scholarly journals Lignification of Sheepgrass Internodes at Different Developmental Stages and Associated Alteration of Cell Wall Saccharification Efficiency

2017 ◽  
Vol 8 ◽  
Author(s):  
Jianli Wang ◽  
Lichao Ma ◽  
Zhongbao Shen ◽  
Dequan Sun ◽  
Peng Zhong ◽  
...  
Keyword(s):  
Cell Wall ◽  
Developmental Stages ◽  
Saccharification Efficiency

scholarly journals Exploring the Use of Fruit Callus Culture as a Model System to Study Color Development and Cell Wall Remodeling during Strawberry Fruit Ripening

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 805
Author(s):  
Pablo Ric-Varas ◽  
Marta Barceló ◽  
Juan A. Rivera ◽  
Sergio Cerezo ◽  
Antonio J. Matas ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Lines ◽  
Developmental Stages ◽  
Callus Cultures ◽  
Cellulose Microfibrils ◽  
Minor Amount ◽  
Strawberry Fruit ◽  
Color Development ◽  
Cell Wall Remodeling ◽  
Matrix Glycans

Cell cultures derived from strawberry fruit at different developmental stages have been obtained to evaluate their potential use to study different aspects of strawberry ripening. Callus from leaf and cortical tissue of unripe-green, white, and mature-red strawberry fruits were induced in a medium supplemented with 11.3 µM 2,4-dichlorophenoxyacetic acid (2,4-D) under darkness. The transfer of the established callus from darkness to light induced the production of anthocyanin. The replacement of 2,4-D by abscisic acid (ABA) noticeably increased anthocyanin accumulation in green-fruit callus. Cell walls were isolated from the different fruit cell lines and from fruit receptacles at equivalent developmental stages and sequentially fractionated to obtain fractions enriched in soluble pectins, ester bound pectins, xyloglucans (XG), and matrix glycans tightly associated with cellulose microfibrils. These fractions were analyzed by cell wall carbohydrate microarrays. In fruit receptacle samples, pectins were abundant in all fractions, including those enriched in matrix glycans. The amount of pectin increased from green to white stage, and later these carbohydrates were solubilized in red fruit. Apparently, XG content was similar in white and red fruit, but the proportion of galactosylated XG increased in red fruit. Cell wall fractions from callus cultures were enriched in extensin and displayed a minor amount of pectins. Stronger signals of extensin Abs were detected in sodium carbonate fraction, suggesting that these proteins could be linked to pectins. Overall, the results obtained suggest that fruit cell lines could be used to analyze hormonal regulation of color development in strawberry but that the cell wall remodeling process associated with fruit softening might be masked by the high presence of extensin in callus cultures.

scholarly journals Relationship between sugarcane culm and leaf biomass composition and saccharification efficiency

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
K. Hodgson-Kratky ◽  
G. Papa ◽  
A. Rodriguez ◽  
V. Stavila ◽  
B. Simmons ◽  
...  
Keyword(s):  
Cell Wall ◽  
Ionic Liquid ◽  
Lignocellulosic Biomass ◽  
Plant Cell Wall ◽  
Biomass Composition ◽  
Leaf Biomass ◽  
Dilute Acid ◽  
Sugarcane Varieties ◽  
Saccharification Efficiency ◽  
G Ratio

Abstract Background Lignocellulosic biomass is recognized as a promising renewable feedstock for the production of biofuels. However, current methods for converting biomass into fermentable sugars are considered too expensive and inefficient due to the recalcitrance of the secondary cell wall. Biomass composition can be modified to create varieties that are efficiently broken down to release cell wall sugars. This study focused on identifying the key biomass components influencing plant cell wall recalcitrance that can be targeted for selection in sugarcane, an important and abundant source of biomass. Results Biomass composition and the amount of glucan converted into glucose after saccharification were measured in leaf and culm tissues from seven sugarcane genotypes varying in fiber composition after no pretreatment and dilute acid, hydrothermal and ionic liquid pretreatments. In extractives-free sugarcane leaf and culm tissue, glucan, xylan, acid-insoluble lignin (AIL) and acid-soluble lignin (ASL) ranged from 20 to 32%, 15% to 21%, 14% to 20% and 2% to 4%, respectively. The ratio of syringyl (S) to guaiacyl (G) content in the lignin ranged from 1.5 to 2.2 in the culm and from 0.65 to 1.1 in the leaf. Hydrothermal and dilute acid pretreatments predominantly reduced xylan content, while the ionic liquid (IL) pretreatment targeted AIL reduction. The amount of glucan converted into glucose after 26 h of pre-saccharification was highest after IL pretreatment (42% in culm and 63.5% in leaf) compared to the other pretreatments. Additionally, glucan conversion in leaf tissues was approximately 1.5-fold of that in culm tissues. Percent glucan conversion varied between genotypes but there was no genotype that was superior to all others across the pretreatment groups. Path analysis revealed that S/G ratio, AIL and xylan had the strongest negative associations with percent glucan conversion, while ASL and glucan content had strong positive influences. Conclusion To improve saccharification efficiency of lignocellulosic biomass, breeders should focus on reducing S/G ratio, xylan and AIL content and increasing ASL and glucan content. This will be key for the development of sugarcane varieties for bioenergy uses.

