scholarly journals First Report of Phytophthora capsici Infecting Lima Bean (Phaseolus lunatus) in the Mid-Atlantic Region

Plant Disease ◽  
2002 ◽  
Vol 86 (9) ◽  
pp. 1049-1049 ◽  
Author(s):  
C. R. Davidson ◽  
R. B. Carroll ◽  
T. A. Evans ◽  
R. P. Mulrooney ◽  
S. H. Kim
Keyword(s):  
Phytophthora Capsici ◽  
Petri Dish ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Pathogenicity Test ◽  
Atlantic Region ◽  
Kent County ◽  
Original Field ◽  
Lima Beans ◽  
Stem Lesions

Lima beans are an important crop in Delaware and the Mid-Atlantic Region. In the summer of 2000, five commercial cultivars (3–28, 184–85, C-elite Sel, Butter Bean, and Jackson Wonder) of lima bean in Delaware, Maryland, and New Jersey were observed with white, appressed mycelia on infected pods that appeared distinctly different from signs of downy mildew infection caused by Phytophthora phaseoli. Isolations were made by placing diseased pods between layers of rye media (1). A fungus that produced white mycelia with sporangia was consistently isolated. All Phytophthora isolates from the infected pods were heterothallic, grew at 35°C, had as much as 100 μm long pedicles on varying shapes of caducous sporangia with tapering base and >2 papillae, and were identified as P. capsici (2). Initially, three surface-disinfected pods from cv. Early Thorogreen plants grown in the greenhouse were floated on 20 ml of sterile water in a petri dish, and each was inoculated with a disk of P. capsici. This was repeated for nine isolates obtained from lima bean. After incubation for 7 days at room temperature, all 27 pods were infected, and P. capsici was reisolated from all the pods. A pathogenicity test was performed on the same cultivars from which the original field isolates were collected. Three seedlings and two plants with mature pods were inoculated with a sporangial suspension of each of the nine isolates and placed in a dew chamber for 5 days at 20 to 25°C and 100% relative humidity. White mycelial growth was observed on seedlings and mature pods. One inoculated plant developed brown-to-black stem lesions with white mycelia. All pods on the mature plants showed appressed, white mycelia identical to that observed in the commercial lima bean fields. P. capsici was consistently reisolated from all inoculated plants. In 2000, most infected pods in infested fields were observed low in the plant canopy or touching the soil. However, in 2001, infected pods were mostly in the lower and mid-portion of the plants observed in baby lima bean fields in Kent County, DE. References: (1) C. E. Caten and J. L. Jinks. Can. J. Bot. 46:329, 1967. (2) D. C. Erwin and O. K. Ribeiro. Phytophthora capsici. Page 264 in: Phytophthora Diseases Worldwide. The American Phytopathological Society, St Paul, MN, 1996.

scholarly journals First Report of Mefenoxam-Resistant Isolates of Phytophthora capsici from Lima Bean Pods in the Mid-Atlantic Region

Plant Disease ◽  
2008 ◽  
Vol 92 (4) ◽  
pp. 656-656 ◽  
Author(s):  
J. F. Davey ◽  
N. F. Gregory ◽  
R. P. Mulrooney ◽  
T. A. Evans ◽  
R. B. Carroll
Keyword(s):  
Foliar Application ◽  
Phytophthora Capsici ◽  
Crop Protection ◽  
Lima Bean ◽  
Fruit Rot ◽  
Resistant Isolate ◽  
Phaseolus Lunatus ◽  
Atlantic Region ◽  
History Of ◽  
Lima Beans

