scholarly journals The Effect of One Bout of Intensive Judo Exercise on Select Hematological and Immunological Parameters in Adolescent Elite Judo Athletes

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Mohammad-Reza Hazratian ◽  
Hossein Taheri-Chadorneshin ◽  
Asmat Rashidi
Keyword(s):  
Blood Cell ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Adolescent Male ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Immunological Parameters ◽  
Distribution Width ◽  
Humoral Immune ◽  
Paired Samples

Background: Exercise-related studies do not agree on changes in blood parameters regarding anemia and serum immunoglobulin concentration as adaptive immunity. Objectives: The current study aimed to investigate exercise-induced variations in hematological and immunological parameters in response to one bout of intensive judo exercise in adolescent elite judoka. Methods: Venipuncture was performed on ten adolescent male Iranian elite judoka (age: 15.60 ± 0.69 years; body mass index: 24.15 ± 2.80 kg/m2) before and immediately after one bout of intensive judo protocol. Erythrocyte variables [red blood cell count (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), red distribution width (RDW), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC)], platelets variables [platelet count (PLT), platelet distribution width (PDW), mean platelet volume (MPV), plateletcrit (PCT)], cellular immune variables [white blood cell (WBC), neutrophil (NEU), lymphocyte (LYM)], and humoral immune variables (IgM, IgA, IgG, IgE) were measured. Data were analyzed using Wilcoxon and paired-samples t-test, and P < 0.05 was considered significant. Results: After the plasma volume was corrected using the Dill and Costill equation, the results showed that RBC, HGB, HCT, MCHC, IgA, and IgM decreased significantly immediately after exercise. However, MCH, RDW, PLT, PDW, MPV, PCT, WBC, LYM, NEU, IgG, and IgE did not change significantly. Conclusions: According to the findings, it can be concluded that intensive judo exercise results in transient anemia and suppression of humoral immune response among adolescent elite judoka.

scholarly journals A New Approach to Blood Parameters in Dogs with Hemorrhagic Enteritis

2017 ◽  
Vol 45 (1) ◽  
pp. 6
Author(s):  
Handan Hilal Arslan ◽  
Murat Guzel ◽  
Yucel Meral ◽  
Duygu Dalgin ◽  
Guvenc Gokalp ◽  
...  
Keyword(s):  
Blood Cell ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Mean Corpuscular Hemoglobin ◽  
Red Cell ◽  
Corpuscular Hemoglobin ◽  
Distribution Width ◽  
Hemorrhagic Enteritis ◽  
And Control

Background: Some blood parameters have diagnostic and prognostic importance for the infections in human medicine. However, there is insufficient research regarding the importance of blood parameters and their correlations in veterinary medicine. Increased blood cell distribution width (RDW) and platelet activity can link with the important inflammatory markers. The main objective of the present study was the evaluation of the relationship among some important blood parameters namely RDW, platelet count (PLT), platelet distribution width (PDW), mean platelet volume (MPV), plateletcrit (PCT), their potential usage in the diagnosis and determination of the clinical severity in dogs with hemorrhagic enteritis.Materials, Methods & Results: In this study, the case records of 29 dogs with hemorrhagic enteritis were evaluated and the records of 10 healthy dogs were used as controls. The animals of the study group were presented at the Ondokuz Mayis University, Veterinary Internal Medicine Clinic. The complete blood count (CBC), which includes the total WBC, RBC, hematocrit (HCT), hemoglobin concentration (Hgb), MCV, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), RDW, PLT, MPV, PCT, and PDW, was determined. Significant positive correlations between RDW and RBC, HCT, MCHC, PLT and PDW, and a negative correlation with MCV, were determined. PDW was positively correlated with the lymphocyte count, MCHC and RDW, and negatively correlated with PCT. PLT was negatively correlated with MCV and MPV and positively correlated with RBC and RDW. In addition, MPV was positively correlated with MCV and MCH, and negatively correlated with PLT. Furthermore, there were significant differences between the granulocyte, WBC, HCT, RDW and PDW values (P < 0.001) and monocyte count, Hgb and MCV (P < 0.05), of the study and control groups.Discussion: Acute hemorrhagic enteritis has various causes in dogs such as idiopathic hemorrhagic gastroenteritis and a number of viral, bacterial and parasitic agents. Hematological and biochemical parameters are not specific to enteric diseases, but these paremeters can provide clinically helpful information for differential diagnosis, response to treatment, and prognosis. In this frame, the evaluation of MCV and RDW in combination, and the determination of the mean red cell size and the extent of heterogeneity of the red cell population, can be especially useful to the diagnosis of different red blood cell disorders. In the present study, differences in RDW and MCV values were statistically significant between the study and control groups (P < 0.05). Increased RDW and decreased MCV can be good indicators of hemorragic diseases and in the present study, in addition to these findings, decreased Hgb and Hct confirmed anemia in dogs with hemorrhagic enteritis. The other key findings of this study were statistically significant relationships between RDW, PLT and PDW, which could be important indicators of inflammation in dogs with hemorrhagic enteritis. These parameters should be evaluated carefully in clinical cases of hemorrhagic enteritis. However, due to nature of retrospective studies, there were some limitations (the lack of another control group of dogs suffering from other hemorrhagic diseases) lack of serial measurements of the blood parameters and further studies should be carried out on dogs with hemorrhagic enteritis for a more detailed evaluation and confirmation of the findings of this study.

