scholarly journals DNA Interaction Studies of a New Platinum(II) Complex Containing Different Aromatic Dinitrogen Ligands

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Nahid Shahabadi ◽  
Somaye Mohammadi ◽  
Robabeh Alizadeh
Keyword(s):  
Absorption Band ◽  
Bathochromic Shift ◽  
Binding Mode ◽  
Dna Interaction ◽  
Quenching Mechanism ◽  
Binding Interaction ◽  
Fluorescence Studies ◽  
Cyclic Voltametry ◽  
Calf Thymus ◽  
Ct Dna

A new mononuclear Pt(II) complex, [Pt(DMP)(DIP)]Cl2.H2O, in which DMP is 4,4-dimethyl-2,2-bipyridine and DIP is 4,7-diphenyl-1,10-phenantroline, has been synthesized and characterized by physicochemical and spectroscopic methods. The binding interaction of this complex with calf thymus DNA (CT-DNA) was investigated using fluorimetry, spectrophotometry, circular dichroism, viscosimetry and cyclic voltametry (CV). UV-VIS spectrum showed 4 nm bathochromic shift of the absorption band at 280 nm along with significant hypochromicity for the absorption band of the complex. The intrnisic binding constant is more in keeping with intercalators and suggests this binding mode. The viscosity measurements showed that the complex-DNA interaction can be hydrophobic and confirm intercalation. Moreover, the complex induced detectable changes in the CD spectrum of CT-DNA. The fluorescence studies revealed that the probable quenching mechanism of fluorescence of the complex by CT-DNA is static quenching. The thermodynamic parameters ( and ) showed that main interaction with hydrogenic forces occurred that is intercalation mode. Also, CV results confirm this mode because, with increasing the CT-DNA concentration, shift to higher potential was observed.

scholarly journals Synthesis, DNA interaction and antibacterial activities of La(III) and Gd(III) complexes of Schiff base derived from o-vanillin and lysine

2009 ◽  
Vol 7 (1) ◽  
pp. 105-110 ◽  
Author(s):  
Jian-Ping Cheng ◽  
Qiu-Yue Lin ◽  
Hu Rui-Ding ◽  
Wen-Zhong Zhu ◽  
Hua-Qiong Li ◽  
...  
Keyword(s):  
Schiff Base ◽  
Binding Mode ◽  
Dna Interaction ◽  
Antibacterial Activities ◽  
Azomethine Nitrogen ◽  
Moderate Activity ◽  
Phenolic Oxygen ◽  
Calf Thymus ◽  
Ct Dna ◽  
Remarkable Reduction

AbstractTwo novel complexes, [La(HL)(H2O)2NO3] · NO3 · H2O and [Gd(HL)(H2O)2NO3] · NO3 · H2O, where HL is a Schiff base derived from o-vanillin and lysine, have been synthesized and characterized by elemental analysis, conductivity measurements, IR, 1H NMR and thermogravimetric analyses (TGA). The Schiff base ligand behaves as a tetradentate, coordinating through azomethine nitrogen, phenolic oxygen and two carboxylic oxygen atoms. The interaction of these complexes with calf thymus DNA (CT-DNA) was also investigated by spectrometric titration and viscometric measurements. The faint hypochromism of the complexes in the absorption spectra, the remarkable reduction of fluorescence intensity of ethidium bromide (EB) bound DNA, together with a small decrease in the viscosity of the DNA suggest that a partial intercalation may be the preferred binding mode between these two complexes and DNA. The antibacterial activity testing revealed that the complexes and their precursor Schiff base show a weak to moderate activity against Bacillus subtilis, Staphylococcus aureus and Escherichia coli.

scholarly journals Study of the Binding Interaction between Wortmannin and Calf Thymus DNA: Multispectroscopic and Molecular Docking Studies

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Shiva Mehran ◽  
Yousef Rasmi ◽  
Hamid Reza Karamdel ◽  
Ramin Hossinzadeh ◽  
Zafar Gholinejad
Keyword(s):  
Molecular Docking ◽  
Hydrogen Bonds ◽  
In Silico ◽  
Uv Spectroscopy ◽  
Biological Effects ◽  
Dna Interaction ◽  
Calf Thymus Dna ◽  
Binding Interaction ◽  
Calf Thymus ◽  
Ct Dna