Phytophthora capsici Leonian, the causal agent of lima bean pod rot, was first identified as a pathogen of lima bean in 2002 (1) and poses a new threat to lima bean (Phaseolus lunatus L.) production in the Mid-Atlantic Region. The phenylamide fungicide mefenoxam (Ridomil Gold; Syngenta Crop Protection) is widely used in the region for controlling foliar and soilborne diseases caused by Oomycetes. Isolates of P. capsici were collected from lima bean pods from production fields in Delaware, Maryland, and New Jersey from 1998 to 2004. These isolates originated from survey samples of lima bean fields for another pathogen, P. phaseoli, in 1999 and 2000 and diagnostic samples were submitted to the Plant Disease Clinic. Isolates were from lima bean, except for one from pepper (basal stem). Identification was made on the basis of morphometric characteristics. No known sensitive or insensitive isolates were included in the evaluation. Single zoospore cultures were evaluated for mefenoxam sensitivity on V8 agar plates amended with 100 ppm of mefenoxam, a previously tested concentration (2). Seven-millimeter-diameter agar plugs of each isolate were cut from the edge of actively expanding cultures of P. capsici with a cork borer and transferred to three V8 agar plates amended with mefenoxam and three unamended V8 plates. The plates were arranged in a completely randomized design and incubated at 25°C in the dark for 3 days. After incubation, colony growth was measured in millimeters and averaged for the three replicate plates of each isolate and percent growth relative to the unamended control was calculated. Mefenoxam sensitivity was assigned according to methods of Lamour et al. (2). The experiment was repeated once, and also run with a treatment of 200 ppm of mefenoxam. Of sixteen isolates screened, nine were rated as sensitive, four were intermediately resistant, and three were resistant. There was no difference between the 100 and 200 ppm results, except for a slight increase in sensitivity for one isolate. A subsequent experiment tested five isolates at concentrations of 1, 10, 100, and 1,000 ppm. Results were consistent with previous tests, with resistant isolates exhibiting some growth at the highest concentration of mefenoxam. One resistant isolate was from a field in Delaware previously cropped to slicing cucumbers with a history of mefenoxam applications. The second was from Caroline County, MD, which is heavily cropped to pickling cucumbers and likely to have been exposed to mefanoxam applications for the control of fruit rot; the origin of the third insensitive isolate from lima bean is unknown. Mefanoxam usage on lima bean is usually limited to one foliar application of mefenoxam+copper hydroxide to control downy mildew in the fall crop in wet seasons. This study indicates that mefenoxam resistance is present in populations of P. capsici in lima bean fields in the Mid-Atlantic Region, presumably as a result of mefenoxam applications to other vegetable crops, principally cucurbits, which are planted in rotation with lima beans or from nearby cucurbit fields. Implementing strategies to minimize fungicide resistance in other vegetables is important to slow resistance development associated with this emerging pathogen on lima beans. Lima bean pod rot continues to be seen sporadically each year in fields with a history of P. capsici and abundant rainfall or excessive irrigation. References: (1) C. R. Davidson et al. Plant Dis. 86:1049, 2002. (2) K. H. Lamour et al. Phytopathology 90:396, 2000.

Managing Pod Rot of Lima Bean Caused by Phytophthora capsici with Fungicides in the Mid-Atlantic Region

2016 ◽  
Vol 17 (2) ◽  
pp. 130-132 ◽  
Author(s):  
Andrew A. Kness ◽  
Gordon Johnson ◽  
Kathryne L. Everts ◽  
Thomas A. Evans ◽  
Nicole M. Donofrio ◽  
...  
Keyword(s):  
Disease Incidence ◽  
Phytophthora Capsici ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
New Disease ◽  
Atlantic Region ◽  
Lima Beans

The baby lima bean (Phaseolus lunatus) is one of the most economically important crops for Delaware. Pod rot of lima bean caused by Phytophthora capsici is a relatively new disease that threatens production and profitability of lima beans grown in Delaware and the mid-Atlantic region. Insensitivity to the fungicide mefenoxam has made managing the disease challenging in recent years. In 2014, 12 fungicides in two trials were tested for their efficacy against P. capsici in lima bean. Results indicated that all fungicide treatments significantly reduced disease incidence compared to the control, with oxathiapiprolin achieving the best control in Trial 1 and fluazinam and mefenoxam achieving the best control in Trial 2. Fungicide treated plots also yielded significantly greater than controls in Trial 2. These results indicate that there are additional fungicides on the market with efficacy towards managing pod rot of lima bean and warrant further investigation for possible new labels. Accepted for publication 16 May 2016. Published 9 June 2016.

scholarly journals Total phenolics, flavonoids, tannins and antioxidant activity of lima beans conserved in a Brazilian Genebank