scholarly journals Impacts of Mycoplasma loads and lung lesions on immune and hematological statuses of pigs in an eight-breed cross heterogeneous population

2020 ◽  
Vol 98 (8) ◽  
Author(s):  
Qing Zhang ◽  
Jing Li ◽  
Tao Huang ◽  
Yifeng Zhang ◽  
Wenwu Xu ◽  
...  
Keyword(s):  
Blood Cell ◽  
Neutrophil Count ◽  
Lymphocyte Count ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Volume Distribution ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Distribution Width ◽  
Lung Lesions

Abstract Developments of pulmonary diseases, often accompanied by infections of bacteria, severely affect the meat production and welfare of pigs. This study investigated 307 pigs at age of 240 d from an eight-breed cross reared under standardized housing conditions for associations among the extent of lung lesions, bacteria load inferred from 16S rRNA sequencing of bronchoalveolar lavage fluid, as well as 57 immune cells and 25 hematological traits. We showed that the pigs under study suffered substantial and varied lung lesions, and the Mycoplasma is the most associated bacteria genera. At a false discovery rate of 0.05 (FDR &lt; 0.05), the severity of lung lesions were significantly associated with greater CD8+ to CD3+ cell ratio, neutrophil-to-lymphocyte ratio (NLR), and standard deviation of red blood cell volume distribution width (RDW-SD), and lower CD4−CD8−/CD3+, CD3+CD4−CD8−/PBMCs (peripheral blood mononuclear cells) and CD14−CD16−/PBMCs cell ratios, mean corpuscular hemoglobin concentration, lymphocyte count, and lymphocyte count percentage, reflecting an status of inflammation, immune suppression, and hypoxia of the pigs accompanying the progression of the lung lesions. The Mycoplasma abundance showed positive correlations with neutrophil count, neutrophil count percentage, NLR, monocyte count, coefficient of variation in red blood cell volume distribution width , and RDW-SD, and negative correlations with mean corpuscular hemoglobin concentration, lymphocyte count, and lymphocyte count percentage; these correlations are largely consistent with those of lung lesions, supporting the comorbidity of lung lesions and Mycoplasma infection. We also observed nonlinear associations that sharp increases in neutrophil count and neutrophil count percentage occurred only when Mycoplasma abundance raised above the population-average level. The results provide helpful insights into the changes of host immune status in response to Mycoplasma relevant lung diseases in pigs.

scholarly journals Effect of Resistance Training on Hematological Blood Markers in Older Men and Women: A Pilot Study

