scholarly journals A Pro-Inflammatory Gut Microbiome Characterizes SARS-CoV-2 Infected Patients and a Reduction in the Connectivity of an Anti-Inflammatory Bacterial Network Associates With Severe COVID-19

Author(s):  
Johanna Reinold ◽  
Farnoush Farahpour ◽  
Christian Fehring ◽  
Sebastian Dolff ◽  
Margarethe Konik ◽  
...  

The gut microbiota contributes to maintaining human health and regulating immune responses. Severe COVID-19 illness is associated with a dysregulated pro-inflammatory immune response. The effect of SARS-CoV-2 on altering the gut microbiome and the relevance of the gut microbiome on COVID-19 severity needs to be clarified. In this prospective study, we analyzed the gut microbiome of 212 patients of a tertiary care hospital (117 patients infected with SARS-CoV-2 and 95 SARS-CoV-2 negative patients) using 16S rRNA gene sequencing of the V3-V4 region. Inflammatory markers and immune cells were quantified from blood. The gut microbiome in SARS-CoV-2 infected patients was characterized by a lower bacterial richness and distinct differences in the gut microbiome composition, including an enrichment of the phyla Proteobacteria and Bacteroidetes and a decrease of Actinobacteria compared to SARS-CoV-2 negative patients. The relative abundance of several genera including Bifidobacterium, Streptococcus and Collinsella was lower in SARS-CoV-2 positive patients while the abundance of Bacteroides and Enterobacteriaceae was increased. Higher pro-inflammatory blood markers and a lower CD8+ T cell number characterized patients with severe COVID-19 illness. The gut microbiome of patients with severe/critical COVID-19 exhibited a lower abundance of butyrate-producing genera Faecalibacterium and Roseburia and a reduction in the connectivity of a distinct network of anti-inflammatory genera that was observed in patients with mild COVID-19 illness and in SARS-CoV-2 negative patients. Dysbiosis of the gut microbiome associated with a pro-inflammatory signature may contribute to the hyperinflammatory immune response characterizing severe COVID-19 illness.

2018 ◽  
Vol 36 (3) ◽  
pp. 397-400
Author(s):  
Praveena Basireddy ◽  
Surekha Avileli ◽  
Nagajyothi Beldono ◽  
Swarna Latha Gundela

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0182899 ◽  
Author(s):  
Roberto Rosales-Reyes ◽  
Catalina Gayosso-Vázquez ◽  
José Luis Fernández-Vázquez ◽  
Ma Dolores Jarillo-Quijada ◽  
César Rivera-Benítez ◽  
...  

2021 ◽  
Vol 59 (243) ◽  
pp. 1116-1119
Author(s):  
Aashish Bhattarai ◽  
Anna Acharya ◽  
Krishna Raj Khanal

Introduction: Non-steroidal anti-inflammatory drugs are major drugs in treatment of pain and inflammation of different orthopedic conditions. There are different classes of non-steroidal anti-Inflammatory Drugs based on their selectivity to cyclooxygenase enzyme which has significant differences in safety profile. This study aims to determine the prevalence of non-steroidal anti-inflammatory drugs prescription in the orthopaedic outpatient department of a tertiary care hospital. Methods: This was a descriptive cross-sectional study conducted among the patients in the orthopedic outpatient department of tertiary care hospital from December 2020 to March 2021. Ethical approval was taken from the Institutional Review Committee of the college (Ref: 0311202007). Convenient sampling was done. A structured proforma was used with consent. The data were analyzed with Social Statistical Package for the Social Sciences version 20. Point estimate at 95% Confidence Interval was done and frequency and percentage were calculated. Results: Out of 140 orthopaedic out patient department prescriptions screened, 118 (84.28%) (78.25-90.30 at 95% Confidence Interval) prescriptions included non-steroidal anti-inflammatory drugs. Aceclofenac 76 (64.4%) was the most prescribed non-steroidal anti-inflammatory drug. Conclusions: Aceclofenac was the most preferred agent for the treatment in the department of orthopedics. The most common proton pump inhibitor used alone with non-steroidal anti-inflammatory drugs was rabeprazole.


Sign in / Sign up

Export Citation Format

Share Document