Antibacterial Evaluation of Lithium-Loaded Nanofibrous Poly(L-Lactic Acid) Membranes Fabricated via an Electrospinning Strategy

Lithium (Li) reportedly has anti-bacterial properties. Thus, it is an ideal option to modify barrier membranes used for guided bone regeneration to inhibit the bacterial adhesion. The aims of this study were to fabricate and characterize nanofibrous poly(L-lactic acid) (PLLA) membranes containing Li, and investigate their antibacterial effects on Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans in vitro. Li (5%Li, 10%Li, and 15%Li)-loaded nanofibrous PLLA membranes were fabricated using an electrospinning technique, and characterized via scanning electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction, a contact angle measuring device, and a universal testing machine. Sustained release of Li ions was measured over a 14-day period and biocompatibility of the Li-PLLA membranes was investigated. Evaluation of bacterial adhesion and antibacterial activity were conducted by bacterial colony counting, LIVE/DEAD staining and inhibition zone method using P.gingivalis and A.actinomycetemcomitans. Of the three Li-loaded membranes assessed, the 10%Li-PLLA membrane had the best mechanical properties and biocompatibility. Adhesion of both P.gingivalis and A.actinomycetemcomitans on Li-PLLA membranes was significantly lower than adhesion on pure PLLA membranes, particularly with regard to the 10%Li and 15%Li membranes. Significant antibacterial activity of Li-PLLA were also observed against according to the inhibition zone test. Given their better mechanical properties, biocompatibility, and antibacterial activity, PLLAs with 10%Li are a better choice for future clinical utilization. The pronounced antibacterial effects of Li-loaded PLLA membranes sets the stage for further application in guided bone regeneration.

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Zn-Containing Membranes for Guided Bone Regeneration in Dentistry

Polymers ◽

10.3390/polym13111797 ◽

2021 ◽

Vol 13 (11) ◽

pp. 1797

Author(s):

Manuel Toledano ◽

Marta Vallecillo-Rivas ◽

María T. Osorio ◽

Esther Muñoz-Soto ◽

Manuel Toledano-Osorio ◽

...

Keyword(s):

Mechanical Properties ◽

Bone Regeneration ◽

Bone Healing ◽

Bone Repair ◽

Complex Modulus ◽

Bacterial Colonization ◽

Guided Bone Regeneration ◽

M2 Macrophage

Barrier membranes are employed in guided bone regeneration (GBR) to facilitate bone in-growth. A bioactive and biomimetic Zn-doped membrane with the ability to participate in bone healing and regeneration is necessary. The aim of the present study is to state the effect of doping the membranes for GBR with zinc compounds in the improvement of bone regeneration. A literature search was conducted using electronic databases, such as PubMed, MEDLINE, DIMDI, Embase, Scopus and Web of Science. A narrative exploratory review was undertaken, focusing on the antibacterial effects, physicochemical and biological properties of Zn-loaded membranes. Bioactivity, bone formation and cytotoxicity were analyzed. Microstructure and mechanical properties of these membranes were also determined. Zn-doped membranes have inhibited in vivo and in vitro bacterial colonization. Zn-alloy and Zn-doped membranes attained good biocompatibility and were found to be non-toxic to cells. The Zn-doped matrices showed feasible mechanical properties, such as flexibility, strength, complex modulus and tan delta. Zn incorporation in polymeric membranes provided the highest regenerative efficiency for bone healing in experimental animals, potentiating osteogenesis, angiogenesis, biological activity and a balanced remodeling. Zn-loaded membranes doped with SiO2 nanoparticles have performed as bioactive modulators provoking an M2 macrophage increase and are a potential biomaterial for promoting bone repair. Zn-doped membranes have promoted pro-healing phenotypes.

