scholarly journals Increased Levels of Plasma Extracellular Heat-Shock Proteins 60 and 70 kDa Characterized Early-Onset Neonatal Sepsis

2021 ◽  
Vol 9 ◽  
Author(s):  
Arturo Alejandro Canul-Euan ◽  
Gibran Zúñiga-González ◽  
Janelly Estefania Palacios-Luna ◽  
Rolando Maida-Claros ◽  
Néstor Fabián Díaz ◽  
...  
Keyword(s):  
Heat Shock ◽  
Neonatal Sepsis ◽  
Predictive Value ◽  
Early Onset ◽  
C Reactive Protein ◽  
Hsp 70 ◽  
Blood Samples ◽  
Reactive Protein ◽  
Hsp 27 ◽  
Shock Proteins

Background: Extracellular heat-shock proteins (eHsp) are highly conserved molecules that play an important role in inflammatory diseases and have been quantified in plasma from patients with infectious diseases, including sepsis. There is a constant search for dependable biochemical markers that, in combination with conventional methods, could deliver a prompt and reliable diagnosis of early-onset neonatal sepsis.Objective: We sought to assess the level of eHsp-27, eHsp-60, eHsp-70, and tumor necrosis factor-alpha (TNFα) in plasma of healthy neonates at term and infants with early-onset neonatal sepsis.Methods: This study included 34 newborns that were classified as healthy neonates at term (blood samples from the umbilical cord, n = 23) or infants with early-onset neonatal sepsis (blood samples obtained from umbilical artery by standard sterile procedures before starting a systemic antibiotic intervention, n = 11). All blood samples were centrifuged, and the plasma recovered to determine eHsp-27, eHsp-60, eHsp-70, and TNFα levels by ELISA.Results: Our results indicate that the level of eHsp-27 in healthy neonates at term was 0.045 ± 0.024 pg/ml. This value decreased 2.5-fold in infants with early-onset neonate sepsis (0.019 ± 0.006 pg/ml, p = 0.004). In contrast, the levels of eHsp-60 and eHsp-70 in healthy neonates at term were 13.69 ± 5.3 and 4.03 ± 2.6 pg/ml, respectively. These protein levels increased significantly 1.8- and 1.9-fold in the plasma of infants with early-onset neonatal sepsis (p ≤ 0.001). The level of TNFα in healthy neonates at term was 2.94 ± 0.46 pg/ml, with a 3.0-fold increase in infants with early-onset neonatal sepsis (8.96 ± 0.72 pm/ml, p ≤ 0.001). The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of eHsp compared with that of C-reactive protein were 73.3, 60.0, 47.8, and 33.3%, respectively.Conclusion: This study demonstrated a consistent increase of eHsp-60 and eHsp-70 in the plasma of infants diagnosed with early-onset neonatal sepsis. These proteins showed higher sensitivity and specificity than C-reactive protein and blood culture test.

scholarly journals VALIDITY OF C- REACTIVE PROTEIN IN DIAGNOSIS OF NEONATAL SEPSIS.

2020 ◽  
pp. 1-4
Author(s):  
Ghongade P. G. ◽  
Khaire P. B.
Keyword(s):  
Birth Weight ◽  
Neonatal Sepsis ◽  
Predictive Value ◽  
Screening Test ◽  
Treatment Strategies ◽  
High Sensitivity ◽  
C Reactive Protein ◽  
Suspected Sepsis ◽  
Adequate Treatment ◽  
Reactive Protein

