scholarly journals Use of Adipose Stem Cells Against Hypertrophic Scarring or Keloid

2022 ◽  
Vol 9 ◽  
Author(s):  
Hongbo Chen ◽  
Kai Hou ◽  
Yiping Wu ◽  
Zeming Liu
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Hypertrophic Scar ◽  
Synergistic Action ◽  
Adipose Stem Cells ◽  
Hypertrophic Scars ◽  
Hypertrophic Scarring ◽  
Multi Stage ◽  
Keloid Formation ◽  
Multiple Cells

Hypertrophic scars or keloid form as part of the wound healing reaction process, and its formation mechanism is complex and diverse, involving multi-stage synergistic action of multiple cells and factors. Adipose stem cells (ASCs) have become an emerging approach for the treatment of many diseases, including hypertrophic scarring or keloid, owing to their various advantages and potential. Herein, we analyzed the molecular mechanism of hypertrophic scar or keloid formation and explored the role and prospects of stem cell therapy, in the treatment of this condition.

Current Advances in Hypertrophic Scar and Keloid Management

2021 ◽  
Author(s):  
Natasha Barone ◽  
Tyler Safran ◽  
Joshua Vorstenbosch ◽  
Peter Davison ◽  
Sabrina Cugno ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Wound Healing ◽  
Psychological Health ◽  
Hypertrophic Scar ◽  
Tissue Response ◽  
Fibroblast Proliferation ◽  
Hypertrophic Scars ◽  
Physical Status ◽  
Management Options

AbstractHypertrophic scars and keloids are caused by excessive tissue response to dermal injury due to local fibroblast proliferation and collagen overproduction. This response occurs because of pathologic wound healing due to dysregulation in the inflammatory, proliferative, and/or remodeling phase. Patients with hypertrophic scars or keloids report reduced quality of life, physical status, and psychological health. Hypertrophic scars or keloids will develop in 30 to 90% of individuals, and despite their prevalence, treatment remains a challenge. Of the treatments currently available for hypertrophic scars and keloids few have been adequately supported by studies with appropriate experimental design. Here, we aim to review the available literature to provide up-to-date information on the etiology, epidemiology, histology, pathophysiology, prevention, and management options available for the treatment of hypertrophic scars and keloids and highlight areas where further research is required.

scholarly journals Onion Extract

2020 ◽  
pp. 209-213
Author(s):  
Julian Poetschke ◽  
Gerd G. Gauglitz
Keyword(s):  
Wound Healing ◽  
Hypertrophic Scar ◽  
Healing Process ◽  
Surgical Techniques ◽  
Therapeutic Strategies ◽  
Study Data ◽  
Wound Healing Process ◽  
Objective Data ◽  
Onion Extract ◽  
Keloid Formation

AbstractMultiple studies on hypertrophic scar and keloid formation have led to a plethora of therapeutic strategies in order to prevent or attenuate keloid and hypertrophic scar formation. To date, preventing pathologic scarring remains undoubtedly more effective than treating it. Next to specific surgical techniques and an appropriate general aftercare of fresh wounds, a multitude of scar gels, creams, patches, and ointments are available and are being promoted for scarless wound healing. Next to silicone-based products, onion extract or cepalin has been highlighted as one potential anti-scarring agent over recent years. Based on several studies, onion extract alone or in combination with allantoin and heparin seems to alleviate the wound-healing process and appears beneficial for preventional application in fresh scars. The study data available, however, remains overall relatively poor and clearly objective data regarding this approach is widely missing.

scholarly journals Effect of THBS1 on the Biological Function of Hypertrophic Scar Fibroblasts

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
D. Jiang ◽  
B. Guo ◽  
F. Lin ◽  
Q. Hui ◽  
K. Tao
Keyword(s):  
Hypertrophic Scar ◽  
Wound Repair ◽  
Biological Function ◽  
Hypertrophic Scars ◽  
Hypertrophic Scarring ◽  
Skin Damage ◽  
Skin Collagen ◽  
And Migration ◽  
Tgf Β1

