scholarly journals Resilience Capacity Assessment of the Traditional Lima Bean (Phaseolus lunatus L.) Landraces Facing Climate Change

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 758 ◽  
Author(s):  
María Isabel Martínez-Nieto ◽  
Elena Estrelles ◽  
Josefa Prieto-Mossi ◽  
Josep Roselló ◽  
Pilar Soriano
Keyword(s):  
Climate Change ◽  
Phylogenetic Analyses ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Time Base ◽  
Base Temperature ◽  
Seed Traits ◽  
South American Species ◽  
Commercial Cultivar ◽  
Water Stress Tolerance

Agriculture is highly exposed to climate warming, and promoting traditional cultivars constitutes an adaptive farming mechanism from climate change impacts. This study compared seed traits and adaptability in the germinative process, through temperature and drought response, between a commercial cultivar and Mediterranean Phaseolus lunatus L. landraces. Genetic and phylogenetic analyses were conducted to characterize local cultivars. Optimal germination temperature, and water stress tolerance, with increasing polyethylene glycol (PEG) concentrations, were initially evaluated. Base temperature, thermal time, base potential and hydrotime were calculated to compare the thermal and hydric responses and competitiveness among cultivars. Eight molecular markers were analyzed to calculate polymorphism and divergence parameters, of which three, together with South American species accessions, were used to construct a Bayesian phylogeny. No major differences were found in seed traits, rather different bicolored patterns. A preference for high temperatures and fast germination were observed. The ‘Pintat’ landrace showed marked competitiveness compared to the commercial cultivar when faced with temperature and drought tolerance. No genetic differences were found among the Valencian landraces and the phylogeny confirmed their Andean origin. Promoting landraces for their greater resilience is a tool to help overcome the worldwide challenge deriving from climate change and loss of agrobiodiversity.

scholarly journals Comprehensive genomic resources related to domestication and crop improvement traits in Lima bean

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Tatiana Garcia ◽  
Jorge Duitama ◽  
Stephanie Smolenski Zullo ◽  
Juanita Gil ◽  
Andrea Ariani ◽  
...  
Keyword(s):  
Climate Change ◽  
Crop Improvement ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Rna Seq ◽  
Sequencing Technologies ◽  
Biparental Population ◽  
Wide Range ◽  
Genomic Analyses ◽  
Intrachromosomal Rearrangements

AbstractLima bean (Phaseolus lunatus L.), one of the five domesticated Phaseolus bean crops, shows a wide range of ecological adaptations along its distribution range from Mexico to Argentina. These adaptations make it a promising crop for improving food security under predicted scenarios of climate change in Latin America and elsewhere. In this work, we combine long and short read sequencing technologies with a dense genetic map from a biparental population to obtain the chromosome-level genome assembly for Lima bean. Annotation of 28,326 gene models show high diversity among 1917 genes with conserved domains related to disease resistance. Structural comparison across 22,180 orthologs with common bean reveals high genome synteny and five large intrachromosomal rearrangements. Population genomic analyses show that wild Lima bean is organized into six clusters with mostly non-overlapping distributions and that Mesomerican landraces can be further subdivided into three subclusters. RNA-seq data reveal 4275 differentially expressed genes, which can be related to pod dehiscence and seed development. We expect the resources presented here to serve as a solid basis to achieve a comprehensive view of the degree of convergent evolution of Phaseolus species under domestication and provide tools and information for breeding for climate change resiliency.

scholarly journals Comprehensive genomic resources related to domestication and crop improvement traits in Lima bean

2020 ◽  
Author(s):  
Tatiana Garcia ◽  
Jorge Duitama ◽  
Stephanie Zullo ◽  
Juanita Gil ◽  
Andrea Ariani ◽  
...  
Keyword(s):  
Climate Change ◽  
Common Bean ◽  
Convergent Evolution ◽  
Developmental Stages ◽  
Crop Improvement ◽  
Genomic Analysis ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Human Consumption ◽  
Gene Pools

Abstract Lima bean (Phaseolus lunatus L.) is one of the five domesticated Phaseolus bean crops, which are essential sources of dietary proteins for human consumption. Compared to common bean (P. vulgaris), it shows a wider range of ecological adaptations along its distribution range from Mexico to Argentina. These adaptations and its phenotypic plasticity make Lima bean a promising crop for improving food security under predicted scenarios of climate change in Latin America and elsewhere. Lima bean is also an excellent model to study convergent evolution of the adaptive domestication syndrome due to its dual domestication in Mesoamerica and the Andes. Combining long and short read sequencing technologies with a dense genetic map from a biparental population, we obtained the first chromosome-level genome assembly for Lima bean. Annotation of 28,326 gene models showed high diversity among 1,917 genes with conserved domains related to disease resistance. Structural comparison across 21,180 orthologs with common bean revealed high genome synteny and two large intrachromosomal rearrangements. Speciation between P. lunatus and P. vulgaris occurred about six million years ago according to nucleotide evolution between these orthologs. Population genomic analysis of GBS data for 482 wild and domesticated accessions from the Mesoamerican and Andean gene pools provided novel evidence on population structure at a finer geographical scale. Results show that wild Lima bean is organized into six clusters with mostly non-overlapping distributions and that Mesomerican landraces can be further subdivided into three subclusters. A new wild cluster of diversity was found in the Colombian Andes and a separate genetic cluster was observed for Mesoamerican landraces of the Peninsula of Yucatan in Mexico. This study also documents genome wide patterns of selection and haplotype introgression events among gene pools. Analysis of RNA-seq data obtained from wild and domesticated accessions at two different pod developmental stages revealed 4,275 differentially expressed genes, which could be related to pod dehiscence and seed development. We expect that the present resources serve as a solid basis to achieve a comprehensive view of the degree of convergent evolution of Phaseolus species under domestication and provide new tools and information for breeding for climate change resiliency of different domesticated species.