Introduction. Wortmannin (WTN) is a steroid metabolite that inhibits phosphatidylinositol 3-kinase and other signaling pathways. Structurally, the WTN consists of a cyclopentanophenanthrene-like structure with several oxygen-rich moieties which have the potential to interact with deoxyribonucleic acid (DNA) molecules. Methods. We aim to evaluate the WTN and calf thymus DNA (ct-DNA) interaction with molecular docking using the AutoDock 4.2 software. UV and fluorescence spectroscopy and viscosity techniques were performed to confirm the in silico analysis. Results. Molecular docking showed that the WTN interacted with ct-DNA via hydrogen bonds at guanine-rich sequences. The number of hydrogen bonds between the WTN and DNA was 1-2 bonds (average 1.2) per WTN molecule. The in silico binding constant was 2 × 103 M−1. UV spectroscopy showed that the WTN induced a hyperchromic feature without wavelength shifting. The WTN and DNA interaction led to quenching of DNA-emitted fluorescence. The different concentrations of WTN had no effect on DNA viscosity. Taken together, our results demonstrated WTN interacts with DNA in the nonintercalating mode, which is considered as a new mechanism of action. Conclusion. These results suggest that the WTN may exert its biological effects, at least in part, via interaction with DNA.

scholarly journals DNA Interaction and DNA Cleavage Studies of a New Platinum(II) Complex Containing Aliphatic and Aromatic Dinitrogen Ligands

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Nahid Shahabadi ◽  
Soheila Kashanian ◽  
Maryam Mahdavi ◽  
Noorkaram Sourinejad
Keyword(s):  
Dna Cleavage ◽  
Helical Structure ◽  
Dna Interaction ◽  
Van Der Waals Interactions ◽  
Double Helical Structure ◽  
Intercalative Mode ◽  
Physico Chemical ◽  
Calf Thymus ◽  
Enthalpy And Entropy Changes ◽  
Ct Dna

A new Pt(II) complex, [Pt(DIP)(LL)](NO3)2(in which DIP is 4,7-diphenyl-1,10-phenanthroline and LL is the aliphatic dinitrogen ligand,N,N-dimethyl-trimethylenediamine), was synthesized and characterized using different physico-chemical methods. The interaction of this complex with calf thymus DNA (CT-DNA) was investigated by absorption, emission, circular dichroism (CD), and viscosity measurements. The complex binds to CT-DNA in an intercalative mode. The calculated binding constant,Kb, was  M−1. The enthalpy and entropy changes of the reaction between the complex and CT-DNA showed that the van der Waals interactions and hydrogen bonds are the main forces in the interaction with CT-DNA. In addition, CD study showed that phenanthroline ligand insert between the base pair stack of double helical structure of DNA. It is remarkable that this complex has the ability to cleave the supercoiled plasmid.

scholarly journals DNA binding and photo-induced DNA cleavage activity of Elsinochrome A in visible light

2011 ◽  
Vol 26 (4-5) ◽  
pp. 289-296
Author(s):  
Fei Ma ◽  
Jing Tang ◽  
Chao Yang ◽  
Yu-Ying Feng ◽  
Shao-Hua Wei ◽  
...  
Keyword(s):  
Visible Light ◽  
Dna Cleavage ◽  
Fluorescence Spectra ◽  
Binding Mode ◽  
Strong Force ◽  
Dna Cleavage Activity ◽  
Calf Thymus ◽  
Electrostatic Binding ◽  
Ct Dna ◽  
Elsinochrome A

The interaction of Elsinochrome A (EA) with calf thymus DNA (CT-DNA) has been investigated by UV-vis spectra and fluorescence spectra. The results show that EA can bind with CT-DNA and binding sites are destroyed after irradiation by visible light, which indicates that EA is a promising candidate for photodynamic therapy. In addition, the binding mechanism is studied using fluorescence quenching test and ethidium bromide (EB) replace assay experiments. The results suggest that EA and CT-DNA are binding with a strong force and the major binding mode of EA with DNA could be the electrostatic binding.

scholarly journals Interaction of Duloxetine Hydrochloride with Deoxyribonucleic Acid Measured by Fluorescence Spectroscopy