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EFFECT OF GARLIC POWDER ADDITION TO GELATIN BIOCOMPOSITE ON ITS ANTIBACTERIAL ACTIVITY

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.12.1.930.1-13 ◽

2016 ◽

Vol 12 (1) ◽

pp. 1

Author(s):

Pramudita Putri Kusuma ◽

Ganjar Fadillah ◽

Husna Syaima ◽

Teguh Endah Saraswati

Keyword(s):

Antibacterial Activity ◽

Lactic Acid ◽

Weight Ratio ◽

Inhibition Zone ◽

Diffusion Method ◽

Powder Preparation ◽

Natural Preservative ◽

Garlic Powder ◽

Optimum Diameter ◽

Powder Addition

The addition of garlic powder to gelatin from chicken claw waste was potentially developed as a natural preservative in food, especially for meat. Preparation of gelatin/garlic biocomposite was performed in three stages: synthesis of gelatin from chicken claw, garlic powder preparation as allicin source and preparation of biocomposite gelatin/garlic. The preparation of dry biocomposites was done by weighing the gelatin and garlic powder in weight ratio of 1 : 1 and 1 : 2 (w/w) in the total mass of 0.75 grams. For wet biocomposite preparation, the mixture of the powder was solved in 5 mL of lactic acid 2 %. Functional groups of gelatin, garlic and biocomposite were analyzed by fourier transform infrared spectroscopy (FTIR). The antibacterial activity of biocomposite against Staphylococcus aureus were tested using disc diffusion method. This test was performed on garlic powder, solvent and gelatin/garlic biocomposites powder in the ratio of 1 : 1 and 1 : 2 in 2 % lactic acid solvent. The biocomposite with a weight ratio of gelatin : garlic of 1 : 1 had the optimum diameter of inhibition zone. The effectiveness of biocomposite gelatin/garlic as natural preservative applied in meat was also physically studied by organoleptic analysis. Organoleptic analysis through the hedonic test was conducted on the parameters of color, smell, and texture of gelatin/garlic biocomposites-coated meat. The results showed that the addition of garlic can increase the effectiveness of gelatin as a natural preservative of meat for four days stored in closed packaging at room temperature.

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Carbonated Hydroxyapatite Containing Propolis as an Antibacterial Agent Candidate against Aggregatibacter actinomycetemcomitans

Majalah Obat Tradisional ◽

10.22146/mot.62712 ◽

2020 ◽

Vol 25 (3) ◽

Author(s):

Ayunda Nur Sukmawati ◽

Sri Pramestri ◽

Al. Sri Koes Soesilowati ◽

Suryono Suryono

Keyword(s):

Antibacterial Activity ◽

Bacterial Adhesion ◽

Antibacterial Agent ◽

Alveolar Bone ◽

Inhibition Zone ◽

Aggregatibacter Actinomycetemcomitans ◽

Test Method ◽

Graft Material ◽

Carbonated Hydroxyapatite ◽

Significant Difference

One of the periodontal pathogenic bacteria that can cause periodontitis and alveolar bone destruction is Aggregatibacter actinomycetemcomitans. An alveolar bone defect can be treated using a bone graft. Carbonated hydroxyapatite (CHA) is an alloplastic graft material. Alloplastic materials do not have vascularization, which will increase the risk of bacterial adhesion. Therefore, adding an antibacterial agent is needed to prevent bacterial adhesion, which will improve periodontal healing. Propolis is a natural ingredient that has broad-spectrum antibacterial activity and does not cause bacterial resistance. This study aimed to assess the antibacterial activity of carbonated hydroxyapatite after being incorporated with propolis against Aggregatibacter actinomycetemcomitans. Carbonated hydroxyapatite was embedded into four different concentrations of propolis solution (2.5%, 5%, 7.5%, and 10%). An antimicrobial assay against Aggregatibacter actinomycetemcomitans was done using the disc diffusion test method. The inhibition zone was measured to determine the antibacterial ability of the specimens. The inhibition zone was found on the carbonated hydroxyapatite incorporated with propolis at all concentrations. Carbonated hydroxyapatite incorporated with 10% propolis showed the largest inhibition zone. Data analysis using the Kruskal–Wallis test showed a significant difference between the groups tested (p <.05). In conclusion, carbonated hydroxyapatite incorporated with propolis has antibacterial activity against Aggregatibacter actinomycetemcomitans

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Antibacterial Activity of Nickel -Doped ZnO\MWCNTs hybrid Prepared by Sol-Gel technique

The European Physical Journal Applied Physics ◽

10.1051/epjap/2021210115 ◽

2021 ◽

Author(s):