Genetic diversity in accessions of lima bean (Phaseolus lunatus L.) determined from agro-morphological descriptors and SSR markers for use in breeding programs in Brazil

2021 ◽  
Author(s):  
Carolline de Jesús Pires ◽  
Marcones Ferreira Costa ◽  
Maria Imaculada Zucchi ◽  
Regina Lucia Ferreira-Gomes ◽  
José Baldin Pinheiro ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Morphological Descriptors ◽  
Breeding Programs

scholarly journals Development of Texturized Vegetable Protein from Lima Bean (Phaseolus lunatus) and African Oil Bean Seed [Pentaclethrama crophylla (Benth)]: Optimization Approach

2017 ◽  
Vol 21 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Gibson. L. Arueya ◽  
Bamidele. S. Owosen ◽  
Kazeem. K. Olatoye
Keyword(s):  
Vegetable Protein ◽  
Control Sample ◽  
Lima Bean ◽  
Essential Amino Acids ◽  
Phaseolus Lunatus ◽  
Optimization Approach ◽  
Bean Seed ◽  
Overall Acceptability ◽  
Extrusion Processing ◽  
Feed Moisture

AbstractAs part of measures to combat protein shortages in form of meat analogues, extrusion processing conditions for the development of Texturized Vegetable Protein (TVP) from under-utilized sources (Lima bean and African oil bean seed) are analysed. Optimum parameters for processing were established as being: barrel temperature (92.45°C), screw speed (101.48 rpm), feed moisture (59.63%) and African oil bean seed protein concentrates (AOBSPC) of 1%. Concentrations of essential amino-acids were also found to be significant (0.90-7.3%) with a near absence of anti-nutritional factors (0.0022–1.0008) g/kg. Sensory evaluation showed that TVP5 (100% LBPC) compared favourably with the control sample (cooked meat) in overall acceptability. An Acceptable and nutritious meat analogue had been developed.

ISOLATION AND CHARACTERIZATION OF LIMA BEAN (PHASEOLUS LUNATUS) STARCH

1991 ◽  
Vol 15 (2) ◽  
pp. 117-136 ◽  
Author(s):  
RATNAJOTHI HOOVER ◽  
STEWART C. RORKE ◽  
ANTONIO M. MARTIN
Keyword(s):  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Isolation And Characterization

scholarly journals Induction of chlorophyll mutation through physical and chemical mutagen in Phaseolus lunatus (Lima Bean)

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Kalyani R Gunjal ◽  
A.D. More
Keyword(s):  
Gamma Rays ◽  
Mutation Frequency ◽  
Lima Bean ◽  
Mutation Breeding ◽  
Phaseolus Lunatus ◽  
Chlorophyll Mutation ◽  
Dose Concentration ◽  
Chlorophyll Mutants ◽  
Physical And Chemical ◽  
Light Green

The present investigation was undertaken to study the frequency of chlorophyll deficient sectors and chlorophyll mutants induced by Gamma rays at the dosage of 240Gy,300Gy,360Gy,420Gy, Ethyl Methene Sulphate (EMS) at the concentrations of 0.25%,0.50%,0.75%,1% and the Combination of both (Gamma rays and EMS) like 240Gy+1%, 300Gy+0.75%, 360Gy+0.50%, 420Gy+0.25% in the variety ‘King of Garden’ Phaseolus lunatus (lima bean).The chlorophyll mutants were observed in all the three mutagenic treatments in the lima bean. These mutants were White (Albina), Yellow (Xantha), Yellow green (Chlorina) and Light green (Viridis). They were screened after 10-15 days of old seedling and were found at the margin of the leaflet or the entire leaflet.Induced mutation in the sectors of the leaves resulted into chlorophyll chimeric plants. The scoring of chlorophyll mutation frequency in M2 generation was one of the most reliable measures for evaluating the mutagenic induced genetic altercation of the mutagen treatments used in the ideotype. The frequency of chlorophyll mutants increased with the increase in the dose/concentration of the mutants. They can be screened and enhanced through mutation breeding for enormous yield.

scholarly journals First Report of Phytophthora capsici Infecting Lima Bean (Phaseolus lunatus) in the Mid-Atlantic Region

