Comparison of the Biological Behaviour of Human Dermal Fibroblasts seeded on 3D Printed Polylactic acid, Polycaprolactone and Polyethylene Terephthalate Scaffolds in vitro

Our previous study evaluated the antioxidant activities of sulfated polysaccharides from Celluclast-assisted extract of Hizikia fusiforme (HFPS) in vitro in Vero cells and in vivo in zebrafish. The results showed that HFPS possesses strong antioxidant activity and suggested the potential photo-protective activities of HFPS. Hence, in the present study, we investigated the protective effects of HFPS against ultraviolet (UV) B-induced skin damage in vitro in human dermal fibroblasts (HDF cells). The results indicate that HFPS significantly reduced intracellular reactive oxygen species (ROS) level and improved the viability of UVB-irradiated HDF cells in a dose-dependent manner. Furthermore, HFPS significantly inhibited intracellular collagenase and elastase activities, remarkably protected collagen synthesis, and reduced matrix metalloproteinases (MMPs) expression by regulating nuclear factor kappa B (NF-κB), activator protein 1 (AP-1), and mitogen-activated protein kinases (MAPKs) signaling pathways in UVB-irradiated HDF cells. These results suggest that HFPS possesses strong UV protective effect, and can be a potential ingredient in the pharmaceutical and cosmetic industries.

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Suture Fiber Reinforcement of a 3D Printed Gelatin Scaffold for Its Potential Application in Soft Tissue Engineering

International Journal of Molecular Sciences ◽

10.3390/ijms222111600 ◽

2021 ◽

Vol 22 (21) ◽

pp. 11600

Author(s):

Dong Jin Choi ◽

Kyoung Choi ◽

Sang Jun Park ◽

Young-Jin Kim ◽

Seok Chung ◽

...

Keyword(s):

Tissue Engineering ◽

Mechanical Strength ◽

Physical Properties ◽

Dimensional Stability ◽

3D Structure ◽

Biological Properties ◽

Dermal Fibroblasts ◽

3D Scaffolds ◽

3D Printed

Gelatin has excellent biological properties, but its poor physical properties are a major obstacle to its use as a biomaterial ink. These disadvantages not only worsen the printability of gelatin biomaterial ink, but also reduce the dimensional stability of its 3D scaffolds and limit its application in the tissue engineering field. Herein, biodegradable suture fibers were added into a gelatin biomaterial ink to improve the printability, mechanical strength, and dimensional stability of the 3D printed scaffolds. The suture fiber reinforced gelatin 3D scaffolds were fabricated using the thermo-responsive properties of gelatin under optimized 3D printing conditions (−10 °C cryogenic plate, 40–80 kPa pneumatic pressure, and 9 mm/s printing speed), and were crosslinked using EDC/NHS to maintain their 3D structures. Scanning electron microscopy images revealed that the morphologies of the 3D printed scaffolds maintained their 3D structure after crosslinking. The addition of 0.5% (w/v) of suture fibers increased the printing accuracy of the 3D printed scaffolds to 97%. The suture fibers also increased the mechanical strength of the 3D printed scaffolds by up to 6-fold, and the degradation rate could be controlled by the suture fiber content. In in vitro cell studies, DNA assay results showed that human dermal fibroblasts’ proliferation rate of a 3D printed scaffold containing 0.5% suture fiber was 10% higher than that of a 3D printed scaffold without suture fibers after 14 days of culture. Interestingly, the supplement of suture fibers into gelatin biomaterial ink was able to minimize the cell-mediated contraction of the cell cultured 3D scaffolds over the cell culture period. These results show that advanced biomaterial inks can be developed by supplementing biodegradable fibers to improve the poor physical properties of natural polymer-based biomaterial inks.

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A standardized extract of Asparagus officinalis stem prevents reduction in heat shock protein 70 expression in ultraviolet-B-irradiated normal human dermal fibroblasts: an in vitro study

Environmental Health and Preventive Medicine ◽

10.1186/s12199-018-0730-3 ◽

2018 ◽

Vol 23 (1) ◽

Cited By ~ 1

Author(s):

Ken Shirato ◽

Jun Takanari ◽

Tomoko Koda ◽

Takuya Sakurai ◽

Junetsu Ogasawara ◽

...

