Oryza sativa cv. Nipponbare and Oryza barthii as Unexpected Tolerance and Susceptibility Sources Against Schizotetranychus oryzae (Acari: Tetranychidae) Mite Infestation

Cultivated rice (Oryza sativa L.) is frequently exposed to multiple stresses, including Schizotetranychus oryzae mite infestation. Rice domestication has narrowed the genetic diversity of the species, leading to a wide susceptibility. This work aimed to analyze the response of two African rice species (Oryza barthii and Oryza glaberrima), weedy rice (O. sativa f. spontanea), and O. sativa cv. Nipponbare to S. oryzae infestation. Surprisingly, leaf damage, histochemistry, and chlorophyll concentration/fluorescence indicated that the African species present a higher level of leaf damage, increased accumulation of H2O2, and lower photosynthetic capacity when compared to O. sativa plants under infested conditions. Infestation decreased tiller number, except in Nipponbare, and caused the death of O. barthii and O. glaberrima plants during the reproductive stage. While infestation did not affect the weight of 1,000 grains in both O. sativa, the number of panicles per plant was affected only in O. sativa f. spontanea, and the percentage of full seeds per panicle and seed length were increased only in Nipponbare. Using proteomic analysis, we identified 195 differentially abundant proteins when comparing susceptible (O. barthii) and tolerant (Nipponbare) plants under control and infested conditions. O. barthii presents a less abundant antioxidant arsenal and is unable to modulate proteins involved in general metabolism and energy production under infested condition. Nipponbare presents high abundance of detoxification-related proteins, general metabolic processes, and energy production, suggesting that the primary metabolism is maintained more active compared to O. barthii under infested condition. Also, under infested conditions, Nipponbare presents higher levels of proline and a greater abundance of defense-related proteins, such as osmotin, ricin B-like lectin, and protease inhibitors (PIs). These differentially abundant proteins can be used as biotechnological tools in breeding programs aiming at increased tolerance to mite infestation.

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Nipponbare and wild rice species as unexpected tolerance and susceptibility sources against Schizotetranychus oryzae (Acari: Tetranychidae) mite infestation

10.1101/2020.01.22.914184 ◽

2020 ◽

Author(s):

Giseli Bufon ◽

Édina Aparecida dos Reis Blasi ◽

Thainá Inês Lamb ◽

Janete Mariza Adamski ◽

Joséli Schwambach ◽

...

Keyword(s):

Oryza Sativa ◽

Wild Rice ◽

Energy Production ◽

Leaf Damage ◽

Mite Infestation ◽

Oryza Glaberrima ◽

Primary Metabolism ◽

Wild Rice Species ◽

Related Proteins ◽

Differentially Abundant Proteins

AbstractCultivated rice (Oryza sativa L.) is frequently exposed to multiple stresses, including Schizotetranychus oryzae mite infestation. Rice domestication has narrowed the genetic diversity of the species, reducing the stress resistance and leading to a wide susceptibility. Therefore, wild rice species present an alternative to search for this lost variability. Aiming to observe the response of two wild rice species (Oryza barthii and Oryza glaberrima) and two Oryza sativa genotypes (cv. Nipponbare and O. sativa f. spontanea) to S. oryzae infestation, we used agronomic, physiological and molecular analyses. Surprisingly, analyses of leaf damage, histochemistry, chlorophyll concentration and chlorophyll fluorescence showed that the wild species present higher level of leaf damage, increased accumulation of H2O2 and lower photosynthetic capacity when compared to O. sativa genotypes under infested conditions. Infestation did not affect plant height, but decreased tiller number, except in cv. Nipponbare, whose development was not affected. Infestation also caused the death of wild plants during the reproductive stage, unlike O. sativa genotypes, which were able to tolerate stress and produce seeds. While infestation did not affect the weight of 1,000 grains in both O. sativa genotypes, the number of panicles per plant was affected only in O. sativa f. spontanea, and the percentage of full seeds per panicle and seed length were increased only in cv. Nipponbare. Proteomic analysis allowed us to identify 195 differentially abundant proteins when comparing susceptible (O. barthii) and tolerant (O. sativa cv. Nipponbare) genotypes under control and infested conditions. We found that O. barthii has a less abundant antioxidant arsenal. In addition, it is unable to modulate proteins involved with general metabolism and energy production under infested condition. In Nipponbare we found high abundance of detoxification-related proteins, general metabolic processes and energy production, which allows us to suggest that, under infested condition, the primary metabolism is maintained more active compared to O. barthii. Also, Nipponbare presents a greater abundance of defense-related proteins, such as osmotin, ricin B-like lectin, and protease inhibitors of the Bowman Birk trypsin inhibitor family, as well as higher levels of the compatible osmolyte Proline under infested condition. Identification of these differentially abundant proteins can be used as an important biotechnological tool in breeding programs that aim increased tolerance to phytophagous mite infestation.