Selma M.H. AL-Jawad ◽

Zahraa S. Shakir ◽

Duha S. Ahmed

Keyword(s):

Antibacterial Activity ◽

Sol Gel ◽

Inhibition Zone ◽

Sem Analysis ◽

Morphological Properties ◽

X Ray Diffraction ◽

Zno Nps ◽

X Ray ◽

Vis Spectroscopy ◽

Doped Zno

ZnO/MWCNTs hybrid and doped with different concentration of Nickel element prepared by using Sol-gel been technique reported. All samples were prepared and characterized by X-Ray Diffraction Analysis (XRD), Energy Dispersive X-ray Spectroscopy (EDS), Fourier-Transform Infrared Spectroscopy (FTIR), Field-Emission Scanning Electron Microscopy (FE-SEM), and UV-Vis spectroscopy have been identified the structural, optical and morphological properties. X-ray diffraction showed the polycrystalline nature with hexagonal wutzite structure of hybrid and doped with Nickel. The crystalline size of the hybrid nanostructure was increasing from 23.73 nm to 34.59 nm. Besides, the UV-Vis spectroscopy showed a significant decrease in the band gap values from 2.97 eV to 2.01 eV. Whereas the FE-SEM analysis confirm the formation spherical shapes of ZnO NPs deposited on cylindrical tubes representing the MWCNTs. The antibacterial activity reveals that the inhibition zone of Ni doped-ZnO/MWCNTs hybrid was 28.5 mm, 26.5 mm toward E. coli and S. aureus bacteria, respectively.

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Structure and properties of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)/poly(L-lactic acid) quasi core/sheath melt-electrospun microfibers

Textile Research Journal ◽

10.1177/0040517518779997 ◽

2018 ◽

Vol 89 (9) ◽

pp. 1770-1781 ◽

Cited By ~ 1

Author(s):

Huaizhong Xu ◽

Benedict Bauer ◽

Masaki Yamamoto ◽

Hideki Yamane

Keyword(s):

Mechanical Properties ◽

Tensile Strength ◽

Lactic Acid ◽

Tensile Tests ◽

Processing Parameters ◽

Structure And Properties ◽

X Ray Diffraction ◽

Scanning Calorimetry ◽

X Ray ◽

Diffraction Patterns

A facile route was proposed to fabricate core–sheath microfibers, and the relationships among processing parameters, crystalline structures and the mechanical properties were investigated. The compression molded poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBH)/poly(L-lactic acid) (PLLA) strip enhanced the spinnability of PHBH and the mechanical properties of PLLA as well. The core–sheath ratio of the fibers was determined by the prefab strip, while the PLLA sheath component did not completely cover the PHBH core component due to the weak interfacial tension between the melts of PHBH and PLLA. A rotational target was applied to collect aligned fibers, which were further drawn in a water bath. The tensile strength and the modulus of as-spun and drawn fibers increased with increasing the take-up velocities. When the take-up velocity was above 500 m/min, the jet became unstable and started to break up at the tip of the Taylor cone, decreasing the mechanical properties of the fibers. The drawing process facilitated the crystallization of PLLA and PHBH, and the tensile strength and the modulus increased linearly with the increasing the draw ratio. The crystal information displayed from wide-angle X-ray diffraction patterns and differential scanning calorimetry heating curves supported the results of the tensile tests.

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Preparation of Thermoplastic Poly(L-Lactic Acid) Membranes for Guided Bone Regeneration

The International Journal of Oral & Maxillofacial Implants ◽

10.11607/jomi.2729 ◽

2013 ◽

Vol 28 (4) ◽

pp. 973-981 ◽

Cited By ~ 3

Author(s):

Kazunari Asano ◽

Tomonori Matsuno ◽

Yasuhiko Tabata ◽

Tazuko Satoh

Keyword(s):

Lactic Acid ◽

Bone Regeneration ◽

Guided Bone Regeneration

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EFFECT OF GARLIC POWDER ADDITION TO GELATIN BIOCOMPOSITE ON ITS ANTIBACTERIAL ACTIVITY

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.v12i1.786 ◽

2016 ◽

Vol 12 (1) ◽

Author(s):

Pramudita Putri Kusuma

Keyword(s):