Hypertrophic scarring is a skin collagen disease that can occur following skin damage and is unlikely to heal or subside naturally. Since surgical treatment often worsens scarring, it is important to investigate the pathogenesis and prevention of hypertrophic scarring. Thrombospondin-1 (THBS1) is a matrix glycoprotein that can affect fibrosis by activating TGF-β1, which plays a key role in wound repair and tissue regeneration; therefore, we investigated the effects of THBS1 on the biological function of hypertrophic scar fibroblasts. THBS1 expression was measured in hypertrophic scars and adjacent tissues as well as normal fibroblasts, normal scar fibroblasts, and hypertrophic scar fibroblasts. In addition, THBS1 was overexpressed or silenced in hypertrophic scar fibroblasts to determine the effects of THBS1 on cell proliferation, apoptosis, and migration, as well as TGF-β1 expression. Finally, the role of THBS1 in hypertrophic scarring was confirmed in vivo using a mouse model. We found that THBS1 expression was increased in hypertrophic scar tissues and fibroblasts and promoted the growth and migration of hypertrophic scar fibroblasts as well as TGF-β1 expression. Interestingly, we found that si-THBS1 inhibited the occurrence and development of bleomycin-induced hypertrophic scars in vivo and downregulated TGF-β1 expression. Together, our findings suggest that THBS1 is abnormally expressed in hypertrophic scars and can induce the growth of hypertrophic scar fibroblasts by regulating TGF-β1. Consequently, THBS1 could be an ideal target for treating hypertrophic scarring.

Effectiveness of the adipose stem cells in burn wound healing: literature review

2021 ◽  
Author(s):  
Ahmad Oryan ◽  
Effat Alemzadeh ◽  
Esmat Alemzadeh ◽  
Maryam Barghi ◽  
Mohammad Zarei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Literature Review ◽  
Adipose Stem Cells ◽  
Burn Wound ◽  
Burn Wound Healing

Hairless Descendants of Mexican Hairless Dogs: An Experimental Model for Studying Hypertrophic Scars

2011 ◽  
Vol 15 (6) ◽  
pp. 329-339 ◽  
Author(s):  
Tohru Kimura
Keyword(s):  
Hypertrophic Scar ◽  
Inflammatory Cell ◽  
Scar Formation ◽  
Elastic Fibers ◽  
Tissue Formation ◽  
Hypertrophic Scars ◽  
Full Thickness ◽  
Hypertrophic Scarring ◽  
Granulation Tissue Formation ◽  
Clinical Observations

Background/Objective: In previous studies, the author noticed hypertrophic scar formation in hairless dogs. The purpose of this study was to evaluate both grossly and histopathologically hypertrophic scars in hairless dogs to explore any similarities with hypertrophic scars in humans and to introduce these dogs as an appropriate model for further investigation on hypertrophic scarring. Methods: Full-thickness wounds were made on the dorsolumbar skin of hairless dogs. Hypertrophic scarring was examined with three methods: clinical observations, dihydroxyphenylalanine (DOPA)-positive melanocytes, and skin histopathology. Results: Hairless dogs clinically developed the formation of hyperpigmented and hypertrophic scars that did not extend beyond the original wound margins. In hypertrophic scars of hairless dogs, the split epidermal sheet showed an increased number of DOPA-positive melanocytes with well-developed dendrites exhibiting activated melanocytes. There were very few DOPA-positive melanocytes in the repaired skin of haired dogs. Histopathologic examinations demonstrated that hypertrophic scars were fully reepithelialized and granulation tissue formation was accompanied by inflammatory cell infiltration. There was remodeling of thick collagens and fine elastic fibers in the course of hypertrophic scar formation. Conclusion: Experimental hypertrophic scars produced in hairless dogs have morphologic properties similar to those of human hypertrophic scars.

scholarly journals Combination of Lyophilized Adipose-Derived Stem Cells Concentrated Conditioned Medium and Polysaccharide Hydrogel in the Inhibition of Hypertrophic Scarring

2020 ◽  
Author(s):  
Chaoyu Zhang ◽  
Ting Wang ◽  
Li Zhang ◽  
Penghong Chen ◽  
Shijie Tang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Conditioned Medium ◽  
Hypertrophic Scar ◽  
Dose Group ◽  
Therapeutic Effects ◽  
Dependent Manner ◽  
Adipose Derived Stem Cells ◽  
Hypertrophic Scarring ◽  
Rabbit Ear ◽  
Polysaccharide Hydrogel

