A Study on Mesoporous Silica Loaded With Novel Photosensitizers HCE6 and Oxaliplatin for the Treatment of Cholangiocarcinoma

PurposeCholangiocarcinoma (CCA) is a malignant tumor with a high incidence. The therapeutic effect of conventional chemotherapy and radiotherapy is not obvious. Photodynamic therapy (PDT) is an ideal modality to fight cancer, and the nature of photosensitizer limits its application in clinical therapy. The aim of this study was to explore a novel mode of drug delivery for the intervention of bile duct cancer.MethodsOxaliplatin and photosensitizer HCE6 were loaded with mesoporous silica nanoparticles (MSNs) to synthesize Oxaliplatin/HCE6-MSNs (OH-MSNs); the structure of OH-MSNs was characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS), the drug release rate was detected by high performance liquid chromatography; the cellular activity, apoptosis level, and the expression levels of intracellular apoptosis and autophagy-related factors of OH-MSNs on cholangiocarcinoma cells were observed by CCK-8, flow cytometry, colony formation assay, and Western blot; the effects of OH-MSNs on cholangioma growth were observed by mouse tumor formation, immunohistochemistry, and tissue Tunel staining.ResultsThe release of OH-MSNs to Oxaliplatin was enhanced under acidic conditions; compared with Oxaliplatin or O-MSNs, OH-MSNs showed more potent killing effects against cholangiocarcinoma cells (P<0.05), and exerted notably inhibitory effects on the activity of cholangiocarcinoma cells (P<0.05), promoted their apoptosis (P<0.05), and greatly facilitated the expression of pro-apoptotic factors and autophagic factors in cholangiocarcinoma cells (P<0.05), and markedly inhibited the expression of anti-apoptotic factors and autophagic inhibitory factors (P<0.05); moreover, OH-MSNs could significantly suppress the growth of mouse cholangiocarcinoma (P<0.05) and induce apoptosis of tumor cells compared with Oxaliplatin or O-MSNs (P<0.05).ConclusionMSNs loading greatly increases the killing effect of Oxaliplatin on cholangiocarcinoma cells and upgrades the autophagic level of cholangiocarcinoma cells, while OH-MSNs synthesized by further loading HCE6 have a more apparent killing effect on cholangiocarcinoma cells.

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Amine-Functionalized Mesoporous Silica Nanoparticles: A New Nanoantibiotic for Bone Infection Treatment

Biomedical glasses ◽

10.1515/bglass-2017-0011 ◽

2017 ◽

Vol 3 (1) ◽

Cited By ~ 1

Author(s):

Daniel Pedraza ◽

Jaime Díez ◽

Isabel Izquierdo-Barba ◽

Montserrat Colilla ◽

María Vallet-Regí

Keyword(s):

Mesoporous Silica ◽

Silica Nanoparticles ◽

Mesoporous Silica Nanoparticles ◽

Chemical Properties ◽

Bone Infection ◽

Bacterial Biofilm ◽

Functionalized Mesoporous Silica ◽

Transmission Electron ◽

Infection Treatment ◽

Physical And Chemical

AbstractThis manuscript reports an effective new alternative for the management of bone infection by the development of an antibiotic nanocarrier able to penetrate bacterial biofilm, thus enhancing antimicrobial effectiveness. This nanosystem, also denoted as “nanoantibiotic”, consists in mesoporous silica nanoparticles (MSNs) loaded with an antimicrobial agent (levofloxacin, LEVO) and externally functionalized with N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (DAMO) as targeting agent. This amine functionalization provides MSNs of positive charges, which improves the affinity towards the negatively charged bacteria wall and biofilm. Physical and chemical properties of the nanoantibiotic were studied using different characterization techniques, including Xray diffraction (XRD), transmission electron microscopy (TEM), N

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Evaluating biological activity of folic acid-modified and 10-hydroxycamptothecin-loaded mesoporous silica nanoparticles

10.22541/au.163364394.48960944/v1 ◽

2021 ◽

Author(s):

Mei-Xia Zhao ◽

Di-Feng Chen ◽

Xue-Jie Zhao ◽

Lin-Song Li ◽

Yong-Fang Liu

Keyword(s):