Acid phosphatase isoforms in dry seeds and during seedling development in barley (Hordeum vulgare)

1996 ◽  
Vol 74 (5) ◽  
pp. 653-658
Author(s):  
S. Pasqualini ◽  
P. Batini ◽  
L. Ederli ◽  
F. Panara ◽  
M. Antonielli
Keyword(s):  
Cell Wall ◽  
Acid Phosphatase ◽  
Hordeum Vulgare ◽  
Developmental Stages ◽  
Soluble Fraction ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seedling Development ◽  
Rapid Decrease ◽  
Hordeum Vulgare L ◽  
Electrophoretic Patterns

The acid phosphatase activity in the soluble, membrane, and cell wall fractions from Hordeum vulgare in dry seeds and during seedling development was investigated. The acid phosphatase activities were also assayed in barley roots and coleoptiles at different developmental stages. Electrophoretic patterns of multiple acid phosphatases in seeds, endosperms and embryos, and growing roots and coleoptiles are shown. The enzyme activity shows a rapid decrease in both roots and coleoptiles during growth. Using nondenaturing polyacrylamide gel electrophoresis, multiple acid phosphatase forms were found in all the organs examined. However, no qualitative differences in the location of bands were observed between root and coleoptile extract at various stages of development. The coleoptile cell wall fraction showed an acid phosphatase form characterized by a very low electrophoretic mobility that was not found in the soluble fraction. Keywords: barley, Hordeum vulgare L., acid phosphatase, isoforms, seedlings growth.

Assessment of Genetic Variability of Cell Wall Degradability for the Selection of Alfalfa with Improved Saccharification Efficiency

2012 ◽  
Vol 5 (4) ◽  
pp. 904-914 ◽  
Author(s):  
Marc-Olivier Duceppe ◽  
Annick Bertrand ◽  
Sivakumar Pattathil ◽  
Jeffrey Miller ◽  
Yves Castonguay ◽  
...  
Keyword(s):  
Cell Wall ◽  
Genetic Variability ◽  
Saccharification Efficiency ◽  
Cell Wall Degradability ◽  
Selection Of

scholarly journals Tomato Locule Cell Wall Polysaccharides: Changes during Maturation and Ripening

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 786B-786
Author(s):  
Guiwen W. Cheng ◽  
Donald J. Huber
Keyword(s):  
Cell Wall ◽  
Developmental Stages ◽  
Gel Filtration ◽  
Neutral Sugar ◽  
Cell Wall Polysaccharides ◽  
Extraction Solvent ◽  
Sds Page ◽  
Mole Percentage ◽  
Neutral Sugars ◽  
Locule Tissue

Softening and liquefaction of `Solar Set' locules was studied by examining cell wall polysaccharides during fruit developmental stages (FDS) of immature green, mature green and breaker. Ethanol insoluble solids (EIS) were sequentially extracted by H2O, CDTA, and Na2CO3 solutions. The chromatograms of gel filtration among the same-solution extracts of EISs from three FDS were similar. Gradient DEAE also yielded similar patterns among FDS in each extraction solvent, even though the patterns of Na2CO3 extracts differed from those of H2O and CDTA extracts. The mole ratio of total polyuronides decreased for Gal, Ara, and Xyl at later FDS in both EIS and in all extracted polymers. Gal had the highest mole percentage of total neutral sugars, followed by Ara, Xyl, and Rha. While the mole percentage of neutral sugars for Gal, Rha, Ara, and Xyl were relatively similar among FDS in H2O extracts, those in CDTA and Na2CO3 extracts either increased or decreased, depending on individual neutral sugar. SDS-PAGE showed increased density in locule-tissue proteins, especially one with a molecular weight of less than 20 kDa, during later FDS. Results indicate that pectin depolymerization was limited and major neutral sugars commonly composing side chains showed a net decrease.

scholarly journals Arabidopsis GELP7 is plasma membrane-localized acetyl xylan esterase, and its overexpression improves saccharification efficiency.

