scholarly journals Identification and Validation of Stable Quantitative Trait Loci for SDS-Sedimentation Volume in Common Wheat (Triticum aestivum L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Shuai Tian ◽  
Minghu Zhang ◽  
Jinghui Li ◽  
Shaozhe Wen ◽  
Chan Bi ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Common Wheat ◽  
Quantitative Trait ◽  
Positional Cloning ◽  
Sodium Dodecyl ◽  
Snp Markers ◽  
Reference Sequence ◽  
Phenotypic Variance ◽  
Dodecyl Sulfate ◽  
Sedimentation Volume

Sodium dodecyl sulfate-sedimentation volume is an important index to evaluate the gluten strength of common wheat and is closely related to baking quality. In this study, a total of 15 quantitative trait locus (QTL) for sodium dodecyl sulfate (SDS)-sedimentation volume (SSV) were identified by using a high-density genetic map including 2,474 single-nucleotide polymorphism (SNP) markers, which was constructed with a doubled haploid (DH) population derived from the cross between Non-gda3753 (ND3753) and Liangxing99 (LX99). Importantly, four environmentally stable QTLs were detected on chromosomes 1A, 2D, and 5D, respectively. Among them, the one with the largest effect was identified on chromosome 1A (designated as QSsv.cau-1A.1) explaining up to 39.67% of the phenotypic variance. Subsequently, QSsv.cau-1A.1 was dissected into two QTLs named as QSsv.cau-1A.1.1 and QSsv.cau-1A.1.2 by saturating the genetic linkage map of the chromosome 1A. Interestedly, favorable alleles of these two loci were from different parents. Due to the favorable allele of QSsv.cau-1A.1.1 was from the high-value parents ND3753 and revealed higher genetic effect, which explained 25.07% of the phenotypic variation, mapping of this locus was conducted by using BC3F1 and BC3F2 populations. By comparing the CS reference sequence, the physical interval of QSsv.cau-1A.1.1 was delimited into 14.9 Mb, with 89 putative high-confidence annotated genes. SSVs of different recombinants between QSsv.cau-1A.1.1 and QSsv.cau-1A.1 detected from DH and BC3F2 populations showed that these two loci had an obvious additive effect, of which the combination of two favorable loci had the high SSV, whereas recombinants with unfavorable loci had the lowest. These results provide further insight into the genetic basis of SSV and QSsv.cau-1A.1.1 will be an ideal target for positional cloning and wheat breeding programs.

D-genome suppression of gliadin electrophoretic band 45 in hexaploid wheat and the effect upon quality

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 395-399 ◽  
Author(s):  
N. K. Howes ◽  
M. I. Kovacs ◽  
E. R. Kerber
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Hexaploid Wheat ◽  
Protein Gene ◽  
Sodium Dodecyl ◽  
D Genome ◽  
Electrophoretic Band ◽  
Endosperm Protein ◽  
Dodecyl Sulfate ◽  
Sedimentation Volume ◽  
The Cross

The hexaploid wheat 'Prelude' (2n = 42 = AABBDD) contains the endosperm protein electrophoretic band 40, but not band 45, while the extracted tetraploid 'Tetraprelude' (2n = 28 = AABB) contains electrophoretic band 45 but not band 40. Moreover, F3 progeny from the cross between backcross lines of 'Prelude' and 'Marquis' segregated as if both of these proteins were controlled by alleles of one gene, or by two very closely linked genes. Examination of the F3 seed from the cross 'Prelude' × 'Canthatch' 7D nullisomic showed that band 45 was not expressed in any of the 334 F2 families, including nine nullisomics. Together, these results indicate that band 45 endosperm protein is not suppressed by a gene on chromosome 7D. Furthermore, removal of the D genome from 'Prelude' resulted in increased kernel hardness and increased gluten elastic strength but a decrease in sodium dodecyl sulfate sedimentation volume. However, the sodium dodecyl sulfate sedimentation volume of 'Tetraprelude', was the highest among the tetraploid cultivars evaluated.Key words: wheat, sodium dodecyl sulfate electrophoresis, endosperm protein, gene expression.

scholarly journals Effect of Glu-B3 Allelic Variation on Sodium Dodecyl Sulfate Sedimentation Volume in Common Wheat (Triticum aestivumL.)

