The Origin and Molecular Epidemiology of Dengue Fever in Hainan Province, China, 2019

There was an outbreak of Dengue fever on September 5, 2019, in Hainan Province, which has not been endemic for 28 years. We aim to describe the clinical and epidemiological features of the 2019 outbreak in Hainan Province and identify the cause. All type 1 Dengue fever cases that occurred in this outbreak of Hainan exhibited mild clinical symptoms. The epidemiological investigations indicate that the outbreak might originate from workers in the Xiuying area, Haikou City, form a concentrated outbreak, and then spread out. Bayesian phylogenies results and epidemiological data were used to infer a likely series of events for the dengue virus’s potential spread and trace the possible sources. The strains’ sequences were close to a sequence from the nearby Guangdong province, supporting the hypothesis that the dengue virus was imported from Guangdong province and then spread across Hainan province. Furthermore, it is interesting that two other strains didn’t group with this cluster, suggesting that additional introduction pathways might exist. The study indicated that the dengue fever epidemic presented two important modes in Hainan. Firstly, epidemics prevalence was caused by imported cases, and then endogenous epidemics broke out in the natural epidemic focus.

Download Full-text

New dengue virus Type-1 strain; recombinant DEN1 protein expression; potential in recombinant vaccine construction, virus detection and dengue fever diagnosis and therapy

Vaccine ◽

10.1016/0264-410x(94)90277-1 ◽

1994 ◽

Vol 12 (7) ◽

pp. 671

Keyword(s):

Dengue Virus ◽

Protein Expression ◽

Dengue Fever ◽

Virus Type ◽

Virus Detection ◽

Recombinant Vaccine ◽

Diagnosis And Therapy ◽

Expression Potential

Download Full-text

Molecular characterization of the E gene of dengue virus type 1 isolated in Guangdong province, China, in 2006

Epidemiology and Infection ◽

10.1017/s0950268808000617 ◽

2008 ◽

Vol 137 (1) ◽

pp. 73-78 ◽

Cited By ~ 23

Author(s):

K. ZHENG ◽

H.-Q. ZHOU ◽

J. YAN ◽

C.-W. KE ◽

A. MAEDA ◽

...

Keyword(s):

Dengue Virus ◽

General Circulation ◽

Genetic Relationships ◽

Guangdong Province ◽

Envelope Gene ◽

Serum Samples ◽

Guangzhou City ◽

Imported Case

SUMMARYWe determined the genetic relationships and origin of the dengue virus (DENV) responsible for an outbreak of dengue fever (DF) in Guangdong province, China, in 2006. Five DENV type 1 (DENV-1) isolates were obtained from human serum samples collected from DF patients during the outbreak. The nucleotide sequences of the E (envelope) gene were compared with those of 48 previous DENV-1 isolates: 18 from Guangdong province, one from Fujian province, one from Zhejiang province, and 28 from other countries in the South Asian region. The results suggested that four DENV-1 isolates identified in Guangdong province in 2006 might be in general circulation there, although these DENV-1 viruses may have been originally introduced into the province from other countries. In contrast, one isolate from Guangzhou city in 2006, may have been introduced by a recently imported case from Cambodia.

Download Full-text

Homology of complete genome sequences for dengue virus type-1, from dengue-fever- and dengue-haemorrhagic-fever-associated epidemics in Hawaii and French Polynesia

Annals of Tropical Medicine and Parasitology ◽

10.1179/136485910x12647085215570 ◽

2010 ◽

Vol 104 (3) ◽

pp. 225-235 ◽

Cited By ~ 12

Author(s):

A. Imrie ◽

C. Roche ◽

Z. Zhao ◽

S. Bennett ◽

M. Laille ◽

...

Keyword(s):

Dengue Virus ◽

Dengue Fever ◽

Virus Type ◽

Complete Genome ◽

French Polynesia ◽

Dengue Haemorrhagic Fever ◽

Haemorrhagic Fever ◽

Genome Sequences

Download Full-text

Fatal and mild primary dengue virus infections imported to Norway from Africa and south-east Asia, 2008-2010

Eurosurveillance ◽

10.2807/ese.15.38.19666-en ◽

2010 ◽

Vol 15 (38) ◽

Cited By ~ 8

Author(s):

K Vainio ◽

S Noraas ◽

M Holmberg ◽

H Fremstad ◽

M Wahlstrøm ◽

...

Keyword(s):

Dengue Virus ◽

Dengue Fever ◽

Fatal Case ◽

Fatal Outcome ◽

Dengue Shock Syndrome ◽

Virus Infections ◽

Virus Serotype ◽

Serotype 1 ◽

Type 3

Between 2008 and 2010, eight cases of viraemic dengue fever in travellers were diagnosed in Norway. They had returned from Eritrea, Thailand and Indonesia. All cases were primary dengue infections, seven non-complicated dengue fever and one dengue shock syndrome with a fatal outcome. Four patients were infected with dengue virus serotype 1, one with type 2 and three with type 3. Two cases from Thailand, the fatal case and the two imported from Eritrea were infected with type 1.

