scholarly journals Bacterial Heavy-Metal and Antibiotic Resistance Genes in a Copper Tailing Dam Area in Northern China

2019 ◽  
Vol 10 ◽  
Author(s):  
Jianwen Chen ◽  
Junjian Li ◽  
Hong Zhang ◽  
Wei Shi ◽  
Yong Liu
Keyword(s):  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Northern China ◽  
Tailing Dam

scholarly journals Occurrence and distribution of antibiotic resistance genes in lakes and reservoirs from water-receiving area of Eastern Route of the South-to-North Water Diversion Project, Northern China

2020 ◽  
Vol 20 (8) ◽  
pp. 3029-3037
Author(s):  
Wei Hou ◽  
Fang Hu ◽  
Shaohua Sun ◽  
Xiaotong Dong ◽  
Mingquan Wang ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Local Level ◽  
Resistance Mechanism ◽  
Antibiotic Resistance Genes ◽  
Northern China ◽  
Water Diversion ◽  
Contamination Level ◽  
North Water ◽  
Study Sites

Abstract To characterize antibiotic resistance genes (ARGs) in surface waterbodies in Shandong Province, and to understand the occurrence, diversity and resistance mechanism of ARGs, nine aquatic ecosystems were selected and evaluated regarding occurrence of ARGs in the period from 2018 to 2019. Thirty-three types and 242 subtypes of ARGs were detected in the nine waterbodies with the most subtypes in Wohushan (242) and Jihongtan (235), and the fewest ARGs in Datun (213) and Mishan (221). Among the top nine ARG types, MacB, BcrA, CarA and TetW were the dominant ARGs in these samples. Four groups of the study sites were distinguished by cluster analysis, indicating different distribution characteristics of ARGs in these waterbodies, and regional differences in ARG contamination. Efflux pumps were the predominant resistance mechanism in all the waterbodies in terms of both subtype number (49.2%) and abundance (70.4%), followed by operon, antibiotic inactivation and others. Our findings provide comprehensive information to better understand the contamination level of ARGs on a local level as well as for global considerations, and may be practically helpful for the prevention and control of ARG pollution.

scholarly journals Tracking antibiotic resistance genes (ARGs) during earthworm conversion of cow dung in northern China

2021 ◽  
Vol 222 ◽  
pp. 112538
Author(s):  
Xueli Tian ◽  
Bingjun Han ◽  
Junfeng Liang ◽  
Fengxia Yang ◽  
Keqiang Zhang
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Northern China ◽  
Cow Dung

Metallic nanoparticles and ions accelerate the uptake of extracellular antibiotic resistance genes through transformation

2020 ◽  
Author(s):  
Jianhua Guo ◽  
Shuai Zhang ◽  
Ji Lu ◽  
Yue Wang ◽  
Willy Verstraete ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Cell Membrane ◽  
Resistance Genes ◽  
Natural Transformation ◽  
Antibiotic Resistance Genes ◽  
Acinetobacter Baylyi ◽  
Ag Nps ◽  
Zno Nps ◽  
Cuo Nps

Abstract Background: Antibiotic resistance genes (ARGs), heavy metal ions and nanoparticles (NPs) are emerging and ubiquitous contaminants in the environment. However, little is known about whether heavy metal-based NPs or ions could facilitate the dissemination of ARGs through natural transformation. This study evaluated the contributions of heavy metal-based NPs (Ag NPs, CuO NPs and ZnO NPs) and their ion forms (Ag + , Cu 2+ and Zn 2+ ) to the transformation of extracellular ARGs in Acinetobacter baylyi ADP1. Results: We found that these commonly-used NPs and ions from environmentally relevant concentrations can significantly promote the natural transformation frequency of ARGs by a factor of 11.0-folds, which is comparable to the effects of antibiotics. The enhanced transformation by Ag NPs, CuO NPs, Ag + and Cu 2+ was primarily associated with reactive oxygen species (ROS) over-production and cell membrane damage, which was also evident from up-regulations of both transcription and translation of ROS and outer membrane-related genes. Additionally, transmission electron microscope imaging revealed the roughened cell membrane after Ag NPs, CuO NPs, Ag + and Cu 2+ exposure. ZnO NPs and Zn 2+ might increase the natural transformation rate by stimulating the stress response and ATP synthesis. All tested NPs and ions resulted in up-regulating the competence and SOS response-associated genes. Conclusions: Our results demonstrate that Ag, CuO and ZnO-based NPs/ions from environmental concentrations could promote the natural transformation of plasmid-encoded ARGs into naturally competent A. baylyi . Our findings provide insights into the contributions of heavy metals and NPs to the spread of antibiotic resistance.

scholarly journals The Impact of Intensive Fish Farming on Pond Sediment Microbiome and Antibiotic Resistance Gene Composition

2021 ◽  
Vol 8 ◽  
Author(s):  
Eglė Lastauskienė ◽  
Vaidotas Valskys ◽  
Jonita Stankevičiūtė ◽  
Virginija Kalcienė ◽  
Vilmantas Gėgžna ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Heavy Metal Contamination ◽  
Production Systems ◽  
Antibiotic Resistance Gene ◽  
Antibiotic Resistance Genes ◽  
Fish Farming ◽  
Surrounding Water ◽  
The Impact

Aquaculture is a fast-growing animal food sector, and freshwater fish farming is particularly common in Central and Eastern Europe. As the biodiversity of fishery ponds is changed toward fulfilling the industrial needs, precautions should be taken to keep the system sustainable and protect the adjacent environment from possible damage. Due to risk of infectious diseases, antibiotics are used in aquaculture production systems. The constant exposure to antimicrobials can contribute to the rise of antibiotic resistance in aquaculture products and the adjacent ecosystems, with possibility of dissemination to the wider environment as well as between animals and humans. Even though previous studies have found antibiotic resistance genes in the sediments and water of farming ponds, the tendency and direction of spreading is not clear yet. The objective of this project was to evaluate the influence of intensive fish farming on the condition of water bodies used for the aquaculture and the environment, concentrating on the impact of the aquaculture on the surrounding water ecosystems as well as the possibility of transferring the pollutants and antibiotic resistance genes to both environment and the human hosts. Combined measurement of antibiotic and heavy metal contamination, toxicity assessment, microorganism diversity, and the detection of common antibiotic resistance genes was performed in the sediments of one fishery farm ponds as well as sampling points upstream and downstream. All the tested sediment samples did not show significantly elevated heavy metal concentrations and no substantial veterinary antibiotic pollution. From the antibiotic resistance genes tested, the presence of aminoglycoside and β-lactam resistance determinants as well as the presence of integrons could be of concern for the possibility of transfer to humans. However, despite the lack of heavy metal and antibiotic pollution, the sediments showed toxicity, the cause of which should be explored more.

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