2009 ◽  
Vol 2009 ◽  
pp. 1-4 ◽  
Author(s):  
Florian Bobeuf ◽  
Mélissa Labonté ◽  
Abdelouahed Khalil ◽  
Isabelle J. Dionne
Keyword(s):  
Resistance Training ◽  
Large Population ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Mean Corpuscular Hemoglobin ◽  
Older Individuals ◽  
Corpuscular Hemoglobin ◽  
Distribution Width ◽  
Men And Women ◽  
Significant Difference

The aim of this study was to examine the effects of resistance training on hematological blood markers in older individuals. Twenty-nine men and women participated to this study. Subjects were randomized in 2 groups: (1) control (n=13) and (2) resistance training (n=16). At baseline and after the intervention, subjects were submitted to a blood sample to determine their hematological profile (red blood cells, hemoglobin, hematocrit, platelets, leukocytes, neutrophils, lymphocytes, monocytes, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, red cell distribution width). At baseline, no difference was observed between groups. Moreover, we found no significant difference after the intervention on any of these markers. A 6-month resistance program in healthy older individuals seems to have no beneficial nor deleterious effects on hematological blood parameters. However, resistance training was well tolerated and should be recommended for other health purposes. Further studies are needed to confirm these results in a large population.

scholarly journals Complete Blood Count Peculiarities in Pregnant SARS-CoV-2-Infected Patients at Term: A Cohort Study

Diagnostics ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 80
Author(s):  
Roxana Covali ◽  
Demetra Socolov ◽  
Razvan Socolov ◽  
Ioana Pavaleanu ◽  
Alexandru Carauleanu ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Blood Count ◽  
Complete Blood Count ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Blood Cell Count ◽  
Corpuscular Hemoglobin ◽  
Distribution Width

Background: During viral outbreaks, pregnancy poses an increased risk of infection for women. Methods: In a prospective study, all patients admitted for delivery at term to Elena Doamna Obstetrics and Gynecology University Hospital in Iasi, Romania, between 1 April 2020 and 31 December 2020 were included. There were 457 patients, divided into two groups: group 1, SARS-CoV-2-positive patients (n = 46) and group 2, SARS-CoV-2-negative patients (n = 411). Among other tests, complete blood count was determined upon admittance, and the following values were studied: white blood cell count, lymphocytes, neutrophils, red blood cell count, hemoglobin, mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, mean corpuscular volume, red blood cell distribution width, hematocrit, platelet count, mean platelet volume, platelet distribution width, plateletcrit, and platelet large cell ratio. Results: in pregnant SARS-CoV-2-infected patients at term, there was a significant decrease in white blood cell, neutrophil, and lymphocyte count, and an increase in mean corpuscular hemoglobin concentration, compared to healthy pregnant women at term, although all still within normal limits. None of the other components of the complete blood count or fetal outcomes studied was significantly influenced by SARS-CoV-2 infection in pregnant patients at term.

Impact of weight loss on inflammation and red blood cell biomarkers after laparoscopic gastric banding surgery

2017 ◽  
Vol 66 (2) ◽  
pp. 304-308 ◽  
Author(s):  
Susana Coimbra ◽  
Cátia Ferreira ◽  
Luís Belo ◽  
Petronila Rocha-Pereira ◽  
Alice Catarino ◽  
...  
Keyword(s):  
Weight Loss ◽  
Blood Cell ◽  
Gastric Banding ◽  
Iron Metabolism ◽  
Red Blood Cell ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Obese Patients ◽  
Corpuscular Hemoglobin ◽  
Distribution Width

