scholarly journals Natural Genetic Diversity in Tomato Flavor Genes

2021 ◽  
Vol 12 ◽  
Author(s):  
Lara Pereira ◽  
Manoj Sapkota ◽  
Michael Alonge ◽  
Yi Zheng ◽  
Youjun Zhang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Solanum Lycopersicum ◽  
Fruit Quality ◽  
Tomato Fruit ◽  
Expression Patterns ◽  
High Genetic Diversity ◽  
Tomato Flavor ◽  
Main Determinants ◽  
Fruit Flavor ◽  
Genome Assemblies

Fruit flavor is defined as the perception of the food by the olfactory and gustatory systems, and is one of the main determinants of fruit quality. Tomato flavor is largely determined by the balance of sugars, acids and volatile compounds. Several genes controlling the levels of these metabolites in tomato fruit have been cloned, including LIN5, ALMT9, AAT1, CXE1, and LoxC. The aim of this study was to identify any association of these genes with trait variation and to describe the genetic diversity at these loci in the red-fruited tomato clade comprised of the wild ancestor Solanum pimpinellifolium, the semi-domesticated species Solanum lycopersicum cerasiforme and early domesticated Solanum lycopersicum. High genetic diversity was observed at these five loci, including novel haplotypes that could be incorporated into breeding programs to improve fruit quality of modern tomatoes. Using newly available high-quality genome assemblies, we assayed each gene for potential functional causative polymorphisms and resolved a duplication at the LoxC locus found in several wild and semi-domesticated accessions which caused lower accumulation of lipid derived volatiles. In addition, we explored gene expression of the five genes in nine phylogenetically diverse tomato accessions. In general, the expression patterns of these genes increased during fruit ripening but diverged between accessions without clear relationship between expression and metabolite levels.

scholarly journals Postharvest handling induces changes in fruit DNA methylation status and is associated with alterations in fruit quality in tomato (Solanum lycopersicum L.)

2020 ◽  
Author(s):  
Jiaqi Zhou ◽  
Bixuan Chen ◽  
Karin Albornoz ◽  
Diane M Beckles
Keyword(s):  
Dna Methylation ◽  
Solanum Lycopersicum ◽  
Fruit Quality ◽  
Fruit Ripening ◽  
Tomato Fruit ◽  
Transcriptional Analysis ◽  
Marker Genes ◽  
Tomato Fruit Ripening ◽  
Postharvest Handling ◽  
Early Harvest

AbstractPostharvest handling of tomato (Solanum lycopersicum L.), specifically low-temperature storage and early harvest are used to extend shelf life, but often reduce fruit quality. Recent work suggests that DNA methylation dynamics influences fruit ripening through the demethylase SlDML2 gene. However, the influence of postharvest handling on DNA methylation in relation to fruit quality is unclear. This work aimed to clarify these issues by analyzing DNA methylation using methyl-sensitive amplification polymorphism (MSAP), semi-quantitative transcriptional analysis of marker genes for fruit quality (RIN; RIPENING INHIBITOR) and DNA methylation (SlDML2; Solanum lycopersicum L. DNA demethylase 2), and, fruit biochemical quality biomarkers. Multivariate analysis of these data supported the view that DNA methylation of fruit was influenced more by postharvest handling than ripening stage, however, fruit quality was influenced mainly by ripening. Fruit chilled postharvest were distinct in their DNA methylation state and quality characteristics, which implied that these three phenomena i.e., chilling, methylation, and quality are highly connected. In addition, different postharvest handling methods modulated SlDML2 transcript levels but had little effect on the level of RIN transcripts in fruit that reached the Turning stage after early harvest, and cold storage. These data collectively helped to advance our interpretation of tomato fruit ripening. In conclusion, our findings revealed that postharvest-induced variation in fruit quality is in relation to DNA methylation. Long-term this work will help better connect physiological changes in tomato fruit to events happening at the molecular level.

scholarly journals EFFECT OF MICRONUTRIENTS AND PGR ON FRUIT QUALITY AND ECONOMICS OF TOMATO (SOLANUM LYCOPERSICUM L.) VARIETY AZAD-T6

