Ascorbic Acid-A Potential Oxidant Scavenger and Its Role in Plant Development and Abiotic Stress Tolerance

Humic acid (HA) is a principal component of humic substances, which make up the complex organic matter that broadly exists in soil environments. HA promotes plant development as well as stress tolerance, however the precise molecular mechanism for these is little known. Here we conducted transcriptome analysis to elucidate the molecular mechanisms by which HA enhances salt stress tolerance. Gene Ontology Enrichment Analysis pointed to the involvement of diverse abiotic stress-related genes encoding HEAT-SHOCK PROTEINs and redox proteins, which were up-regulated by HA regardless of salt stress. Genes related to biotic stress and secondary metabolic process were mainly down-regulated by HA. In addition, HA up-regulated genes encoding transcription factors (TFs) involved in plant development as well as abiotic stress tolerance, and down-regulated TF genes involved in secondary metabolic processes. Our transcriptome information provided here provides molecular evidences and improves our understanding of how HA confers tolerance to salinity stress in plants.

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Seasonal changes in abiotic stress tolerance and concentrations of tocopherol, sugar, and ascorbic acid in sea buckthorn leaves and stems

Scientia Horticulturae ◽

10.1016/j.scienta.2013.09.039 ◽

2013 ◽

Vol 164 ◽

pp. 232-237 ◽

Cited By ~ 13

Author(s):

Yoshinori Kanayama ◽

Kazuyoshi Sato ◽

Hiroki Ikeda ◽

Tomoko Tamura ◽

Manabu Nishiyama ◽

...

Keyword(s):

Ascorbic Acid ◽

Abiotic Stress ◽

Stress Tolerance ◽

Seasonal Changes ◽

Abiotic Stress Tolerance ◽

Sea Buckthorn

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Genome-wide analysis of Myo-inositol oxygenase gene family in tomato reveals their involvement in ascorbic acid accumulation

10.21203/rs.2.14506/v1 ◽

2019 ◽

Author(s):

Shoaib Munir ◽

Muhammad Ali Mumtaz ◽

John Kojo Ahiakpa ◽

Genzhong Liu ◽

Wei Zheng ◽

...

Keyword(s):

Ascorbic Acid ◽

Abiotic Stress ◽

Gene Family ◽

Stress Tolerance ◽

Abiotic Stress Tolerance ◽

Light Response ◽

Genome Wide ◽

Genes Encoding ◽

Transgenic Lines ◽

Distribution Mapping

Abstract Background Ascorbic acid (Vitamin C, AsA) is an antioxidant metabolite involved in plant development and environmental stimuli. AsA biosynthesis has been well studied in plants and MIOX is a critical enzyme in plants AsA biosynthesis pathway. However, myo-inositol oxygenase (MIOX) gene family members and their involvement in AsA biosynthesis and response to abiotic stress remain unclear. Results In this study, five tomato genes encoding MIOX proteins and possessing MIOX motifs were identified. Structural analysis and distribution mapping showed that 5 MIOX genes contain different intron/exon patterns and unevenly distributed among four chromosomes. In addition, expression analyses indicated the remarkable expression of SlMIOX genes in different plant tissues. Furthermore, transgenic lines were obtained by over-expression of MIOX4 gene in tomato. The overexpression lines showed a significant increase in total ascorbate in leaves and red fruits compared to control. Expression analysis revealed that increased accumulation of AsA in MIOX4 overexpression lines is possible as a consequence of the multiple genes involved in AsA biosynthesis. Myo inositol (MI) feeding in leaf and fruit implied that AsA biosynthesis was mainly improved by Myo-inositol pathway in leaves and fruits. MIOX4 overexpression lines exhibited a betterlight response, abiotic stress tolerance, and AsA biosynthesis capacity. Conclusions These results showed that MIOX4 transgenic lines contribute to AsA biosynthesis, evident as better light response and improved oxidative stress tolerance. This study provides the first comprehensive analysis of MIOX gene family and their involvement in ascorbate biosynthesis in tomato.