Background: Neonatal sepsis with its high incidence &grave prognosis, in spite of adequate treatment with modern antibiotics, has been a challenge for all times. Optimal diagnosis and treatment strategies are difficult to define. It is essential to diagnose early with laboratory investigation like serial CRP; so that a feasible, rapid and a relatively economic method to diagnose neonatal sepsis at earliest can be instituted even at basic health care level. hence a study was planned to find out the role of CRP against blood culture in early detection of neonatal sepsis. Aim & Objective: To evaluate Validity of C-Reactive Protein as a screening test in neonatal sepsis. Material and Method: This prospective study was carried out inpaediatric dept of medical college. 100 neonates (≤ 28 days) with suspected neonatal sepsis having a birth weight of ≥ 1000 grams admitted during a period from January 2020 to March 2020 were screened primarily with C-Reactive Protein. Serial level of CRPon the day of admission,2nd ,4th ,6th ,8th& 10th day was compared with the serial blood cultureon the day of admission,8th,15th& 21st day to establish the validity of CRP as a screening test.Data analysis carried out by Percentages, Chi Square test, Sensitivity, Specificity, Positive predictive value, Negative predictive value. Results: Amongst 100neonate 76% were early neonates,65% were low birth weight,CRP was having high sensitivity & specificity(78.57%,76.74% respectively). ROC analysis showed AUC 0.8 with p<0.001.Conclusion: CRP is a good screening test & establishes its validity in diagnosing suspected sepsis.

scholarly journals Relationship between Maternal Serum C-Reactive Protein, Funisitis and Early-Onset Neonatal Sepsis

2012 ◽  
Vol 27 (6) ◽  
pp. 674 ◽  
Author(s):  
Sung Youn Lee ◽  
Kyo Hoon Park ◽  
Eun Ha Jeong ◽  
Kyung Joon Oh ◽  
Aeli Ryu ◽  
...  
Keyword(s):  
Neonatal Sepsis ◽  
Early Onset ◽  
Maternal Serum ◽  
C Reactive Protein ◽  
Reactive Protein

Interaction of Plasminogen and Angiostatin with Heat Shock Proteins.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 1622-1622
Author(s):  
Anil K. Dudani ◽  
Jelica Mehic ◽  
Anthony Martyres
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Dependent Manner ◽  
Hsp 70 ◽  
Molar Excess ◽  
Hsp 27 ◽  
Human Plasminogen ◽  
Shock Proteins ◽  
Dose Dependent ◽  
Mcf 7

Abstract Previous studies from this laboratory have demonstrated that plasminogen and angiostatin bind to endothelial cell (EC) surface-associated actin via their kringles in a specific manner. Heat shock proteins (hsps) like hsp 27 are constitutively expressed by vascular ECs and regulate actin polymerization, cell growth and migration. Since many hsps have also been found to be highly abundant on cell surfaces and there is evidence that bacterial surface hsps may interact with human plasminogen, the purpose of this study was to determine whether human plasminogen and angiostatin would interact with human hsps. ELISAs were developed in our laboratory to assess these interactions. It was observed that plasminogen bound to hsps 27, 60 and 70. In all cases, binding was inhibited (85–90%) by excess (50 mM) lysine indicating kringle involvement. Angiostatin predominantly bound to hsp 27 and to hsp 70 in a concentration- and kringle-dependent manner. As observed previously for actin, there was dose-dependent inhibition of angiostatin’s interaction with hsp 27 by plasminogen. In addition, thirty-fold molar excess actin inhibited (up to 50%), the interaction of plasminogen with all hsps. However, thirty-fold molar excess actin could only inhibit the interaction of angiostatin with hsp 27 by 15–20%. FACS analyses indicated the presence of hsps 27, 60 and 70 on the surface of MCF-7 breast cancer cells but not on human umbilical vein ECs. Polyclonal antibodies to hsp 27 significantly inhibited the interaction of plasminogen and angiostatin with MCF-7 surface-associated hsp27 in a dose-dependent manner. Collectively, these data indicate that while plasminogen interacts specifically with hsp 27, 60 and 70, angiostatin interacts predominantly with hsp 27 and to some extent with hsp 70; plasminogen only partially displaces angiostatins binding to hsp 27; actin only partially displaces plasminogen/angiostatin binding to hsps and surface-associated hsp 27 can mediate the binding of both plasminogen and angiostatin to MCF-7 cells.