2021 ◽  
Vol 21 (No 1) ◽  
Author(s):  
Vipul Pratap Singh ◽  
D. P. Singh ◽  
Bankey Lal ◽  
Mahendra Kumar Yadav ◽  
Mahendra Kumar Yadav ◽  
...  
Keyword(s):  
Boric Acid ◽  
Solanum Lycopersicum ◽  
Growth Regulators ◽  
Fruit Quality ◽  
Block Design ◽  
Tomato Fruit ◽  
Rabi Season ◽  
Solanum Lycopersicum L ◽  
Randomized Block Design

The present investigation was carried out during the rabi season 2018-19 and 2019-20 at Department of Vegetable Science Chandra Shekhar Azad University of Agriculture & Technology Kanpur U.P (208024) India. Experiment was laid out in randomized block design (RBD) with eleven treatments in replicated three times consist of two levels of each micronutrients and growth regulators with control i.e. T0 : Control, T1 : GA3 50ppm, T2 : GA3 100ppm, T3 : NAA 50ppm, T4 : NAA 100ppm, T5 : ZnSO4 0.5%, T6 : ZnSO4 1%, T7 : Boric acid 50ppm, T8 : Boric acid 100ppm, T9 : FeSO4 100ppm and T10: FeSO4 150ppm. Results revealed that there were significant differences between themicronutrients and PGR in tomato fruit quality. From the result it was observed that concentration of GA3 100ppm showed significant effect of fruit quality in tomato Azad-T6

scholarly journals Increasing the Sugar Concentration in Tomato Fruit Juice by Coiling Wire Around Plant Stems

2014 ◽  
Vol 24 (1) ◽  
pp. 76-80 ◽  
Author(s):  
Ken Takahata ◽  
Hiroyuki Miura
Keyword(s):  
Solanum Lycopersicum ◽  
Fruit Quality ◽  
Fruit Juice ◽  
Tomato Fruit ◽  
Leaf Node ◽  
Soluble Solids ◽  
Sugar Concentration ◽  
Moisture Condition ◽  
Control Fruit ◽  
Marketable Fruit

Sugar concentration is an important aspect of fruit quality in tomato (Solanum lycopersicum) production. Salt and water stresses have been reported to increase the sugar concentration of fruit. Coiling wire around the lower part of the stems to reduce the capacity of xylem to transport water to shoot would result in low shoot moisture condition and increase the sugar concentration of fruit like salt and water stresses. The objective of this study was to determine whether a prototypic method of wire coiling increases the sugar concentration of fruit. Bonsai wire was coiled around the stems of seedlings in February in a greenhouse between the cotyledon node and the first leaf node. Eleven days after treatment (DAT), the stem diameters immediately above the wire coils were markedly greater in treated plants compared with the corresponding stem regions of control plants. The stems of treated plants were less elongated and developed fewer nodes at 39 and 51 DAT than the control plants. Marketable fruit harvested from the first to third trusses of the treated plants in April to May had average weights that were 49% to 89% of the weights of fruit from control plants. The juice of fruit from the first to third trusses in the treated plants had soluble solids concentrations of 116% to 120%, sucrose concentrations of 263% to 483%, and fructose and glucose concentrations of 135% to 155%, compared with juice from corresponding control fruit. At 112 DAT, the shoots and roots of treated plants had weights that were 58% and 32% of those of control plants, respectively. The basal wire coiling treatment increased the sugar and soluble solids concentrations of tomato fruit juice; however, further investigation will be necessary before the technique can be adopted for practical use.

scholarly journals Subtype Characterization and Zoonotic Potential of Cryptosporidium felis in Cats in Guangdong and Shanghai, China

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 89
Author(s):  
Jiayu Li ◽  
Fuxian Yang ◽  
Ruobing Liang ◽  
Sheng Guo ◽  
Yaqiong Guo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Characterization ◽  
Zoonotic Potential ◽  
Common Occurrence ◽  
High Genetic Diversity ◽  
Gp60 Gene ◽  
The Common ◽  
Subtyping Tool

Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.

scholarly journals Influence of Bagging on the Development and Quality of Fruits

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 358
Author(s):  
Muhammad Moaaz Ali ◽  
Raheel Anwar ◽  
Ahmed F. Yousef ◽  
Binqi Li ◽  
Andrea Luvisi ◽  
...  
Keyword(s):  
Fruit Quality ◽  
Consumer Choice ◽  
Abiotic Factors ◽  
Market Value ◽  
Quality Attribute ◽  
Export Markets ◽  
External Appearance ◽  
The Usa ◽  
Main Determinants