Download Full-text

Clinical profile of dengue fever infection in King Abdul Aziz University Hospital Saudi Arabia

The Journal of Infection in Developing Countries ◽

10.3855/jidc.1038 ◽

2010 ◽

Vol 4 (08) ◽

pp. 503-510 ◽

Cited By ~ 15

Author(s):

Maimoona M Ahmed

Keyword(s):

Dengue Virus ◽

Dengue Fever ◽

Clinical Symptoms ◽

Degradation Products ◽

Disease Incidence ◽

Inverse Correlation ◽

Immunoglobulin M ◽

Clinical Profile ◽

Direct Bilirubin ◽

University Hospital

Background: The study aimed to compare the clinical profile of all patients diagnosed with dengue viral infection at King Abdul Aziz University Hospital (KAAUH), during 2005-2008. Methodology: This retrospective study included 147 patients infected with dengue virus, age ≤ 16 years. Laboratory and haematological data were included. Results: Two peaks of infection occurred during 2006 and another two in 2008. Common clinical symptoms were fever, vomiting, and abdominal pain. Common haematological abnormalities were thrombocytopenia and leucopoenia. Differences existed between the years in the percentage of patients with fever, elevated alanine aminotransferase (ALT), direct bilirubin, lactate dehydrogenase (LDH), fibrin degradation products (FDPs), and haemoglobin (Hb) levels. Differences were found in nationalities between the years, but patient nationality had no effect on disease incidence. Differences were noted in the percentages of patients' immunoglobulin M (IgM) and polymerase chain reaction (PCR) positive. There was a slight inverse correlation of IgM positive with patient age. PCR, fever, ALT, direct bilirubin, LDH, FDPs, Hb, blood transfusion, and platelet transfusion showed no correlation with age or nationality. In 2005, all the patients survived, but there were 4.55%, 25%, and 2.7% deaths during 2006-2008. Conclusions: Significant differences in the clinical presentation of dengue virus (DENV) infection, indicative of a variation in disease severity from dengue fever (DF) to dengue hemhorrhagic fever (DHF)/dengue shock syndrome (DSS), were noted over the years. Possible reasons are infection with different serotypes, concurrent/sequential infection of more than one serotype, and differences in host immune responses associated with host genetic variations.

Download Full-text

Dengue fever in Morocco: result of surveillance during the year 2017 and first imported cases

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20180633 ◽

2018 ◽

Vol 6 (3) ◽

pp. 1029 ◽

Cited By ~ 1

Author(s):

Tahar Bajjou ◽

Youssef Akhouad ◽

Farida Hilali ◽

Safae Elkochri ◽

Abdelilah Laraqui ◽

...

Keyword(s):

Dengue Virus ◽

Dengue Fever ◽

Cote D'ivoire ◽

Côte D’Ivoire ◽

Clinical Signs ◽

Denv Infection ◽

Cote D’Ivoire ◽

Côte D'ivoire ◽

Imported Cases ◽

The Town

Dengue is a significant mosquito-borne infection in humans, and its worldwide prevalence is rapidly increasing. The vector aedes albopictus has been revealed recently in the town of Rabat. Morocco established a program of active surveillance of dengue fever comprising many hospitals and laboratories across the kingdom. The purpose of this work is to describe the result of the surveillance of the dengue virus (DENV) infection during the year of 2017 among Moroccans and tourists who presented in our hospital with clinical signs of infection and to report the first confirmed positive cases of Dengue.From 20 December 2016 to 20 December 2017, 21 patients were hospitalized for suspicion of DENV infection. Half of them were returning from Côte d’Ivoire which is a popular tourist and business country for Moroccans and where an outbreak of DENV was confirmed on July 2017. Fever, headache, arthralgia-myalgia and malaise in addition to the notion of return from an endemic country justify in clinicians the demand for analysis of detection of dengue virus by RT-PCR.Dengue infection was confirmed in two patient both coming from Côte d’Ivoire, a Moroccan and an Ivorian who were staying in Abidjan during the period of the outbreak of 2017.

Download Full-text

Epidemiological analysis of the Kaohsiung city strategy for dengue fever quarantine and epidemic prevention

10.21203/rs.2.15494/v2 ◽

2019 ◽

Author(s):

Chun-Hong CHEN ◽

Chao Ying Pan ◽

Wei-Liang Liu ◽

Matthew P. Su ◽

Te Pin Chang ◽

...

