Assessment of Genetic Variability of Cell Wall Degradability for the Selection of Alfalfa with Improved Saccharification Efficiency

2012 ◽  
Vol 5 (4) ◽  
pp. 904-914 ◽  
Author(s):  
Marc-Olivier Duceppe ◽  
Annick Bertrand ◽  
Sivakumar Pattathil ◽  
Jeffrey Miller ◽  
Yves Castonguay ◽  
...  
2014 ◽  
Vol 14 (2) ◽  
pp. 94-101 ◽  
Author(s):  
Sonia Maria Lima Salgado ◽  
Juliana Costa de Rezende ◽  
José Airton Rodrigues Nunes

The purpose of this study was to select Coffea arabica progenies for resistance to M. paranaensis in an infested coffee growing area using Henderson's mixed model methodology. Forty-one genotypes were selected at the Coffee Active Germplasm Bank of Minas Gerais, and evaluated in regard to stem diameter, number of plagiotropic branches, reaction to the nematode, and yield per plant. There was genetic variability among the genotypes studied for all the traits evaluated, and among the populations studied for yield and reaction to the nematode, indicating possibilities for obtaining genetic gains through selection in this population. There was high rate of genotypic association between all the traits studied. Coffee plants of Timor Hybrid UFV408-01 population, and F3 progenies derived from crossing Catuaí Vermelho and Amphillo MR 2161 were the most promising in the area infested by M. paranaensis.


2019 ◽  
Vol 59 (7) ◽  
pp. 85-91
Author(s):  
Yulia A. Smyatskaya ◽  
◽  
Natalia A. Politaeva ◽  
Amira Toumi ◽  
◽  
...  

This article discusses the effect of the disintegration of the cell wall of the microalgae Chlorella sorokiniana on the output of the lipid fraction. The biomass of the microalgae Chlorella sorokiniana was grown under laboratory conditions in special photobioreactors at a temperature of 25 °C, with a constant aeration of a mixture of carbon dioxide and air at a rate of 1.5 liters/min, illumination 2200-2800 Lx. Nutrient medium for cultivation contained macro – and micronutrients for high-speed growth of microalgae. Selection of optimal cultivation parameters allows obtaining biomass with desired properties. Disintegration was carried out with the homogenization of biomass and under the influence of microwave radiation. Extraction of lipids was carried out on a semi-automatic extractor according to the Randall method, using organic solvents. The output of the lipid fraction without treatment was 10.18% after the destruction of the cell wall 14.45% with the homogenization of biomass and 13.85% under the influence of microwave radiation. A qualitative analysis of the lipid fraction, carried out under gas chromatography, obtained under various conditions showed that there was no significant difference in composition from the disintegration method. Lipid fractions (more than 50%) in both cases consist mainly of unsaturated fatty acids, of which irreplaceable unsaturated fatty acids constitute more than 18% for both samples. The residual biomass formed after the extraction of the lipid fraction can be used as fertilizer in the plant, for the manufacture of sorption materials for the purification of industrial water and as a biofuel. The purpose of this study was to study the effect of cell wall disintegration on the output of the lipid fraction and qualitative composition.


2020 ◽  
Vol 5 (01) ◽  
pp. 45-49
Author(s):  
Ankit Kumar ◽  
Amit Tomar

The results revealed that parents namely, TSK-10, TSK-27, New Blue-II, Kurara and TSK-109 were found highly genetic diverse for days to 50% tasseling, days to 50% silking, days to 755 dry husk. The parents namely, TSK-109, Kurara, New Blue-II and TSK-10 were found highly genetic diverse for plant height (cm), cob height, number of cobs per plant and number of grains per cob. The parents namely, Kurara, TSK-109, TSK-10, New Blue-II and TSK-27 were found highly genetic diverse for shelling percentage, grain yield per plant, grain yield per cob and 100-grain weight.


2019 ◽  
Vol 12 (1) ◽  
Author(s):  
K. Hodgson-Kratky ◽  
G. Papa ◽  
A. Rodriguez ◽  
V. Stavila ◽  
B. Simmons ◽  
...  

Abstract Background Lignocellulosic biomass is recognized as a promising renewable feedstock for the production of biofuels. However, current methods for converting biomass into fermentable sugars are considered too expensive and inefficient due to the recalcitrance of the secondary cell wall. Biomass composition can be modified to create varieties that are efficiently broken down to release cell wall sugars. This study focused on identifying the key biomass components influencing plant cell wall recalcitrance that can be targeted for selection in sugarcane, an important and abundant source of biomass. Results Biomass composition and the amount of glucan converted into glucose after saccharification were measured in leaf and culm tissues from seven sugarcane genotypes varying in fiber composition after no pretreatment and dilute acid, hydrothermal and ionic liquid pretreatments. In extractives-free sugarcane leaf and culm tissue, glucan, xylan, acid-insoluble lignin (AIL) and acid-soluble lignin (ASL) ranged from 20 to 32%, 15% to 21%, 14% to 20% and 2% to 4%, respectively. The ratio of syringyl (S) to guaiacyl (G) content in the lignin ranged from 1.5 to 2.2 in the culm and from 0.65 to 1.1 in the leaf. Hydrothermal and dilute acid pretreatments predominantly reduced xylan content, while the ionic liquid (IL) pretreatment targeted AIL reduction. The amount of glucan converted into glucose after 26 h of pre-saccharification was highest after IL pretreatment (42% in culm and 63.5% in leaf) compared to the other pretreatments. Additionally, glucan conversion in leaf tissues was approximately 1.5-fold of that in culm tissues. Percent glucan conversion varied between genotypes but there was no genotype that was superior to all others across the pretreatment groups. Path analysis revealed that S/G ratio, AIL and xylan had the strongest negative associations with percent glucan conversion, while ASL and glucan content had strong positive influences. Conclusion To improve saccharification efficiency of lignocellulosic biomass, breeders should focus on reducing S/G ratio, xylan and AIL content and increasing ASL and glucan content. This will be key for the development of sugarcane varieties for bioenergy uses.