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Exogenous Ascorbic Acid Mediated Abiotic Stress Tolerance in Plants

Ascorbic Acid in Plant Growth, Development and Stress Tolerance ◽

10.1007/978-3-319-74057-7_9 ◽

2017 ◽

pp. 233-253 ◽

Cited By ~ 1

Author(s):

Yi Xu ◽

Bingru Huang

Keyword(s):

Ascorbic Acid ◽

Abiotic Stress ◽

Stress Tolerance ◽

Abiotic Stress Tolerance

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Molecular cloning and functional characterization of a soybean GmGMP1 gene reveals its involvement in ascorbic acid biosynthesis and multiple abiotic stress tolerance in transgenic plants

Journal of Integrative Agriculture ◽

10.1016/s2095-3119(17)61727-1 ◽

2018 ◽

Vol 17 (3) ◽

pp. 539-553 ◽

Cited By ~ 2

Author(s):

Chen-chen XUE ◽

Jin-yan XU ◽

Can WANG ◽

Na GUO ◽

Jin-feng HOU ◽

...

Keyword(s):

Ascorbic Acid ◽

Abiotic Stress ◽

Transgenic Plants ◽

Stress Tolerance ◽

Molecular Cloning ◽

Abiotic Stress Tolerance ◽

Functional Characterization ◽

Acid Biosynthesis ◽

Ascorbic Acid Biosynthesis

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Genome-wide analysis of Myo-inositol oxygenase gene family in tomato reveals their involvement in ascorbic acid accumulation

10.21203/rs.2.14506/v3 ◽

2020 ◽

Author(s):

Shoaib Munir ◽

Muhammad Ali Mumtaz ◽

John Kojo Ahiakpa ◽

Genzhong Liu ◽

Weifang Chen ◽

...

Keyword(s):

Ascorbic Acid ◽

Abiotic Stress ◽

Gene Family ◽

Stress Tolerance ◽

Abiotic Stress Tolerance ◽

Light Response ◽

Genome Wide ◽

Genes Encoding ◽

Transgenic Lines ◽

Distribution Mapping

Abstract Background Ascorbic acid (Vitamin C, AsA) is an antioxidant metabolite involved in plant development and environmental stimuli. AsA biosynthesis has been well studied in plants, and MIOX is a critical enzyme in plants AsA biosynthesis pathway. However, Myo-inositol oxygenase (MIOX) gene family members and their involvement in AsA biosynthesis and response to abiotic stress remain unclear. Results In this study, five tomato genes encoding MIOX proteins and possessing MIOX motifs were identified. Structural analysis and distribution mapping showed that 5 MIOX genes contain different intron/exon patterns and unevenly distributed among four chromosomes. Besides, expression analyses indicated the remarkable expression of SlMIOX genes in different plant tissues. Furthermore, transgenic lines were obtained by over-expression of the MIOX4 gene in tomato. The overexpression lines showed a significant increase in total ascorbate in leaves and red fruits compared to control. Expression analysis revealed that increased accumulation of AsA in MIOX4 overexpression lines is possible as a consequence of the multiple genes involved in AsA biosynthesis. Myo inositol (MI) feeding in leaf and fruit implied that the Myo-inositol pathway improved the AsA biosynthesis in leaves and fruits. MIOX4 overexpression lines exhibited abetter light response, abiotic stress tolerance, and AsA biosynthesis capacity. Conclusions These results showed that MIOX4 transgenic lines contribute to AsA biosynthesis, evident as better light response and improved oxidative stress tolerance. This study provides the first comprehensive analysis of the MIOX gene family and their involvement in ascorbate biosynthesis in tomato.

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Lysine, Lysine-Rich, Serine, and Serine-Rich Proteins: Link Between Metabolism, Development, and Abiotic Stress Tolerance and the Role of ncRNAs in Their Regulation

Frontiers in Plant Science ◽

10.3389/fpls.2020.546213 ◽

2020 ◽

Vol 11 ◽

Author(s):

P. B. Kavi Kishor ◽

Renuka Suravajhala ◽

Guddimalli Rajasheker ◽

Nagaraju Marka ◽

Kondle Kavya Shridhar ◽

...

