Preliminary probiotic and technological characterization of Pediococcus pentosaceus strain KID7 and in vivo assessment of its cholesterol-lowering activity

Metallic nanoparticles, such as gold nanoparticles (AuNPs), have been extensively studied as drug delivery systems for various therapeutic applications. However, drug-loaded-AuNPs have been rarely explored in vivo for their effect on bacteria residing inside tissues. Ciprofloxacin (CIP) is a second-generation fluoroquinolone with a broad-spectrum of antibiotic properties devoid of developing bacteria resistance. This research is focused on the synthesis and physical characterization of Ciprofloxacin-loaded gold nanoparticles (CIP-AuNPs) and their effect on the colonization of Enterococcus faecalis in the liver and kidneys of mice. The successfully prepared CIP-AuNPs were stable and exerted enhanced in vitro antibacterial activity against E. faecalis compared with free CIP. The optimized CIP-AuNPs were administered (500 µg/Kg) once a day via tail vein to infected mice for eight days and were found to be effective in eradicating E. faecalis from the host tissues. Moreover, unlike CIP, CIP-AuNPs were non-hemolytic. In summary, this study demonstrated that CIP-AuNPs are promising and biocompatible alternative therapeutics for E.-faecalis-induced infections resistant to conventional drugs (e.g., beta-lactams and vancomycin) and should be further investigated.

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Characterization of Photo-Oxidized Small Bore Grafts

10.1115/imece1999-0396 ◽

1999 ◽

Author(s):

Diane L. Hern-Anderson ◽

Anne Marie J. Hernandez ◽

John P. Ranieri ◽

Steven Weinberg

Keyword(s):

Vascular Graft ◽

Covalent Modification ◽

Fresh Tissue ◽

Burst Strength ◽

Photo Oxidation ◽

Modification Process ◽

In Vivo Assessment

Abstract Many have hypothesized that both chemical and mechanical material properties are important in the design of a small bore vascular graft. In an attempt to address both issues, a xenogeneic graft crosslinked by photo-oxidation and covalently modified with heparin was developed. The compliance, burst strength, and suture pull strength of photo-oxidized (PhotoFix®) and heparin-modified photo-oxidized ovine carotid tissue were tested in vitro. The heparin modification process was found to have no statistically significant effect on any of these properties. The shrink temperature of fresh, photo-oxidized, and heparin-modified photo-oxidized tissue was assessed using DSC. A small increase in the shrink temperature of the heparin-modified photo-oxidized tissue over the fresh tissue was found to be statistically significant (P < 0.05). In vivo assessment of unmodified, heparin-soaked, and heparin-modified PhotoFix ovine grafts indicated that covalent modification of the grafts was imperative for the graft’s success.

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An in vivo assessment of the cholesterol-lowering efficacy of Lactobacillus plantarum ECGC 13110402 in normal to mildly hypercholesterolaemic adults

PLoS ONE ◽

10.1371/journal.pone.0187964 ◽

2017 ◽

Vol 12 (12) ◽

pp. e0187964 ◽

Cited By ~ 35

Author(s):

Adele Costabile ◽

Ivan Buttarazzi ◽

Sofia Kolida ◽

Sara Quercia ◽

Jessica Baldini ◽

...

Keyword(s):

Lactobacillus Plantarum ◽

Cholesterol Lowering ◽

In Vivo Assessment

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Screening for Cholesterol-Lowering Probiotics from Lactic Acid Bacteria Isolated from Corn Silage Based on Three Hypothesized Pathways

International Journal of Molecular Sciences ◽

10.3390/ijms20092073 ◽

2019 ◽

Vol 20 (9) ◽

pp. 2073 ◽

Cited By ~ 7

Author(s):

Changlu Ma ◽

Shuwen Zhang ◽

Jing Lu ◽

Cai Zhang ◽

Xiaoyang Pang ◽

...

Keyword(s):

Bile Salt ◽

Down Regulation ◽

Cholesterol Removal ◽

Cholesterol Lowering ◽

Probiotic Potential ◽

Cholesterol Levels ◽

Bile Salt Deconjugation ◽

Lowering Activity

A total of 85 strains of lactic acid bacteria were isolated from corn silage in this study and analyzed in vitro for their cholesterol removal, NPC1L1 protein down-regulation and bile salt deconjugation ability, respectively. Nineteen strains were selected for further analysis for their probiotic potential. Finally, 3 strains showing better probiotic potential were evaluated for their cholesterol-lowering activity in hamsters. The strains showing the greater cholesterol removal and NPC1L1 protein down-regulation activity had no significant effects on serum and hepatic cholesterol levels in hamsters (p > 0.05). However, Lactobacillus plantarum CAAS 18008 (1 × 109 CFU/d) showing the greater bile salt deconjugation ability significantly reduced serum low-density lipoprotein cholesterol, total cholesterol, and hepatic total cholesterol levels by 28.8%, 21.7%, and 30.9%, respectively (p < 0.05). The cholesterol-lowering mechanism was attributed to its bile salt hydrolase activity, which enhanced daily fecal bile acid excretion levels and thereby accelerated new bile acid synthesis from cholesterol in liver. This study demonstrated that the strains showing greater cholesterol removal and NPC1L1 protein down-regulation activity in vitro hardly reveal cholesterol-lowering activity in vivo, whereas the strains showing greater bile salt deconjugation ability in vitro has large potential to decrease serum cholesterol levels in vivo.

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