Development and Identification of SSR Markers Associated with Starch Properties and β-Carotene Content in the Storage Root of Sweet Potato (Ipomoea batatas L.)

The present study evaluated selected East African (EA) sweetpotato varieties for storage root dry matter and nutrient content and obtained information on the potential contributions of the varieties to alleviate vitamin A and mineral deficiencies. Roots obtained from 89 farmer (white- and orange-fleshed) varieties and one introduced variety (‘Resisto’) were analyzed for storage root quality using near-infrared reflectance spectroscopy technology. Location differences were only significant for starch content. The variance was significant (P < 0.01) for all the traits except sucrose content. Overall, the farmer varieties had higher dry matter, higher starch, and lower sucrose contents than the control clone, ‘Resisto’. It is these qualities that make sweetpotato attractive as a starchy staple in EA. A low population's mean β-carotene content (19.0 ppm) was observed. However, deep orange-fleshed farmer varieties, ‘Carrot_C’, ‘Ejumula’, ‘Carrot Dar’, ‘Mayai’, and ‘Zambezi’, had β-carotene content that can meet 350% or greater of recommended daily allowance (RDA) with 250-g serving to a 5- to 8–year-old child. More but light orange-fleshed farmer varieties ‘K-118’, ‘K-134’, ‘K-46’, ‘KMI61’, ‘MLE162 Nakahi’, ‘PAL161’, ‘Sowola6’, ‘Sponge’, ‘SRT34 Abuket2’, ‘SRT35 Anyumel’, ‘SRT52’, and ‘Sudan’ can provide 50% to 90% RDA of pro-vitamin A for the child. The root minerals’ content was generally low except for magnesium whose content can meet 50% or greater RDA in many farmer varieties. However, in areas with high sweetpotato consumption, varieties ‘Carrot_C’, ‘Carrot Dar’, ‘KRE nylon’, ‘MLE163 Kyebandula’, and ‘SRT49 Sanyuzameza’ can make good intakes of iron, zinc, calcium, and magnesium. In conclusion, some EA farmer varieties can contribute greatly to alleviation of vitamin A deficiency and substantial mineral intakes.

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Orange-Fleshed Sweet Potato (Ipomoea batatas L (Lam)) Composite Spaghetti has Good Consumer Acceptability and a Significant Source of Dietary Vitamin A

10.46619/fpns.2020.1-1003 ◽

2020 ◽

Vol 1 (1) ◽

pp. 25-30

Author(s):

Atinpoore Atuna Richard ◽

◽

Deborah Nyarkoa ◽

Kweku Amagloh Francis ◽

◽

...

Keyword(s):

Vitamin A ◽

Sweet Potato ◽

Wheat Flour ◽

Ipomoea Batatas ◽

Dietary Vitamin ◽

Consumer Acceptability ◽

Carotene Content ◽

Overall Acceptability ◽

Orange Fleshed Sweet Potato ◽

Β Carotene

Objective In this study, orange-fleshed sweet potato (OFSP) puree was substituted with wheat flour (48:52%) in the production of spaghetti, herein denoted VitAspag and compared with three existing whole wheat brands (Local, Oba and Spaghetti). Methods Hundred untrained panel list evaluated the colour, taste, stickiness, firmness and overall acceptability using a 5-point hedonic scale. The proximate and β-carotene content of VitAspag was carried out using standard methods and compared with the most preferred existing brand, Spaghetti. Results The sensory scores for all spaghetti brands were above 3, an indication of good consumer acceptability. There was no significant (p>0.05) difference among vitAspag, Local, Oba and Spaghetti with respect to all sensory attributes considered. The moisture, ash and fibre content ranged from 8.90% - 9.50%, 1.15% - 1.20% and 0.42% - 0.62%, respectively. Spaghetti, had a significantly higher protein (2.02% vs. 0.05%; p<0.001) and carbohydrates (81.0% vs. 79.7%; p = 0.024) content compared with VitAspag. VitAspag, was also significantly (p<0.001) higher in β-carotene, almost 1.6 times higher than Spaghetti. A 125 g and 500 g of vitAspag per day will respectively meet 10% and 9.03% of the daily vitamin A requirement of children <5 years (300 µg RAE/day) and pregnant and lactating mothers (1,300 µg RAE/day). Conclusion Ranking VitAspag similar to the existing commercial brand is an indication that consumers will equally accept it. OFSP puree could be composited with wheat flour up to (48:52%) on as is basis to make spaghetti and will contribute to a significant amount of dietary intake of vitamin A.

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Changes in β-carotene Content of Thermally Processed Sweet Potato (Ipomoea batatas) Cultivar

Journal of Applied Life Sciences International ◽

10.9734/jalsi/2016/22265 ◽

2016 ◽

Vol 5 (1) ◽

pp. 1-7

Author(s):

A Ukom ◽

P Ojimelukwe

Keyword(s):

Sweet Potato ◽

Ipomoea Batatas ◽

Carotene Content ◽

Thermally Processed ◽

Β Carotene

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Potassium Fertilization Stimulates Sucrose-to-Starch Conversion and Root Formation in Sweet Potato (Ipomoea batatas (L.) Lam.)

