scholarly journals Soybean Response to Seed Coating with Chitosan + Alginate/PEG and/or Inoculation

Agronomy ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1737
Author(s):  
Wacław Jarecki
Keyword(s):  
Research Network ◽  
Good Effect ◽  
Soybean Seeds ◽  
Main Role ◽  
Seed Coating ◽  
Agricultural Practice ◽  
The Novel ◽  
Coating Agent ◽  
Commercial Inoculant ◽  
Inoculation Procedure

Inoculated or coated soybean seeds are often sown in agricultural practice. These treatments play a different role depending on the chemical composition of the preparation. The aim of the field experiment was to demonstrate the effectiveness of the developed coating (chitosan + alginate/PEG) and commercial inoculant (HiStick® Soy) applied alone or in combination to soybean seeds. Uncoated (control) seeds were sown for comparison. The research was carried out in 2018–2020 using the cultivar ‘Mavka’. The experiment was located in Makowisko, Podkarpackie Province, Poland. Coating composition was developed in a laboratory belonging to the Łukasiewicz Research Network—Institute of Biopolymers and Chemical Fibers in Łódź, Poland. The main role of the coating is to protect soybean seeds from low temperatures. HiStick® Soy inoculant contains Bradyrhizobium japonicum bacteria which increase nodulation on the roots. The conducted research demonstrated that sowing only coated seeds was not very effective, because the suitable number of nodules had not developed on soybean roots. The application of the inoculant alone positively affected the assessed traits compared to control, however, plant population was lower than expected. The highest seed yield was obtained after sowing coated seeds in combination with the inoculant (4.32 t·ha−1) and only inoculated seeds (4.23 t·ha−1) compared to control (3.64 t·ha−1). The test of the novel seed-coating agent showed that it had an good effect and efficacy, but only in combination with the inoculation procedure.

scholarly journals Development and Application of Seed Coating Agent for the Control of Major Soil-Borne Diseases Infecting Wheat

Agronomy ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 413 ◽  
Author(s):  
Ren ◽  
Chen ◽  
Ye ◽  
Su ◽  
Xiao ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Seed Coating ◽  
Practical Application ◽  
Active Constituent ◽  
Coating Agent ◽  
Grinding Method ◽  
Fungal Inhibition ◽  
Sharp Eyespot ◽  
Coating Agents ◽  
Seed Germination Test

In order to reduce the usage amount of pesticide fertilizers and protect the natural environment, seed coating agents are receiving increased wide concern. In this study, the active constituent (pesticide) and inactive components (surfactants and film former) of the seed coating agents were screened and optimized by the wet sand processing superfine grinding method. The fungal inhibition test of pesticides showed that thifluzamide, fludioxonil, pyraclostrobin, and difenoconazole have an obvious fungal inhibitory effect on wheat sharp eyespot, take-all, and root rot. LAE-9 and polyacrylamide + carboxymethyl cellulose (CMC) is recommended for the safe surfactant and film former, respectively, based on the seed germination test. Moreover, 6% difenoconazole · fludioxonil flowable concentrate for seed coating (FSC) stimulates the seedling growth of wheat, advances the growth of root, and improves biomass in the field trial, meanwhile, the control efficiency reached above 80%. Thus, we suggested it can be used as an effective seed coating agent for the control of soil-borne diseases in wheat. The seed coating agent has the characteristics of disease prevention, increasing crop yield, and safety of environment, which is of significance in practical application.

scholarly journals Influence of Oilseed Rape Seed Treatment with Imidacloprid on Survival, Feeding Behavior, and Detoxifying Enzymes of Mustard Aphid, Lipaphis erysimi

Insects ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 144
Author(s):  
Fang Huang ◽  
Zhongping Hao ◽  
Fengming Yan
Keyword(s):  
Feeding Behavior ◽  
Oilseed Rape ◽  
Lipaphis Erysimi ◽  
Seed Coating ◽  
Detoxifying Enzymes ◽  
Feeding Behaviors ◽  
Mustard Aphid ◽  
Coating Agent ◽  
Aphid Feeding ◽  
Rape Seedlings

