scholarly journals The Effects of Delivery Mode on the Gut Microbiota and Health: State of Art

2021 ◽  
Vol 12 ◽  
Author(s):  
Chenchen Zhang ◽  
Lixiang Li ◽  
Biying Jin ◽  
Xinyan Xu ◽  
Xiuli Zuo ◽  
...  
Keyword(s):  
Cesarean Section ◽  
Gut Microbiota ◽  
Metabolic Disorders ◽  
Neonatal Period ◽  
Intestinal Epithelial Cells ◽  
Delivery Mode ◽  
Health State ◽  
Intestinal Epithelial ◽  
Prevention And Treatment ◽  
State Of Art

The delivery mode is an important factor driving alteration in the gut microbiota during the neonatal period. Several studies prove that the alteration of gut microbiota induced by cesarean section could influence the activation of intestinal epithelial cells and the development of immune system. Further, some autoimmune and metabolic disorders may be related to the microbiota dysbiosis in infants caused by cesarean section. It is noteworthy that probiotics could promote the intestinal microecology, which may further prevent and treat cesarean section related diseases. This review summarized the great significance of delivery mode on microbiota and health, as well as provided clinically feasible methods for the prevention and treatment of cesarean section related gut diseases.

scholarly journals 376 – Interleukin-33 Promotes Reg3G Expression in Intestinal Epithelial Cells and Regulates Gut Microbiota

2019 ◽  
Vol 156 (6) ◽  
pp. S-78
Author(s):  
Wenjing Yang ◽  
Yi Xiao ◽  
Xiangsheng Huang ◽  
Suxia Yao ◽  
Zhanju Liu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Gut Microbiota ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Interleukin 33

scholarly journals Gut Microbiota Shift in Obese Adolescents Born by Cesarean Section

2020 ◽  
Vol 10 (4) ◽  
pp. 424-429
Author(s):  
Evgenia Novikova ◽  
Natalia Belkova ◽  
Anna Pogodina ◽  
Anastasia Romanitsa ◽  
Elizaveta Klimenko ◽  
...  
Keyword(s):  
Cesarean Section ◽  
Gut Microbiota ◽  
Postnatal Period ◽  
Human Reproduction ◽  
Rrna Gene ◽  
Delivery Mode ◽  
Illumina Platform ◽  
Scientific Center ◽  
Variable Regions ◽  
Obese Adolescents

Background: It is known that in the early postnatal period a variety of factors affect the gut microbiota (GM) composition, including delivery mode. The effect of delivery mode on the human GM in the late postnatal period remains unexplored. A shift of GM composition due to delivery mode may contribute to the development of obesity in adulthood. Methods and Results: The study included six adolescents aged between 11 and 17 years treated and examined at the Clinic of the Scientific Center for Family Health and Human Reproduction (Irkutsk, Russia) in 2016. Stool samples were collected following the standard operating procedures according to the International Human Microbiome Standards. Metasequencing of V3-V4 variable regions of the 16S rRNA gene was performed by the Novogene Company (China) on the Illumina platform. Bioinformatic analysis was done by the bri-shur.com services. Sequencing reads were presented as normalized values. In general, the GM composition of obese adolescents born by cesarean section was characterized by composition heterogeneity within the Bacteroidetes phylum and the dominance of certain phylotypes as signs of dysbiosis for each adolescent. We detected an increased abundance of phyla Bacteroides and Proteobacteria, and an absence of Tenericutes in obese adolescents born by Caesarean section. On the level of genera, the prevalence of Bacteroides and Bacteroides S24-7 phylotypes, and the absence of the RF39 phylotype, led to the GM shift associated with a cesarean section or obesity. Conclusion: Obese adolescents born by cesarean section delivery present the shift in GM composition.

scholarly journals Identification and characteristics of postnatal development-related long non-coding RNAs under microbiota dependent condition