Keyword(s):

Disease Control ◽

Dengue Fever ◽

Public Health Problem ◽

Epidemiological Data ◽

Health Department ◽

Local Communities ◽

Surveillance Systems ◽

Important Public Health Problem ◽

Imported Cases ◽

Kaohsiung City

Abstract Background. Dengue is endemic in over 100 countries and is an important public health problem worldwide. Dengue is not considered endemic in Taiwan however, with importation of dengue viruses from neighboring countries via close commercial links and air travel believed to be the cause of local outbreaks. Disease control efforts have therefore focused on preventing the importation of dengue into Taiwan. Here, we investigate the relationships between numbers of imported and indigenous dengue cases to test the validity of this strategy. Methods. Dengue-fever case data from between 2013 and 2018 was obtained from the surveillance systems of the Taiwan Center for Disease Control and Kaohsiung City Health department. Standard epidemiological data, including the monthly numbers of indigenous and imported dengue cases, was calculated. Potential associations between the number of indigenous and imported cases were investigated using correlation analyses. Results. We identified a possible relationship between the period of disease concealment and the number of reported imported dengue cases which resulted in indigenous dengue-fever epidemics within local communities. Further analysis of confirmed dengue cases during previous dengue-fever epidemics in Kaohsiung City found that the risk of indigenous dengue-fever may be related to the likelihood of patients suffering from imported cases of dengue-fever staying within local communities. Conclusion. Given the correlations found between imported and indigenous dengue-fever cases, as well as the relationship between disease concealment period and risk of indigenous dengue-fever, the major priorities for disease control remain prevention of disease importation and efficient identification of dengue cases within high-risk communities.

Download Full-text

VERIFICATION OF DENGUE FEVER CASES IMPORTED TO THE TERRITORY OF THE SOUTH FAR EAST

Russian Clinical Laboratory Diagnostics ◽

10.18821/0869-2084-2020-65-6-382-386 ◽

2020 ◽

Vol 65 (6) ◽

pp. 382-386

Author(s):

G. N. Leonova

Keyword(s):

Dengue Virus ◽

Dengue Fever ◽

Far East ◽

Significant Proportion ◽

Rapid Test ◽

The South ◽

Blood Samples ◽

Genotype 2 ◽

Imported Cases ◽

Ns1 Antigen

There are various diagnostic and research methods for detecting cases of Dengue fever, the effectiveness of which is given in this work. Materials and methods. On biomaterial from 70 people, verification of imported cases of Dengue fever into the south of the Far East from 2012 to 2019 is shown. Serological and virological methods were used, as well as PCR. Results. Using the immunochromatographic rapid test, the Dengue virus (DENV) NS1 antigen and antibodies to DENV (IgM and IgG) were detected in human blood. We examined 12 patients from the infectious diseases department with unknown fever and the blood of 58 people who applied to clinics in Vladivostok after returning from tourist trips. Dengue fever was diagnosed in 23 patients (32.8%), of which antigen was detected in 56%, IgM antibodies in 91.3% and IgG in 52.1%. In 2 cases (8.7%), only antigen was detected in patients. Three strains of the pathogen were isolated by virological methods from 18 blood samples, two of which turned out to be the DENV of the 1st genotype and one - of the DENV of the 2nd genotype. Using RT-PCR, 38 blood samples were tested positive in the immunochromatographic rapid test, of which in 16 cases (42.1%) a DENV marker was detected, in 11 cases it was genotype 1, in three cases genotype 2, and one each - genotypes 3 and 4.Conclusions: 1. The most reliable method of rapid verification (in 100%) the primary infection DENV was the comprehensive determination of antigen and antibodies of the IgM class; 2. With antigenemia, blood should be used to isolate the virus, as well as to diagnose the disease by PCR and to establish the genotype of the DENV; 3. When using only PCR to indicate Dengue virus, a significant proportion of the disease cases will not be diagnosed.

Download Full-text

Clinical profile of dengue fever infection in patients admitted in NC Medical College, Haryana in the year 2019

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20203458 ◽

2020 ◽

Vol 8 (8) ◽

pp. 3027

Author(s):

Mohd Y. Shah ◽

Faisal Y. Shah ◽

Ifrah S. Kitab ◽

Faizan Y. Shah

Keyword(s):

Dengue Virus ◽

Dengue Fever ◽

Dengue Hemorrhagic Fever ◽

Clinical Symptoms ◽

Wide Spectrum ◽

Dengue Shock Syndrome ◽

Hemorrhagic Fever ◽

Clinical Profile ◽

Host Immune Responses ◽

Medical College

Background: Dengue infections can result in a wide spectrum of disease severity ranging from an influenza-like illness (dengue fever; DF) to the life-threatening dengue hemorrhagic fever (DHF)/dengue shock syndrome (DSS). The study was aimed to compare the clinical profile of all patients diagnosed with dengue viral infection at NCMC.Methods: This retrospective study included 24 patients infected with dengue virus, aged 19 years to 45 years. Laboratory and haematological data were included.Results Peak of infection occurred in November 2019 and no cases were recorded in October 2019. Common clinical symptoms were fever, joint pains, headache and rash. Common haematological abnormalities were thrombocytopenia. All patients survived. There was no case of dengue hemorrhagic fever or dengue shock syndrome.Conclusions: Significant differences in the clinical profile is possibly because of infection with different serotypes of dengue virus (DENV), concurrent/sequential infection of more than one serotype, and differences in host immune responses associated with host genetic variations.

Download Full-text