scholarly journals Reference Ranges for Hematological and Biochemical Profile of Martina Franca Donkeys

2020 ◽  
Vol 7 ◽  
Author(s):  
Francesca Trimboli ◽  
Ippolito De Amicis ◽  
Antonio Di Loria ◽  
Carlotta Ceniti ◽  
Augusto Carluccio
Keyword(s):  
Blood Cell ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Conservation Strategies ◽  
Total Serum ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Platelet Volume ◽  
Distribution Width ◽  
Glutamyl Transferase

The Martina Franca donkey (MFd) is one of the largest Italian donkey breeds, considered as endangered breed. To support the conservation strategies, knowledge about the physiologic hematological parameters of MFds is needed. The aims of the study were to determine reference value for hematological and major serum parameters in a population of healthy MFds and to estimate the influence of age on these parameters. Eighty-one clinically healthy MFds (17 males and 64 females) in different ages were enrolled: group A (foals, n° 16, animals < 1 year old) group B (young animals, n° 36, from 1 to 3 years old), and group C (adult animals, n° 29, over 3 years old). Red blood cell count (RBC); hematocrit value (HCT); hemoglobin concentration (HGB); mean corpuscular volume (MCV); mean corpuscular hemoglobin (MCH); hemoglobin concentration distribution width (HDW); RBC distribution width (RDW); total white blood cell (WBC); WBC differential count for neutrophils, lymphocytes, monocytes, eosinophils and basophils, and platelets (PLT); mean platelet volume (MPV); platelet volume distribution width; and plateletcrit (PCT) were analyzed. The biochemistry panel included aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), total serum protein (TP), albumin (ALB), cholesterol (CHOL), triglyceride (TGL), blood urea nitrogen (BUN), creatinine (CREA), glucose (GLU), Ca2+, phosphorus, Na+, Cl−, K+, and Mg2+. The effect of age on hematological parameters was investigated using one-way ANOVA test. Age of donkeys does not influence total WBC, HGB, HCT, platelet count and MPV, and PCT (P > 0.05). Some leukocyte populations such as eosinophils, monocytes, and basophils showed age-linked variations (P < 0.05). RBC count, RDW, and HDW decrease with age whereas MCV and MCH increase. Na+, K+, Cl−, Ca2+, phosphorus, ALP, GGT, CREA, GLUC, and CHOL decrease with age (P < 0.05), while AST and TP showed an increase with aging (P < 0.05). ALB reaches the lowest values in young donkeys and returns to values of foals in older animals (P < 0.05). Finally, a difference among groups for BUN and TGL was not found (P < 0.05). The results suggest how even for the MFd breed, age is a variable that affects different hematological and biochemical parameters. Compared to other donkey and horses, the MFd breed showed some differences that clinicians involved during conservation strategies need to be consider.

scholarly journals Hematology of Wild Caught Dubois’s Tree FrogPolypedates teraiensis, Dubois, 1986 (Anura: Rhacophoridae)

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Madhusmita Das ◽  
Pravati Kumari Mahapatra
Keyword(s):  
Blood Cell ◽  
Blood Serum ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Corpuscular Hemoglobin ◽  
Differential Leukocyte Count ◽  
Rbc Count

Blood was analyzed from eighty (forty males and forty females) adult individuals ofPolypedates teraiensisto establish reference ranges for its hematological and serum biochemical parameters. The peripheral blood cells were differentiated as erythrocytes, lymphocytes, eosinophils, neutrophils, monocytes, basophils, and thrombocytes, with similar morphology to other anurans. Morphology of blood cells did not vary according to sex. The hematological investigations included morphology and morphometry of erythrocytes, morphometry of leucocytes, packed cell volume (PCV), hemoglobin content (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), erythrocyte or red blood cell (RBC) count, leukocyte or white blood cell (WBC) count, differential leukocyte count, and neutrophil to lymphocyte (N/L) ratio. Besides, protein, cholesterol, glucose, urea, uric acid, and creatinine content of blood serum were assayed. Hematological parameters that differed significantly between sexes were RBC count, length and breadth of RBC, neutrophil %, N/L ratio, area occupied by basophils, and diameter of large lymphocyte and eosinophils. The level of glucose, urea, and creatinine in blood serum also significantly differed between sexes.

scholarly journals Variation of Red Blood Cell Distribution Width and Mean Platelet Volume after Moderate Endurance Exercise

