scholarly journals Doubling the Chromosome Number of Black Poplar Trees (Populus nigra L.) by Colchicine In vitro

2014 ◽  
Vol 25 (5) ◽  
pp. 1-16
Author(s):  
Ammar Z. Kassab-Bashi ◽  
Nathim Th. Saieed ◽  
Omar M. Omar
Keyword(s):  
Chromosome Number ◽  
Populus Nigra ◽  
Black Poplar ◽  
Poplar Trees

scholarly journals In vitro and in vivo effect of poplar bud (Populi gemma) Extracts on late blight (Phytophthora infestans)

2014 ◽  
Vol 6 (1) ◽  
pp. 5-12
Author(s):  
János Bálint ◽  
Botond Turóczi ◽  
István Máthé ◽  
Klára Benedek ◽  
Károly-Attila Szabó ◽  
...  
Keyword(s):  
Growth Rate ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Environmentally Friendly ◽  
Populus Nigra ◽  
Field Conditions ◽  
Black Poplar

Abstract The effect of populin extract from black poplar (Populus nigra) on late blight was assessed under laboratory and field conditions. The growth rate of hyphae was found to be significantly lower after 1v/v% populin application, and no hyphae growth was detected under 3 and 6v/v% populin application. Populin also reduced the light blight severity on potato leaves under field conditions. From our results, we have concluded that populin extract can be considered as a new and environmentally-friendly alternative for the control of late blight under field conditions.

scholarly journals Micropropagation of Black Poplar Trees ( Populus nigra L. ) by Tissue Culture

2013 ◽  
Vol 24 (12) ◽  
pp. 1-17
Author(s):  
Omar M. Omar ◽  
Ammar Z. Kassab-Bashi ◽  
Nathim Th. Saieed
Keyword(s):  
Tissue Culture ◽  
Populus Nigra ◽  
Black Poplar ◽  
Poplar Trees

scholarly journals In vitro propagation and cytological analysis of Sophora mollis Royle: an endangered medicinal shrub

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Aakriti Bhandari ◽  
Harminder Singh ◽  
Amber Srivastava ◽  
Puneet Kumar ◽  
G. S. Panwar ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Average Length ◽  
Germplasm Conservation ◽  
Basic Chromosome Number ◽  
Shoot Tip ◽  
Ex Situ ◽  
Ms Medium ◽  
Basic Chromosome ◽  
Shoot Tip Explants

Abstract Background Sophora mollis Royle (family Fabaceae, subfamily-Papilionaceae) is a multipurpose legume distributed in plains and foothills of the North-West Himalaya to Nepal and is facing high risk of extinction due to habitat loss and exploitation by the local people for its fuel and fodder values. Therefore, the present study was conducted to standardize a micropropagation protocol for Sophora mollis by using shoot tip explants and to study the meiotic chromosome count in the species. Results Multiple shoots were induced in shoot tip explants of Sophora mollis in Murashige and Skoog medium supplemented with different concentrations of cytokinins alone (BAP, TDZ, and Kinetin) and in combination with varying concentrations of NAA. MS medium supplemented with BAP (8.9 μM) was observed to be the optimal medium for multiple shoot induction and maximum 25.32 shoots per explant was obtained with average length of 4.5 ± 0.8 cm. In vitro developed shoots were transferred onto rooting media supplemented with different concentrations of auxin (IAA, IBA, and NAA). Maximum 86% rooting was observed in half-strength MS medium supplemented with 21.20 μM NAA with an average of 21.26 roots per culture. In vitro raised plantlets were adapted to greenhouse for better acclimatization and 60% plants were successfully transferred to the open environment. Based on the chromosome counts available from the literature and the current study, the species tend to show a basic chromosome number of x = 9. Conclusion The micropropagation protocol standardized can be helpful for the ex situ mass multiplication and germplasm conservation of the endangered species. Moreover, the ex situ conservation approach will be helpful in actively bridging the gap between ex situ and in situ approaches through the reintroduction of species in the wild. The cytological studies revealed the basic chromosome number x = 9 of the species.

Period of harvest, sprouting ability of cuttings, and in vitro plant regeneration in Fraxinus excelsior

1994 ◽  
Vol 72 (2) ◽  
pp. 261-267 ◽  
Author(s):  
Conceição Eneida Silveira ◽  
Alain Cottignies
Keyword(s):  
Chromosome Number ◽  
In Vitro Culture ◽  
Adventitious Roots ◽  
Woody Plant ◽  
Fraxinus Excelsior ◽  
Stem Cuttings ◽  
Natural Conditions ◽  
Apical Bud ◽  
Woody Plant Medium

