scholarly journals Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein

2021 ◽  
Vol 12 ◽  
Author(s):  
Ge Li ◽  
Aiping Wang ◽  
Yumei Chen ◽  
Yaning Sun ◽  
Yongkun Du ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Colloidal Gold ◽  
Global Economy ◽  
Chinese Hamster ◽  
Influenza Viruses ◽  
Spike Protein ◽  
Disease Spread ◽  
Ovary Cell ◽  
Immunochromatographic Strip ◽  
Specific Reactions

The outbreak and worldwide pandemic of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have a significant impact on global economy and human health. In order to reduce the disease spread, 16 monoclonal antibodies (McAbs) again SARS-CoV-2 were generated by immunized mice with the spike protein receptor binding domain (RBD), which was expressed in Chinese hamster ovary cell (CHO). A colloidal gold-based immunochromatographic strip was developed with two McAbs to detect SARS-CoV-2 spike protein, which can play a potential role in monitoring vaccine quality. The strip is highly specific, detecting only SARS-CoV-2 spike protein, and does not show any non-specific reactions with syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV) and other coronavirus and influenza viruses. The strip detected subunit vaccine in our laboratory with a detection limit of spike protein of 62.5 ng/mL. This strip provides an effective method in monitoring vaccine quality by detecting the antigen content of spike protein.

scholarly journals COVseq is a cost-effective workflow for mass-scale SARS-CoV-2 genomic surveillance

2021 ◽  
Author(s):  
Michele Simonetti ◽  
Ning Zhang ◽  
Luuk Harbers ◽  
Maria Grazia Milia ◽  
Thi Thu Huong Nguyen ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Protein A ◽  
Cost Effective ◽  
Mass Scale ◽  
Influenza Viruses ◽  
Spike Protein ◽  
Genome Coverage ◽  
Effective Manner ◽  
Geographical Regions ◽  
Different Populations

Abstract Genomic surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical to monitor the spread and evolution of the virus across different populations, geographical regions and species. Here, we present a streamlined workflow—COVseq—based on the CUTseq method that we previously described, which can be used to generate highly multiplexed sequencing libraries compatible with Illumina platforms, from hundreds of SARS-CoV-2 samples in parallel, in a rapid and cost-effective manner. We validated COVseq on RNA extracted from the supernatant of a SARS-CoV-2 culture as well as from 85 left-over samples from nasopharyngeal swabs, demonstrating the ability of COVseq to achieve almost complete genome coverage, including the S region encoding the spike protein. A cost analysis showed that COVseq could be used to sequence thousands of samples per week at less than 20 USD per sample. COVseq is a versatile and scalable method that can be readily applied for genomic surveillance of the ongoing pandemic and easily adapted to other pathogens such as influenza viruses.

Development of an antigen-ELISA and a colloidal gold–based immunochromatographic strip based on monoclonal antibodies for detection of avian influenza A(H5) viruses

2021 ◽  
pp. 104063872110275
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Linfang Cheng ◽  
Hangping Yao ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Disease Virus ◽  
Colloidal Gold ◽  
Rapid Detection ◽  
Influenza A ◽  
Field Investigation ◽  
Cross Reactivity ◽  
Detection Methods ◽  
Immunochromatographic Strip ◽  
Antigen Elisa

Avian influenza A(H5) viruses (avian IAVs) pose a major threat to the economy and public health. We developed an antigen-ELISA (ag-ELISA) and a colloidal gold–based immunochromatographic strip for the rapid detection of avian A(H5) viruses. Both detection methods displayed no cross-reactivity with other viruses (e.g., other avian IAVs, infectious bursal disease virus, Newcastle disease virus, infectious bronchitis virus, avian paramyxovirus). The ag-ELISA was sensitive down to 0.5 hemagglutinin (HA) units/100 µL of avian A(H5) viruses and 7.5 ng/mL of purified H5 HA proteins. The immunochromatographic strip was sensitive down to 1 HA unit/100 µL of avian A(H5) viruses. Both detection methods exhibited good reproducibility with CVs < 10%. For 200 random poultry samples, the sensitivity and specificity of the ag-ELISA were 92.6% and 98.8%, respectively, and for test strips were 88.9% and 98.3%, respectively. Both detection methods displayed high specificity, sensitivity, and stability, making them suitable for rapid detection and field investigation of avian A(H5) viruses.

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