Antibacterial Activity ◽

Lactic Acid ◽

Weight Ratio ◽

Inhibition Zone ◽

Diffusion Method ◽

Powder Preparation ◽

Natural Preservative ◽

Garlic Powder ◽

Optimum Diameter ◽

Powder Addition

The addition of garlic powder to gelatin from chicken claw waste was potentially developed as a natural preservative in food, especially for meat. Preparation of gelatin/garlic biocomposite was performed in three stages: synthesis of gelatin from chicken claw, garlic powder preparation as allicin source and preparation of biocomposite gelatin/garlic. The preparation of dry biocomposites was done by weighing the gelatin and garlic powder in weight ratio of 1 : 1 and 1 : 2 (w/w) in the total mass of 0.75 grams. For wet biocomposite preparation, the mixture of the powder was solved in 5 mL of lactic acid 2 %. Functional groups of gelatin, garlic and biocomposite were analyzed by fourier transform infrared spectroscopy (FTIR). The antibacterial activity of biocomposite against Staphylococcus aureus were tested using disc diffusion method. This test was performed on garlic powder, solvent and gelatin/garlic biocomposites powder in the ratio of 1 : 1 and 1 : 2 in 2 % lactic acid solvent. The biocomposite with a weight ratio of gelatin : garlic of 1 : 1 had the optimum diameter of inhibition zone. The effectiveness of biocomposite gelatin/garlic as natural preservative applied in meat was also physically studied by organoleptic analysis. Organoleptic analysis through the hedonic test was conducted on the parameters of color, smell, and texture of gelatin/garlic biocomposites-coated meat. The results showed that the addition of garlic can increase the effectiveness of gelatin as a natural preservative of meat for four days stored in closed packaging at room temperature.

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Safety and feasibility assessment of biodegradable poly (l-lactic acid/ε-caprolactone) membrane for guided bone regeneration: A case series of first-in-human pilot study

Journal of Dental Sciences ◽

10.1016/j.jds.2021.08.015 ◽

2021 ◽

Author(s):

Kinuko Ogata ◽

Seigo Ohba ◽

Yoshinori Sumita ◽

Izumi Ashahina

Keyword(s):

Lactic Acid ◽

Pilot Study ◽

Bone Regeneration ◽

Case Series ◽

Guided Bone Regeneration ◽

Feasibility Assessment ◽

Biodegradable Poly

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The Promotion of Antibacterial Effects of Ti6Al4V Alloy Modified with TiO2Nanotubes Using a Superoxidized Solution

Journal of Nanomaterials ◽

10.1155/2015/818565 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Ernesto Beltrán-Partida ◽

Benjamin Valdez-Salas ◽

Alan Escamilla ◽

Aldo Moreno-Ulloa ◽

Larysa Burtseva ◽

...

Keyword(s):

Bacterial Adhesion ◽

Sem Analysis ◽

Ti6al4v Alloy ◽

Antibacterial Efficacy ◽

Antibacterial Effects ◽

X Ray ◽

Force Microscopy ◽

Atomic Force ◽

Bacterial Morphology

The purpose of the present study was to synthetize 80 nm diameter TiO2 nanotubes (NTs) on Ti6Al4V alloy using a commercially superoxidized water (SOW) enriched with fluoride to reduce anodization time and promote the antibacterial efficacy againstStaphylococcus aureus(S. aureus). The alloy discs were anodized for 5 min and as a result, NTs of approximately 80 nm diameters were obtained with similar morphology as reported in previous studies using longer anodization times (1-2 h). Filed emission-scanning electron microscopy (FE-SEM) and energy dispersive X-ray spectroscopy (EDX) were used to characterize the materials surfaces. The NTs showed significantly decreasedS. aureusviability after 1, 3, and 5 days of culture in comparison to nonanodized alloy. Likewise, SEM analysis also suggested lower bacterial adhesion on the NTs surface. No differences in bacterial morphology and topography were observed on both materials, as analyzed by SEM and atomic force microscopy (AFM). In conclusion, 80 nm diameter NTs were grown on Ti6Al4V alloy in 5 min by using a SOW solution enriched with fluoride, which resulted in a material with promoted antibacterial efficacy againstS. aureusfor up to 5 days of in vitro culture when compared to nonanodized alloy.

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