Adipose tissue produces several adipokines that are enrolled in different metabolic and inflammatory pathways that may disturb iron metabolism and erythropoiesis. Considering that laparoscopic adjustable gastric banding (LAGB) has not been associated with a long-term risk of malabsorption, we performed a 13-month follow-up study in severe obese patients submitted to LAGB in order to clarify its impact on inflammation, iron metabolism and on red blood cell (RBC) biomarkers. Twenty obese patients were enrolled in the study, being clinical and analytically assessed before (T0) and 13 months after LAGB intervention (T1). Inflammation, iron bioavailability and RBC biomarkers were evaluated at T0 and T1. At T1, weight and anthropometric indices decreased significantly; patients showed a significant increase in mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration (MCHC), and a reduction in red cell distribution width, ferritin, hepcidin, tumor necrosis factor-α, interleukin-6 (IL-6) and C-reactive protein. Before LAGB, IL-6 correlated negatively with iron, hemoglobin concentration and MCHC; hepcidin correlated inversely with transferrin. Our data show that 13 months after LAGB, the weight loss is associated with an improvement in inflammation, namely a reduction in IL-6 that may reduce hepcidin production, improving iron availability for erythropoiesis, as shown by more adequate erythrocyte hemoglobinization.

scholarly journals Comparative effect of probiotic (Saccharomyces cerevisiae), prebiotic (Fructooligosaccharide) and their combination on some blood indices in young common carp (Cyprinus carpio L.)

2016 ◽  
Vol 40 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Nasreen M. Abdulrahman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Blood Cell ◽  
Common Carp ◽  
Red Blood Cell ◽  
Blood Parameters ◽  
Average Weight ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Yeast Saccharomyces Cerevisiae ◽  
Blood Indices

     This study was carried out to examine the effect of Fructooligosaccharide as a source of prebiotic, and commercial dry yeast (Saccharomyces cerevisiae) as a source of probiotic and their combination in different level as a source of synbiotic. The experiment was conducted in the fish laboratory of Animal Production Department, Faculty of Agricultural Sciences of Sulaimani University. The trials lasted for three months after 21 days of adapting period 120 common carp fingerlings with an average weight of 20 ±2 gm, were used  to test the effect of different levels of the Fructooligosaccharide, yeast and their combination. In T1 fish were fed a diet with 2.5 gm/kg Fructooligosaccharide, in T2, fish were fed a diet 2.5 gm/kg yeast, T3 represents the third treatment, in which fish were fed on a diet 5 gm/kg Fructooligosaccharide. While, in T4 fish were fed a diet 5 gm/kg yeast, and T5 2.5 Fructooligosaccharide: 2.5 Yeast (gm/kg), T6 was 5 Fructooligosaccharide: 5 yeast (gm/kg), T7 2.5 Fructooligosaccharide: 5 Yeast gm/kg), while T8 5 Fructooligosaccharide, 2.5 (gm/kg) Yeast. Each treatment in three replicates in which five fingerlings common carp were stocked in plastic tanks, which fed the experimental diets twice daily. Blood parameters of tested fish showed significant differences in Red blood cell count (1012 cells/l) in T5 and T7 by 1.235 and 1.260, respectively. Hemoglobin (g/dl) data were 117.000 in the fifth treatment. The mean corpuscular hemoglobin (pg/cell) was 121.400 in T2, 137.850 in T3, 121.050 in T4, and 135.300 in T6; mean corpuscular hemoglobin concentration (g/l) was 924.000, in the seventh treatment, mean corpuscular volume were (fL) 232.500 and 233.050 in T4 and T8 respectively. There were different effects of the treatment in the studied blood parameters in which the level of 2.5 g/kg in both Fructooligosaccharide and dry yeast affect significantly the Red blood cell, White blood cell and Hemoglobin.

scholarly journals Hematology of Wild Caught Dubois’s Tree FrogPolypedates teraiensis, Dubois, 1986 (Anura: Rhacophoridae)

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Madhusmita Das ◽  
Pravati Kumari Mahapatra
Keyword(s):  
Blood Cell ◽  
Blood Serum ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Reference Ranges ◽  
Corpuscular Hemoglobin ◽  
Differential Leukocyte Count ◽  
Rbc Count

