scholarly journals In vitro and in vivo effect of poplar bud (Populi gemma) Extracts on late blight (Phytophthora infestans)

2014 ◽  
Vol 6 (1) ◽  
pp. 5-12
Author(s):  
János Bálint ◽  
Botond Turóczi ◽  
István Máthé ◽  
Klára Benedek ◽  
Károly-Attila Szabó ◽  
...  
Keyword(s):  
Growth Rate ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Environmentally Friendly ◽  
Populus Nigra ◽  
Field Conditions ◽  
Black Poplar

Abstract The effect of populin extract from black poplar (Populus nigra) on late blight was assessed under laboratory and field conditions. The growth rate of hyphae was found to be significantly lower after 1v/v% populin application, and no hyphae growth was detected under 3 and 6v/v% populin application. Populin also reduced the light blight severity on potato leaves under field conditions. From our results, we have concluded that populin extract can be considered as a new and environmentally-friendly alternative for the control of late blight under field conditions.

scholarly journals In Vitro and In Vivo Effect of Poplar Bud Extracts on Phytophthora infestans: A New Effective Biological Method in Potato Late Blight Control

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 217 ◽  
Author(s):  
Botond Turóczi ◽  
József Bakonyi ◽  
Károly-Attila Szabó ◽  
János Bálint ◽  
István Máthé ◽  
...  
Keyword(s):  
Growth Rate ◽  
Late Blight ◽  
Low Cost ◽  
Infection Intensity ◽  
Field Conditions ◽  
Biological Method ◽  
Environmentally Friendly Approach ◽  
Open Field Condition

The effect of populin extract from black poplar (Populus nigra) on seven different late blight strains was tested under laboratory and field conditions. The growth rate of hyphae was found to be significantly lower in vitro after 3 and 4 v/v% populin applications. Stain M16 was resistant to populin treatment under lab conditions, however. Both 5% and 10% concentration populin reduced the M16 strain’s severity on potato leaves under field conditions and proved to be even more effective than conventionally used fungicides Infinito 687 Sc and Valis M. Higher infection intensity at the 1% level was observed after 24 h using Valis M, and the same trend toward 10% infection remained after 48 and 72 h as well. Low, almost-no-infection intensity was detected after populin 5% and 10% treatment under an open field condition. Altogether, it can be concluded that populin extract can be a low-cost option for growers and an environmentally friendly approach in late blight control.

scholarly journals The Effectiveness of Induced Defense Responses in a Susceptible Potato Genotype Depends on the Growth Rate of Phytophthora infestans

2019 ◽  
Vol 32 (1) ◽  
pp. 76-85 ◽  
Author(s):  
Cécile Thomas ◽  
Romain Mabon ◽  
Didier Andrivon ◽  
Florence Val
Keyword(s):  
Growth Rate ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Induced Defense ◽  
Plant Immunity ◽  
Defense Responses ◽  
Alternative Methods ◽  
Gene Expressions

Phytophthora infestans causes the devastating potato late blight disease, which is widely controlled with fungicides. However, the debate about chemical control is fueling a promotion toward alternative methods. In this context, the enhancement of natural plant immunity could be a strategy for more sustainable protection. We previously demonstrated that a concentrated culture filtrate (CCF) of P. infestans primes defense reactions in potato. They are genotype-dependent and metabolites produced decrease pathogen growth in vitro but not in vivo on tubers. Induced potato defenses are assumed to affect P. infestans life history traits depending on strains. This assumption was studied in vivo through induced leaflets on a susceptible genotype inoculated with four P. infestans strains differing for lesion growth rate. This study combines both defenses mechanistic analysis and ecological observations. Defense-gene expressions were thus assessed by quantitative reverse transcription-polymerase chain reaction; pathogen development was simultaneously evaluated by measuring necrosis, quantifying mycelial DNA, and counting sporangia. The results showed that CCF pretreatment reduced the pathogenicity differences between slow- and fast-growing strains. Moreover, after elicitation, PR-1, PR-4, PAL, POX, and THT induction was strain-dependent. These results suggest that P. infestans could develop different strategies to overcome plant defenses and should be considered in biocontrol and epidemic management of late blight.

scholarly journals Respuesta de biosurfactantes producidos por Pseudomonas fluorescens para el control de la gota de la papa Phytophthora infestans (Mont) de Bary, bajo condiciones controlada.