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Giuseppe Lippi ◽  
Gian Luca Salvagno ◽  
Elisa Danese ◽  
Cantor Tarperi ◽  
Gian Cesare Guidi ◽  
...  
Keyword(s):  
Blood Cell ◽  
Endurance Exercise ◽  
Red Blood Cell ◽  
Mean Platelet Volume ◽  
Complete Blood Count ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Platelet Volume ◽  
Distribution Width ◽  
Haemoglobin Content

Although physical exercise strongly influences several laboratory parameters, data about the hematological changes after medium distance running are scarce. We studied 31 middle-trained athletes (mean training regimen217±32 min/week) who performed a 21.1 km, half-marathon run. Blood samples were collected before the run, at the end, and 3 and 20 hours thereafter. The complete blood count was performed on Advia 2120 and included red blood cell (RBC), reticulocyte, and platelet counts; hemoglobin; mean corpuscular volume (MCV); mean corpuscular hemoglobin (MCH); reticulocyte haemoglobin content (Ret CHR); RBC distribution width (RDW), mean platelet volume (MPV). No significant variations were observed for MCH and Ret CHR. The RBC, reticulocyte, and hemoglobin values modestly decreased after the run. The MCV significantly increased at the end of running but returned to baseline 3 hours thereafter. The RDW constantly increased, reaching a peak 20 hours after the run. The platelet count and MPV both increased after the run and returned to baseline 3 hours thereafter. These results may have implications for definition of reference ranges and antidoping testing, and may also contribute to explaining the relationship between endurance exercise and mortality, since previous studies reported that RDW and MPV may be significantly associated with cardiovascular disease.

scholarly journals Red blood cell profile in patients with mild, moderate and severe COVID-19

2021 ◽  
Vol 15 (2) ◽  
pp. 26-31
Author(s):  
Khushbhun Nahar Layla ◽  
Shahanara Yeasmin ◽  
Afrina Binte Azad ◽  
Masba Uddin Chowdhury ◽  
Nasrin Sultana ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Significant Negative Correlation ◽  
Mean Corpuscular Hemoglobin ◽  
Rt Pcr ◽  
Distribution Width ◽  
Significant Difference ◽  
Bonferroni Test

Background and objectives: Coronavirus disease 2019 (COVID-19) pandemic has affected millions of people world-wide. It is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Increasing evidence has shown abnormalities of different hematological parameters with the severity of the diseases. The present study was undertaken to determine the red blood cell (RBC) profile in different categories of COVID-19 patients. Materials and methods: The study was conducted from January 2020 to December 2020. Reverse transcriptase-polymerase chain reaction (RT-PCR) positive COVID-19 patients were enrolled. Patients were categorized into mild, moderate and severe COVID-19 cases. Blood samples were analyzed by Automated Hematology Analyzer for hemoglobin concentration, total erythrocyte count and RBC indices. ANOVA followed by Bonferroni test, Chi square test, Spearman’s rho correlation coefficient test were performed as applicable using SPSS version 25.0. Results: A total of 100 RT-PCR positive COVID-19 patients were included in the study. There were 25, 38 and 37 mild, moderate and severe cases respectively. The mean age of the study participants was 44.68 + 13.16 years (range: 18 to 65 years). There were 67 (67%) males and 33 (33%) females. No significant difference in hemoglobin (Hb), hematocrit (HCT), total RBC count, red blood cell distribution width (CDW) was observed among the three groups. Significant negative correlation of mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH); rs-0.362 & -0.255 respectively) was observed with disease severity. Conclusion: The study showed low MCV and MCH were significantly related with the severity of the COVID-19 illness. Therefore, comprehensive analysis of the RBC profile would be helpful to understand the disease course. Ibrahim Med. Coll. J. 2021; 15(2): 26-31

scholarly journals Impacts of Mycoplasma loads and lung lesions on immune and hematological statuses of pigs in an eight-breed cross heterogeneous population

2020 ◽  
Vol 98 (8) ◽  
Author(s):  
Qing Zhang ◽  
Jing Li ◽  
Tao Huang ◽  
Yifeng Zhang ◽  
Wenwu Xu ◽  
...  
Keyword(s):  
Blood Cell ◽  
Neutrophil Count ◽  
Lymphocyte Count ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Volume Distribution ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Distribution Width ◽  
Lung Lesions

