scholarly journals Whole Genome Sequencing and Root Colonization Studies Reveal Novel Insights in the Biocontrol Potential and Growth Promotion by Bacillus subtilis MBI 600 on Cucumber

2021 ◽  
Vol 11 ◽  
Author(s):  
Anastasios Samaras ◽  
Marios Nikolaidis ◽  
Maria Luisa Antequera-Gómez ◽  
Jesus Cámara-Almirón ◽  
Diego Romero ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Root Colonization ◽  
Growth Promotion ◽  
Laser Scanning Microscopy ◽  
Main Role ◽  
Agricultural Practice ◽  
Sequencing Analysis ◽  
Colonization Ability ◽  
Cucumber Roots

Bacillus spp. MBI 600 is a gram-positive bacterium and is characterized as a PGPR strain involved in plant growth promotion and control of various plant pathogens which has recently been introduced into the agricultural practice. In this study we performed a Next Generation Sequencing analysis, to analyze the full genome of this microorganism and to characterize it taxonomically. Results showed that MBI 600 strain was phylogenetically close to other Bacillus spp. strains used as biocontrol agents and identified as B. subtilis. GOG analysis showed clusters contributed to secondary metabolites production such as fengycin and surfactin. In addition, various genes which annotated according to other plant-associated strains, showed that play a main role in nutrient availability from soil. The root colonization ability of MBI 600 strain was analyzed in vivo with a yellow fluorescence protein (yfp) tag. Confocal laser scanning microscopy of cucumber roots treated with yfp-tagged MBI 600 cells, revealed that the strain exhibits a strong colonization ability of cucumber roots, although it is affected significantly by the growth substrate of the roots. In vitro and in planta experiments with MBI 600 strain and F. oxysporum f.sp. radicis cucumerinum and P. aphanidernatum, showed a high control ability against these soilborne pathogens. Overall, our study demonstrates the effectiveness of MBI 600 in plant growth promotion and antagonism against different pathogens, highlighting the use of this microorganism as a biocontrol agent.

scholarly journals Whole Genome Sequencing and Genome Annotation of PGPR ‘Exiguobacterium sp. TNDT2’ Isolated from Dates Palm Tree Rhizospheric Soil

2021 ◽  
Author(s):  
Thennarasu Sugumar ◽  
Punithavathi Srinivasan ◽  
B. Muthukumar ◽  
E. Natarajan
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Genome Annotation ◽  
Growth Promotion ◽  
Multi Drug Resistance ◽  
Palm Tree ◽  
Whole Genome ◽  
Agricultural Practice ◽  
Plant Growth Promoting ◽  
Growth Promoting

Background: Dates palm is one of the most economically important plant mainly cultivated in Northern Africa, Middle East and South Asia. India is the largest importer of date fruit. In India, Dates palm are cultivated majorly in Gujarat and Rajasthan. Dates farmers facing several problem in India due to lack of scientific resources. Plant growth promoting rhizobacteria (PGPR) are naturally associated with plants and it improves plant growth and yield by providing growth supplements, increasing tolerance to stressful conditions and providing resistance to fungal/bacterial diseases. We have isolated a PGPR belonging to Exiguobacterium species TNDT2 from Indian dates palm Phoenix dactylifera, in Dindigul region, Tamilnadu, India. The organism’s genome was sequenced and identified several potential plant growth promoting (PGP) genes. Methods: The organisms genome was sequenced using Whole genome shotgun sequencing method in Illumina platform. Sequences are analysed using various bioinformatics tools and assembled using Velvet assembler. Contigs are annotated using RAST server and deposited in NCBI. Result: The isolated strain revealed various genetic determinants required for plant growth promotion. This study presents the first report of Exiguobacterium TNDT2 genome from Dates tree rhizosphere. Whole genome analysis and genome annotation reveals that, its genome consist of a 2,891,840 bp chromosome encoding over 3062 proteins, with a 51.63% GC content. Strain TNDT2 encodes a wide repertoire of proteins for plant growth promotion, heavy metal detoxification (cadmium, arsenic, mercury, copper and tellurite), Multi-drug resistance and stress resistance (Heat, cold and salt). Based on this study, Exiguobacterium sp. TNDT2 can be recognized as an important organism with a potential to be incorporated into agricultural practice of Date palm.