2014 ◽  
Vol 45 (2) ◽  
pp. 335-341 ◽  
Author(s):  
Tânia da Silveira Agostini-Costa ◽  
Ana Flávia Pádua Teodoro ◽  
Rosa de Belem das Neves Alves ◽  
Leandro Ribeiro Braga ◽  
Ieler Ferreira Ribeiro ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Abiotic Factors ◽  
Condensed Tannin ◽  
Germplasm Collection ◽  
Lima Bean ◽  
Ex Situ ◽  
Phaseolus Lunatus ◽  
Beneficial Effects ◽  
Lima Beans

The objective of this study was to characterize for the first time polyphenols and DPPH (2-diphenyl-1-picryhydrazyl radical) antioxidant activity in commonly cultivated accessions of Phaseolus lunatus from an ex situ germplasm collection maintained by Embrapa, in Brazil. Furthermore, the study aimed to detect changes in total polyphenols, total flavonoids and condensed tannin for the same accessions after regeneration in a greenhouse. The results showed the diversity of the lima bean collection for phenolic compounds, which were strongly correlated with antioxidant activity. Lima beans accessions with the highest polyphenols and antioxidant activity were those with colored seeds. Conservation through cold storage of P. lunatus seeds in a cold chamber in the germplasm collection did not necessarily affect phenolic compounds. Variations observed in values after regeneration seeds may be mainly results of biotic and abiotic factors, including not only cultivar, but also environmental conditions. This study suggests that polyphenols in the lima beans present antioxidant activity, with possible beneficial effects for human health. It was expected that the potential of this tasty legume can be also used as a functional food crop and/or as a new ingredient in gastronomy.

scholarly journals Lima Bean Downy Mildew Epiphytotics Caused by New Physiological Races of Phytophthora phaseoli

Plant Disease ◽  
2008 ◽  
Vol 92 (5) ◽  
pp. 670-674 ◽  
Author(s):  
C. R. Davidson ◽  
T. A. Evans ◽  
R. P. Mulrooney ◽  
N. F. Gregory ◽  
R. B. Carroll ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Fragment Length Polymorphism ◽  
Lima Bean ◽  
The United States ◽  
Phaseolus Lunatus ◽  
Atlantic Region ◽  
Resistant Cultivars ◽  
Physiological Races ◽  
Phytophthora Spp ◽  
Physiological Race

Before 1995, race D of Phytophthora phaseoli, the causal agent of downy mildew on lima bean (Phaseolus lunatus), was the prevalent physiological race in the mid-Atlantic region of the United States. Since 1995, however, new physiological races of P. phaseoli have been responsible for downy mildew outbreaks in previously resistant cultivars in this region. Cultivar differential testing of 180 isolates of P. phaseoli collected between 1994 and 2005 from Delaware and the eastern shore of Maryland has confirmed the presence of two new physiological races. The detection of race E in 1995 and race F only 5 years later in 2000, plus the lack of resistant cultivars to manage the epiphytotics in lima bean, have led to millions of dollars of crop losses. Intra- and interspecific genetic variation of Phytophthora spp. and isolates were assessed using amplified fragment length polymorphism DNA fingerprinting. Primer groups EcoRI+AG and MseI+C distinguished P. phaseoli and P. capsici from P. infestans but did not distinguish among different races of P. phaseoli.

Assessment of Wood Ash Application on Yield Advantage Indices of Maize and Lima Beans in an Intercrop

2019 ◽  
pp. 1-11 ◽  
Author(s):  
R. O. Ajala ◽  
M. A. Awodun ◽  
A. J. Adeyemo ◽  
B. F. Dada
Keyword(s):  
Zea Mays L ◽  
Block Design ◽  
Lima Bean ◽  
Wood Ash ◽  
Phaseolus Lunatus ◽  
Land Equivalent Ratio ◽  
University Of Technology ◽  
Randomized Complete Block Design ◽  
Lima Beans ◽  
Competition Indices