2016 ◽  
Vol 14 (2) ◽  
pp. 199-206
Author(s):  
Raznin Akter Joly ◽  
Md Reazul Islam ◽  
Sonia Sultana ◽  
Asma Rahman ◽  
Md Zakir Sultan ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Deoxyribonucleic Acid ◽  
Antidepressant Drug ◽  
Living Organism ◽  
Binding Mode ◽  
Duloxetine Hydrochloride ◽  
Calf Thymus ◽  
Binding Forces ◽  
Ct Dna ◽  
Hypochromic Effect

Interactions with many clinically active therapeutic agents with DNA are well studied and it is necessary to decipher the structure of DNA and to investigate the pathological implications of those molecules in living organism. This study investigated the interaction of antidepressant drug Duloxetine-hydrochloride (DLX) with calf thymus DNA (ct-DNA). The interaction of DLX with ct-DNA was studied employing fluorescence spectroscopy. Hypochromic effect was found in the absorption spectra of duloxetine, and its wavelength had no shift in the presence of DNA indicating external binding mode of duloxetine to DNA. Fluorescence spectroscopic results showed the quenching of fluorescence intensity of DLX in presence of DNA indicating the interaction between DLX and DNA. Hydrophobic interaction and hydrogen bonding played the dominating role in DLX-DNA binding and binding forces also indicate the binding site of duloxetine to be at the minor groove of DNA.Dhaka Univ. J. Pharm. Sci. 14(2): 199-206, 2015 (December)

N-Methylpyridylporphyrin tailed with folate conjugate as a potential lysosomal-targeted photosensitizer: Synthesis, DNA interaction, singlet oxygen and subcellular localization

2019 ◽  
Vol 23 (06) ◽  
pp. 679-684
Author(s):  
Yi-Mei Zhao ◽  
Qian-Qian Lu ◽  
Si Yao ◽  
Hui-Fang Su ◽  
Hong-Jian Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Fluorescence Spectra ◽  
Binding Constants ◽  
Binding Mode ◽  
Dna Interaction ◽  
H Nmr ◽  
Calf Thymus ◽  
Folate Conjugate ◽  
Tumor Drugs

In recent years, great interest has been focused on the use of photosensitizers (PS) for photodynamic therapy (PDT) as safe and effective anti-tumor drugs. As a good lysosomal-targeted drug, folic acid (FA) is highly interesting as well. [Formula: see text]-methylpyridylporphyrin tailed with folate conjugate (Me-Por-FA) was newly designed and synthesized and the structure was confirmed by UV-vis, IR, 1H NMR, MS and elemental analysis. The interaction of this porphyrin with calf thymus DNA was the intercalative binding mode, which was confirmed by ultraviolet and fluorescence spectra, and the binding constants [Formula: see text] was 6.24 × 104 L/mol. The singlet oxygen (1O[Formula: see text] generated by Me-Por-FA was determined by 1, 3-diphenylisobenzofuran (DPBF) method using tetrapyridylporphyrin (H[Formula: see text]TMPyP) as a comparison with the following order: H2TMPyP > Me-Por-FA. Stained with LysoTracker[Formula: see text] Green DND-26, Me-Por-FA was mainly distributed over the lysosomes during 4 h, but H[Formula: see text]TMPyP was not. This suggests that Me-Por-FA could be developed as a targeted photosensitizer for precise photodynamic therapy.

scholarly journals Determination of Nucleic Acids Using Fluorescence Probe Sensitized by Microemulsion

2006 ◽  
Vol 89 (6) ◽  
pp. 1609-1616
Author(s):  
Qin Wei ◽  
Hui Zhang ◽  
Bin Du ◽  
Caihong Duan
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Fluorescence Probe ◽  
High Sensitivity ◽  
Binding Mode ◽  
Sperm Dna ◽  
Calf Thymus ◽  
Triton X ◽  
Ct Dna

Abstract Because the fluorescence of azur A can be quenched by adding nucleic acid, a sensitive fluorometric method for determination of nucleic acids at nanogram levels was established. Using optimal conditions, the calibration curves were linear in the range of 06.0 μg/mL for calf thymus deoxyribonucleic acid (ct DNA) and 07.0 μg/mL for herring sperm DNA (hs DNA). The limits of determination were 3.5 and 3.8 ng/mL, respectively, which shows the high sensitivity of this method. Triton X-100 microemulsion was applied as a sensitive media to enhance the sensitivity. The binding mode concerning the interactions of azur A with nucleic acids was also studied and the association constant with different binding numbers was obtained. The method has been applied to the determination of nucleic acid in both synthetic and real samples, such as cauliflower and pork liver, with satisfactory results.