Diagnostic Value of Urine sTREM-1 and Urine C-reactive Protein for Infants with Late Onset Neonatal Sepsis

2019 ◽  
Vol 15 (02) ◽  
pp. 072-078
Author(s):  
Senem Alkan Ozdemir ◽  
Ruya Colak ◽  
Ezgi Yangin Ergon ◽  
Sebnem Calkavur
Keyword(s):  
Neonatal Sepsis ◽  
Predictive Value ◽  
Late Onset ◽  
Diagnostic Value ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Late Onset Sepsis ◽  
Noninvasive Markers ◽  
Serum Crp ◽  
Sepsis Detection

Abstract Objective Noninvasive markers have been increasingly used as a diagnostic marker for sepsis detection and monitoring of the disease. The aim of this observational, prospective pilot study was to investigate the diagnostic performance of urinary soluble triggering receptor expressed on myeloid cells (sTREM-1) and urine C-reactive protein (CRP) levels in the late onset neonatal sepsis and to compare them with serum CRP levels. Materials and Methods Sixty-six infants with clinical sepsis were included. Urine sTREM-1 and urine CRP were collected at the diagnosis of late-onset sepsis. All laboratory investigations were also noted from the infants. Results There were no significant differences between characteristics of the infants. Culture-positive neonates had significantly higher urine sTREM-1 than culture-negative neonates (p < 0.001). Using a cut-off point for urine sTREM-1 of 129 pg/mL, the sensitivity was 0.63, the specificity was 0.84, positive predictive value was 0.80, negative predictive value was 0.70. Urine sTREM-1 and urine CRP were recollected on the seventh day of sepsis treatment and it was found that the levels of sTREM-1 and CRP decreased. Conclusion This is the first study in the literature which evaluates the place of urine sTREM-1 and urine CRP in the diagnosis of neonatal sepsis. Urine sTREM-1 and urine CRP may be useful in the diagnosis of sepsis and in evaluating the effect of antibiotic treatment.

scholarly journals An Update on the Use of C-Reactive Protein in Early-Onset Neonatal Sepsis: Current Insights and New Tasks

Neonatology ◽  
2012 ◽  
Vol 102 (1) ◽  
pp. 25-36 ◽  
Author(s):  
Nora Hofer ◽  
Eva Zacharias ◽  
Wilhelm Müller ◽  
Bernhard Resch
Keyword(s):  
Neonatal Sepsis ◽  
Early Onset ◽  
C Reactive Protein ◽  
Reactive Protein

scholarly journals DIAGNOSTIC VALUE OF SIMULTANEOUS MEASUREMENT OF PROCALCITONIN, INTERLEUKIN-6 AND HS CRP IN PREDICTION OF EARLY-ONSET NEONATAL SEPSIS

2012 ◽  
Vol 4 (1) ◽  
pp. e2012028 ◽  
Author(s):  
Alireza Abdollahi ◽  
Saeed Shoar ◽  
Fatemeh Nayyeri ◽  
Mamak Shariat
Keyword(s):  
Interleukin 6 ◽  
Simultaneous Measurement ◽  
Neonatal Sepsis ◽  
Early Onset ◽  
Clinical Signs ◽  
C Reactive Protein ◽  
Maternal Risk ◽  
Reactive Protein ◽  
Early Onset Sepsis ◽  
Hs Crp

Neonatal sepsis is a major cause of morbidities and mortalities mostly remarkable in the third world nations .We aimed to assess the value of simultaneous measurement of procalcitonin (PCT) and interleukin-6 (IL-6) in association with high sensitive- C reactive protein in prediction of early neonatal sepsis.We performed a follow- up study on 95 neonates who were below 12 hours (h) of age, had clinical signs of sepsis or maternal risk factors for sepsis. Neonates were assigned to 4 groups including “proven early-onset sepsis”, “clinical early-onset sepsis”, “negative infectious status”, and “uncertain infectious status”. Blood samples were obtained within the first 12 h of birth repeated between 24 hours and 36 hours of age for determination of serum levels of PCT, IL-6, high sensitivie- C Reactive Protein (hs-CRP), and white blood cell (WBC) count.On admission, neonates with sepsis had a higher WBC count, IL-6, PCT, and hs-CRP levels compared with those neonates without sepsis. This remained significant even after 12-24 hours of admission. Also, patients with clinical evidences of sepsis had a higher serum level of PCT and IL-6 within 12-24 hours after admission compared to the patients with uncertain sepsis. In final The combination of IL-6, hs-CRP, and PCT seems to be predictive in diagnosis of early onset neonatal sepsis.