Alternate planting combinations of maize (Zea mays L.) with lima bean (Phaseolus lunatus L.) using wood ash as soil amendments were compared with the sole planting of each crop during the late 2014  and early 2015 planting seasons at the Teaching and Research Farm of the Federal University of Technology, Akure. The experiment in each season adopted three patterns of intercropping using ash as a soil amendment and laid out in a Randomized Complete Block Design (RCBD) with three replications. The experiment comprised 10 treatments: Sole planting of maize amended, sole planting of maize unamended, sole planting of lima beans amended and sole planting of lima beans unamended. Others were; 75:25 maize-lima beans amended, 75:25 maize-lima beans unamended, 50:50 maize-lima beans amended, 50:50 maize-lima beans unamended, 25:75 maize-lima beans amended and 25:75 maize-lima beans unamended. Wood ash was applied two weeks after planting at the rate of 2.4 kg (4 tons per hectare) to each plot. The combined yield advantage in terms of land equivalent ratio (LER) indices was greatest (1.95) in the case of 3 rows of maize and 1row of lima beans intercropping arrangements. Competition indices (CR) for all crops in all intercropping arrangements were more than 0.1 indicating that both crops were equally competitive.  However, crop aggressivity (A) showed that maize was more dominant than lima beans due to plant population. Costs and returns analysis revealed that maize and lima beans intercropping at all proportions were more profitable than their corresponding monocrops.

scholarly journals The Extraction and Partial Characterization of Lunatin from Lima Bean (Phaseolus lunatus L.)

2021 ◽  
Vol 2 (2) ◽  
pp. 7-12
Author(s):  
Ahmad Nafi' ◽  
Maria Belgis ◽  
Aisyah Fridannisa
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Lima Bean ◽  
Phaseolus Lunatus ◽  
Extraction Temperature ◽  
Temperature Variations ◽  
Bean Flour ◽  
Legume Flour ◽  
Lima Beans

Lima Bean (Phaseolus lunatus L.) contains a lectin called lunatin which is an antinutrient with antioxidant, antifungal, and antiproliferative properties. The purpose of this study was to determine the effect of different processing of Lima bean seeds, namely crushed dry seeds, Lima bean flour, and Modified Legume Flour (MOLEF). In addition, it delved into the differences resulting from different extraction temperatures (27ºC, 40ºC, and 50ºC) on the lunatin extract obtained. The results show that the total protein in dry seeds, flour and MOLEF Lima beans are 23.18%, 24.20% and 17.12%, respectively. The molecular weight of lunatin detected is 30.25 kDa; The highest antioxidant activity, marked at 83.58% , is obtained by lunatin extract from samples of crushed dried Lima beans, with an extraction temperature of 50ºC, while the lowest activity, 45.97%, is indicated by lunatin extract from MOLEF samples of Lima beans, with extraction temperature of 40ºC. Temperature variations in general do not affect the protein bands detectable. Likewise, the antioxidant activity also does not show antifungal activity against Aspergillus niger.

scholarly journals (254) Genetic Improvement of the Fordhook Lima Bean (Phaseolus lunatus) for Vegetable Processing by Single-seed Decent

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1072D-1072
Author(s):  
Mathew Wilson ◽  
Jeffrey C. Wong
Keyword(s):  
Lima Bean ◽  
Descent Method ◽  
Chemical Mutagenesis ◽  
Phaseolus Lunatus ◽  
Temperate Climate ◽  
Single Seed ◽  
Heat Tolerant ◽  
Method Of Production ◽  
Lima Beans ◽  
Current Lines

The Fordhook lima bean is a large-seeded lima bean grown in California for use by vegetable processors as an ingredient in frozen mixed vegetable packs. California represents one of the largest production regions for this bean because of the temperate climate. Unfortunately, as the population of California grows, the land available for producing this relatively low-valued crop continues to diminish. To overcome the loss of productive lands, we have initiated two projects, both focused on an increase in productivity. The first project is the improvement of current lines used by one processor. Due to the method of production of the seed bean, as well as the age of current lines in use, plant vigor and uniformity within the fordhook lima bean population has decreased over time. By using a modified single-seed descent method within the population, a certain level of improvement of production lines should be realized. Our second objective is in the development of a more heat-tolerant line, which could be grown in the inner valleys of California, where more land is available. Because of the limited availability of heat-tolerant Fordhook lima beans, we are currently investigating the possibility of using chemical mutagenesis to produce a more heat-tolerant variety.

