scholarly journals Biocompatible Mesoporous Silica–Polydopamine Nanocomplexes as MR/Fluorescence Imaging Agent for Light-Activated Photothermal–Photodynamic Cancer Therapy In Vivo

2021 ◽  
Vol 9 ◽  
Author(s):  
Jiahui Lu ◽  
Chen Ni ◽  
Jie Huang ◽  
Yawen Liu ◽  
Yingkai Tao ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Mesoporous Silica ◽  
Fluorescence Imaging ◽  
Colloidal Stability ◽  
Silica Nanoparticle ◽  
Photothermal Conversion ◽  
Normal Tissues ◽  
Irradiation Power

Conventional cancer phototherapy with single modality suffers from low therapeutic efficacy and undesired posttreatment damage for adjacent normal tissues. Therefore, the lower NIR laser irradiation power is vital to the reduction or preclusion of risk of scalds and burns in normal tissues. Herein, we rationally proposed a novel multifunctional nanocomplex, which enabled good magnetic resonance (MR) imaging contrast effect and promising photothermal conversion efficacy. The prepared core/shell nanocomplexes [MSN-Ce6@PDA (Mn)] were composed of chlorin e6-embedded mesoporous silica/nanoparticle composites as the cores, and then polydopamine and manganese ions were conjugated on the cores to form protective shells. The MSN-Ce6@PDA (Mn) nanocomplexes revealed superior properties in colloidal stability, photothermal conversion, reaction oxygen species generation, magnetic resonance imaging, etc. Under the guidance of MR and fluorescence imaging, these MSN-Ce6@PDA (Mn) nanocomplexes were found to be primarily accumulated in the MDA-MB-231 tumor area. Furthermore, the combined photodynamic and photothermal therapy exhibited strong inhibition to the growth of MDA-MB-231 tumor in vitro and in vivo. Besides, the MSN-Ce6@PDA (Mn) nanocomplexes also exhibited excellent biocompatibility and low damage to the healthy animals. Hence, the results demonstrated that the prepared MSN-Ce6@PDA (Mn) nanocomplex would be a promising potential for multimodal imaging-guided phototherapy.

scholarly journals Iron Oxide Incorporated Conjugated Polymer Nanoparticles for Simultaneous Use in Magnetic Resonance and Fluorescent Imaging of Brain Tumors

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1258
Author(s):  
N. Arias-Ramos ◽  
L. E. Ibarra ◽  
M. Serrano-Torres ◽  
B. Yagüe ◽  
M. D. Caverzán ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Conjugated Polymer ◽  
Colloidal Stability ◽  
Fluorescent Imaging ◽  
Polymer Nanoparticles ◽  
Biodistribution Studies ◽  
Conjugated Polymer Nanoparticles ◽  
Theranostic Agents

Conjugated polymer nanoparticles (CPNs) have emerged as advanced polymeric nanoplatforms in biomedical applications by virtue of extraordinary properties including high fluorescence brightness, large absorption coefficients of one and two-photons, and excellent photostability and colloidal stability in water and physiological medium. In addition, low cytotoxicity, easy functionalization, and the ability to modify CPN photochemical properties by the incorporation of dopants, convert them into excellent theranostic agents with multifunctionality for imaging and treatment. In this work, CPNs were designed and synthesized by incorporating a metal oxide magnetic core (Fe3O4 and NiFe2O4 nanoparticles, 5 nm) into their matrix during the nanoprecipitation method. This modification allowed the in vivo monitoring of nanoparticles in animal models using magnetic resonance imaging (MRI) and intravital fluorescence, techniques widely used for intracranial tumors evaluation. The modified CPNs were assessed in vivo in glioblastoma (GBM) bearing mice, both heterotopic and orthotopic developed models. Biodistribution studies were performed with MRI acquisitions and fluorescence images up to 24 h after the i.v. nanoparticles administration. The resulting IONP-doped CPNs were biocompatible in GBM tumor cells in vitro with an excellent cell incorporation depending on nanoparticle concentration exposure. IONP-doped CPNs were detected in tumor and excretory organs of the heterotopic GBM model after i.v. and i.t. injection. However, in the orthotopic GBM model, the size of the nanoparticles is probably hindering a higher effect on intratumorally T2-weighted images (T2WI) signals and T2 values. The photodynamic therapy (PDT)—cytotoxicity of CPNs was not either affected by the IONPs incorporation into the nanoparticles.

