Tristetraprolin Gene Single-Nucleotide Polymorphisms and mRNA Level in Patients With Rheumatoid Arthritis

To observe and evaluate the correlation between single-nucleotide polymorphisms (SNPs) and messenger RNA (mRNA) level related to tristetraprolin (TTP) in Chinese rheumatoid arthritis (RA). TapMan SNP was used for genotyping analysis in 580 RA patients and 554 healthy people. Association between TTP gene polymorphisms (rs251864 and rs3746083) and RA was obtained. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) technology was applied for the detection of TTP mRNA level in peripheral blood mononuclear cells (PBMCs) in 36 RA patients and 37 healthy people. We observed that the allele T of TTP rs3746083 increased RA susceptibility (p = 0.019). A significant difference was found under the dominant model of rs3746083 (p = 0.037). Further analysis showed the allele distribution of rs3746083 was nominally correlated with RF phenotype of RA patients (p = 0.045). Nevertheless, the association between TTP rs251864 and the incidence of RA was no statistically significant (p > 0.05). The TTP expression level in PBMCs of RA patients was significantly reduced (p < 0.001). In conclusion, the results of this experiment support that TTP may be involved in the pathogenesis of RA.

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Association between single-nucleotide polymorphisms in theSEC8L1 gene, which encodes a subunit of the exocyst complex, and rheumatoid arthritis in a Japanese population

Arthritis & Rheumatism ◽

10.1002/art.21013 ◽

2005 ◽

Vol 52 (5) ◽

pp. 1371-1380 ◽

Cited By ~ 14

Author(s):

Daisuke Hamada ◽

Yoichiro Takata ◽

Dai Osabe ◽

Kyoko Nomura ◽

Syuichi Shinohara ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Single Nucleotide Polymorphisms ◽

Japanese Population ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Exocyst Complex ◽

A Subunit

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Sex Differences in Circadian Clock Genes and Myocardial Infarction Susceptibility

Journal of Cardiovascular Development and Disease ◽

10.3390/jcdd8050053 ◽

2021 ◽

Vol 8 (5) ◽

pp. 53

Author(s):

Ivana Škrlec ◽

Jasminka Talapko ◽

Martina Juzbašić ◽

Robert Steiner

Keyword(s):

Myocardial Infarction ◽

Cardiovascular Disease ◽

Circadian Rhythm ◽

Single Nucleotide Polymorphisms ◽

Clock Genes ◽

P Value ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Biological Sex ◽

Significant Difference

The growing body of evidence shows a significant difference in the circadian rhythm of cardiovascular disease based on biological sex. The incidence of cardiovascular disease varies between women and men. Additionally, biological sex is vital for the timely application of therapy—chronotherapy, which benefits both sexes. This study aimed to examine the potential difference of single nucleotide polymorphisms (SNPs) of the circadian rhythm genes ARNTL, CLOCK, CRY2 and PER2 in women and men with myocardial infarction. A cross-sectional study was conducted, including 200 patients with myocardial infarction. Altogether, ten single nucleotide polymorphisms in the ARNTL, CLOCK, CRY2 and PER2 genes were analyzed. The Chi-square test yielded statistically significant differences in CLOCK gene rs11932595 polymorphism in a recessive genotype model between women and men with a p-value of 0.03 and an odds ratio 2.66, and a corresponding 95% confidence interval of 1.07 to 6.66. Other analyzed polymorphisms of the circadian rhythm genes ARNTL, CRY2, and PER2 did not significantly differ between the sexes. According to the study’s current results, the CLOCK gene’s genetic variability might affect myocardial infarction concerning biological sex.

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Identification of 142 single nucleotide polymorphisms in 41 candidate genes for rheumatoid arthritis in the Japanese population

Human Genetics ◽

10.1007/s004390051040 ◽

2000 ◽

Vol 106 (3) ◽

pp. 293-297 ◽

Cited By ~ 17

Author(s):

Y. Ohnishi ◽

K. Suematsu ◽

M. Minami ◽

T. Seki ◽

M. Yukioka ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Single Nucleotide Polymorphisms ◽

Candidate Genes ◽

Japanese Population ◽

Nucleotide Polymorphisms ◽

Single Nucleotide

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Two-stage approach for identifying single-nucleotide polymorphisms associated with rheumatoid arthritis using random forests and Bayesian networks

BMC Proceedings ◽

10.1186/1753-6561-1-s1-s56 ◽

2007 ◽

Vol 1 (S1) ◽

Cited By ~ 18

Author(s):