scholarly journals Silicon as resistance inducer in to control black aphid Aphis craccivora Koch, 1854 in Phaseolus lunatus lima beans

2019 ◽  
Vol 86 ◽  
Author(s):  
Gilson Lages Fortes Portela ◽  
Paulo Roberto Ramalho Silva ◽  
José Edmir Girão Filho ◽  
Luiz Evaldo de Moura Pádua ◽  
Luiz Carlos de Melo Júnior
Keyword(s):  
Lignin Content ◽  
Lima Bean ◽  
Reproductive Period ◽  
Aphis Craccivora ◽  
Phaseolus Lunatus ◽  
Biological Variables ◽  
Aphis Craccivora Koch ◽  
Biological Aspects ◽  
Lima Beans ◽  
And Control

ABSTRACT In Brazil, there are few records of insects associated with the cultivation of lima beans; among them, there is the black aphid Aphis craccivora Koch, 1854. The objective of this study was to evaluate the effects of silicon application on the resistance induction of lima bean plants, Phaseolus lunatus, to the black aphid A. craccivora. The experiment was conducted in the Entomology Laboratory of the Phytosanitary Sector of Centro de Ciências Agrárias, Universidade Federal do Piauí (UFPI), Brazil. The effects of the following treatments on biological aspects of the insect were evaluated: silicon applied to soil; silicon applied to soil + leaf; silicon applied to leaf; and control, without silicon application. The following biological variables were evaluated: generation period, reproductive period, and the fertility and daily average of produced nymphs per female. Plant silicon and lignin content were also evaluated. A 1% solution of silicic acid (2.0 g of product diluted in 200 mL of water) was applied around the plant stem (on soil), 15 days after emergence. Leaf application was performed with a 1-L spray, 5 days after the soil application. The non-preference of A. craccivora on lima beans was also evaluated. The evaluations were performed after 48 and 72 hours of infestation by counting nymphs and adults at each leaf section. Silicon application reduces nymph production, thereby interfering in the biological aspects of A. craccivora. Therefore, it can be used in cowpea pest management programs.

scholarly journals Proximate composition, phytochemical screening and some antinutritional factors of three accessions of lima beans (Phaseolus lunatus ) an underutilized legume

2020 ◽  
Vol 45 (3) ◽  
Author(s):  
K. O. Soetan ◽  
T. N. Atanda
Keyword(s):  
Proximate Composition ◽  
Crude Protein ◽  
Lima Bean ◽  
Proximate Analysis ◽  
Antinutritional Factors ◽  
Phaseolus Lunatus ◽  
International Institute ◽  
Phytochemical Screening ◽  
Crude Fibre ◽  
Lima Beans

Lima bean (LB) (Phaseolus lunatus) is an underutilized legumein Nigeria and West Africa despite its numerous nutritional benefits. This study evaluated the proximate composition, phytochemical screening and antinutritional factors (ANFs) of three accessions of Lima beans; LB 001, LB 011 and LB 015 from the International Institute of Tropical Agriculture (IITA), Ibadan, Oyo State. All analyses were done using standard protocols. Statistical analysis was done using descriptive statistics. Result of proximate analysis revealed that LB 011 recorded the highest value for crude protein (25.70 0.06%), crude fat (3.10 0.05%) and crude fibre (3.20 0.06%) and moisture content (9.3 0.16%), LB 015 recorded the highest value for ash (3.90 0.04%), nitrogen free extract (60.35 0.20%) while LB001 gave the highest value for drymatter (92.30 2.50%).Phytochemical screening revealed presence of saponin, flavonoid and alkaloid in the three accessions of LB while anthraquinone and cardiac glycoside were absent in all the LB accessions. Results of ANFs showed that LB 001 recorde the highest value for alkaloid (56.67±5.78mg/g), LB 015 gave the highest value for tannin (93.33±2.89 mg/g) and saponin (61.67±2.89 mg/g) while LB 011 had the highest oxalate content (30±5.00mg/g). The study concluded that the three accessions of lima beans have high levels of crude protein, crude fat and ash and phytochemicals, which compared favourably with that of cowpea (Vigna unguiculata), a conventional legume. However, the ANFs present in LBneed adequate processing for their reduction and optimal utilization as a protein source for human and animals.

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