A redox-responsive mesoporous silica based nanoplatform for in vitro tumor-specific fluorescence imaging and enhanced photodynamic therapy

2018 ◽  
Vol 6 (1) ◽  
pp. 96-100 ◽  
Author(s):  
Fan Yuan ◽  
Jiang-Lan Li ◽  
Han Cheng ◽  
Xuan Zeng ◽  
Xian-Zheng Zhang
Keyword(s):  
Photodynamic Therapy ◽  
Tumor Microenvironment ◽  
Mesoporous Silica ◽  
Fluorescence Imaging ◽  
Silica Nanoparticle ◽  
Mesoporous Silica Nanoparticle ◽  
Specific Fluorescence ◽  
Redox Responsive ◽  
Multifunctional Peptides

A mesoporous silica nanoparticle-based redox-responsive platform modified with multifunctional peptides was successfully developed for in vitro tumor microenvironment-enhanced photodynamic therapy.

scholarly journals Retro-enantio isomer of angiopep-2 assists nanoprobes across the blood-brain barrier for targeted magnetic resonance/fluorescence imaging of glioblastoma

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Ruoxi Xie ◽  
Zijun Wu ◽  
Fanxin Zeng ◽  
Huawei Cai ◽  
Dan Wang ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Ex Vivo ◽  
Malignant Gliomas ◽  
Resonance Fluorescence ◽  
Malignant Tumours ◽  
Nir Fluorescence

AbstractGlioblastoma (GBM), one of the most common primary intracranial malignant tumours, is very difficult to be completely excised by surgery due to its irregular shape. Here, we use an MRI/NIR fluorescence dual-modal imaging nanoprobe that includes superparamagnetic iron oxide nanoparticles (SPIONs) modified with indocyanine (Cy7) molecules and peptides (ANG or DANG) to locate malignant gliomas and guide accurate excision. Both peptides/Cy7-SPIONs probes displayed excellent tumour-homing properties and barrier penetrating abilities in vitro, and both could mediate precise aggregation of the nanoprobes at gliomas sites in in vivo magnetic resonance imaging (MRI) and ex vivo near-infrared (NIR) fluorescence imaging. However, compared with ANG/Cy7-SPIONs probes, DANG/Cy7-SPIONs probes exhibited better enhanced MR imaging effects. Combining all these features together, this MRI/NIR fluorescence imaging dual-modal nanoprobes modified with retro-enantio isomers of the peptide have the potential to accurately display GBMs preoperatively for precise imaging and intraoperatively for real-time imaging.

Intratumoral H2O2-triggered release of CO from a metal carbonyl-based nanomedicine for efficient CO therapy

2017 ◽  
Vol 53 (40) ◽  
pp. 5557-5560 ◽  
Author(s):  
Zhaokui Jin ◽  
Yanyuan Wen ◽  
Liwei Xiong ◽  
Tian Yang ◽  
Penghe Zhao ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Metal Carbonyl ◽  
Silica Nanoparticle ◽  
Triggered Release ◽  
Mesoporous Silica Nanoparticle ◽  
Hollow Mesoporous Silica ◽  
Manganese Carbonyl ◽  
Co Gas

H2O2-triggered release of CO: an intratumoral H2O2-responsive nanomedicine is constructed by effectively encapsulating the hydrophilic manganese carbonyl prodrug into an advanced hollow mesoporous silica nanoparticle carrier to realize selective killing of tumor cells, exhibiting high in vitro and in vivo efficacies of CO gas therapy.