2021 ◽  
Author(s):  
Lavi Rastogi ◽  
Aniket Anant Chaudhari ◽  
Raunak Sharma ◽  
Prashant Pawar
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Secondary Cell Wall ◽  
Acetyl Xylan Esterase ◽  
Acetyl Content ◽  
Transgenic Lines ◽  
Saccharification Efficiency ◽  
Cellulose Digestibility ◽  
Pre Treatment ◽  
Post Synthesis

Abstract Acetyl substitution on the xylan chain is critical for stable interaction with cellulose and other cell wall polymers in the secondary cell wall. Xylan acetylation pattern is governed by Golgi and extracellular localized acetyl xylan esterase (AXE). We investigated the role of Arabidopsis clade Id from the GDSL esterase/lipase or GELP family in polysaccharide deacetylation. The investigation of the AtGELP7 T-DNA mutant line showed a decrease in stem esterase activity and an increase in stem acetyl content. We further generated overexpressor AtGELP7 transgenic lines, and these lines showed a decrease in xylan acetylation in comparison with wild type plants. Therefore, we have named this enzyme as AtAXE1. The subcellular localization studies showed that the AtAXE1 enzyme is secreted out, associated with the plasma membrane and involved in xylan de-esterification post-synthesis. The cellulose digestibility was improved in AtAXE1 overexpressor lines without pre-treatment, after alkali and xylanases pre-treatment. Furthermore, we have also established that the AtGELP7 gene is upregulated in the overexpressor line of AtMYB46, which is a secondary cell wall specific transcription factor. This transcriptional regulation can drive AtGELP7 or AtAXE1 to perform de-esterification of xylan in a tissue-specific manner.

Changes in cell wall composition during ripening of grape berries

2020 ◽  
Author(s):  
KJ Nunan ◽  
Ian Sims ◽  
A Bacic ◽  
SP Robinson ◽  
GB Fincher
Keyword(s):  
Cell Wall ◽  
Developmental Stages ◽  
Protein Composition ◽  
Fold Increase ◽  
Vitis Vinifera L ◽  
Type I ◽  
Cell Wall Polysaccharide ◽  
Pectic Polysaccharides ◽  
Grape Berries ◽  
Specific Polysaccharide

Cell walls were isolated from the mesocarp of grape (Vitis vinifera L.) berries at developmental stages from before veraison through to the final ripe berry. Fluorescence and light microscopy of intact berries revealed no measurable change in cell wall thickness as the mesocarp cells expanded in the ripening fruit. Isolated walls were analyzed for their protein contents and amino acid compositions, and for changes in the composition and solubility of constituent polysaccharides during development. Increases in protein content after veraison were accompanied by an approximate 3-fold increase in hydroxyproline content. The type I arabinogalactan content of the pectic polysaccharides decreased from approximately 20 mol % of total wall polysaccharides to about 4 mol % of wall polysaccharides during berry development. Galacturonan content increased from 26 to 41 mol % of wall polysaccharides, and the galacturonan appeared to become more soluble as ripening progressed. After an initial decrease in the degree of esterification of pectic polysaccharides, no further changes were observed nor were there large variations in cellulose (30-35 mol % of wall polysaccharides) or xyloglucan (approximately 10 mol % of wall polysaccharides) contents. Overall, the results indicate that no major changes in cell wall polysaccharide composition occurred during softening of ripening grape berries, but that significant modification of specific polysaccharide components were observed, together with large changes in protein composition.

scholarly journals Stability of Cell Wall Composition and Saccharification Efficiency in Miscanthus across Diverse Environments

2017 ◽  
Vol 7 ◽  
Author(s):  
Tim van der Weijde ◽  
Oene Dolstra ◽  
Richard G. F. Visser ◽  
Luisa M. Trindade
Keyword(s):  
Cell Wall ◽  
Cell Wall Composition ◽  
Saccharification Efficiency
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