Procalcitonin and Neonatal Sepsis: Is This the Biomarker We Are Looking For?

2017 ◽  
Vol 36 (6) ◽  
pp. 380-384 ◽  
Author(s):  
Susan Givens Bell
Keyword(s):  
Neonatal Sepsis ◽  
Early Onset ◽  
Diagnostic Marker ◽  
C Reactive Protein ◽  
Neonatal Deaths ◽  
Live Births ◽  
Neonatal Disease ◽  
Protein Levels ◽  
Reactive Protein ◽  
Early Onset Sepsis

AbstractResearchers estimate the incidence of early onset sepsis as 0.77–1/1,000 live births. It remains as one of the leading causes of neonatal deaths. Clinicians and researchers continue to search for biomarkers for specific neonatal disease processes. Clinicians frequently trend C-reactive protein levels during evaluation for neonatal sepsis. Recently, researchers have begun to explore procalcitonin as a potentially useful diagnostic marker for neonatal sepsis.

scholarly journals Predictive Value of C-Reactive Protein-to-Albumin Ratio for Neonatal Sepsis

2021 ◽  
Vol Volume 14 ◽  
pp. 3207-3215
Author(s):  
Tiewei Li ◽  
Xiaojuan Li ◽  
Yulei Wei ◽  
Geng Dong ◽  
Jianwei Yang ◽  
...  
Keyword(s):  
Neonatal Sepsis ◽  
Predictive Value ◽  
C Reactive Protein ◽  
Albumin Ratio ◽  
Reactive Protein

scholarly journals Early Onset Neonatal Sepsis; Diagnostic Value of Some Laboratory Tests

2018 ◽  
Vol 4 (1) ◽  
pp. 1109-1114
Author(s):  
Tania Licona ◽  
German Fajardo ◽  
Rubén Ferrera ◽  
Alejandra Mazariegos
Keyword(s):  
Blood Culture ◽  
Neonatal Sepsis ◽  
Early Onset ◽  
Laboratory Tests ◽  
Calculated Data ◽  
Clinical Situation ◽  
Diagnostic Value ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Control Study

Early Onset Neonatal Sepsis (EONS) is a clinical situation resulting from the invasion and proliferation of bacteria, fungi or viruses in the newborn (NB) bloodstream, which occurs within the first 72 hours of life. To determine the diagnostic usefulness of laboratory tests performed on infants with suspicion of early neonatal sepsis at the Santa Barbara Integrated Hospital, Honduras. A case-control study was carried out during 2016; the cases were 20 infants with early onset neonatal sepsis, and the controls were 40 infants who were admitted as potentially septic, but the blood culture result was negative. Sensitivity, specificity, positive predictive value (PPV) and negative (NPV) of leukocytosis, platelets, initial C-reactive protein (CRP) and control were calculated. Data were analyzed with SPSS version 19. It was found that 17 (28.3 %) NB were women and 43 (71.7 %) were men. The VPP of the initial PCR was 5 %, increasing to 85 % in the control study. The isolated microorganism was enterobacter in 6 (30 %) of the RNs. Of the 23 (38.3 %) neonates who presented complications; 11 (48 %) had positive blood culture and 12 (52 %) had negative blood cultures. The discharge condition was medical discharge in 55 (92 %) and referred to a more complex hospital 5 (8 %) of the neonates. The VPP of the C-reactive protein increases considerably when doing a laboratory control,between 24-48 hours.

Sign in / Sign up

Export Citation Format

Share Document