Blood was analyzed from eighty (forty males and forty females) adult individuals ofPolypedates teraiensisto establish reference ranges for its hematological and serum biochemical parameters. The peripheral blood cells were differentiated as erythrocytes, lymphocytes, eosinophils, neutrophils, monocytes, basophils, and thrombocytes, with similar morphology to other anurans. Morphology of blood cells did not vary according to sex. The hematological investigations included morphology and morphometry of erythrocytes, morphometry of leucocytes, packed cell volume (PCV), hemoglobin content (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), erythrocyte or red blood cell (RBC) count, leukocyte or white blood cell (WBC) count, differential leukocyte count, and neutrophil to lymphocyte (N/L) ratio. Besides, protein, cholesterol, glucose, urea, uric acid, and creatinine content of blood serum were assayed. Hematological parameters that differed significantly between sexes were RBC count, length and breadth of RBC, neutrophil %, N/L ratio, area occupied by basophils, and diameter of large lymphocyte and eosinophils. The level of glucose, urea, and creatinine in blood serum also significantly differed between sexes.

scholarly journals Markers of Anemia in Children with Type 1 Diabetes

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Ewa Rusak ◽  
Anna Rotarska-Mizera ◽  
Piotr Adamczyk ◽  
Bogdan Mazur ◽  
Joanna Polanska ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Vitamin B12 ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Red Cell ◽  
Cell Distribution ◽  
Corpuscular Hemoglobin ◽  
Distribution Width

Aim. The aim of the study was to assess markers of anemia in type 1 diabetes (T1D) children, compare them to results obtained in the control group, and estimate their relation to BMI SDS. Methods. 94 (59% ♀) T1D children without other autoimmune disorders, aged 12.5 ± 4.1 years, T1D duration: 4.2 ± 3.6 years, HbA1c 7.3 ± 1.5% (57 ± 12.6 mmol/mol). Sex- and age-matched controls (43 children). In all children, anthropometric measurements, the blood count, iron turnover parameters, and vitamin B12 concentration were taken. Results. T1DM children had significantly higher red cell distribution width (RDW) (13.6 versus 12.6%; p<0.001), hepcidin (0.25 versus 0.12 ng/ml; p<0.001), and vitamin B12 concentrations (459 versus 397 pg/ml; p<0.01) and lower TIBC (59.09 versus 68.15 μmol/l; p<0.001) than in the control group. Logistic regression revealed that RDW, TIBC (both p<0.001), and hepcidin (p<0.05) significantly differentiated both groups. In T1DM children, BMI SDS negatively correlated with vitamin B12 (p<0.01) concentration and mean corpuscular hemoglobin concentration (p<0.05) and positively with TIBC (p<0.01) and HbA1c (p<0.001). Conclusions. Patients and controls differed especially in terms of RDW and TIBC. In studied T1DM children, BMI SDS was associated to iron metabolism parameters and vitamin B12 concentration.

scholarly journals Hematological parameters of Hoplias malabaricus (Characiformes: Erythrinidae) parasitized by Monogenea in lagoons in Pirassununga, Brazil

2013 ◽  
Vol 22 (4) ◽  
pp. 457-462 ◽  
Author(s):  
Lincoln Lima Corrêa ◽  
Letícia Cucolo Karling ◽  
Ricardo Massato Takemoto ◽  
Paulo Sérgio Ceccarelli ◽  
Marlene Tiduko Ueta
Keyword(s):  
Condition Factor ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Good Health ◽  
Blood Parameters ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Hoplias Malabaricus ◽  
Relative Condition Factor ◽  
The Mean

Hematology is an important pathological and diagnostic tool. This paper describes the hematological parameters of 76 specimens of Hoplias malabaricus infested with monogenean parasites, which were collected from two lagoons. The hematological parameters included: erythrocyte count (Er), microhematocrit (Hct), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC). The blood parameters showed no significant changes associated with the infestation. Water temperature was found to be negatively correlated with MCV and Hct, with values of rs= −0.52, p<0.0001 and rs= −0.48, p<0.0001, respectively. The mean Relative Condition Factor was Kn=1.01, indicating good health conditions of the fish in these lagoons. No correlation was found between the monogenean infestation and the blood parameters, or between the environmental factors and the monogeneans.

