scholarly journals DNA Condensation Triggered by the Synergistic Self-Assembly of Tetraphenylethylene-Viologen Aggregates and CT-DNA

2021 ◽  
Vol 9 ◽  
Author(s):  
Sajena Kanangat Saraswathi ◽  
Varsha Karunakaran ◽  
Kaustabh Kumar Maiti ◽  
Joshy Joseph
Keyword(s):  
Self Assembly ◽  
Fluorescence Emission ◽  
Dna Condensation ◽  
Condensation Process ◽  
Cellular Delivery ◽  
Groove Binding ◽  
Binding Interaction ◽  
Morphological Studies ◽  
Condensing Agents ◽  
Ct Dna

Development of small organic chromophores as DNA condensing agents, which explore supramolecular interactions and absorbance or fluorescence-based tracking of condensation and gene delivery processes, is in the initial stages. Herein, we report the synthesis and electrostatic/groove binding interaction–directed synergistic self-assembly of the aggregates of two viologen-functionalized tetraphenylethylene (TPE-V) molecules with CT-DNA and subsequent concentration-dependent DNA condensation process. TPE-V molecules differ in their chemical structure according to the number of viologen units. Photophysical and morphological studies have revealed the interaction of the aggregates of TPE-V in Tris buffer with CT-DNA, which transforms the fibrous network structure of CT-DNA to partially condensed beads-on-a-string-like arrangement with TPE-V aggregates as beads via electrostatic and groove binding interactions. Upon further increasing the concentration of TPE-V, the “beads-on-a-string”-type assembly of TPE-V/CT-DNA complex changes to completely condensed compact structures with 40–50 nm in diameter through the effective charge neutralization process. Enhancement in the melting temperature of CT-DNA, quenching of the fluorescence emission of ethidium bromide/CT-DNA complex, and the formation of induced CD signal in the presence of TPE-V molecules support the observed morphological changes and thereby verify the DNA condensation abilities of TPE-V molecules. Decrease in the hydrodynamic size, increase in the zeta potential value with the addition of TPE-V molecules to CT-DNA, failure of TPE-V/cucurbit(8)uril complex to condense CT-DNA, and the enhanced DNA condensation ability of TPE-V2 with two viologen units compared to TPE-V1 with a single viologen unit confirm the importance of positively charged viologen units in the DNA condensation process. Initial cytotoxicity analysis on A549 cancer and WI-38 normal cells revealed that these DNA condensing agents are non-toxic in nature and hence could be utilized in further cellular delivery studies.

scholarly journals Regulation of bi-color fluorescence changes of AIE supramolecular self-assembly gels by the interaction with Al3+ and energy transfer

2021 ◽  
Author(s):  
Xinxian Ma ◽  
Jinlong Yue ◽  
Bo Qiao ◽  
LIli Zhou ◽  
Yang Gao ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Self Assembly ◽  
Fluorescence Emission ◽  
Fluorescent Materials

Supramolecular fluorescent materials have attracted considerable attention in recent years since they endow specific and unique properties to materials. Nevertheless, the utilization of photo-responsive characteristics to modulate their fluorescence emission...

A New Approach to Fluorescent Chemosensor of Cu2+ Ion

2014 ◽  
Vol 513-517 ◽  
pp. 65-69
Author(s):  
Xiao Jun Hu ◽  
Xin Yan Hu ◽  
Zhi Zhang
Keyword(s):  
Aqueous Solution ◽  
Electron Transfer ◽  
Self Assembly ◽  
Ph Value ◽  
Sodium Dodecyl ◽  
Fluorescence Emission ◽  
Fluorescent Chemosensor ◽  
Dynamic Quenching ◽  
Transfer Effects ◽  
New Approach

According to the principle of dynamic quenching a new ON-OFF fluorescent chemosensor for Cu2+ions was designed, this chemosensor was composed of p-tert-butylthiacalix [arene (TCA),sodium dodecyl sulfate (SDS) and perylene through the form of self-assembly in aqueous solution. Addition of Cu2+ions could result in a quenching of the fluorescence emission of perylene inside micelles, which due to intramicellar complex-fluorophore electron-transfer or energy-transfer effects induced by the complexation of TCA with the Cu2+ions.The experimental results indicated that: Under the condition of TCA/perylene was 800/1, SDS concentration was 150mmol/L and pH value above 9, according to the fluorescence quenching ,within a certain range of the concentration of Cu2+ion can be linearly determined.