Abstract Developments of pulmonary diseases, often accompanied by infections of bacteria, severely affect the meat production and welfare of pigs. This study investigated 307 pigs at age of 240 d from an eight-breed cross reared under standardized housing conditions for associations among the extent of lung lesions, bacteria load inferred from 16S rRNA sequencing of bronchoalveolar lavage fluid, as well as 57 immune cells and 25 hematological traits. We showed that the pigs under study suffered substantial and varied lung lesions, and the Mycoplasma is the most associated bacteria genera. At a false discovery rate of 0.05 (FDR < 0.05), the severity of lung lesions were significantly associated with greater CD8+ to CD3+ cell ratio, neutrophil-to-lymphocyte ratio (NLR), and standard deviation of red blood cell volume distribution width (RDW-SD), and lower CD4−CD8−/CD3+, CD3+CD4−CD8−/PBMCs (peripheral blood mononuclear cells) and CD14−CD16−/PBMCs cell ratios, mean corpuscular hemoglobin concentration, lymphocyte count, and lymphocyte count percentage, reflecting an status of inflammation, immune suppression, and hypoxia of the pigs accompanying the progression of the lung lesions. The Mycoplasma abundance showed positive correlations with neutrophil count, neutrophil count percentage, NLR, monocyte count, coefficient of variation in red blood cell volume distribution width , and RDW-SD, and negative correlations with mean corpuscular hemoglobin concentration, lymphocyte count, and lymphocyte count percentage; these correlations are largely consistent with those of lung lesions, supporting the comorbidity of lung lesions and Mycoplasma infection. We also observed nonlinear associations that sharp increases in neutrophil count and neutrophil count percentage occurred only when Mycoplasma abundance raised above the population-average level. The results provide helpful insights into the changes of host immune status in response to Mycoplasma relevant lung diseases in pigs.

scholarly journals Hematological parameters in Nile Tilápia, Oreochromis niloticus exposed to sub-letal concentrations of mercury

2007 ◽  
Vol 50 (4) ◽  
pp. 619-626 ◽  
Author(s):  
Nilton Massuo Ishikawa ◽  
Maria José Tavares Ranzani-Paiva ◽  
Julio Vicente Lombardi ◽  
Cláudia Maris Ferreira
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Oreochromis Niloticus ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Set Up

Mercury toxicity in tilapia, Oreochromis niloticus, (Linnaeus, 1758) was investigated by the hematological parameters after long-term (14 days) exposure to various Hg concentrations (0.02, 0.002, 0.0002mg/L Hg). Test groups were set up with three replicates for each concentration, plus the control group. Blood samples were collected from six individuals for each concentration at 0, 3, 7, 10 and 14 days of exposure. The hematological parameters analyzed were: total red blood cell count (RBC), hemoglobin concentration (Hb), hematocrit (Ht), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), total white blood cell count (WBC) and differential leukocyte counts and total thrombocyte count (Tr). There were no significant differences among the mean hematological values at the different Hg concentrations indicating that Hg at the concentrations studied was not toxic to tilapia.

scholarly journals Red Blood Cell Variables in Children and Adolescents regarding the Age and Sex

2019 ◽  
Author(s):  
Jasmina PLUNCEVIC GLIGOROSKA ◽  
Serjoza GONTAREV ◽  
Beti DEJANOVA ◽  
Lidija TODOROVSKA ◽  
Daniela SHUKOVA STOJMANOVA ◽  
...  
Keyword(s):  
Blood Cell ◽  
Children And Adolescents ◽  
Red Blood Cell ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Female Group ◽  
Distribution Width ◽  
Hematological Indices ◽  
Significant Difference ◽  
Age And Sex

Background: This study aimed to assess the basic red blood cell variables and hematological indices in children and adolescents and analyze the differences regarding age and sex. Methods: Overall, 320 young participants, age 8 to 18 yr, were enrolled at Laboratory of Sport’s Medicine, Medical Faculty, Skopje, Macedonia in 2016. Capillary blood samples were drawn and following hematologic parameters were measured: the red blood cell count (RBC), hemoglobin concentration (Hb), hematocrit level (Hct) and hematological indexes: mean corpuscular volume (MCV), mean hemoglobin concentration (MCH), mean corpuscular hemoglobin concentration (MCHC) and red cell distribution width (RDW). Results: RBC variables in male group showed high statistical level of significance between age different groups (P=0.001) for all studied parameters except MCHC (P=0.423) and RDW (P=0.174). ANOVA test and multivariate tests in female group showed that there was no significant difference for all hematological parameters between age different groups. Regarding the sex differences, male participants had significantly higher red blood count (P<0.001), hemoglobin content (P<0.001) and hematocrit (P<0.001). Conclusion: Hematological parameters in adolescent as inhomogeneous population are not quantified sufficiently, especially hematological indices. RBC variables, regardless of the age, differ very much between male and female examinees, in favor of the male examinees. Hematological indices were insignificantly higher in males. Regarding the age of examinees, RBC variables showed significant inter-groups differences only within male adolescents. While with girls, ages span 8 to 18 yr, we did not find significant differences for most of the hematological variables.

