scholarly journals Effect of Novel Penicillium verruculosum Enzyme Preparations on the Saccharification of Acid- and Alkali-Pretreated Agro-Industrial Residues

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1348
Author(s):  
Susan G. Karp ◽  
Dmitrii O. Osipov ◽  
Margarita V. Semenova ◽  
Alexandra M. Rozhkova ◽  
Ivan N. Zorov ◽  
...  

This study aimed at evaluating different enzyme combinations in the saccharification of sugarcane bagasse (SCB), soybean husks (SBH) and oil palm empty fruit bunches (EFB) submitted to mild acid and alkaline pretreatments. Enzyme pools were represented by B1 host (crude cellulase/xylanase complexes of Penicillium verruculosum); B1-XylA (Penicillium canescens xylanase A expressed in P. verruculosum B1 host strain); and F10 (Aspergillus niger β-glucosidase expressed in B1 host strain). Enzyme loading was 10 mg protein/g dry substrate and 40 U/g of β-glucosidase (F10) activity. SCB was efficiently hydrolyzed by B1 host after alkaline pretreatment, yielding glucose and reducing sugars at 71 g/L or 65 g/100 g of dry pretreated substrate and 91 g/L or 83 g/100 g, respectively. B1 host performed better also for EFB, regardless of the pretreatment method, but yields were lower (glucose 27–30 g/L, 25–27 g/100 g; reducing sugars 37–42 g/L, 34–38 g/100 g). SBH was efficiently saccharified by the combination of B1 host and B1-XylA, yielding similar concentrations of reducing sugars for both pretreatments (92–96 g/L, 84–87 g/100 g); glucose recovery, however, was higher with alkaline pretreatment (81 g/L, 74 g/100 g). Glucose and reducing sugar yields from initial substrate mass were 42% and 54% for SCB, 36% and 42–47% for SBH and 16–18% and 21–26% for EFB, respectively.

2019 ◽  
Vol 35 (5) ◽  
pp. 51-57 ◽  
Author(s):  
V.Yu. Kislitsin ◽  
I.N. Zorov ◽  
A.P. Sinitsyn ◽  
A.M. Rozhkova

The protein profiles of the culture liquids of recombinant Penicillium verruculosum strains that secreted an Aspergillus niger β-glucosidase (BGL series) or a Penicillium canescens xylanase A (XYLA series) have been previously analyzed. It was shown that the producers were divided into two groups according to the target protein content: 75-85% (1) and no more than 15% (2). It was established in this work that the copy number of the target gene bgll in the recombinant strains correlated with the β-glucosidase content in the BGL-F10 and BGL-F12 enzyme preparations. However, this proportion was violated in the direction of decreasing the xylanase A relative amount: the target enzyme content of 50% and 17% in the XylA3 and XylA4 preparations corresponded to 20-22 and 6 copies, respectively, of the xylA gene. We believe that this effect is a result of titration of positive transcription factors in the P. verruculosum XylA3 strain containing 20 to 22 copies of the cbhl gene promoter. Penicillium verruculosum, real-time PCR, gene copy number This work was partially supported by the Russian Foundation for Bsic Research (Project number: 18-29-07070).


Energies ◽  
2019 ◽  
Vol 12 (16) ◽  
pp. 3129 ◽  
Author(s):  
Kamila Przybysz ◽  
Edyta Małachowska ◽  
Danuta Martyniak ◽  
Piotr Boruszewski ◽  
Halina Kalinowska ◽  
...  

This study showed that kraft cellulosic pulps from Miscanthus giganetus JM Greef and Deuter ex Hodk. and Renvoize, sweet sorghum and 5 other fast growing grasses may be easily enzymatically converted to glucose-rich sugar feedstocks. The scientific goal of the paper was to assess and compare the potential yield of hydrolysis and verify whether these grasses may be a source of sugars for fermentation processes. Kraft pulping was used as a pretreatment method and hydrolysis of the pulps was conducted using a commercial multienzyme preparation containing cellulases and xylanases at initial substrate concentrations of 0.476, 3.88 and 7.46% w/v, and 3 different enzyme loadings. Results showed that tall wheatgrass, striped tuber oat grass, tall fescue and smooth bromegrass may be efficiently converted to sugar feedstocks for biotechnology application, but that the simple reducing sugars yield is lower than for wood, due to lower cellulose content.


Author(s):  
Sasikarn Nuchdang ◽  
Vipa Thongtus ◽  
Maneerat Khemkhao ◽  
Suchata Kirdponpattara ◽  
Elvin J. Moore ◽  
...  

1946 ◽  
Vol 24c (2) ◽  
pp. 47-53 ◽  
Author(s):  
Paul R. Gorham

The soluble carbohydrates were extracted, by means of hot water, from dried ground roots of Taraxacum kok-saghyz Rod. that had been extracted previously with acetone and benzene. A cleared portion of the extract served for the determination of hexose and, after invertase hydrolysis, sucrose. Another portion was subjected to mild acid hydrolysis before clearing, and served for the determination of total reducing value, whence fructosans, as inulin, were calculated. Separation of the free reducing sugars and sucrose from the fructosans by ethanol extraction proved unsatisfactory. Analyses of 171 one-year old roots from six crosses gave the following average values expressed as per cent dry weight: hexose 1.6, sucrose 4.7, and inulin 41. The analytical data suggest the possibility of selecting and breeding for strains of kok-saghyz capable of high carbohydrate production.


Energies ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5891
Author(s):  
Rocío Maceiras ◽  
Víctor Alfonsín ◽  
Luis Seguí ◽  
Juan F. González

Biomass pretreatment has an important role in the production of cellulosic bioethanol. In this study, the effectiveness of microwave assisted alkaline pretreatment of algae waste was analysed. After pretreatment, the product was hydrolysed using sulphuric acid. The effects of microwave power, irradiating time, solid–liquid ratio and NaOH concentration were examined. Under the best conditions, the fermentable sugars were converted to cellulosic bioethanol using Saccharomyces Cerevisiae with a bioethanol yield of 1.93 ± 0.01 g/g and a fermentation efficiency of 40.4%. The reducing sugars concentration was 30% higher than that obtained from conventional hydrolysis without pretreatment. The obtained results suggest that microwave assisted alkaline pretreatment is effective in improving the production of cellulosic bioethanol of algae waste compared to that without microwave effect. Considering energy consumption, low microwave power and short microwave irradiation time are favourable for this pretreatment.


2015 ◽  
Vol 80 (4) ◽  
pp. 473-482 ◽  
Author(s):  
D. A. Merzlov ◽  
I. N. Zorov ◽  
G. S. Dotsenko ◽  
Yu. A. Denisenko ◽  
A. M. Rozhkova ◽  
...  

2020 ◽  
Vol 56 (8) ◽  
pp. 875-880 ◽  
Author(s):  
A. P. Sinitsyn ◽  
O. G. Korotkova ◽  
E. A. Rubtsova ◽  
O. A. Sinitsyna ◽  
E. G. Kondrat’eva ◽  
...  

2014 ◽  
Vol 6 (4) ◽  
pp. 348-354 ◽  
Author(s):  
E. V. Novozhilov ◽  
A. S. Aksenov ◽  
M. L. Demidov ◽  
D. G. Chukhchin ◽  
G. S. Dotsenko ◽  
...  

Cellulose ◽  
2016 ◽  
Vol 23 (3) ◽  
pp. 1491-1520 ◽  
Author(s):  
Yu-Loong Loow ◽  
Ta Yeong Wu ◽  
Jamaliah Md. Jahim ◽  
Abdul Wahab Mohammad ◽  
Wen Hui Teoh

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