scholarly journals DNA Metabarcoding for Quality Control of Basil, Oregano, and Paprika

2021 ◽  
Vol 12 ◽  
Author(s):  
Ancuţa Cristina Raclariu-Manolică ◽  
Jarl Andreas Anmarkrud ◽  
Marcin Kierczak ◽  
Nima Rafati ◽  
Birgitte Lisbeth Graae Thorbek ◽  
...  
Keyword(s):  
Quality Control ◽  
Analytical Methods ◽  
False Positives ◽  
Food Sector ◽  
Constituent Species ◽  
Dna Metabarcoding ◽  
Product Authenticity ◽  
Marker Compounds ◽  
Analytical Approaches ◽  
Herbs And Spices

Herbs and spices are some of the most vulnerable products in terms of fraud and adulteration in the food sector. Although standard analytical methods are accurate for quality control of specific lead or marker compounds, they cannot accurately assess the entire species composition of many marketed products. Complementary analytical approaches are thus often used for comprehensive screening of herbs and spices. In this study we evaluate DNA metabarcoding for the identification and authentication of 62 products, containing basil, oregano, and paprika collected from different retailers and importers in Norway. Our results show varying degrees of discrepancy between the constituent species and those listed on the product labels, despite high product authenticity. We suggest the false positives result from the sensitivity of DNA metabarcoding and filtering thresholds should be integrated into protocols to reduce false positives. Our results highlight how integrating DNA metabarcoding into the toolbox of analytical methods for quality control of fresh and/or processed plant-based food can improve product quality.

scholarly journals A Review of the Botany, Traditional Use, Phytochemistry, Analytical Methods, Pharmacological Effects, and Toxicity of Angelicae Pubescentis Radix

2020 ◽  
Vol 2020 ◽  
pp. 1-28
Author(s):  
Liu Yang ◽  
Ajiao Hou ◽  
Song Wang ◽  
Jiaxu Zhang ◽  
Wenjing Man ◽  
...  
Keyword(s):  
Quality Control ◽  
Mechanism Of Action ◽  
Analytical Methods ◽  
Lumbar Disc ◽  
Pharmacological Study ◽  
Relevant Information ◽  
Chemical Components ◽  
Pharmacological Effects ◽  
Bioactive Constituents ◽  
Traditional Use

Angelicae Pubescentis Radix (AP), as a traditional Chinese medicine (TCM), has been used for thousands of years in China. In this paper, the botany, traditional use, phytochemistry, analytical methods, quality control, pharmacological effects, and toxicity of AP were reviewed. It can provide a reference for the further research and lay a foundation for the rational clinical application of AP. The relevant information on AP was collected from scientific databases (such as Baidu Scholar, CNKI, Google Scholar, PubMed, Science Direct, Web of Science, and SciFinder Scholar), Chinese herbal classics, Chinese Pharmacopoeia, PhD and MSc dissertations, and so on. The components which have been isolated and identified in AP include coumarins, volatile oils, organic acids, terpenes, polysaccharides, flavonoids, sterols, and trace elements. Most of them were analyzed by HPLC and GC. A pharmacological study shows that the AP has extensive pharmacological effects, including anti-inflammatory, antirheumatism, sedative and hypnotic, neuroprotection, antioxidation, antitumor, and allergy, and it is widely used in the treatment of the rheumatoid arthritis, knee osteoarthritis, lumbar disc, ankylosing spondylitis, headaches, stroke hemiplegia, Alzheimer’s, and arrhythmia. AP is a valuable natural medicinal plant. So far, significant advances have been made in phytochemistry and pharmacology. Some traditional uses have been demonstrated by modern pharmacology. However, the chemical components and pharmacological effects of AP are complex and varied, and there are different standards for the evaluation of its quality and efficacy. The mechanism of action, the structure-activity relationship among the components, and the potential synergistic and antagonistic effects remain to be studied. At the same time, there are few studies on the specific compounds related to its pharmacodynamics. In order to better develop and utilize AP, we should establish a more reasonable, reliable, and accurate quality control standard and focus on the study of bioactive constituents and the demonstration of their mechanism of action.

DIMENSIONING OF ORTHOTROPICALLY STIFFENED CFRP SHELLS OF LARGE LAUNCH VEHICLES FOR LOAD INTRODUCTION AND STABILITY

2010 ◽  
Vol 10 (04) ◽  
pp. 601-621 ◽  
Author(s):  
ANDREAS RITTWEGER ◽  
SUSANNE CHRISTIANSON ◽  
HUBA ÖRY
Keyword(s):  
Analytical Methods ◽  
Flux Distribution ◽  
Fe Analysis ◽  
Launch Vehicles ◽  
Local Instability ◽  
Axial Loads ◽  
Ring Frame ◽  
Final Design ◽  
Shell Equations ◽  
Analytical Approaches

The dimensioning of an orthotropically stiffened cylindrical CFRP shell subjected to the introduction of concentrated axial loads using rapid analytical methods is presented. For stress calculation the shell equations are simplified by applying the semibending theory and integrated by employing the transfer matrix method. Analytical approaches are used for stability verification. The dimensioning considers required constraints in the force flux distribution, strength of the laminate, general instability, panel instability (from ring frame to ring frame) and local instability. The rapid analytical methods allow mass optimization. The final design is confirmed by detailed FE analysis. A comparison of the FE analysis with the analytical results is shown.