International Journal of Molecular Sciences ◽

10.3390/ijms22094826 ◽

2021 ◽

Vol 22 (9) ◽

pp. 4826

Author(s):

Yang Gao ◽

Zhonghou Tang ◽

Houqiang Xia ◽

Minfei Sheng ◽

Ming Liu ◽

...

Keyword(s):

Sweet Potato ◽

Ipomoea Batatas ◽

Intercellular Co2 Concentration ◽

Biomass Accumulation ◽

Starch Accumulation ◽

Storage Root ◽

Root Yield ◽

Starch Conversion ◽

And Storage ◽

Low K

A field experiment was established to study sweet potato growth, starch dynamic accumulation, key enzymes and gene transcription in the sucrose-to-starch conversion and their relationships under six K2O rates using Ningzishu 1 (sensitive to low-K) and Xushu 32 (tolerant to low-K). The results indicated that K application significantly improved the biomass accumulation of plant and storage root, although treatments at high levels of K, i.e., 300–375 kg K2O ha−1, significantly decreased plant biomass and storage root yield. Compared with the no-K treatment, K application enhanced the biomass accumulation of plant and storage root by 3–47% and 13–45%, respectively, through promoting the biomass accumulation rate. Additionally, K application also enhanced the photosynthetic capacity of sweet potato. In this study, low stomatal conductance and net photosynthetic rate (Pn) accompanied with decreased intercellular CO2 concentration were observed in the no-K treatment at 35 DAT, indicating that Pn was reduced mainly due to stomatal limitation; at 55 DAT, reduced Pn in the no-K treatment was caused by non-stomatal factors. Compared with the no-K treatment, the content of sucrose, amylose and amylopectin decreased by 9–34%, 9–23% and 6–19%, respectively, but starch accumulation increased by 11–21% under K supply. The activities of sucrose synthetase (SuSy), adenosine-diphosphate-glucose pyrophosphorylase (AGPase), starch synthase (SSS) and the transcription of Susy, AGP, SSS34 and SSS67 were enhanced by K application and had positive relationships with starch accumulation. Therefore, K application promoted starch accumulation and storage root yield through regulating the activities and genes transcription of SuSy, AGPase and SSS in the sucrose-to-starch conversion.

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Heterosis and combining ability for storage root, flesh color, virus disease resistance and vine weight in Sweet potato [Ipomoea batatas (L.) Lam.]

African Journal of Agricultural Research ◽

10.5897/ajar2019.14498 ◽

2020 ◽

Vol 15 (2) ◽

pp. 187-202

Author(s):

Ba Aliou ◽

C. Gemenet Dorcus ◽

Onguso Justus ◽

Diouf Diaga ◽

Mendes Thiago ◽

...

Keyword(s):

Disease Resistance ◽

Sweet Potato ◽

Combining Ability ◽

Ipomoea Batatas ◽

Virus Disease ◽

Storage Root ◽

Flesh Color

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Genetic Variation and Sequence Diversity of Starch Biosynthesis and Sucrose Metabolism Genes in Sweet Potato

Agronomy ◽

10.3390/agronomy10050627 ◽

2020 ◽

Vol 10 (5) ◽

pp. 627

Author(s):

Kai Zhang ◽

Kai Luo ◽

Shixi Li ◽

Deliang Peng ◽

Daobin Tang ◽

...

Keyword(s):

Sweet Potato ◽

Ipomoea Batatas ◽

Molecular Mechanisms ◽

High Throughput Sequencing ◽

Genetic Variations ◽

Starch Biosynthesis ◽

Sucrose Metabolism ◽

Gene Copy ◽

Starch Properties ◽

Sequence Variations

Knowledge of genetic variations can provide clues into the molecular mechanisms regulating key crop traits. Sweet potato (Ipomoea batatas (L.) Lam.) is an important starch-producing crop, but little is known about the genetic variations in starch biosynthesis and sucrose metabolism genes. Here, we used high-throughput sequencing of pooled amplicons of target genes to identify sequence variations in 20 genes encoding key enzymes involved in starch biosynthesis and sucrose metabolism in 507 sweet potato germplasms. After filtering potential variations between gene copies within the genome, we identified 622 potential allelic single nucleotide polymorphisms (SNPs) and 85 insertions/deletions (InDels), including 50 non-synonymous SNPs (nsSNPs) and 12 frameshift InDels. Three nsSNPs were confirmed to be present in eight sweet potato varieties with various starch properties using cleaved amplified polymorphic sequence (CAPS) markers. Gene copy with loss of the fifth intron was detected in IbAGPb3 genes, and loss of multiple introns were observed in IbGBSS1-1 genes and various among germplasms based on intron length polymorphism (ILP) markers. Thus, we identified sequence variations between germplasms in 20 genes involved in starch biosynthesis and sucrose metabolism, and demonstrated the diversity in intron-loss alleles among sweet potato germplasms. These findings provide critical genetic information and useful molecular markers for revealing regulatory mechanism of starch properties.

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