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Allelic data revealing interrelatedness in rice species (Oryza sativa, Oryza glaberrima, Oryza barthii) and the interspecific hybrids (NERICA)

Journal of Plant Breeding and Crop Science ◽

10.5897/jpbcs10.054 ◽

2013 ◽

Vol 5 (2) ◽

pp. 12-19

Author(s):

R. Aliyu

Keyword(s):

Oryza Sativa ◽

Interspecific Hybrids ◽

Oryza Glaberrima ◽

Oryza Barthii

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Bioherbicidal Properties of Parthenium hysterophorus, Cleome rutidosperma and Borreria alata Extracts on Selected Crop and Weed Species

Agronomy ◽

10.3390/agronomy11040643 ◽

2021 ◽

Vol 11 (4) ◽

pp. 643

Author(s):

Mst. Motmainna ◽

Abdul Shukor Juraimi ◽

Md. Kamal Uddin ◽

Norhayu Binti Asib ◽

A. K. M. Mominul Islam ◽

...

Keyword(s):

Oryza Sativa ◽

Plant Extracts ◽

Oryza Sativa L ◽

Block Design ◽

Parthenium Hysterophorus ◽

Weedy Rice ◽

Test Plant ◽

Ageratum Conyzoides ◽

Natural Herbicide ◽

Cleome Rutidosperma

Natural product-based herbicides could be the effective alternatives to synthetic chemical herbicides for eco-friendly weed management. This research, therefore, was conducted to identify the phytotoxic properties of Parthenium hysterophorus L., Cleome rutidosperma DC. and Borreria alata (Aubl.) DC. with a view to introducing them as a tool for natural herbicide development. The methanol extracts of these plants were examined on the germination and growth of Zea mays L., Oryza sativa L., Abelmoschus esculentus (L.) Moench and Amaranthus gangeticus L., Oryza sativa f. Spontanea Roshev. (Weedy rice), Echinochloa colona (L.) Link., Euphorbia hirta L., and Ageratum conyzoides L. under laboratory and glasshouse conditions. A complete randomized design (CRD) with five replications and randomized complete block design (RCBD) with four replications were laid out for laboratory and glasshouse experiments, respectively. In the laboratory experiment, three plant extracts of 0, 6.25, 12.5, 50, and 100 g L−1 were tested on survival rate, hypocotyl, and radicle length of eight test plant species. No seed germination of A. conzyoides, E. hirta, and A. gangeticus were recorded when P. hysterophorus extract was applied at 50 g L−1. C. rutidosperma had the same effect on those plants at 100 g L−1. In the glasshouse, similar extracts and concentrations used in the laboratory experiments were sprayed on at the 2–3 leaf stage for grasses and 4–6 for the broadleaf species. Tested plants were less sensitive to C. rutidosperma and B. alata compared to P. hysterophorus extract. Among the weeds and crops, A. conyzoides, E. hirta, A. esculentus and A. gangeticus were mostly inhibited by P. hysterophorus extract at 100 g L−1. Based on these results, P. hysterophorus was the most phytotoxic among the tested plant extracts and could be used for developing a new natural herbicide for green agriculture.