Comparison of analytical methods for the quality control of a new formulation containing soy extract and melatonin

2008 ◽  
Vol 31 (10) ◽  
pp. 1851-1859 ◽  
Author(s):  
Maria Addolorata Saracino ◽  
Laura Mercolini ◽  
Alessandro Musenga ◽  
Francesca Bugamelli ◽  
Maria Augusta Raggi
Keyword(s):  
Quality Control ◽  
Analytical Methods ◽  
New Formulation ◽  
Comparison Of Analytical Methods

scholarly journals Goji Berry: Quality Assessment and Crop Adaptation of Plants Cultivated in Tuscany (Italy) by Combination of Carotenoid and DNA Analyses

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Giada Capecchi ◽  
Emanuele Goti ◽  
Elena Nicolai ◽  
Maria Camilla Bergonzi ◽  
Roberto Monnanni ◽  
...  
Keyword(s):  
Quality Control ◽  
Dna Barcoding ◽  
Analytical Methods ◽  
Hplc Analysis ◽  
European Pharmacopoeia ◽  
Plant Adaptation ◽  
Dna Analyses ◽  
Berry Quality ◽  
Quality Markers ◽  
Crucial Information

In this study HPLC analysis for the evaluation of carotenoids and DNA barcoding are reported for three different samples of Lycium cultivated in Tuscany (Italy). These two analytical methods can represent integrative methods for quality control of goji, giving also crucial information on the plant adaptation to different environments. Hence, carotenoids represent the quality markers proposed by the monograph of the European Pharmacopoeia, while DNA barcoding can differentiate between species and populations and is useful for the detection of the homogeneity of the samples.

scholarly journals Tetraconatan phylogeny with special focus on Malacostraca and Branchiopoda: highlighting the strength of taxon-specific matrices in phylogenomics

2018 ◽  
Vol 285 (1885) ◽  
pp. 20181524 ◽  
Author(s):  
Martin Schwentner ◽  
Stefan Richter ◽  
D. Christopher Rogers ◽  
Gonzalo Giribet
Keyword(s):  
Amino Acid ◽  
Analytical Methods ◽  
Special Focus ◽  
Evolutionary Models ◽  
Phylogenomic Analyses ◽  
New Generation ◽  
Analytical Approaches ◽  
Internal Relationships ◽  
Complete Matrix ◽  
Phylogenomic Study

Understanding the evolution of Tetraconata or Pancrustacea—the clade that includes crustaceans and insects—requires a well-resolved hypothesis regarding the relationships within and among its constituent taxa. Here, we assembled a taxon-rich phylogenomic dataset focusing on crustacean lineages based solely on genomes and new-generation Illumina-generated transcriptomes, including 89 representatives of Tetraconata. This constitutes, to our knowledge, the first phylogenomic study specifically addressing internal relationships of Malacostraca (with 26 species included) and Branchiopoda (36 species). Seven matrices comprising 81–684 orthogroups and 17 690–242 530 amino acid positions were assembled and analysed under five different analytical approaches. To maximize gene occupancy and to improve resolution, taxon-specific matrices were designed for Malacostraca and Branchiopoda. Key tetraconatan taxa (i.e. Oligostraca, Multicrustacea, Branchiopoda, Malacostraca, Thecostraca, Copepoda and Hexapoda) were monophyletic and well supported. Within Branchiopoda, Phyllopoda, Diplostraca, Cladoceromorpha and Cladocera were monophyletic. Within Malacostraca, the clades Eumalacostraca, Decapoda and Reptantia were well supported. Recovery of Caridoida or Peracarida was highly dependent on the analysis for the complete matrix, but it was consistently monophyletic in the malacostracan-specific matrices. From such examples, we demonstrate that taxon-specific matrices and particular evolutionary models and analytical methods, namely CAT-GTR and Dayhoff recoding, outperform other approaches in resolving certain recalcitrant nodes in phylogenomic analyses.

Statistical Evaluation of the Results Obtained with the Analytical Methods used for the Quality Control of Medicines

1987 ◽  
Vol 13 (14) ◽  
pp. 2571-2615 ◽  
Author(s):  
Luigi Cavenaghi ◽  
Gian Gualberto Gallo ◽  
Gian Marco Leali ◽  
Silvano Vianello
Keyword(s):  
Quality Control ◽  
Analytical Methods ◽  
Statistical Evaluation
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