Keyword(s):

Heat Shock ◽

Heat Shock Protein 70 ◽

In Vitro Study ◽

Dermal Fibroblasts ◽

Ultraviolet B ◽

Asparagus Officinalis ◽

Human Dermal Fibroblasts ◽

Normal Human ◽

Normal Human Dermal Fibroblasts

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Photobiomodulation with 590 nm Wavelength Delays the Telomere Shortening and Replicative Senescence of Human Dermal Fibroblasts In Vitro

Photobiomodulation Photomedicine and Laser Surgery ◽

10.1089/photob.2020.4822 ◽

2020 ◽

Vol 38 (11) ◽

pp. 656-660

Author(s):

Borislav Arabadjiev ◽

Roumen Pankov ◽

Ivelina Vassileva ◽

Lyuben Sashov Petrov ◽

Ivan Buchvarov

Keyword(s):

Replicative Senescence ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts ◽

Telomere Shortening

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Protective effect of diphlorethohydroxycarmalol isolated from Ishige okamurae against UVB-induced damage in vitro in human dermal fibroblasts and in vivo in zebrafish

Food and Chemical Toxicology ◽

10.1016/j.fct.2019.110963 ◽

2020 ◽

Vol 136 ◽

pp. 110963 ◽

Cited By ~ 9

Author(s):

Lei Wang ◽

Hyun Soo Kim ◽

Jae Young Oh ◽

Jun Geon Je ◽

You-Jin Jeon ◽

...

Keyword(s):

Protective Effect ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts ◽

Ishige Okamurae

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Effect of Protein Kinase C delta (PKC-δ) Inhibition on the Transcriptome of Normal and Systemic Sclerosis Human Dermal Fibroblasts In Vitro

PLoS ONE ◽

10.1371/journal.pone.0027110 ◽

2011 ◽

Vol 6 (11) ◽

pp. e27110 ◽

Cited By ~ 16

Author(s):

Peter J. Wermuth ◽

Sankar Addya ◽

Sergio A. Jimenez

Keyword(s):

Protein Kinase C ◽

Systemic Sclerosis ◽

Protein Kinase ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts ◽

Protein Kinase C Delta ◽

Kinase C

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Polycaprolactone-Chitin Nanofibrous Mats as Potential Scaffolds for Tissue Engineering

Journal of Nanomaterials ◽

10.1155/2012/635212 ◽

2012 ◽

Vol 2012 ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Min Sup Kim ◽

Sang Jun Park ◽

Bon Kang Gu ◽

Chun-Ho Kim

Keyword(s):

Electron Microscopy ◽

Tissue Engineering ◽

Contact Angles ◽

Dermal Fibroblasts ◽

Human Dermal Fibroblasts ◽

Water Contact ◽

Mean Diameter ◽

Poly Caprolactone ◽

Scanning Electron

We describe here the preparation of poly(caprolactone) (PCL)-chitin nanofibrous mats by electrospinning from a blended solution of PCL and chitin dissolved in a cosolvent, 1,1,1,3,3,3-hexafluoro-2-propanol and trifluoroacetic acid. Scanning electron microscopy showed that the neutralized PCL-chitin nanofibrous mats were morphologically stable, with a mean diameter of340.5±2.6 nm, compared with a diameter of524.2±12.1 nm for PCL mats. The nanofibrous mats showed decreased water contact angles as the proportion of chitin increased. However, the tensile properties of nanofibrous mats containing30~50% (wt/wt) chitin were enhanced compared with PCL-only mats.In vitrostudies showed that the viability of human dermal fibroblasts (HDFs) for up to 7 days in culture was higher on composite (OD value:1.42±0.09) than on PCL-only (0.51±0.14) nanofibrous mats, with viability correlated with chitin concentration. Together, our results suggest that PCL-chitin nanofibrous mats can be used as an implantable substrate to modulate HDF viability in tissue engineering.

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Validation of in vitro assays in three-dimensional human dermal constructs

The International Journal of Artificial Organs ◽

10.1177/0391398818775519 ◽

2018 ◽

Vol 41 (11) ◽

pp. 779-788 ◽

Cited By ~ 3

Author(s):

Ayesha Idrees ◽

Valeria Chiono ◽

Gianluca Ciardelli ◽

Siegfried Shah ◽

Richard Viebahn ◽

...