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Agronomic practices and biotechnological methods dealing with the occurrence, dispersion, proliferation and adaptation of weedy rice (Oryza sativa f. spontanea)

International Journal of Pest Management ◽

10.1080/09670874.2021.1944697 ◽

2021 ◽

pp. 1-14

Author(s):

Serge Svizzero

Keyword(s):

Oryza Sativa ◽

Weedy Rice ◽

Agronomic Practices

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Physiological and Biochemical Responses of Ageratum conyzoides, Oryza sativa f. spontanea (Weedy Rice) and Cyperus iria to Parthenium hysterophorus Methanol Extract

Plants ◽

10.3390/plants10061205 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1205

Author(s):

Mst. Motmainna ◽

Abdul Shukor Juraimi ◽

Md. Kamal Uddin ◽

Norhayu Binti Asib ◽

A. K. M. Mominul Islam ◽

...

Keyword(s):

Oryza Sativa ◽

Methanol Extract ◽

Parthenium Hysterophorus ◽

Carotenoid Content ◽

Weedy Rice ◽

Ageratum Conyzoides ◽

Test Species ◽

Biochemical Responses ◽

Glasshouse Condition ◽

Physiological And Biochemical

The current study was designed to investigate the effect of Parthenium hysterophorus L. methanol extract on Ageratum conyzoides L., Oryza sativa f. spontanea (weedy rice) and Cyperus iria L. in glasshouse condition. Here, Parthenium hysterophorus methanol extract at 20, 40, and 60 g L−1 concentrations was applied on the test species to examine their physiological and biochemical responses at 6, 24, 48 and 72 h after spraying (HAS). The phytotoxicity of P. hysterophorus was strong on A. conyzoides compared to weedy rice and Cyperus iria at different concentrations and exposure times. There was a reduction in photosynthesis rate, stomatal conductance, transpiration, chlorophyll content and carotenoid content when plants were treated with P. hysterophorus extract concentrations. Exposure to P. hysterophorus (60 g L−1) at 24 HAS increased malondialdehyde (MDA) and proline content by 152% and 130%, respectively, in A. conyzoides compared with control. The activities of antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD)) were also increased in the presence of P. hysterophorus extract. Present findings confirm that the methanol extract of P. hysterophorus can disrupt the physiological and biochemical mechanism of target weeds and could be used as an alternative to chemical herbicides.

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Weedy Rice Diversity in Southern Brazil

Weed Science ◽

10.1017/wsc.2021.23 ◽

2021 ◽

pp. 1-37

Author(s):

Leonard Bonilla Piveta ◽

José Alberto Noldin ◽

Nilda Roma-Burgos ◽

Vívian Ebeling Viana ◽

Lariza Benedetti ◽

...

Keyword(s):

Oryza Sativa ◽

Flowering Time ◽

Weed Management ◽

Phenotypic Diversity ◽

Weedy Rice ◽

Phenotypic Traits ◽

Southern Brazil ◽

Variable Response ◽

Cultivated Rice ◽

Flowering Synchrony

Abstract Weedy rice (Oryza sativa L.) is one of the most troublesome weeds affecting rice (Oryza sativa L.) production in many countries. Weedy rice control is difficult in rice fields because the weed and crop are phenotypically and morphologically similar. Weedy rice can be a source of genetic diversity to cultivated rice. Thus, this study aimed to characterize the morphological diversity of weedy rice in Southern Brazil. Qualitative and quantitative traits of 249 accessions from eight rice growing mesoregions in Rio Grande do Sul (RS) and Santa Catarina (SC) states were analyzed. For each accession, 24 morphological descriptors (14 qualitative and 10 quantitative) were evaluated. All the 249 accessions from RS and SC are of indica lineage. Considering all the phenotypic traits evaluated, the accessions separated into 14 distinct groups. One of the largest groups consisted of plants that were predominantly tall and with green leaves, intermediate shattering, and variable in flowering time. Distinct subgroups exist within larger clusters, showing discernable phenotypic diversity within the main clusters. The variability in flowering time was high (77 to 110 d after emergence), indicating high potential for flowering synchrony with rice cultivars and, consequently, gene flow. This indicates the need to remove escapes when planting herbicide-resistant rice. Thus, weedy rice populations in Southern Brazil are highly diverse and this diversity could result in variable response to weed management.

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