Surfactant Complex Binding to DNA Interaction Study: Controlling Hydrophobicity in β-Cyclodextrin–DNA Binding Reactions

2020 ◽  
pp. 318-332
Author(s):  
Nagaraj Karuppiah ◽  
◽  
Muthukumaran Pakkirisamy ◽  
Gunasekaran Gladwin ◽  
Keyword(s):  
Dna Interaction ◽  
Spectroscopic Studies ◽  
Calf Thymus Dna ◽  
Aliphatic Chain ◽  
Groove Binding ◽  
Base Pairs ◽  
Surfactant Complex ◽  
Calf Thymus ◽  
Frame Work ◽  
Ct Dna

The interaction of cis-[Co(phen)2(TA)2](ClO4)3, a cationic surfactant complex (phen = 1-10 phenanthroline, TA= Tetradecylamine), with calf thymus DNA has been studied by physici-chemical techniques. The spectroscopic studies together with cyclic voltammetry and viscosity experiments support that the surfactant-cobalt(III) complex binds to calf thymus DNA (CT DNA) by intercalation through the aliphatic chain present in the complex into the base pairs of DNA. The presence of phenanthroline ligand with larger -frame work may also enhance intercalation. Besides the effect of binding of surfactant cobalt(III) complex to DNA in presence of -cyclodextrin has also studied. In presence of -cyclodextrin the binding occur through surface and (or) groove binding. The complex was investigated as one of the potential

Spectroscopic and viscometric elucidation of the interaction between a potential chloride channel blocker and calf-thymus DNA: the effect of medium ionic strength on the binding mode

2015 ◽  
Vol 17 (1) ◽  
pp. 483-492 ◽  
Author(s):  
Aniruddha Ganguly ◽  
Soumen Ghosh ◽  
Nikhil Guchhait
Keyword(s):  
Ionic Strength ◽  
Chloride Channel ◽  
Channel Blocker ◽  
Binding Mode ◽  
Calf Thymus Dna ◽  
Binding Interaction ◽  
Detailed Characterization ◽  
Calf Thymus ◽  
Chloride Channel Blocker

The present study demonstrates a detailed characterization of the binding interaction of a potential chloride channel blocker 9-methyl anthroate (9-MA) with calf-thymus DNA.

scholarly journals Synthesis, Characterization, Photoluminescence, Molecular Docking and Bioactivity of Zinc (II) Compounds Based on Different Substituents

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3459
Author(s):  
Rongping Liu ◽  
Hao Yan ◽  
Jinzhang Jiang ◽  
Jiahe Li ◽  
Xing Liang ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Binding Mode ◽  
Cytotoxicity Test ◽  
X Ray Diffraction ◽  
Quenching Mechanism ◽  
X Ray ◽  
Ft Ir ◽  
Dna Fragment ◽  
Intercalative Binding ◽  
Ct Dna

Six new zinc(II) complexes were prepared by the reaction of ZnBr2 or ZnI2 with 4′-(substituted-phenyl)-2,2′:6′,2′′-terpyridine compounds, bearing p-methylsulfonyl (L1), p-methoxy (L2) and p-methyl (L3), which were characterized by elemental analysis, FT-IR, NMR and single crystal X-ray diffraction. The antiproliferative properties against Eca-109, A549 and Bel-7402 cell lines and the cytotoxicity test on RAW-264.7 of these compounds were monitored using a CCK-8 assay, and the studies indicate that the complexes show higher antiproliferative activities than cisplatin. The interactions of these complexes with CT-DNA and proteins (BSA) were studied by UV-Vis, circular dichroism (CD) and fluorescent spectroscopy, respectively. The results indicate that the interaction of these zinc(II) complexes with CT-DNA is achieved through intercalative binding, and their strong binding affinity to BSA is fulfilled through a static quenching mechanism. The simulation of the complexes with the CT-DNA fragment and BSA was studied by using molecular docking software. It further validates that the complexes interact with DNA through intercalative binding mode and that they have a strong interaction with BSA.

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