Propagation by stem cuttings and in vitro culture of apical bud explants were studied on Fraxinus excelsior L. Stem cuttings from 4- to 7-year-old trees growing under natural conditions sprouted only when cuttings were taken from dormant material. Only 6% of those that had sprouted developed roots by the 7th month of culture. Similarly, only apical bud explants harvested during the dormant period sprouted in vitro. Up to 87% of these sprouts developed two to four branching adventitious roots after 5 months of culture. During the initial phase of in vitro culture, the Quoirin and Lepoivre medium and the woody plant medium favoured sprout lengthening. During the phase of multiplication, up to three sprouts per explant developed with the woody plant medium in the presence of a combination of high 6-benzylaminopurine (3.0–4.0 mg∙L−1) and low indole-3-butyric acid (0.01–0.03 mg∙L−1) concentrations. Rooting was obtained in a medium without any growth regulators. Microscopic analysis showed a direct connection between the vascular elements of adventitious roots and stem of plantlet. Chromosome number in root apices of ash plantlets and ash trees grown under natural conditions was 2n = 46. Key words: chromosome number, Fraxinus excelsior L., in vitro plants, micropropagation, stem cuttings.

scholarly journals Improvement Of Orchid Vanda Hybrid (Vanda limbata Blume X Vanda tricolor Lindl. var. suavis) By Colchicines Treatment In Vitro

2016 ◽  
Vol 10 (11) ◽  
pp. 83 ◽  
Author(s):  
Mustika Tuwo ◽  
Ari Indrianto
Keyword(s):  
Chromosome Number ◽  
Morphological Characters ◽  
Effective Concentration ◽  
Cytological Analysis ◽  
Stomatal Index ◽  
Duration Of Treatment ◽  
Stomatal Size ◽  
Soaking Time ◽  
Anatomical Characters

The purpose of this research was to evaluate the growth phase of orchid Vanda hybrid (Vanda limbata Blume X Vanda tricolor Lindl. var. suavis) response with colchicines treatment, to determine precise effective concentration of colchicines and duration of treatment to induce polyploidy. This research used variation of colchicines concentration which were 0.01%; 0.05%; 0.1%; 0.5%; 1% and 0% and incubation for 6 hours; 12 hours; 18 hours; 24 hours; and  4 days. Treatment was done in aseptic condition using protocorm aged 7 week after sowing. After treatments, plants were planted on Vacint and Went (VW) medium supplemented with of 150 ml/l coconut water. After four months, they were subcultured in medium VW + 150 ml/l + 150g/l banana extract. The results showed that the orchid Vanda hybrid was intolerant to colchicine concentration of 0.1; 0.5 and 1% by death protocorm was more than 50%. Concentration of 0,5% with 6 hours soaking time is the most effective to induced tetraploid in orchid Vanda hybrid. Morphological characters of tetraploid plantlets had lower average number and length of roots, the number, length, and width of the leaves than those of control. Anatomical characters of the tetraploid plantlets revealed the highest stomatal size and had smaller of stomatal index than controls. This evident indicated a negative correlation between stomatal index and ploidy level. Cytological analysis with flow cytometry revealed that tetraploid plantlets (2n=4x=76) stained with DAPI showed the chromosome number more than those of control (2n=2x=38).Key words      : polyploidization, colchicine, vanda orchid, chromosome number

Structure of genetic diversity in marginal populations of black poplar (Populus nigra L.)

2015 ◽  
Vol 61 ◽  
pp. 297-302 ◽  
Author(s):  
Dechun Jiang ◽  
Guili Wu ◽  
Kangshan Mao ◽  
Jianju Feng
Keyword(s):  
Genetic Diversity ◽  
Populus Nigra ◽  
Marginal Populations ◽  
Black Poplar

scholarly journals Breast Tumor Cell Lines From Pleural Effusions2

1974 ◽  
Vol 53 (3) ◽  
pp. 661-674 ◽  
Author(s):  
R. Cailleau ◽  
R. Young ◽  
M. Olivé ◽  
W. J. Reeves
Keyword(s):  
Chromosome Number ◽  
Tumor Cell ◽  
Tumor Cells ◽  
Cell Lines ◽  
Breast Tumor ◽  
Tumor Cell Lines ◽  
Breast Cancer Patients ◽  
Pleural Effusions ◽  
Epithelial Tumor

Summary During 1973, 4 new epithelial tumor cell lines were isolated from pleural effusions from breast cancer patients. We describe 3 of these lines: MDA-MB-134, with a mean chromosome number of 43; MDA-MB-175, with a mean chromosome number of 49; and MDA-MB-231, with a mean chromosome number between 65 and 69. We isolated the same cell type from 4 of 10 effusions from MDA-MB-134 and from 6 of 8 effusions from MDA-MB-175. We found that pleural effusions as a source of breast tumor cells to be cultured and studied in vitro have the following advantages: 1) large amounts of material and the possibility of obtaining sequential samples from the same patient; 2) high viability of tumor cells; 3) scarcity or absence of fibroblasts; and 4) the possibility of separating the tumor cells from other “contaminating” cell types by differences in their speed or degree of attachment to the flask. All lines from different patients differed, as seen grossly and microscopically. All lines from sequential pleural effusions from the same patient were apparently alike. No viruses or mycoplasmas were detected in any line.

ANTIMICROBIAL ACTIVITY OF BLACK POPLAR (POPULUS NIGRA L.) BUD AND SPROUT TINCTURES

2019 ◽  
Vol 68 (3) ◽  
Author(s):  
E.A. Kupriyanova ◽  
V.A. Kurkin ◽  
V.M. Ryzhov ◽  
A.V. Lyamin ◽  
O.V. Kondratenko ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Populus Nigra ◽  
Black Poplar
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