Folic Acid ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Folate Receptor ◽

Mesoporous Silica Nanoparticles ◽

Drug Carriers ◽

Transmission Electron ◽

Before And After ◽

Drug Efficiency

Targeted nanocarrier can selectively deliver anti-tumor drugs to cancer sites improving drug efficiency. Accordingly, a targeted nanocarrier (MSN-FA) was synthesized based on folic acid (FA) modified mesoporous silica nanoparticles (MSNs). These loaded with 10-hydroxycamptothecin (HCPT) to obtain the nano-drug MSN-FA@HCPT. These nanocarriers were characterized by transmission electron microscopy (TEM), zeta potential, ultraviolet-visible spectroscopy (UV-Vis), fourier transform infrared spectroscopy (FT-IR), and thermogravimetric analysis (TGA). Notably, the nanocarriers were nearly spherical before and after loading HCPT and exhibited good dispersibility. Also, folate receptor (FR) over-expressing HeLa cells and FR deficient HepG2 cells were used to evaluate in vitro cellular uptake and cytotoxicity of MSN-FA@HCPT and MSN@HCPT. Interestingly, FA-modified nanocarriers enhanced the cytotoxicity of HCPT by improving drug targeting to tumor cells. Also, apoptotic and mitochondrial membrane potential (MMP) reducing effects of MSN-FA@HCPT were more prominent than the MSNs without FA modification. MSN-FA@HCPT can be excellent drug carriers with profound biomedical applications.

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Investigating Methylene Blue Removal from Aqueous Solution by Cysteine-Functionalized Mesoporous Silica

Journal of Chemistry ◽

10.1155/2021/8839864 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Abeer M. Beagan

Keyword(s):

Electron Microscopy ◽

Aqueous Solution ◽

Methylene Blue ◽

Mesoporous Silica ◽

Mesoporous Silica Nanoparticles ◽

Influential Factors ◽

Functionalized Mesoporous Silica ◽

Transmission Electron ◽

Pseudo Second Order ◽

The Stöber Method

In this study, mesoporous silica nanoparticles (MSNs) were synthesised using the Stober method and functionalised with cysteine (MSN-Cys) for removal of Methylene Blue (MB) from aqueous solution using the batch method. The adsorbent nanoparticles were characterised by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), FTIR, BET, and TGA. Several influential factors on the adsorption of MB onto the surface of MSN-Cys particles were investigated, including pH, initial concentration, and contact time. The adsorption capacity of MB from aqueous solution increased from circa 70 mg/g MSN-Cys in acidic media to circa 140 mg/g MSN-Cys in basic media. Adsorption isotherms and kinetic models of adsorption were used to clarify the adsorption process. The measured adsorption isotherm was fitted with a Freundlich model for all solutions, and the kinetic model was determined to be pseudo-second-order.

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Co/Zn-Grafted Mesoporous Silica Nanoparticles Catalyzed Cyclohexanimine Oxidation under UV Irradiation: High Performance Ozonation Process

Silicon ◽

10.1007/s12633-020-00464-8 ◽

2020 ◽

Cited By ~ 1

Author(s):

Homa Choopan Tayefe ◽

Mohammad Reza Sazegar ◽

Ali Mahmoudi ◽

Khosrow Jadidi

Keyword(s):

Mesoporous Silica ◽

Silica Nanoparticles ◽

Uv Irradiation ◽

High Performance ◽

Mesoporous Silica Nanoparticles ◽

Ozonation Process

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Synthesis and Characterization of Thiol- and Carboxyl-Functionalized Mesoporous Silica Nanoparticles

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1142.220 ◽

2017 ◽

Vol 1142 ◽

pp. 220-224 ◽

Cited By ~ 1

Author(s):

Wan Xia Wang ◽

Yu Wang ◽

Hua Meng Gong ◽

Hong Hao Sun ◽

Ming Xing Liu

Keyword(s):

Electron Microscopy ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Mesoporous Silica Nanoparticles ◽