2010 ◽  
Vol 11 (1) ◽  
pp. 21
Author(s):  
Hugo F. Rivera ◽  
Erika P. Martínez ◽  
Jairo A. Osorio ◽  
Edgar Martínez
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Pseudomonas Fluorescens ◽  
Negative Impact ◽  
Potato Late Blight ◽  
Pathogen Resistance ◽  
Production Costs ◽  
In Vitro Assays

<p>Phytophthora infestans (Mont.) de Bary, agente causal de la gota de la papa, es considerado la principal limitante de la producción de este cultivo en Colombia. El control habitual del patógeno se realiza con fungicidas de tipo sistémico, que incrementan los costos de producción, pueden inducir la resistencia del patógeno y tiene un impacto negativo en el ambiente. Por tanto, se llevó a cabo este estudio con el propósito de buscar alternativas amigables con el ambiente, que hagan parte de un paquete tecnológico eficaz de control. Dos cepas nativas de Psedomonas fluorescens (039T y 021V), provenientes de cultivos de papa, fueron evaluadas contra P. infestans. Las suspensiones bacterianas y los biosurfactantes parcialmente purificados (BPP), producidos por éstas (obtenidos en medio mínimo de sales con querosén), fueron aplicados sobre foliolos desprendidos en ensayos in vitro y experimentos in vivo en plantas de papa, en condiciones controladas en casa de malla. Los resultados demostraron la capacidad que tienen los biosurfactantes y las suspensiones bacterianas para controlar al patógeno, ya que el BPP 039T logró reducir el nivel de severidad de la enfermedad en 79,9% in vitro y 38,5% in vivo, mientras que el BPP 021V redujo en 78,7% in vitro y 30,2% in vivo. Las suspensiones bacterianas redujeron el nivel de severidad en 72,4% (039T) y 66,1% (021V) en las evaluaciones in vitro y 35% en los experimentos in vivo. Los resultados de esta investigación muestran el potencial que tienen los biosurfactantes para el control de la gota en Colombia.</p><p> </p><p><strong>Evaluation of Biosurfactants Produced by Pseudomonas fluorescens for Potato Late Blight Control (Phytophthora infestans (Mont) de Bary) Under Controlled Conditions</strong></p><p>Phytophthora infestans (Mont.) de Bary, causal agent of potato late blight is considered the main limiting pathogen for the production of this crop in Colombia. The usual control of the disease has been performed with systemic fungicides which increase production costs, can induce pathogen resistance and have a negative impact on the environment. Therefore, this study was carried out in order to find effective and environmentally friendly control alternatives for potato late blight. Two Pseudomonas fluorescens native strains (039T and 021V) isolated from potato crops were evaluated against P. infestans. Bacterial suspensions (obtained from minimal salts medium added with kerosene) and partially purified biosurfactants (BPP) were applied on detached leaflets for in vitro assays and on potato plants in greenhouse, for in vivo assays and the measure of inhibitory effect of the disease was assessed. The results showed the ability of P. fluorescens biosurfactants and bacterial suspensions to control the pathogen. BPP 039T was able to reduce the level of severity disease by 79.9% in vitro and 38.5% in vivo, whereas BPP 021V decreased 78.7% in vitro and 30.2% in vivo. Bacterial suspensions reduced the severity level in 72.4% (039T) and 66.1% (021V) in vitro assessments and 35% in the in vivo experiment. These results show the potential of P. fluorescens biosurfactants to control the potato late blight in Colombia.</p>

Accumulation of hypoxanthine in potato leaves and its effect on spore germination in Phytophthora infestans

1968 ◽  
Vol 46 (3) ◽  
pp. 267-270 ◽  
Author(s):  
M. C. Clark ◽  
O. T. Page
Keyword(s):  
Aqueous Solution ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Spore Germination ◽  
Detached Leaves ◽  
Whole Plants

The accumulation of hypoxanthine (6-hydroxypurine) in blighted potato leaves and its effect on spore germination in the late blight organism, Phytophthora infestans, were investigated. Preliminary experiments on the translocation of this compound in whole plants and detached leaves were also conducted.Under in vitro and in vivo conditions, hypoxanthine had a pronounced stimulatory effect on the indirect germination of sporangia. Studies on the movement of this base indicate that it is freely translocated, but fails to be exported from detached leaves into aqueous solution. These observations, coupled with the finding that hypoxanthine readily accumulated in blighted leaves, suggest that this compound could be of importance in predisposing uninfected portions of the host to invasion by the pathogen.