2020 ◽  
Author(s):  
Miaomiao Zheng ◽  
Binbin Zhang ◽  
Yidan Zhang ◽  
Tingting Sun ◽  
Baozhong Hu
Keyword(s):  
Epithelial Cells ◽  
Gut Microbiota ◽  
Postnatal Development ◽  
Intestinal Epithelial Cells ◽  
Development Stage ◽  
Intestinal Epithelial ◽  
Early Postnatal Development ◽  
Development Stages ◽  
Large Numbers ◽  
Non Coding Rnas

Abstract BackgroundThe interplay of long-non coding RNAs (lncRNAs) and the intestinal microbiota may serve as an essential role in intestinal development and homeostasis. Microbiota could regulate a large numbers of lncRNAs expression in intestinal epithelial cells. However, the associations between lncRNAs and microbiota during early postnatal development stages are still need to understand. MethodsIn present study, the microbial effects on lncRNA of intestinal epithelial cells (IECs) during postnatal development stage were investigated. ResultsWe identified gut microbiota-specific lncRNAs in diverse postnatal development stages including week 1, week 4 and week 12/16 of mice. A large proportion of gut microbiota-specific lncRNAs only were differential expressed in a single postnatal development stage. Up- and down-regulated gut microbiota-specific lncRNAs both showed consistent expression pattern. We also constructed gut microbiota-specific lncRNAs and coding genes interacted co-expressed networks. Functional analysis indicated that gut microbiota-specific lncRNAs were associated with ABC transporters. ConclusionsIn summary, the present study characterizes the landscape of lncRNAs associated with gut microbiota in different postnatal development stages. It provide assistance for exploring the relationships among lncRNAs, gut microbiota and postnatal development stages.

Effect of Delivery Mode and Nutrition on Gut Microbiota in Neonates

2019 ◽  
Vol 74 (2) ◽  
pp. 132-139 ◽  
Author(s):  
Shohei Akagawa ◽  
Shoji Tsuji ◽  
Chikushi Onuma ◽  
Yuko Akagawa ◽  
Tadashi Yamaguchi ◽  
...  
Keyword(s):  
Cesarean Section ◽  
Gut Microbiota ◽  
Bacterial Diversity ◽  
Mode Of Delivery ◽  
Influential Factors ◽  
Delivery Mode ◽  
Cesarean Section Delivery ◽  
Feeding Type ◽  
Stool Samples ◽  
Gut Microbiota Composition

Background/Aims: The mode of delivery (vaginal or cesarean section) and feeding type (breastfeeding or formula feeding) of neonates are considered the most influential factors in the development of gut microbiota. Objectives: This study investigated the effect of prebiotic-rich breast milk on overcoming gut microbiota dysbiosis. Method: Stool samples from 36 healthy Japanese neonates were obtained at 4 days and 1 month of age, and divided into 4 groups based on mode of delivery and feeding type. The gut microbiota composition and bacterial diversity were assessed using 16S rRNA sequencing. Results: At 4 days old, vaginally delivered neonates had a significantly higher diversity of bacteria than those born by cesarean section. Bacteroidales and Enterobacteriales were overrepresented in vaginally delivered neonates (p = 0.0031 and p = 0.011), while Bacillales and Lactobacillales were overrepresented in caesarean section delivered neonates (p = 0.012 and p = 0.0016). However, there was little difference in bacterial diversity and bacterial relative abundance at 1 month of age between groups. Conclusions: Cesarean section delivery appeared to reduce the diversity of neonate gut microbiota, resulting in dysbiosis, but this improved to the equivalent level seen in vaginally delivered infants by 1 month of age. Breastfeeding, even for short periods, may therefore improve neonate gut dysbiosis.

Faecal microRNAs: indicators of imbalance at the host-microbe interface?

