scholarly journals Perspective for a New Bioinspired Permanent Adhesive for dry Conditions - Insights in the Glue Producing Japanese art of Defence System of the Oita Salamander Hynobius dunni

2021 ◽  
Vol 7 ◽  
Author(s):  
Janek von Byern ◽  
Robert Farkaš ◽  
Daniel Steinort ◽  
Sophie Greistorfer ◽  
Margret Eckhard ◽  
...  

Novel medical bioadhesives are proposed to fulfil numerous ideals as being biocompatible, non-toxic, include tissue healing and regeneration characteristics, have high mechanical properties onto different surfaces and other important key features. Mussel-inspired adhesives have provided the basis for many new applications under wet conditions. In contrast, the defence secretion system in amphibians may provide potential for novel fast-curing secretion able to adhere to surfaces under dry conditions. With the microanatomical and histochemical characterization of the endemic Japanese Oita salamander Hynobius dunni details on its anatomical organization, the nature of the chemical composition of both glue-producing glands and its divergence to the other well-characterized species Plethodon shermani are discussed. The study shows that the cutaneous glands of both glue-producing salamanders (H. dunni and P. shermani) exhibit certain morphological and histochemical similarities. Nevertheless, clear differences exist between the two species, especially with regard to the sugar composition of the mucous glands and the pH level of the granular glands. Moreover, the adhesive secretions of H. dunni show a clear reactivity to Arnow staining (indicating the presence of L-DOPA), which is lacking in P. shermani. This is the first indication of the presence of L-DOPA in the adhesive secretions of a terrestrial vertebrate, which has thus far only been found for marine invertebrates, such as mussels and polychaetes.

Author(s):  
D. F. Blake ◽  
L. F. Allard ◽  
D. R. Peacor

Echinodermata is a phylum of marine invertebrates which has been extant since Cambrian time (c.a. 500 m.y. before the present). Modern examples of echinoderms include sea urchins, sea stars, and sea lilies (crinoids). The endoskeletons of echinoderms are composed of plates or ossicles (Fig. 1) which are with few exceptions, porous, single crystals of high-magnesian calcite. Despite their single crystal nature, fracture surfaces do not exhibit the near-perfect {10.4} cleavage characteristic of inorganic calcite. This paradoxical mix of biogenic and inorganic features has prompted much recent work on echinoderm skeletal crystallography. Furthermore, fossil echinoderm hard parts comprise a volumetrically significant portion of some marine limestones sequences. The ultrastructural and microchemical characterization of modern skeletal material should lend insight into: 1). The nature of the biogenic processes involved, for example, the relationship of Mg heterogeneity to morphological and structural features in modern echinoderm material, and 2). The nature of the diagenetic changes undergone by their ancient, fossilized counterparts. In this study, high resolution TEM (HRTEM), high voltage TEM (HVTEM), and STEM microanalysis are used to characterize tha ultrastructural and microchemical composition of skeletal elements of the modern crinoid Neocrinus blakei.


2000 ◽  
Vol 6 (3) ◽  
pp. 197-205 ◽  
Author(s):  
T. Jimenez ◽  
M.A. Martinez-Anaya

Water soluble pentosans (WSP) from doughs and breads made with different enzyme preparations are characterized according to extraction yield, sugar composition, xylose/arabinose ratio and molecular weight (MW) distribution. Extraction yield was greater for dough than for bread samples, ranging from 0.94 to 1.64%, but bread extracts had a higher purity. Percent of pentoses in purified WSP was greater in pentosanase supplemented samples (28-55%) than in control and amylase containing samples (23-32%). Major sugars were xylose and arabinose, but glucose and mannose also appeared in the extracts. The xylose/arabinose (Xyl/Ara) ratio was 1.3-1.6 and underwent small changes during processing. Enzyme addition caused an increase in Xyl/Ara ratio, attributable to a debranching of arabinoxylans (AX) with higher degree of Ara substitution by arabinofuranosidase. Addition of pentosanases had a significant effect in increasing WSP with MW over 39 000, whereas those of low MW changed only slightly. MW distribution depended on enzyme source, and whereas some enzymes showed activity during fermentation others increased their activity during baking. No synergistic effects were observed in studied variables due to the combination of amylases with pentosanases. Protein in WSP extracts eluted together with ferulic acid suggesting they were linked, but not associated with a determined carbohydrate fraction.


Author(s):  
Samar Shah ◽  
Yaling Liu ◽  
Walter Hu

Biosensor detection process involves binding between biomolecules in a solution and a functionalized sensor surface. These sensors are time and cost efficient, sensitive, and enable new applications in medicine, drug design, and environmental monitoring. In literatures, various biosensor designs have been proposed, such as planar electrodes, nanowire, and nanospheres for different applications. However, to fully realize the potentials of these biosensors for biomarker/nanoparticle detection, several challenges must be addressed. In particular, ultra-sensitive biosensors are needed for detection of ultra-low concentration biomarkers such as cancer markers for early disease detection. The goal of this paper is to understand the diffusion process of biomarkers in a liquid solution and the binding with nanosensor surface through a stochastic particle model.


1970 ◽  
Vol 2 (4) ◽  
pp. 263-274 ◽  
Author(s):  
S. Bradbury ◽  
W. D. Billington ◽  
D. R. S. Kirby ◽  
E. A. Williams

2018 ◽  
Vol 22 (1) ◽  
pp. 1-15 ◽  
Author(s):  
A Lemos ◽  
F Adam ◽  
K Moura ◽  
L Moraes ◽  
O Silva

Author(s):  
André Luis Araujo Minari ◽  
Ronaldo V. Thomatieli-Santos

Macrophages are one of the top players when considering immune cells involved with tissue homeostasis. Recently, increasing evidence has demonstrated that these macrophages could also present two major subsets during tissue healing; proliferative macrophages (M1-like), which are responsible for increasing myogenic cell proliferation, and restorative macrophages (M2-like), which are accountable for the end of the mature muscle myogenesis. The participation and characterization of these macrophage subsets is critical during myogenesis, not only to understand the inflammatory role of macrophages during muscle recovery but also to create supportive strategies that can improve mass muscle maintenance. Indeed, most of our knowledge about macrophage subsets comes from skeletal muscle damage protocols, and we still do not know how these subsets can contribute to skeletal muscle adaptation. This narrative review aims to collect and discuss studies demonstrating the involvement of different macrophage subsets during the skeletal muscle damage/regeneration process, showcasing an essential role of these macrophage subsets during muscle adaptation induced by acute and chronic exercise programs.


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