scholarly journals Complete Blood Count Peculiarities in Pregnant SARS-CoV-2-Infected Patients at Term: A Cohort Study

Diagnostics ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 80
Author(s):  
Roxana Covali ◽  
Demetra Socolov ◽  
Razvan Socolov ◽  
Ioana Pavaleanu ◽  
Alexandru Carauleanu ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Count ◽  
Complete Blood Count ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Distribution Width

Background: During viral outbreaks, pregnancy poses an increased risk of infection for women. Methods: In a prospective study, all patients admitted for delivery at term to Elena Doamna Obstetrics and Gynecology University Hospital in Iasi, Romania, between 1 April 2020 and 31 December 2020 were included. There were 457 patients, divided into two groups: group 1, SARS-CoV-2-positive patients (n = 46) and group 2, SARS-CoV-2-negative patients (n = 411). Among other tests, complete blood count was determined upon admittance, and the following values were studied: white blood cell count, lymphocytes, neutrophils, red blood cell count, hemoglobin, mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, mean corpuscular volume, red blood cell distribution width, hematocrit, platelet count, mean platelet volume, platelet distribution width, plateletcrit, and platelet large cell ratio. Results: in pregnant SARS-CoV-2-infected patients at term, there was a significant decrease in white blood cell, neutrophil, and lymphocyte count, and an increase in mean corpuscular hemoglobin concentration, compared to healthy pregnant women at term, although all still within normal limits. None of the other components of the complete blood count or fetal outcomes studied was significantly influenced by SARS-CoV-2 infection in pregnant patients at term.

Impact of weight loss on inflammation and red blood cell biomarkers after laparoscopic gastric banding surgery

2017 ◽  
Vol 66 (2) ◽  
pp. 304-308 ◽  
Author(s):  
Susana Coimbra ◽  
Cátia Ferreira ◽  
Luís Belo ◽  
Petronila Rocha-Pereira ◽  
Alice Catarino ◽  
...  
Keyword(s):  
Weight Loss ◽  
Blood Cell ◽  
Gastric Banding ◽  
Iron Metabolism ◽  
Red Blood Cell ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Obese Patients ◽  
Corpuscular Hemoglobin ◽  
Distribution Width

Adipose tissue produces several adipokines that are enrolled in different metabolic and inflammatory pathways that may disturb iron metabolism and erythropoiesis. Considering that laparoscopic adjustable gastric banding (LAGB) has not been associated with a long-term risk of malabsorption, we performed a 13-month follow-up study in severe obese patients submitted to LAGB in order to clarify its impact on inflammation, iron metabolism and on red blood cell (RBC) biomarkers. Twenty obese patients were enrolled in the study, being clinical and analytically assessed before (T0) and 13 months after LAGB intervention (T1). Inflammation, iron bioavailability and RBC biomarkers were evaluated at T0 and T1. At T1, weight and anthropometric indices decreased significantly; patients showed a significant increase in mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration (MCHC), and a reduction in red cell distribution width, ferritin, hepcidin, tumor necrosis factor-α, interleukin-6 (IL-6) and C-reactive protein. Before LAGB, IL-6 correlated negatively with iron, hemoglobin concentration and MCHC; hepcidin correlated inversely with transferrin. Our data show that 13 months after LAGB, the weight loss is associated with an improvement in inflammation, namely a reduction in IL-6 that may reduce hepcidin production, improving iron availability for erythropoiesis, as shown by more adequate erythrocyte hemoglobinization.