2018 ◽  
Vol 9 (2) ◽  
pp. 175-183 ◽  
Author(s):  
G.M. Moloney ◽  
M.F. Viola ◽  
A.E. Hoban ◽  
T.G. Dinan ◽  
J.F. Cryan
Keyword(s):  
Epithelial Cells ◽  
Gut Microbiota ◽  
Antibiotic Treatment ◽  
Mirna Expression ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Sprague Dawley ◽  
Faecal Pellets ◽  
Non Invasive ◽  
Bodily Fluids

The enteric microbiota is characterised by a balance and composition that is unique to the host. It is important to understand the mechanisms through which the host can maintain the composition of the gut microbiota. MicroRNAs (miRNA) are implicated in intercellular communication and have been isolated from bodily fluids including stool. Recent findings suggest that miRNA produced by the host’s intestinal epithelial cells (IECs) participate in shaping the microbiota. To investigate whether miRNA expression was influenced by the gut microbiota we measured the expression of miRNAs expressed by intestinal epithelial cells in faeces. Specifically, we measured miRNA expression in faeces from germ-free (GF) and conventional mice and similarly in a rat model of antibiotic-mediated depletion of the gut microbiota control rats. In adult male GF and conventional mice and adult Sprague Dawley (SD) rats were treated with a combination of antibiotics for 8 weeks; total RNA was extracted from faecal pellets taken at week 0, 2, 4, 6 week 8 and the expression of let-7b-3p, miR-141-3p, miR-200a-3p and miR-1224-5p (miRNAs known to be expressed in IECs) were measured relative to U6 at each time point using qRT-PCR. In GF animals the expression of let-7b, miR-141 and miR-200a in faeces was lower compared to conventional mice. Following antibiotic-mediated depletion of gut microbiota, rats showed two divergent profiles of miRNA expression. Following two weeks of antibiotic treatment, the expression of let-7b and miR-1224 dropped significantly and remained low for the remainder of the study. The expression of miR-200a and miR-141 was significantly higher at week 2 than before antibiotic treatment commenced. Subsequently, the expression of miR-200a and miR-141 decreased at week 4 and continued to decrease at week 6. This data demonstrates that miRNAs can be used as an independent, non-invasive marker of microbial fluctuations along with gut pathology in the intestine.

scholarly journals Impact of delivery mode on oral health

2021 ◽  
Vol 67 (3) ◽  
pp. 139-142
Author(s):  
Ioanina Părlătescu ◽  
◽  
Dragoș Epistatu ◽  
Raluca Ema Pîrvu ◽  
Roxana Elena Bohîlțea ◽  
...  
Keyword(s):  
Immune System ◽  
Oral Health ◽  
Dental Caries ◽  
Oral Cavity ◽  
Cesarean Section ◽  
Gut Microbiota ◽  
Delivery Mode ◽  
The Moment ◽  
Route Of Delivery

The route of delivery influences the gut microbiota and the development of the immune system. At the moment of birth, cesarean section infants have a lower diversity of bacteria when compared to naturally born infants. The delivery mode does influence the oral cavity colonization of infants, but there is no relevant association with dental caries.

scholarly journals Gut Microbiota Dysbiosis in Systemic Lupus Erythematosus: Novel Insights into Mechanisms and Promising Therapeutic Strategies

2021 ◽  
Vol 12 ◽  
Author(s):  
Quanren Pan ◽  
Fengbiao Guo ◽  
Yanyan Huang ◽  
Aifen Li ◽  
Shuxian Chen ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Epithelial Cells ◽  
Gut Microbiota ◽  
Lupus Erythematosus ◽  
Intestinal Epithelial Cells ◽  
Therapeutic Strategies ◽  
Oral Antibiotic ◽  
Intestinal Epithelial ◽  
Systemic Lupus ◽  
Gut Microbiota Dysbiosis

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that was traditionally thought to be closely related to genetic and environmental risk factors. Although treatment options for SLE with hormones, immunosuppressants, and biologic drugs are now available, the rates of clinical response and functional remission of these drugs are still not satisfactory. Currently, emerging evidence suggests that gut microbiota dysbiosis may play crucial roles in the occurrence and development of SLE, and manipulation of targeting the gut microbiota holds great promises for the successful treatment of SLE. The possible mechanisms of gut microbiota dysbiosis in SLE have not yet been well identified to date, although they may include molecular mimicry, impaired intestinal barrier function and leaky gut, bacterial biofilms, intestinal specific pathogen infection, gender bias, intestinal epithelial cells autophagy, and extracellular vesicles and microRNAs. Potential therapies for modulating gut microbiota in SLE include oral antibiotic therapy, fecal microbiota transplantation, glucocorticoid therapy, regulation of intestinal epithelial cells autophagy, extracellular vesicle-derived miRNA therapy, mesenchymal stem cell therapy, and vaccination. This review summarizes novel insights into the mechanisms of microbiota dysbiosis in SLE and promising therapeutic strategies, which may help improve our understanding of the pathogenesis of SLE and provide novel therapies for SLE.

