulate HDF viability in tissue engineering.

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