Crop Science ◽  
2006 ◽  
Vol 46 (4) ◽  
pp. 1801-1809 ◽  
Author(s):  
H. G. Jung ◽  
M. D. Casler

2019 ◽  
Vol 97 (7) ◽  
pp. 2769-2779 ◽  
Author(s):  
Michelle M Judge ◽  
Thierry Pabiou ◽  
Jessica Murphy ◽  
Stephen B Conroy ◽  
P J Hegarty ◽  
...  

Abstract The ability to alter the morphology of cattle towards greater yields of higher value primal cuts has the potential to increase the value of animals at slaughter. Using weight records of 14 primal cuts from 31,827 cattle, the objective of the present study was to quantify the extent of genetic variability in these primal cuts; also of interest was the degree of genetic variability in the primal cuts adjusted to a common carcass weight. Variance components were estimated for each primal cut using animal linear mixed models. The coefficient of genetic variation in the different primal cuts ranged from 0.05 (bavette) to 0.10 (eye of round) with a mean coefficient of genetic variation of 0.07. When phenotypically adjusted to a common carcass weight, the coefficient of genetic variation of the primal cuts was lesser ranging from 0.02 to 0.07 with a mean of 0.04. The heritability of the 14 primal cuts ranged from 0.14 (bavette) to 0.75 (topside) with a mean heritability across all cuts of 0.48; the heritability estimates reduced, and ranged from 0.12 (bavette) to 0.56 (topside), when differences in carcass weight were accounted for in the statistical model. Genetic correlations between each primal cut and carcass weight were all ≥0.77; genetic correlations between each primal cut and carcass conformation score were, on average, 0.59 but when adjusted to a common carcass weight, the correlations weakened to, on average, 0.27. The genetic correlations among all 14 primal cut weights was, on average, strong (mean correlation of 0.72 with all correlations being ≥0.37); when adjusted to a common carcass weight, the mean of the genetic correlations among all primal cuts was 0.10. The ability of estimated breeding values for a selection of primal cuts to stratify animals phenotypically on the respective cut weight was demonstrated; the weight of the rump, striploin, and fillet of animals estimated to be in the top 25% genetically for the respective cut, were 10 to 24%, 12 to 24%, and 7 to 17% heavier than the weight of cuts from animals predicted to be in the worst 25% genetically for that cut. Significant exploitable genetic variability in primal carcass cuts was clearly evident even when adjusted to a common carcass weight. The high heritability of many of the primal cuts infers that large datasets are not actually required to achieve high accuracy of selection once the structure of the data and the number of progeny per sire is adequate.


2019 ◽  
Vol 81 (2) ◽  
pp. 87-104
Author(s):  
Sabrin Sultana ◽  
Firoz Mahmud ◽  
Md Abdur Rahim

Sesame (Sesamum indicum L.) is one of the oldest oilseed crops and important for high nutritional quality as well as medicinal value. Fifty diverse sesame genotypes were evaluated to study genetic variability. The results revealed that the genotypes were a significant variation in most of the studied characters. In all cases, the phenotypic variances were much higher than genotypic variances suggests a higher level of the environmental effect on the expression of these characters. The highest genotypic coefficient of variations (GCV) was observed in seed yield per plant while the highest heritability was exhibited by hundred seed weight followed by days to 80% maturity, pods per plant, number of branches per plant and seed yield per plant. The genotypic correlation with seed yield per plant showed a significantly strong positive with days to 50% flowering, plant height and number of pods per plant at both the genotypic and phenotypic level. The path coefficient analysis showed that pods per plant and seeds per pod were the most important contributing traits to seed yield. The 50 sesame genotypes were grouped into five clusters. The highest inter-cluster distance was observed between the cluster III and V while the lowest inter-cluster distance was observed between the cluster III and IV. Among 50 sesame genotypes G7, G36, G38 and G46 might be suggested for future hybridization program for the improvement of sesame yield.


2020 ◽  
Vol 133 (19) ◽  
pp. jcs254649

ABSTRACTFirst Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Destiny Davis is first author on ‘Callose deposition is essential for the completion of cytokinesis in the unicellular alga Penium margaritaceum’, published in JCS. Destiny conducted the research described in this article while a PhD student in Georgia Drakakaki's lab at University of California, Davis, CA, USA. She is now a postdoc in the lab of Jenny Mortimer at the Joint BioEnergy Institute (JBEI), Emeryville, CA, USA, where she is endlessly fascinated by the inner workings of the plant cell related to the cell wall.


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