Keyword(s):

Three Dimensional ◽

Dermal Fibroblasts ◽

In Vitro Assays ◽

Dimensional System ◽

Type I ◽

Human Dermal Fibroblasts ◽

Cell Viability Assay ◽

Normal Human ◽

Normal Human Dermal Fibroblasts

Three-dimensional cell culture systems are urgently needed for cytocompatibility testing of biomaterials. This work aimed at the development of three-dimensional in vitro dermal skin models and their optimization for cytocompatibility evaluation. Initially “murine in vitro dermal construct” based on L929 cells was generated, leading to the development of “human in vitro dermal construct” consisting of normal human dermal fibroblasts in rat tail tendon collagen type I. To assess the viability of the cells, different assays CellTiter-Blue®, RealTime-Glo™ MT, and CellTiter-Glo® (Promega) were evaluated to optimize the best-suited assay to the respective cell type and three-dimensional system. Z-stack imaging (Live/Dead and Phalloidin/DAPI-Promokine) was performed to visualize normal human dermal fibroblasts inside matrix revealing filopodia-like morphology and a uniform distribution of normal human dermal fibroblasts in matrix. CellTiter-Glo was found to be the optimal cell viability assay among those analyzed. CellTiter-Blue reagent affected the cell morphology of normal human dermal fibroblasts (unlike L929), suggesting an interference with cell biological activity, resulting in less reliable viability data. On the other hand, RealTime-Glo provided a linear signal only with a very low cell density, which made this assay unsuitable for this system. CellTiter-Glo adapted to three-dimensional dermal construct by optimizing the “shaking time” to enhance the reagent penetration and maximum adenosine triphosphate release, indicating 2.4 times higher viability value by shaking for 60 min than for 5 min. In addition, viability results showed that cells were viable inside the matrix. This model would be further advanced with more layers of skin to make a full thickness model.

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Antiphotoaging Effect of 3,5-Dicaffeoyl-epi-quinic Acid against UVA-Induced Skin Damage by Protecting Human Dermal Fibroblasts In Vitro

International Journal of Molecular Sciences ◽

10.3390/ijms21207756 ◽

2020 ◽

Vol 21 (20) ◽

pp. 7756

Author(s):

Jung Hwan Oh ◽

Fatih Karadeniz ◽

Chang-Suk Kong ◽

Youngwan Seo

Keyword(s):

Molecular Mechanisms ◽

Type I Collagen ◽

Nuclear Translocation ◽

Quinic Acid ◽

Dermal Fibroblasts ◽

Type I ◽

Human Dermal Fibroblasts ◽

Skin Damage ◽

Chronological Aging

Cutaneous aging is divided into intrinsic and exogenous aging correspondingly contributing to the complex biological phenomenon in skin. Intrinsic aging is also termed chronological aging, which is the accumulation of inevitable changes over time and is largely genetically determined. Superimposed on this intrinsic process, exogenous aging is associated with environmental exposure, mainly to ultraviolet (UV) radiation and more commonly termed as photoaging. UV-induced skin aging induces increased expression of matrix metalloproteinases (MMPs) which in turn causes the collagen degradation. Therefore, MMP inhibitors of natural origin are regarded as a primary approach to prevent or treat photoaging. This study investigated the effects of 3,5-dicaffeoyl-epi-quinic acid (DEQA) on photoaging and elucidated its molecular mechanisms in UVA-irradiated human dermal fibroblasts (HDFs). The results show that treatment with DEQA decreases MMP-1 production and increases type I collagen production in UVA-damaged HDFs. In addition, treatment of UVA-irradiated HDFs with DEQA downregulates MMP-1, MMP-3 and MMP-9 expression via blocking MAPK-cascade-regulated AP-1 transcriptional activity in UVA-irradiated HDFs. Furthermore, DEQA relieves the UVA-mediated suppression of type I procollagen and collagen expression through stimulating TGF-β/Smad signaling, leading to activation of the Smad 2/3 and Smad 4 nuclear translocation. These results suggest that DEQA could be a potential cosmetic agent for prevention and treatment of skin photoaging.

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