scholarly journals Exposure to the gut microbiota drives distinct methylome and transcriptome changes in intestinal epithelial cells during postnatal development

2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Wei-Hung Pan ◽  
Felix Sommer ◽  
Maren Falk-Paulsen ◽  
Thomas Ulas ◽  
Philipp Best ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Gut Microbiota ◽  
Postnatal Development ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial

scholarly journals Delivery Mode Affects Intestinal Microbial Composition and the Development of Intestinal Epithelial Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Linzheng Lyu ◽  
Xiaohong Zhou ◽  
Meng Zhang ◽  
Li Liu ◽  
Haiyue Niu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Vaginal Delivery ◽  
Intestinal Epithelial Cells ◽  
Delivery Mode ◽  
Apparent Difference ◽  
Intestinal Epithelial ◽  
Microbial Composition ◽  
Delivery Modes ◽  
Rat Pups ◽  
Stool Samples

BackgroundThe infant’s intestine contains diverse microbiota, which play an important role in an infant’s health.ObjectiveThis study aimed to analyze the different intestinal microbiota and their function in two delivery modes [vaginal delivery and cesarean section (C-section)] and to investigate the proprieties of bacteria associated with vaginal delivery on the development of intestinal epithelial cells in rat pups.Materials and MethodsWe evaluated the intestinal microbial diversity of the stool samples of 51 infants of subjects who underwent vaginal delivery and C-section by sequencing the V4 regions of the 16S rRNA gene and predicted the function of the microbiotas. The infant stool microbiota in the vaginal delivery group was associated with the digestive system and cell growth and death, whereas that of the C-section group was associated with membrane transport. Then, we isolated the strains based on function prediction.ResultsA total of 95 strains were isolated in the vaginal delivery group. Bifidobacterium bifidum FL-228.1 (FL-228.1) was screened and selected owing to its good surface hydrophobicity, bacterial survivability in the simulated gastrointestinal condition and adhesion ability to the IEC-6 cell line as well as owing to the development of intestinal epithelial cells. Furthermore, in vivo experiments revealed that FL-228.1 exhibited favorable effects on the development of intestinal epithelial cells in rat pups.ConclusionThe results of this study indicate an apparent difference in the bacterial composition of the stool samples collected from infants of the two delivery modes. By analyzing and screening the bacteria in infant stool samples, we found that one strain, i.e., B bifidum FL-228.1, exhibited favorable effects on the development of intestinal epithelial cells.

scholarly journals Effects of dietary fibers and prebiotics in adiposity regulation via modulation of gut microbiota

2020 ◽  
Vol 63 (1) ◽  
Author(s):  
Adriana Rivera-Piza ◽  
Sung-Joon Lee
Keyword(s):  
Metabolic Disease ◽  
Gut Microbiota ◽  
Metabolic Disorders ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Dietary Fibers ◽  
Intestinal Epithelial Barrier ◽  
Gut Homeostasis ◽  
Gut Microorganisms

AbstractThe microbiota is indispensable for human health and the regulation of various body functions, including energy metabolism. The harmonic crosstalk between the microbiota and the intestinal epithelial barrier determines gut homeostasis and health status in the healthy subject. Obesity and type 2 diabetes risk are, to some extent, explained by alterations in the microbiota. Since recent data indicate that the population of gut microorganisms can influence nutrient absorption and energy storage thus prevalence on obesity and metabolic disorders. Moreover, metabolic disease conditions, such as obesity, may be stimulated by genetic, environmental factors and by pathways that link metabolism with the immune system. On the basis of the above considerations, this review compiles the current results obtained in recent studies indicating the gut microbiota contribution to obesity development.

Sign in / Sign up

Export Citation Format

Share Document