Research of microcystins as inhibitors of Phytophthora infestans development

2021 ◽  
Author(s):  
V. V. Nykyforov ◽  
◽  
О.V. Novokhatko ◽  
O.V. Maznitska ◽  
О.А. Sakun ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Green Algae ◽  
Field Experiments ◽  
Model Organisms ◽  
Time Interval ◽  
Complete Disappearance ◽  
Blue Green Algae

Information from foreign literary sources regarding microcystins of blue-green algae is presented. The current state of the problem of reservoirs «blooming» and the significance of this phenomenon for humans are reflected. The research was carried out in two stages: laboratory and full-scale. A pure culture of Phytophthora infestans was isolated, on which further studies were carried out. Isolation of isolates was carried out on agar nutrient medium. Potassium permanganate and ethanol were selected from the available antiseptics. Field experiments were carried out on experimental lines of Solanum lycopersicum by diagnosing signs of late blight disease. The determination of the potential negative effect of tomato plants treated with a suspension of cyanobacteria was carried out by the method of biotesting using Achatina fulica as a test object. The effect of blue-green algae toxins – microcystins – on colonies Ph. infestans in vitro is described. Photometric observation of the decrease in the number of colonies with a time interval of three days is presented. The phytophtorostatic effect of microcystins has been established. Degradation of Ph. infestans the next day were fixed after treatment of the colony with a suspension of cyanobacteria. An inhibitory effect was revealed, almost to the complete disappearance of symptoms in plants partially affected by late blight and grown in vivo. It has been determined that plants treated with microcystin can be considered safe for further consumption; the death of model organisms has not been registered.

scholarly journals In Vitro and In Vivo Antifungal Activity of Sorbicillinoids Produced by Trichoderma longibrachiatum

2021 ◽  
Vol 7 (6) ◽  
pp. 428
Author(s):  
Men Thi Ngo ◽  
Minh Van Nguyen ◽  
Jae Woo Han ◽  
Myung Soo Park ◽  
Hun Kim ◽  
...  
Keyword(s):  
Late Blight ◽  
Culture Filtrate ◽  
Fungal Pathogens ◽  
Gray Mold ◽  
Trichoderma Longibrachiatum ◽  
Chemical Structures ◽  
Natural Agents ◽  
Blight Disease

In the search for antifungal agents from marine resources, we recently found that the culture filtrate of Trichoderma longibrachiatum SFC100166 effectively suppressed the development of tomato gray mold, rice blast, and tomato late blight. The culture filtrate was then successively extracted with ethyl acetate and n-butanol to identify the fungicidal metabolites. Consequently, a new compound, spirosorbicillinol D (1), and a new natural compound, 2′,3′-dihydro-epoxysorbicillinol (2), together with 11 known compounds (3–13), were obtained from the solvent extracts. The chemical structures were determined by spectroscopic analyses and comparison with literature values. The results of the in vitro antifungal assay showed that of the tested fungal pathogens, Phytophthora infestans was the fungus most sensitive to the isolated compounds, with MIC values ranging from 6.3 to 400 µg/mL, except for trichotetronine (9) and trichodimerol (10). When tomato plants were treated with the representative compounds (4, 6, 7, and 11), bisvertinolone (6) strongly reduced the development of tomato late blight disease compared to the untreated control. Taken together, our results revealed that the culture filtrate of T. longibrachiatum SFC100166 and its metabolites could be useful sources for the development of new natural agents to control late blight caused by P. infestans.

scholarly journals In Vivo Radioprotective Activity of Cell-Permeable Bifunctional Antioxidant Enzyme GST-TAT-SOD against Whole-Body Ionizing Irradiation in Mice

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Jianru Pan ◽  
Huocong He ◽  
Ying Su ◽  
Guangjin Zheng ◽  
Junxin Wu ◽  
...  
Keyword(s):  
Growth Rate ◽  
Antioxidant Activity ◽  
Body Weight ◽  
Whole Body ◽  
White Pulp ◽  
Radioprotective Activity ◽  
Significant Difference ◽  
Wbc Count

GST-TAT-SOD was the fusion of superoxide dismutase (SOD), cell-permeable peptide TAT, and glutathione-S-transferase (GST). It was proved to be a potential selective radioprotector in vitro in our previous work. This study evaluated the in vivo radioprotective activity of GST-TAT-SOD against whole-body irradiation. We demonstrated that intraperitoneal injection of 0.5 ml GST-TAT-SOD (2 kU/ml) 2 h before the 6 Gy whole-body irradiation in mice almost completely prevented the splenic damage. It could significantly enhance the splenic antioxidant activity which kept the number of splenic white pulp and consequently resisted the shrinkage of the spleen. Moreover, the thymus index, hepatic antioxidant activity, and white blood cell (WBC) count of peripheral blood in irradiated mice pretreated with GST-TAT-SOD also remarkably increased. Although the treated and untreated irradiated mice showed no significant difference in the growth rate of animal body weight at 7 days postirradiation, the highest growth rate of body weight was observed in the GST-TAT-SOD-pretreated group. Furthermore, GST-TAT-SOD pretreatment increased resistance against 8 Gy whole-body irradiation and enhanced 30 d survival. The overall effect of GST-TAT-SOD seemed to be a bit more powerful than that of amifostine. In conclusion, GST-TAT-SOD would be a safe and potentially promising radioprotector.

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