Spherical Shape ◽

Synthesis And Characterization ◽

Functionalized Mesoporous Silica ◽

Transmission Electron ◽

Ft Ir

The purpose of this article is to synthesize the thiol-and carboxyl-bifunctionalized mesoporous silica nanoparticles (CMS-SH-COOH). CMS-SH-COOH was successfully synthesized by co-condensation and post-grafting methods. Moreover, the particle size and structural properties of CMS-SH-COOH were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and Fourier transform infrared spectroscopy (FT-IR). The obtained results indicated that the CMS-SH-COOH presented a uniform spherical shape with a wormhole arrangement of the mesopores and a relatively narrow paticle distribition. Therefore, the CMS-SH-COOH might be a great potential carrier for the drug delivery system in the future.

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Synthesis and Characterization of Mesoporous Silica Nanoparticles Embedded Gadolinium Oxide

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.306-307.1275 ◽

2011 ◽

Vol 306-307 ◽

pp. 1275-1279 ◽

Cited By ~ 2

Author(s):

Ning Qi Luo ◽

Zhan Yun Huang ◽

Ping Luo ◽

Yuan Zhi Shao ◽

Di Hu Chen

Keyword(s):

Mesoporous Silica ◽

Self Assembly ◽

Mesoporous Silica Nanoparticles ◽

Sol Gel ◽

Mesoporous Structure ◽

Gadolinium Oxide ◽

X Ray Diffraction ◽

Transmission Electron ◽

The Mean

We first synthesized gadolinium oxide (Gd2O3) by a modified “polyol” strategy and then embedded it into mesoporous silica by a simple self-assembly sol-gel reaction. Scanning electron microscope (SEM) results show that the samples have good sphericity and good dispersibility. The structure of mesoporous silica was characterized by transmission electron microscopy (TEM) and small-angle X-ray diffraction (SAXRD). Results show that the mesoporous structure has not been destroyed after gadolinium oxide imbedding. The ratio of gadolinium and silica was determined by the mean of energy dispersive spectroscopy (EDS).

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Polyelectrolyte/mesoporous silica hybrid materials for the high performance multiple-detection of pH value and temperature

Polymer Chemistry ◽

10.1039/c5py00210a ◽

2015 ◽

Vol 6 (18) ◽

pp. 3529-3536 ◽

Cited By ~ 28

Author(s):

Feng Chen ◽

Xiaoping Jiang ◽

Tairong Kuang ◽

Lingqian Chang ◽

Dajiong Fu ◽

...

Keyword(s):

Mesoporous Silica ◽

Silica Nanoparticles ◽

Hybrid Materials ◽

High Performance ◽

Ph Value ◽

Mesoporous Silica Nanoparticles ◽

Multiple Detection ◽

Silica Hybrid

Mesoporous silica nanoparticles are modified by electrolyte polymer, giving significant ionic permselectivity under pH and/or temperature switches.

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Reducing Microbiological Adhesion on Aluminum by Using Silver Nanocrystals Encapsulated in Mesoporous Silica Nanoparticles

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.236-238.1775 ◽

2011 ◽

Vol 236-238 ◽

pp. 1775-1778 ◽

Cited By ~ 1

Author(s):

Li Hua Dong ◽

Tao Liu ◽

Li Zhang ◽

Yan Sheng Yin

Keyword(s):

Electron Microscopy ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Marine Bacterium ◽

Mesoporous Silica Nanoparticles ◽

Plate Count ◽

X Ray Diffraction ◽

Transmission Electron ◽

Bacterial Growth Curve ◽

Plate Count Method

In the present study, we report the preparation of silver nanocrystals encapsulated in mesoporous silica nanoparticles (Ag-MSN) with increased stability and enhanced anti-bacterial potency. The morphology of the nanoparticles was characterized by scanning electron microscopy and transmission electron microscopy. X-ray diffraction analyses proved that the silver nanocrystals were truly embedded in the mesoporous silica nanoparticles. The antibacterial effect of silver nanoparticles was proved by plate count method and bacterial growth curve. Moreover, the marine bacterium V. natriegens showed weak attachment to the aluminum coated with Ag -MSN. The mechanism of reducing microbiological adhesion was also discussed.

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