Fruit quality is certainly influenced by biotic and abiotic factors, and a main quality attribute is the external appearance of the fruit. Various possible agronomical approaches are able to regulate the fruit microenvironment and, consequently, improve fruit quality and market value. Among these, fruit bagging has recently become an integral part of fruits’ domestic and export markets in countries such as Japan, China, Korea Australia and the USA because it is a safe and eco-friendly technique to protect fruits from multiple stresses, preserving or improving the overall quality. Despite increasing global importance, the development of suitable bagging materials and, above all, their use in the field is quite laborious, so that serious efforts are required to enhance and standardize bagging material according to the need of the crops/fruits. This review provides information about the effects of bagging technique on the fruit aspect and texture, which are the main determinants of consumer choice.

scholarly journals Phylogeography of the Brittle Star Ophiura sarsii Lütken, 1855 (Echinodermata: Ophiuroidea) from the Barents Sea and East Atlantic

Diversity ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 40
Author(s):  
Evgeny Genelt-Yanovskiy ◽  
Yixuan Li ◽  
Ekaterina Stratanenko ◽  
Natalia Zhuravleva ◽  
Natalia Strelkova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Barents Sea ◽  
Haplotype Diversity ◽  
Brittle Star ◽  
The Arctic ◽  
Coi Gene ◽  
High Genetic Diversity ◽  
Svalbard Archipelago ◽  
Mitochondrial Coi ◽  
The Barents Sea

Ophiura sarsii is a common brittle star species across the Arctic and Sub-Arctic regions of the Atlantic and the Pacific oceans. Ophiurasarsii is among the dominant echinoderms in the Barents Sea. We studied the genetic diversity of O.sarsii by sequencing the 548 bp fragment of the mitochondrial COI gene. Ophiurasarsii demonstrated high genetic diversity in the Barents Sea. Both major Atlantic mtDNA lineages were present in the Barents Sea and were evenly distributed between the northern waters around Svalbard archipelago and the southern part near Murmansk coast of Kola Peninsula. Both regions, and other parts of the O.sarsii range, were characterized by high haplotype diversity with a significant number of private haplotypes being mostly satellites to the two dominant haplotypes, each belonging to a different mtDNA clade. Demographic analyses indicated that the demographic and spatial expansion of O.sarsii in the Barents Sea most plausibly has started in the Bølling–Allerød interstadial during the deglaciation of the western margin of the Barents Sea.

scholarly journals Genetic Diversity of Leuconostoc mesenteroides Isolates from Traditional Montenegrin Brine Cheese

2021 ◽  
Vol 9 (8) ◽  
pp. 1612
Author(s):  
Werner Ruppitsch ◽  
Andjela Nisic ◽  
Patrick Hyden ◽  
Adriana Cabal ◽  
Jasmin Sucher ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Safety Evaluation ◽  
Gene Clusters ◽  
Northern Region ◽  
Genomic Diversity ◽  
High Genetic Diversity ◽  
Pathogenic Factors ◽  
Flavor Development ◽  
Genomic Characterization

In many dairy products, Leuconostoc spp. is a natural part of non-starter lactic acid bacteria (NSLAB) accounting for flavor development. However, data on the genomic diversity of Leuconostoc spp. isolates obtained from cheese are still scarce. The focus of this study was the genomic characterization of Leuconostoc spp. obtained from different traditional Montenegrin brine cheeses with the aim to explore their diversity and provide genetic information as a basis for the selection of strains for future cheese production. In 2019, sixteen Leuconostoc spp. isolates were obtained from white brine cheeses from nine different producers located in three municipalities in the northern region of Montenegro. All isolates were identified as Ln. mesenteroides. Classical multilocus sequence tying (MLST) and core genome (cg) MLST revealed a high diversity of the Montenegrin Ln. mesenteroides cheese isolates. All isolates carried genes of the bacteriocin biosynthetic gene clusters, eight out of 16 strains carried the citCDEFG operon, 14 carried butA, and all 16 isolates carried alsS and ilv, genes involved in forming important aromas and flavor compounds. Safety evaluation indicated that isolates carried no pathogenic factors and no virulence factors. In conclusion, Ln. mesenteroides isolates from Montenegrin traditional cheeses displayed a high genetic diversity and were unrelated to strains deposited in GenBank.

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