Genome Wide Scan of Complete Blood Count (CBC) Measures Suggests Strong Linkage of Red Blood Cell (RBC) Count to Chromosome 4q25.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 1729-1729 ◽  
Author(s):  
Diana Abbott ◽  
Kai Wang ◽  
Amy Shapiro ◽  
Trudy Burns ◽  
Jorge Di Paola
Keyword(s):  
Linkage Analysis ◽  
Blood Cell ◽  
Complete Analysis ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Distribution Width ◽  
Large Pedigree ◽  
Strong Linkage ◽  
Significant Heritability ◽  
Von Willebrand

Abstract Previous reports suggest that peripheral blood counts are strongly influenced by environmental and genetic determinants; however few of the genetic factors that regulate these quantitative traits have been discovered. We analyzed CBC data from 395 samples collected from an 854-member Amish pedigree with von Willebrand disease. 71 individuals of the pedigree are heterozygous for a missense mutation at position 4120, represented by a single base substitution (C&gt;T) that predicts an arginine to cysteine change at position 1374 (R1374C) in the A1 domain of the mature von Willebrand factor molecule. The detection of genetic signals is likely to be enhanced in groups that live in a more homogeneous environment like the Amish. Linear and quadratic age effect accounted for varying proportions of the gender-specific variation in the CBC measures (from 1% to 70%). The variance component associated with additive polygenic effects was estimated for each CBC phenotype using MENDEL to obtain estimates of heritability. Significant heritability was found for platelet (h2= 0.518, p &lt;0.0005), white blood cell (WBC) (h2=0.395, p &lt;0.0001), RBC counts (h2= 0.358, p &lt;0.0025) and mean corpuscular hemoglobin concentration (MCHC) (h2= 0.547, p &lt;0.0005). Lower heritability was found for red cell distribution width (RDW) (h2= 0.217, p &lt;0.001) and mean corpuscular hemoglobin (MCH) (h2= 0.231, p &lt; 0.02) hematocrit (Hct) (h2= 0.126, p &lt;0.10) and hemoglobin (Hb) (h2= 0.055, p &gt; 0.2). Interestingly since significant heritability for Hct and Hb was reported in other studies, and to rule out the significant effect of bleeding due to the VWF mutation, we estimated heritability of Hb excluding all members of the pedigree that exhibited the 4120 C&gt;T mutation, h2= 0. 21 (p &lt; 0.05). A primary genetic screen at a 10 cM average interval was performed on the entire Amish pedigree in collaboration with the Marshfield genotyping center. Standard Screening Set 16 with 400 short tandem repeat polymorphic (STRP) markers was utilized generating a total of approximately 160,000 genotypes on the 395 samples. Due to data complexity, the Markov Chain Monte Carlo (MCMC)-based program LOKI was used to conduct multipoint linkage analysis for eight CBC measures that were adjusted for age, sex and mutation status to control for potential confounding. The outcome of interest from the MCMC analysis is represented by the Bayes factor (BF) which examines the probability of linkage in complex pedigrees at every centimorgan (cM). A complete analysis of the genome scan with 1,000,000 iterations was performed. A quantitative trait locus (QTL) suggesting strong linkage was identified for the RBC measure at position 4q25 with a BF of 58.17 at 114.5 cM. This result was confirmed by splitting the pedigree into several smaller subsets and conducting traditional linkage analysis using MERLIN (all individuals in the pedigree were included in at least one subset) with a LOD score of 0.66 (p &lt; 0.05). Interestingly, a previous twin study found evidence for linkage for RBC to 4q32. Similar to animal studies, no evidence of linkage was observed in chromosomal regions known to contain the genes that encode for the hemoglobin chains, erythropoietin or erythropoietin receptor. This analysis in a very large pedigree identified a region of strong linkage for RBC that will be analyzed at higher resolution for the presence of novel modifying genes and alleles that may potentially be important for our understanding of the control of erythropoiesis.

Sign in / Sign up

Export Citation Format

Share Document