DNA Condensation by PAMAM Dendrimers:  Self-Assembly Characteristics and Effect on Transcription†

Biochemistry ◽  
2008 ◽  
Vol 47 (6) ◽  
pp. 1732-1740 ◽  
Author(s):  
Kristina Fant ◽  
Elin K. Esbjörner ◽  
Per Lincoln ◽  
Bengt Nordén
Keyword(s):  
Self Assembly ◽  
Pamam Dendrimers ◽  
Dna Condensation

A new twofold interpenetrated two-dimensional cadmium(II) coordination polymer constructed from 2,2′-(benzene-1,4-dicarboxamido)dipropionate

2018 ◽  
Vol 74 (11) ◽  
pp. 1434-1439
Author(s):  
Hong-Tao Zhang ◽  
Xiao-Long Wang
Keyword(s):  
Coordination Polymer ◽  
Coordination Polymers ◽  
Self Assembly ◽  
Fluorescence Emission ◽  
Functional Materials ◽  
Two Dimensional ◽  
X Ray Diffraction ◽  
X Ray ◽  
Carboxylate Groups ◽  
Potential Applications

In recent years, much initial interest and enthusiasm has focused on the self-assembly of coordination polymers due to the aesthetics of their crystalline architectures and their potential applications as new functional materials. As part of an exploration of chiral coordination polymers, a new twofold interpenetrated two-dimensional (2D) coordination polymer, namely, poly[[tetraaquabis[μ3-(2R,2′R)-2,2′-(benzene-1,4-dicarboxamido)dipropionato-κ5 O,O′:O′′,O′′′:O′′]dicadmium(II)] trihydrate], {[Cd2(C14H14N2O6)2(H2O)4]·3H2O} n , has been synthesized by the reaction of Cd(CH3COO)2·2H2O with the designed ligand (2R,2′R)-2,2′-(benzene-1,4-dicarboxamido)dipropionic acid (H2 L). The compound has been structurally characterized by elemental analysis, IR spectroscopy, powder X-ray diffraction and single-crystal X-ray diffraction analysis. In the crystal structure, each CdII cation binds to three carboxylate groups from two crystallographically independent L 2− dianions. Four carboxylate groups link two crystallographically independent cadmium cations into a 4,4-connected secondary building unit (SBU). The resulting SBUs are extended into a two-dimensional folding sheet via the terephthalamide moiety of the ligand as a spacer, which can be simplified as a (4,4)-connected 4,4L15 net with the point symbol (3.53.62)(32.52.62). In the lattice, two independent folding sheets interpenetrate each other to yield a double-sheet layer. The resulting 2D layers pack in parallel arrays through intermolecular hydrogen bonds and interlayer π–π interactions. The thermal stability and photoluminescence properties of the title compound have been investigated and it exhibits an enhanced fluorescence emission and a longer lifetime compared with free H2 L.

scholarly journals Stability of Chlorophyll a Monomer Incorporated into Cremophor EL Nano-Micelles under Dark and Moderate Light Conditions

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5059
Author(s):  
Ewa Janik-Zabrotowicz ◽  
Marta Arczewska ◽  
Patrycja Prochniewicz ◽  
Izabela Świetlicka ◽  
Konrad Terpiłowski
Keyword(s):  
Chlorophyll A ◽  
Self Assembly ◽  
Molecular Spectroscopy ◽  
Fluorescence Emission ◽  
Molecular Organization ◽  
Cremophor El ◽  
Light Conditions ◽  
Fluorescence Emission Spectra ◽  
Pigment Stability ◽  
The Stability

In this paper, stability of chlorophyll a monomers encapsulated into the Cremophor EL nano-micelles was studied under dark and moderate light conditions, typical of a room with natural or artificial lighting, in the presence of oxygen. The pigment stability against visible light was determined using the dynamic light scattering and molecular spectroscopy (UV-Vis absorption and stationary fluorescence) methods. Chlorophyll a, at the molar concentration of 10−5 M, was dissolved in the 5 wt% Cremophor emulsion for comparison in the ethanolic solution. The stability of such a self-assembly pigment–detergent nano-system is important in the light of its application on the commercial-scale. The presented results indicate the high stability of the pigment monomeric molecular organization in the nano-emulsion. During the storage in the dark, the half-lifetime was calculated as about 7 months. Additionally, based on the shape of absorption and fluorescence emission spectra, chlorophyll aggregation in the Cremophor EL aqueous solution along with the time was excluded. Moreover, the average size of detergent micelles as chlorophyll carriers was not affected after 70 days of the nano-system storage. Pigment stability against the moderate white light (0.1 mW) did not differ significantly from storage conditions in the dark. The photooxidation products, detected by occurrence of new absorption and fluorescence emission bands, was estimated on the negligible level. The stability of such a self-assembly pigment–detergent nano-system would potentially broaden the field of chlorophyll a (chl a) application in the food industry, medicine or artificial photosynthesis models.

scholarly journals Dynamic self-assembly of DNA minor groove-binding ligand DB921 into nanotubes triggered by an alkali halide

Nanoscale ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 5550-5558 ◽  
Author(s):  
R. Mizuta ◽  
J. M. Devos ◽  
J. Webster ◽  
W. L. Ling ◽  
T. Narayanan ◽  
...  
Keyword(s):  
Self Assembly ◽  
Alkali Halide ◽  
Minor Groove ◽  
Minor Groove Binding ◽  
Groove Binding ◽  
Dna Minor Groove ◽  
Self Assembling ◽  
System P

We describe the preparation of a novel self-assembling supramolecular nanotube system.