scholarly journals Characterization of streptomycetes with potential to promote plant growth and biocontrol

2008 ◽  
Vol 65 (1) ◽  
pp. 50-55 ◽  
Author(s):  
Carla da Silva Sousa ◽  
Ana Cristina Fermino Soares ◽  
Marlon da Silva Garrido
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Indole Acetic Acid ◽  
Extracellular Enzymes ◽  
Root Colonization ◽  
Phosphate Solubilization ◽  
Culture Media ◽  
Growth Promotion ◽  
Salinity Levels

Studies with streptomycetes in biocontrol programs and plant growth promotion are presented as technological alternatives for environmental sustainable production. This work has the objective of characterizing six isolates of streptomycetes aiming the production of extracellular enzymes, indole acetic acid, capacity for phosphate solubilization, root colonization and growth under different pH and salinity levels. For detection of enzyme activity the isolates were grown in culture media with the enzyme substrates as sole carbon source. The root colonization assay was performed on tomato seedlings grown on 0.6% water-agar medium. Growth under different pH and salinity levels was evaluated in AGS medium with 1%, 1.5%, 2%, 2.5%, and 3% NaCl, and pH levels adjusted to 5.0, 5.5, 6.0, 6.5, and 7.0. All isolates produced the enzymes amylase, catalase, and lipase, as well as indole acetic acid. With one exception (AC-92), all isolates presented cellulolytic and chitinolytic activity, and only AC-26 did not show xylanolytic activity. The isolates AC-147, AC-95, and AC-29 were the highest producers of siderophores. The isolates AC-26 and AC-29 did not show capacity for phosphate solubilization. All isolates colonized tomato roots in vitro, and AC-92 grew under all pH and salinity levels tested. The streptomycetes tested were considered as potential biocontrol and plant growth promotion agents.

scholarly journals Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape (Brassica napus L.) against Fungal Pathogens

2020 ◽  
Vol 21 (22) ◽  
pp. 8740
Author(s):  
Daria Chlebek ◽  
Artur Pinski ◽  
Joanna Żur ◽  
Justyna Michalska ◽  
Katarzyna Hupert-Kocurek
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Oilseed Rape ◽  
Plant Growth Promotion ◽  
Fungal Pathogens ◽  
Growth Promotion ◽  
Genome Mining ◽  
Plant Colonization ◽  
Brassica Napus L

Endophytic bacteria hold tremendous potential for use as biocontrol agents. Our study aimed to investigate the biocontrol activity of Pseudomonas fluorescens BRZ63, a new endophyte of oilseed rape (Brassica napus L.) against Rhizoctonia solani W70, Colletotrichum dematium K, Sclerotinia sclerotiorum K2291, and Fusarium avenaceum. In addition, features crucial for biocontrol, plant growth promotion, and colonization were assessed and linked with the genome sequences. The in vitro tests showed that BRZ63 significantly inhibited the mycelium growth of all tested pathogens and stimulated germination and growth of oilseed rape seedlings treated with fungal pathogens. The BRZ63 strain can benefit plants by producing biosurfactants, siderophores, indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia as well as phosphate solubilization. The abilities of exopolysaccharide production, autoaggregation, and biofilm formation additionally underline its potential to plant colonization and hence biocontrol. The effective colonization properties of the BRZ63 strain were confirmed by microscopy observations of EGFP-expressing cells colonizing the root surface and epidermal cells of Arabidopsis thaliana Col-0. Genome mining identified many genes related to the biocontrol process, such as transporters, siderophores, and other secondary metabolites. All analyses revealed that the BRZ63 strain is an excellent endophytic candidate for biocontrol of various plant pathogens and plant growth promotion.

scholarly journals Streptomyces consortia-mediated plant defense against Fusarium wilt and plant growth-promotion in chickpea

2021 ◽  
pp. 104961
Author(s):  
Sravani Ankati ◽  
Vadlamudi Srinivas ◽  
Sambangi Pratyusha ◽  
Subramaniam Gopalakrishnan
Keyword(s):  
Plant Growth ◽  
Plant Defense ◽  
Fusarium Wilt ◽  
Plant Growth Promotion ◽  
Growth Promotion
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