Yan Meng ◽

Qiong Yang ◽

Karen T Cuenco ◽

L Adrienne Cupples ◽

Anita L DeStefano ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Single Nucleotide Polymorphisms ◽

Bayesian Networks ◽

Random Forests ◽

Nucleotide Polymorphisms ◽

Two Stage ◽

Single Nucleotide

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Single nucleotide polymorphisms at the TRAF1/C5 locus are associated with rheumatoid arthritis in a Han Chinese population

BMC Medical Genetics ◽

10.1186/1471-2350-12-53 ◽

2011 ◽

Vol 12 (1) ◽

Cited By ~ 9

Author(s):

Jing Zhu ◽

Dinging Zhang ◽

Fengxia Wu ◽

Fei He ◽

Xiaoqi Liu ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Single Nucleotide Polymorphisms ◽

Chinese Population ◽

Han Chinese ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Han Chinese Population

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A study on associations of single-nucleotide polymorphisms within H19 and HOX transcript antisense RNA (HOTAIR) with genetic susceptibility to rheumatoid arthritis in a Chinese population

Inflammation Research ◽

10.1007/s00011-017-1035-5 ◽

2017 ◽

Vol 66 (6) ◽

pp. 515-521 ◽

Cited By ~ 6

Author(s):

Jian-Zhe Zhou ◽

Jing-Jing Li ◽

Dong-Jin Hua ◽

Si-Chao Huang ◽

Qing-Qing Sun ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Single Nucleotide Polymorphisms ◽

Genetic Susceptibility ◽

Antisense Rna ◽

Chinese Population ◽

Nucleotide Polymorphisms ◽

Single Nucleotide

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Genoset for CALCA and RAMP1 Single Nucleotide Polymorphisms Are Related to the Magnitude of Headache Symptoms After Concussion: A Preliminary Observation

Neurology ◽

10.1212/01.wnl.0000801948.78004.36 ◽

2021 ◽

Vol 98 (1 Supplement 1) ◽

pp. S22.3-S23

Author(s):

Michael F. La Fountaine ◽

Anthony Testa

Keyword(s):

Single Nucleotide Polymorphisms ◽

Saliva Sample ◽

Area Under The Curve ◽

Nucleotide Polymorphisms ◽

Blurred Vision ◽

Single Nucleotide ◽

Commercial Laboratory ◽

Pain Transmission ◽

Significant Difference ◽

A Prospective Study

ObjectiveDetermine whether single nucleotide polymorphisms (SNPs) of the calcitonin gene-related polypeptide (CGRP)-alpha (CALCA) and the receptor activity modifying protein-1 (RAMP1) are related to headache burden during the first week after concussion.BackgroundPost-traumatic headache is a commonly reported symptom after concussion. SNPs related to CGRP are involved in the pathogenesis of migraine headaches and contribute to pain transmission and neurogenic inflammation. It is unclear in concussed persons if the headache burden is associated with genetic variations related to CGRP.Design/MethodsA prospective study was performed in 34 concussed athletes (gender: 23 female, 11 male; age: 20 ± 1 years; height: 1.75 ± 0.12 meters; weight: 73 ± 14 kilograms). Participants completed the symptom evaluation checklist from the SCAT3 within 48 hours of injury (V1), and 4 (V2) and 7 (V3) days after injury. For each visit, the self-reported score (0–6) for headache, pressure in head, blurred vision, and sensitivity to light/noise were summed. The area under-the-curve (AUC) was computed for the early (EHB: V1 to V2) and late (LHB: V2 to V3) burden of headache-related symptoms. A saliva sample was obtained and a commercial laboratory identified the genotype for CALCA (rs3781719) and RAMP1 (rs10185142) using PMR-array. RAMP1 genotypes RAMP1 (TT, TC, CC) and CALCA (AA, AG, GG) genotypes were dichotomized (T+, T−, and A+, A− respectively) and concatenated (T + A+, T + A−, T−A+, T-A−) for analyses.ResultsA significant difference for EHB (p = 0.003, partial η2 = 0.417) was present across RAMP1+CALCA genotypes, but not for the LHB. The T + A+ subgroup had a significantly elevated EHB compared to the all-other subgroups (p < 0.05: T + A + [n = 16]: 31.6 ± 2.6; T + A − [n = 9]: 17.7 ± 3.6; T−A+ [n = 8]: 18.4 ± 3.7; T−A-[n = 1]: 0.0 ± 0.0). Gender served as a covariate and diagnosed concussion history had no impact.ConclusionsThe current analysis provides a proof-of-concept to suggest that the combined T + A+ genoset from RAMP1+CALCA are associated with a greater headache burden in the first 4 days after concussion injury.

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