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Hongqi Si ◽  
Manli Zhao ◽  
Fuxia He ◽  
Chuanxi Ma
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Allelic Variation ◽  
Sodium Dodecyl ◽  
Bread Making ◽  
Specific Pcr ◽  
Dodecyl Sulfate ◽  
Allele Specific ◽  
Sedimentation Volume ◽  
Improved Cultivars ◽  
Allele Specific Pcr

Sodium dodecyl sulfate (SDS) sedimentation volume has long been used to characterize wheat flours and meals with the aim of predicting processing and end-product qualities. In order to survey the influence of low-molecular-weight glutenin subunits (LMW-GSs) at Glu-B3 locus on wheat SDS sedimentation volume, a total of 283 wheat (Triticum aestivumL.) varieties including landraces and improved and introduced cultivars were analyzed using 10 allele-specific PCR markers at the Glu-B3 locus. The highest allele frequency observed in the tested varieties was Glu-B3i with 21.9% in all varieties, 21.1% in landraces, 25.5% in improved cultivars, and 12% in introduced cultivars. Glu-B3 locus represented 8.6% of the variance in wheat SDS sedimentation volume, and Glu-B3b, Glu-B3g, and Glu-B3h significantly heightened the SDS sedimentation volume, but Glu-B3a, Glu-B3c, and Glu-B3j significantly lowered the SDS sedimentation volume. For the bread-making quality, the most desirable alleles Glu-B3b and Glu-B3g become more and more popular and the least desirable alleles Glu-B3a and Glu-B3c got less and less in modern improved cultivars, suggesting that wheat grain quality in China has been significantly improved through breeding effort.

Solubility and adsorption behaviors of chlorophenols in the presence of surfactant

1997 ◽  
Vol 35 (7) ◽  
pp. 123-130 ◽  
Author(s):  
J. C. Liu ◽  
P. S. Chang
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Critical Micelle Concentration ◽  
Sodium Dodecyl ◽  
Adsorption Behaviors ◽  
Brij 35 ◽  
Important Index ◽  
Dodecyl Sulfate ◽  
Triton X

The solubility of chlorophenols as affected by surfactant was investigated. Three kinds of surfactant, sodium dodecyl sulfate, Triton X-100, and Brij 35, were utilized. The solubilization of chlorophenols by surfactant follows the order of 2,4,6-trichlorophenol > 2,4-dichlorophenol > 2,6-dichlorophenol > 2-chlorophenol; and the critical micelle concentration is an important index. The adsorption reactions of 2,4-dichlorophenol and 2,4,6- trichlorophenol onto hydrous montmorillonite in the presence of surfactant were examined. The presence of surfactant decreased the adsorption of chlorophenols significantly. The roles of hydrophobicity of chlorophenols in solubilization and adsorption behaviors are discussed.

Different submicellar solubilization mechanisms revealed by 1H NMR and 2D diffusion ordered spectroscopy (DOSY)

2020 ◽  
Vol 22 (19) ◽  
pp. 11075-11085
Author(s):  
Mengjian Wu ◽  
Zhaoxia Wu ◽  
Shangwu Ding ◽  
Zhong Chen ◽  
Xiaohong Cui
Keyword(s):  
Nmr Spectroscopy ◽  
Sodium Dodecyl Sulfate ◽  
1H Nmr ◽  
Sodium Dodecyl ◽  
Butyl Methacrylate ◽  
H Nmr ◽  
Molecular Scale ◽  
Two Systems ◽  
Dodecyl Sulfate ◽  
Triton X

Different submicellar solubilization mechanisms of two systems, Triton X-100/tetradecane and sodium dodecyl sulfate (SDS)/butyl methacrylate, are revealed on the molecular scale by 1H NMR spectroscopy and 2D diffusion ordered spectroscopy (DOSY).

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