Self-assembly and fluorescence emission of UV-responsive azobenzene-containing helical poly(phenyl isocyanide) copolymers

2020 ◽  
Vol 11 (37) ◽  
pp. 6029-6036 ◽  
Author(s):  
Qian-Wei Li ◽  
Yi-Xu Su ◽  
Hui Zou ◽  
Yong-Yuan Chen ◽  
Li Zhou ◽  
...  
Keyword(s):  
Self Assembly ◽  
Fluorescence Emission ◽  
Fluorescent Properties

UV-responsive azobenzene-containing helical copolymers were obtained, and their self-assembly and fluorescent properties were investigated.

scholarly journals Self-Assembly and Soft Material Preparation of Binary Organogels via Aminobenzimidazole/Benzothiazole and Acids with Different Alkyl Substituent Chains

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Tifeng Jiao ◽  
Keren Ma ◽  
Xihai Shen ◽  
Qingrui Zhang ◽  
Xiujin Li ◽  
...  
Keyword(s):  
Organic Solvents ◽  
Self Assembly ◽  
Alkyl Substituent ◽  
Soft Materials ◽  
Spectral Studies ◽  
Hydrophobic Force ◽  
Benzothiazole Derivatives ◽  
Morphological Studies ◽  
Gelation Behavior ◽  
Potential Applications

The gelation behaviors of binary organogels composed of aminobenzimidazole/benzothiazole derivatives and benzoic acid with single-/multialkyl substituent chain in various organic solvents were designed and investigated. Their gelation behaviors in 20 solvents were tested as new binary organic gelators. This showed that the number and length of alkyl substituent chains and benzimidazole/benzothiazole segment have played a crucial role in the gelation behavior of all gelator mixtures in various organic solvents. More alkyl chains in molecular skeletons in present gelators are favorable for the gelation of organic solvents. The length of alkyl substituent chains has also played an important role in changing the gelation behaviors and assembly states. Morphological studies revealed that the gelator molecules self-assemble into different aggregates from wrinkle, lamella, belt, to fiber with change of solvents. Spectral studies indicated that there existed different H-bond formation and hydrophobic force, depending on benzimidazole/benzothiazole segment and alkyl substituent chains in molecular skeletons. The prepared nanostructured materials have wide perspectives and many potential applications in nanoscience and material fields due to their scientific values. The present work may also give new clues for designing new binary organogelators and soft materials.

scholarly journals DNA Interaction Studies of a New Platinum(II) Complex Containing Different Aromatic Dinitrogen Ligands

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Nahid Shahabadi ◽  
Somaye Mohammadi ◽  
Robabeh Alizadeh
Keyword(s):  
Absorption Band ◽  
Bathochromic Shift ◽  
Binding Mode ◽  
Dna Interaction ◽  
Quenching Mechanism ◽  
Binding Interaction ◽  
Fluorescence Studies ◽  
Cyclic Voltametry ◽  
Calf Thymus ◽  
Ct Dna

A new mononuclear Pt(II) complex, [Pt(DMP)(DIP)]Cl2.H2O, in which DMP is 4,4-dimethyl-2,2-bipyridine and DIP is 4,7-diphenyl-1,10-phenantroline, has been synthesized and characterized by physicochemical and spectroscopic methods. The binding interaction of this complex with calf thymus DNA (CT-DNA) was investigated using fluorimetry, spectrophotometry, circular dichroism, viscosimetry and cyclic voltametry (CV). UV-VIS spectrum showed 4 nm bathochromic shift of the absorption band at 280 nm along with significant hypochromicity for the absorption band of the complex. The intrnisic binding constant is more in keeping with intercalators and suggests this binding mode. The viscosity measurements showed that the complex-DNA interaction can be hydrophobic and confirm intercalation. Moreover, the complex induced detectable changes in the CD spectrum of CT-DNA. The fluorescence studies revealed that the probable quenching mechanism of fluorescence of the complex by CT-DNA is static quenching. The thermodynamic parameters ( and ) showed that main interaction with hydrogenic forces occurred that is intercalation mode. Also, CV results confirm this mode because, with increasing the CT-DNA concentration, shift to higher potential was observed.

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