scholarly journals Synergistic Effect of Mesoporous Silica and Hydroxyapatite in Loaded Poly(DL-lactic-co-glycolic acid) Microspheres on the Regeneration of Bone Defects

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Shu He ◽  
Kai-Feng Lin ◽  
Jun-Jun Fan ◽  
Gang Hu ◽  
Xin Dong ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Synergistic Effect ◽  
Bone Regeneration ◽  
Glycolic Acid ◽  
Rabbit Model ◽  
Silica Nanoparticle ◽  
Bone Defects ◽  
Regeneration Of Bone

A microsphere composite made of poly(DL-lactic-co-glycolic acid) (PLGA), mesoporous silica nanoparticle (MSN), and nanohydroxyapatite (nHA) (PLGA-MSN/nHA) was prepared and evaluated as bone tissue engineering materials. The objective of this study was to investigate the synergistic effect of MSN/nHA on biocompatibility as well as its potential ability for bone formation. First, we found that this PLGA-MSN/nHA composite performed good characteristics on microstructure, mechanical strength, and wettability. By cell culture experiments, the adhesion and proliferation rate of the cells seeded on PLGA-MSN/nHA composite was higher than those of the controls and high levels of osteogenetic factors such as ALP and Runx-2 were detected by reverse transcriptase polymerase chain reaction. Finally, this PLGA-MSN/nHA composite was implanted into the femur bone defect in a rabbit model, and its ability to induce bone regeneration was observed by histological examinations. Twelve weeks after implantation, the bone defects had significantly more formation of mature bone and less residual materials than in the controls. These results demonstrate that this PLGA-MSN/nHA composite, introducing both MSN and nHA into PLGA microspheres, can improve the biocompatibility and osteoinductivity of compositein vitroandin vivoand had potential application in bone regeneration.

scholarly journals Activation of MAT2A-RIP1 signaling axis reprograms monocytes in gastric cancer

2021 ◽  
Vol 9 (2) ◽  
pp. e001364
Author(s):  
Yan Zhang ◽  
Hui Yang ◽  
Jun Zhao ◽  
Ping Wan ◽  
Ye Hu ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cell Metabolism ◽  
Vital Role ◽  
Methionine Metabolism ◽  
Promoter Regions ◽  
Normal Tissues ◽  
Methionine Cycle

BackgroundThe activation of tumor-associated macrophages (TAMs) facilitates the progression of gastric cancer (GC). Cell metabolism reprogramming has been shown to play a vital role in the polarization of TAMs. However, the role of methionine metabolism in function of TAMs remains to be explored.MethodsMonocytes/macrophages were isolated from peripheral blood, tumor tissues or normal tissues from healthy donors or patients with GC. The role of methionine metabolism in the activation of TAMs was evaluated with both in vivo analyses and in vitro experiments. Pharmacological inhibition of the methionine cycle and modulation of key metabolic genes was employed, where molecular and biological analyses were performed.ResultsTAMs have increased methionine cycle activity that are mainly attributed to elevated methionine adenosyltransferase II alpha (MAT2A) levels. MAT2A modulates the activation and maintenance of the phenotype of TAMs and mediates the upregulation of RIP1 by increasing the histone H3K4 methylation (H3K4me3) at its promoter regions.ConclusionsOur data cast light on a novel mechanism by which methionine metabolism regulates the anti-inflammatory functions of monocytes in GC. MAT2A might be a potential therapeutic target for cancer cells as well as TAMs in GC.

Development of mesoporous silica-based nanoprobes for optical bioimaging applications

2021 ◽  
Author(s):  
Bo Sun ◽  
Xu Zhen ◽  
Xiqun Jiang
Keyword(s):  
Mesoporous Silica ◽  
Fluorescence Imaging ◽  
Photoacoustic Imaging

This review mainly introduced the MSNs-based nanoprobes for in vivo bioimaging applications including fluorescence imaging and photoacoustic imaging.

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