Keyword(s):

Abiotic Stress ◽

Stress Tolerance ◽

Plant Development ◽

Abiotic Stresses ◽

Abiotic Stress Tolerance ◽

Expression Patterns ◽

Critical Role ◽

Large Body ◽

Sr Proteins ◽

Plant Metabolism

Lysine (Lys) is indispensable nutritionally, and its levels in plants are modulated by both transcriptional and post-transcriptional control during plant ontogeny. Animal glutamate receptor homologs have been detected in plants, which may participate in several plant processes through the Lys catabolic products. Interestingly, a connection between Lys and serotonin metabolism has been established recently in rice. 2-Aminoadipate, a catabolic product of Lys appears to play a critical role between serotonin accumulation and the color of rice endosperm/grain. It has also been shown that expression of some lysine-methylated proteins and genes encoding lysine-methyltransferases (KMTs) are regulated by cadmium even as it is known that Lys biosynthesis and its degradation are modulated by novel mechanisms. Three complex pathways co-exist in plants for serine (Ser) biosynthesis, and the relative preponderance of each pathway in relation to plant development or abiotic stress tolerance are being unfolded slowly. But the phosphorylated pathway of L-Ser biosynthesis (PPSB) appears to play critical roles and is essential in plant metabolism and development. Ser, which participates indirectly in purine and pyrimidine biosynthesis and plays a pivotal role in plant metabolism and signaling. Also, L-Ser has been implicated in plant responses to both biotic and abiotic stresses. A large body of information implicates Lys-rich and serine/arginine-rich (SR) proteins in a very wide array of abiotic stresses. Interestingly, a link exists between Lys-rich K-segment and stress tolerance levels. It is of interest to note that abiotic stresses largely influence the expression patterns of SR proteins and also the alternative splicing (AS) patterns. We have checked if any lncRNAs form a cohort of differentially expressed genes from the publicly available PPSB, sequence read archives of NCBI GenBank. Finally, we discuss the link between Lys and Ser synthesis, catabolism, Lys-proteins, and SR proteins during plant development and their myriad roles in response to abiotic stresses.

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Genome-wide analysis of Myo-inositol oxygenase gene family in tomato reveals their involvement in ascorbic acid accumulation

10.21203/rs.2.14506/v2 ◽

2020 ◽

Author(s):

Shoaib Munir ◽

Muhammad Ali Mumtaz ◽

John Kojo Ahiakpa ◽

Genzhong Liu ◽

Wei Zheng ◽

...

Keyword(s):

Ascorbic Acid ◽

Abiotic Stress ◽

Gene Family ◽

Stress Tolerance ◽

Abiotic Stress Tolerance ◽

Light Response ◽

Genome Wide ◽

Genes Encoding ◽

Transgenic Lines ◽

Distribution Mapping

Abstract Background: Ascorbic acid (Vitamin C, AsA) is an antioxidant metabolite involved in plant development and environmental stimuli. AsA biosynthesis has been well studied in plants, and MIOX is a critical enzyme in plants AsA biosynthesis pathway. However, Myo-inositol oxygenase (MIOX) gene family members and their involvement in AsA biosynthesis and response to abiotic stress remain unclear. Results : In this study, five tomato genes encoding MIOX proteins and possessing MIOX motifs were identified. Structural analysis and distribution mapping showed that 5 MIOX genes contain different intron/exon patterns and unevenly distributed among four chromosomes. Besides, expression analyses indicated the remarkable expression of SlMIOX genes in different plant tissues. Furthermore, transgenic lines were obtained by over-expression of the MIOX4 gene in tomato. The overexpression lines showed a significant increase in total ascorbate in leaves and red fruits compared to control. Expression analysis revealed that increased accumulation of AsA in MIOX4 overexpression lines is possible as a consequence of the multiple genes involved in AsA biosynthesis. Myo inositol (MI) feeding in leaf and fruit implied that the Myo-inositol pathway improved the AsA biosynthesis in leaves and fruits. MIOX4 overexpression lines exhibited a better light response, abiotic stress tolerance, and AsA biosynthesis capacity. Conclusions: These results showed that MIOX4 transgenic lines contribute to AsA biosynthesis, evident as better light response and improved oxidative stress tolerance. This study provides the first comprehensive analysis of the MIOX gene family and their involvement in ascorbate biosynthesis in tomato.

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