Plant Disease ◽  
2002 ◽  
Vol 86 (9) ◽  
pp. 1049-1049 ◽  
Author(s):  
C. R. Davidson ◽  
R. B. Carroll ◽  
T. A. Evans ◽  
R. P. Mulrooney ◽  
S. H. Kim
Keyword(s):  
Phytophthora Capsici ◽  
Petri Dish ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Pathogenicity Test ◽  
Atlantic Region ◽  
Kent County ◽  
Original Field ◽  
Lima Beans ◽  
Stem Lesions

Lima beans are an important crop in Delaware and the Mid-Atlantic Region. In the summer of 2000, five commercial cultivars (3–28, 184–85, C-elite Sel, Butter Bean, and Jackson Wonder) of lima bean in Delaware, Maryland, and New Jersey were observed with white, appressed mycelia on infected pods that appeared distinctly different from signs of downy mildew infection caused by Phytophthora phaseoli. Isolations were made by placing diseased pods between layers of rye media (1). A fungus that produced white mycelia with sporangia was consistently isolated. All Phytophthora isolates from the infected pods were heterothallic, grew at 35°C, had as much as 100 μm long pedicles on varying shapes of caducous sporangia with tapering base and >2 papillae, and were identified as P. capsici (2). Initially, three surface-disinfected pods from cv. Early Thorogreen plants grown in the greenhouse were floated on 20 ml of sterile water in a petri dish, and each was inoculated with a disk of P. capsici. This was repeated for nine isolates obtained from lima bean. After incubation for 7 days at room temperature, all 27 pods were infected, and P. capsici was reisolated from all the pods. A pathogenicity test was performed on the same cultivars from which the original field isolates were collected. Three seedlings and two plants with mature pods were inoculated with a sporangial suspension of each of the nine isolates and placed in a dew chamber for 5 days at 20 to 25°C and 100% relative humidity. White mycelial growth was observed on seedlings and mature pods. One inoculated plant developed brown-to-black stem lesions with white mycelia. All pods on the mature plants showed appressed, white mycelia identical to that observed in the commercial lima bean fields. P. capsici was consistently reisolated from all inoculated plants. In 2000, most infected pods in infested fields were observed low in the plant canopy or touching the soil. However, in 2001, infected pods were mostly in the lower and mid-portion of the plants observed in baby lima bean fields in Kent County, DE. References: (1) C. E. Caten and J. L. Jinks. Can. J. Bot. 46:329, 1967. (2) D. C. Erwin and O. K. Ribeiro. Phytophthora capsici. Page 264 in: Phytophthora Diseases Worldwide. The American Phytopathological Society, St Paul, MN, 1996.

scholarly journals Protein Efficiency Ratio of Pigeon Pea (Cajanus cajan) and Lima Bean (Phaseolus lunatus): A Sprague-Dawley Rat Growth Assay

2018 ◽  
Vol 52 (3) ◽  
Author(s):  
Raycha Lei Concess M. Rama ◽  
Jillean Camille Q. Fabi ◽  
Generose Christy M. Mateo ◽  
Jewel Amor C. Catubag ◽  
Lemuel L. Lozada ◽  
...  
Keyword(s):  
Cajanus Cajan ◽  
Protein Efficiency Ratio ◽  
Lima Bean ◽  
Protein Digestibility ◽  
Pigeon Pea ◽  
Phaseolus Lunatus ◽  
Sprague Dawley ◽  
Efficiency Ratio ◽  
Weanling Rats ◽  
Growth Assay

Objective. This study has been conducted to determine the protein efficiency ratio (PER) of selected indigenous legumes, namely pigeon pea (Cajanus cajan) and lima bean (Phaseolus lunatus), as alternative protein sources. Methods. Experimental diets (pigeon pea-based and lima bean-based) and control diet (casein-based) were formulated and standardized using proximate analysis to have 10% protein basal diet based on the Association of Official Analytical Chemists (AOAC) guidelines. Diets were given to corresponding groups (2 experimental, 1 control) using 30 albino Sprague-Dawley weanling rats aged 21 days old, over a 28-day growth assay. Results. Results showed that the mean final weight and weight gain were significantly higher in rats fed with caseinbased diet (p<0.05 for both parameters) compared to those fed with pigeon pea-based diet and lima bean-based diet. The two experimental groups showed no significant difference between their mean final weights and weight changes (p=1, p>0.05). The PER of the casein (3.37 ± 2.71) is higher than that of the pigeon pea (1.87) and lima bean (1.32). These results can be attributed to the presence of anti-nutritional factors (ANFs) in the seeds of pigeon pea and lima bean. Reduced protein intake (due to bitter taste), toxicity, and interference with protein digestibility (through interaction with digestive enzymes) imparted by these ANFs can explain the weight loss among the experimental groups and consequently the low PER. Heat treatment done in the study were not adequate to remove the ANFs. Conclusion. The study shows that pigeon pea and lima bean as protein sources alone are not as adequate and efficient in providing necessary protein requirements for weanling rats. Proper processing and treatment should be done to remove inhibitors of protein digestibility and quality.

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