Abstract BackgroundMesenchymal stem cell-based acellular therapies have been widely exploited in managing hypertrophic scar. However, low maintenance dose and transitory therapeutic effects during topical medication remain a thorny issue. Herein, this study aimed to optimize the curative effect of adipose-derived stem cells conditioned medium (ADSC-CM) in the prevention of hypertrophic scarring. MethodsIn the present study, ADSC-CM was concentrated via the freeze-drying procedure. The efficacy of different dose groups (CM, CM5, CM10) was conducted on the proliferation, apoptosis, and α-smooth muscle actin (α-SMA) expression of human keloid fibroblasts (HKFs) in vitro. Incorporation of adipose-derived stem cells concentrated conditioned medium (ADSCC-CM) into polysaccharide hydrogel was investigated in rabbit ear, in vivo. Haematoxylin-eosin (H&E) and Masson's trichrome staining were performed for the evaluation of scar hyperplasia. ResultsWe noted that ADSCC-CM could downregulate the α-SMA expression of HKFs in a dose-dependent manner. In the rabbit ear model, the scar hyperplasia in the medium-dose group (CM5) and high-dose group (CM10) was inhibited with reduced scar elevation index (SEI) under 4 months of observation. It is noteworthy that the union of CM5 and polysaccharide hydrogel (CM5+H) yielded the best preventive effect on scar hyperplasia. Briefly, melanin, height, vascularity and pliability in the CM5+H group were better than those of the control group. Collagen was evenly distributed, and skin appendages could be regenerated.ConclusionsAltogether, ADSCC-CM can downregulate the expression of α-SMA due to its anti-fibrosis effect, and promote the rearrangement of collagen fibres, which is integral to scar precaution. The in situ cross bonding of ADSCC-CM and polysaccharide hydrogel could remarkably enhance the therapeutic outcomes in inhibiting scar proliferation. Hence, the alliance of ADSCC-CM and hydrogel may become a potential alternative in hypertrophic scar prophylaxis.

scholarly journals Hypoxia adipose stem cell-derived exosomes promote high-quality healing of diabetic wound involves activation of PI3K/Akt pathways

2021 ◽  
Author(s):  
Jie Wang ◽  
Hao Wu ◽  
Yixuan Peng ◽  
Yue Zhao ◽  
Youyou Qin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Molecular Mechanisms ◽  
High Throughput Sequencing ◽  
Transforming Growth Factor ◽  
Optimal Solution ◽  
Adipose Stem Cells ◽  
Diabetic Wound ◽  
Skin Wound Healing ◽  
Diabetic Wounds

Abstract Refractory diabetic wounds can cause persistent inflammation and delayed healing due to hypoxia. Currently, no optimal solution is available. Exosomes of adipose stem cells (ADSCs-exo) may promote skin wound healing, however, molecular mechanisms remains mysterious. We found significantly enhanced survival and proliferation of adipose stem cells after hypoxia induction compared to normoxia. Here, we aimed to investigate if hypoxic adipose stem cells exosomes (HypADSCs-exo) participate in hypoxia adaptability and accelerate diabetic wound healing. Based on high-throughput sequencing, 215 microRNAs (miRNAs) were upregulated and 369 miRNAs downregulated in HypADSCs-exo compared to ADSCs-exo. Up-regulated miR-21-3p, miR-126-5p, miR-31-5p whereas down-regulated gene miR-99b and miR-146-a correlated with wound healing. According to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), these miRNAs might regulate cell metabolism, differentiation and Transforming growth factor-β (TGF-β) function. Consistently, HpyADSCs-exo could promote diabetic wounds healing and inhibit inflammation through PI3K/AKT signaling pathway. Collectively, HpyADSCs-exo may potentially be applied in clinical therapy as an alternative strategy to improve wound healing.

IN VITRO MODEL OF A HYPERTROPHIC SCAR

2020 ◽  
Author(s):  
Валериан Сергеевич Шадрин ◽  
Петр Михайлович Кожин ◽  
Олеся Олеговна Шошина ◽  
Александр Леонидович Русанов
Keyword(s):  
Wound Healing ◽  
Hypertrophic Scar ◽  
In Vitro Model ◽  
World Health ◽  
Hypertrophic Scarring ◽  
Actual Problem ◽  
Vitro Model

Изучение нарушения ранозаживления, приводящего к развитию гиперпластических процессов, является актуальной задачей мирового здравоохранения. Для более детального изучения данной проблематики предложена новая in vitro модель, которая является более удобной для применения и при этом отражает признаки, характерные для гипертрофического рубцевания. The study of wound healing disorders leading to the development of hyperplastic processes is an actual problem of world health. A new in vitro model is proposed for a more detailed study of this problem. This model is more convenient for use and at the same time reflects the signs characteristic of hypertrophic scarring.

Sign in / Sign up

Export Citation Format

Share Document