scholarly journals Hematology Safety Data for the Fermented Soy Beverage Q-CAN® Plus (P12-033-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Wajahat Mehal ◽  
Arumugam Suyavaran ◽  
Evangelos Dioletis ◽  
Ricardo Paiva ◽  
Eric Secor ◽  
...  
Keyword(s):  
Hematological Parameters ◽  
Safety Data ◽  
Hemoglobin Concentration ◽  
Post Treatment ◽  
Biological Research ◽  
Mean Corpuscular Hemoglobin ◽  
New Haven ◽  
Corpuscular Hemoglobin ◽  
Distribution Width ◽  
Blood Indices

Abstract Objectives In this study we want to investigate the effects of Q-CAN® Plus, the nutritional fermented soy drink on the hematological parameters in lean and obese individuals. Methods Prospective study comprised of lean (10) and obese (10) individuals without a known diagnosis. 11 clinic visits over 14 weeks (visit 1/wk −2, visit 2/wk −1, visit 3/wk 0, visit 4/wk 1, visit 5/wk 2, visit 6/wk 3, visit 7/wk 4, visit 8/wk 6, visit 9/wk 8, visit 10/wk 10, visit 11/wk 12). Visits 1, 2 and 3 occurred prior to treatment. 237 ml of soy was dispensed at visit 3 and was consumed twice daily for 4 weeks until visit 7 (weeks 1–4). Visits 8, 9, 10 and 11 were post treatment. Blood was collected during visits 1, 3, 5, 7, 8, 9, 10,11, and various hematological parameters were quantified at Yale New haven health, hematology lab at the Department of laboratory medicine (Yale new haven health, Yale University, CT, USA). The values were statistically analyzed using one-way ANOVA and the results were considered significant for P < 0.05. Results Various blood indices such as mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), mean platelet volume (MPV), red blood cell distribution width (RDW-CV), hematocrit, RBC count, WBC count, % neutrophils, average number of neutrophils (# neutrophils), % monocytes, % basophils, average number of lymphocytes (# lymphocytes), % eosinophils and C- reactive protein were analyzed. These values were compared as plot curve for values from individual visits of each subject, as averages of combined values of both lean and obese individuals grouped as (pre, during and post) treatment periods of BESO soy drink, as averages of lean vs obese grouped for various treatment periods of BESO soy drink and as ratio of averages of lean vs obese group values for various treatment periods of BESO soy drink. The consumption of Q-CAN® Plus did not result in significant changes in any hematological parameters Conclusions Q-CAN® Plus fermented soy drink does not induce any significant change in the hematological parameters of both lean and obese individuals. These observations demonstrate the safety of Q-CAN® Plus regarding hematological parameters in lean and obese individuals. Funding Sources Beso Biological Research, Inc.

scholarly journals The Effect of One Bout of Intensive Judo Exercise on Select Hematological and Immunological Parameters in Adolescent Elite Judo Athletes

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Mohammad-Reza Hazratian ◽  
Hossein Taheri-Chadorneshin ◽  
Asmat Rashidi
Keyword(s):  
Blood Cell ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Adolescent Male ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Immunological Parameters ◽  
Distribution Width ◽  
Humoral Immune ◽  
Paired Samples

Background: Exercise-related studies do not agree on changes in blood parameters regarding anemia and serum immunoglobulin concentration as adaptive immunity. Objectives: The current study aimed to investigate exercise-induced variations in hematological and immunological parameters in response to one bout of intensive judo exercise in adolescent elite judoka. Methods: Venipuncture was performed on ten adolescent male Iranian elite judoka (age: 15.60 ± 0.69 years; body mass index: 24.15 ± 2.80 kg/m2) before and immediately after one bout of intensive judo protocol. Erythrocyte variables [red blood cell count (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), red distribution width (RDW), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC)], platelets variables [platelet count (PLT), platelet distribution width (PDW), mean platelet volume (MPV), plateletcrit (PCT)], cellular immune variables [white blood cell (WBC), neutrophil (NEU), lymphocyte (LYM)], and humoral immune variables (IgM, IgA, IgG, IgE) were measured. Data were analyzed using Wilcoxon and paired-samples t-test, and P < 0.05 was considered significant. Results: After the plasma volume was corrected using the Dill and Costill equation, the results showed that RBC, HGB, HCT, MCHC, IgA, and IgM decreased significantly immediately after exercise. However, MCH, RDW, PLT, PDW, MPV, PCT, WBC, LYM, NEU, IgG, and IgE did not change significantly. Conclusions: According to the findings, it can be concluded that intensive judo exercise results in transient anemia and suppression of humoral immune response among adolescent elite judoka.

Sign in / Sign up

Export Citation Format

Share Document