Imidacloprid application, as a seed coating agent on oilseed rape, is recommended to control mustard aphid, Lipaphis erysimi (Kaltenbach) (Hemiptera: Aphididae). In this study, responses of L. erysimi were investigated, including survival, feeding behavior, and detoxifying enzymes, on the oilseed rape seedlings grown from seeds coated with imidacloprid at rates of 6, 12, or 18 g active ingredient (a.i.)/kg seed. The results showed that the aphids’ survival rate, together with that of the progeny of the survivors, on the seed-treated seedlings significantly decreased. This indicates that the aphid population in fields can be suppressed effectively. The electrical penetration graph (EPG) technique was used to record aphid feeding behaviors on two-, four-, and six-leaf stages of oilseed rape seedlings that had been seed-coated with imidacloprid, and individual responses were revealed during the aphid feeding behavior. On the plants at the two-leaf stage, aphid feeding behaviors were influenced, showing decreased frequency of stylet penetration into the leaf (probe) or into the mesophyll cells (potential drops, pds for short), and shortened duration of stylet event in the leaf (probe) or in the phloem. On the plants at the four- and six-leaf stages, these impacts of imidacloprid were weakened; however, the saliva secretion duration in phloem was shortened to less than 5 min in all imidacloprid treatments. The activity of mixed-function oxidase in aphids maintained on the treated seedlings with imidacloprid was elevated. In conclusion, imidacloprid could be used as a seed coating agent for aphid control, but chemical resistance in aphids should not be ignored.

scholarly journals RamA, a transcriptional regulator conferring florfenicol resistance in Leclercia adecarboxylata R25

2020 ◽  
Vol 65 (6) ◽  
pp. 1051-1060
Author(s):  
Cong Cheng ◽  
Yuanyuan Ying ◽  
Danying Zhou ◽  
Licheng Zhu ◽  
Junwan Lu ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Efflux Pump ◽  
Gene Knockout ◽  
Regulatory Gene ◽  
Resistance Mechanism ◽  
Agricultural Practice ◽  
The Novel ◽  
Inappropriate Use ◽  
Rnd Efflux Pump ◽  
High Level

AbstractDue to the inappropriate use of florfenicol in agricultural practice, florfenicol resistance has become increasingly serious. In this work, we studied the novel florfenicol resistance mechanism of an animal-derived Leclercia adecarboxylata strain R25 with high-level florfenicol resistance. A random genomic DNA library was constructed to screen the novel florfenicol resistance gene. Gene cloning, gene knockout, and complementation combined with the minimum inhibitory concentration (MIC) detection were conducted to determine the function of the resistance-related gene. Sequencing and bioinformatics methods were applied to analyze the structure of the resistance gene-related sequences. Finally, we obtained a regulatory gene of an RND (resistance-nodulation-cell division) system, ramA, that confers resistance to florfenicol and other antibiotics. The ramA-deleted variant (LA-R25ΔramA) decreased the level of resistance against florfenicol and several other antibiotics, while a ramA-complemented strain (pUCP24-prom-ramA/LA-R25ΔramA) restored the drug resistance. The whole-genome sequencing revealed that there were five RND efflux pump genes (mdtABC, acrAB, acrD, acrEF, and acrAB-like) encoded over the chromosome, and ramA located upstream of the acrAB-like genes. The results of this work suggest that ramA confers resistance to florfenicol and other structurally unrelated antibiotics, presumably by regulating the RND efflux pump genes in L. adecarboxylata R25.

scholarly journals Seed Coating with Arbuscular Mycorrhizal Fungi for Improved Field Production of Chickpea

Agronomy ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 471 ◽  
Author(s):  
Inês Rocha ◽  
Isabel Duarte ◽  
Ying Ma ◽  
Pablo Souza-Alonso ◽  
Aleš Látr ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal ◽  
Field Conditions ◽  
Growth And Yield ◽  
Seed Coating ◽  
Agricultural Practice ◽  
Agricultural Crops ◽  
Field Inoculation ◽  
Inoculation Methods ◽  
Fungal Isolates ◽  
The Impact

Although arbuscular mycorrhizal (AM) fungi are known to promote growth and yield of agricultural crops, inoculation methods for effective scaling up from greenhouse to the field are still underexplored. The application of single or mixed beneficial AM fungal isolates is hindered by the lack of experimental reproducibility of findings at different scales and the cost-effectivity of inoculation methods. Seed coating has been considered a feasible delivery system of AM fungal inocula for agricultural crops. In this study, the impact of single and multiple AM fungal isolates applied via seed coating on chickpea productivity was evaluated under greenhouse and field conditions. Overall, plants inoculated with multiple AM fungal isolates had better performance than those inoculated with single AM isolate under greenhouse and field conditions. While plants in greenhouse displayed higher shoot dry weight (14%) and seed individual weight (21%), in field, inoculation with multiple AM isolates increased pod (160%), and seed (148%) numbers, and grain yield (140%). Under field conditions, mycorrhizal root colonization was significantly higher in chickpea plants inoculated with multiple AM fungal isolates compared to other treatments. These findings highlight the potential of field-inoculation with multiple AM fungal isolates via seed coating as a sustainable agricultural practice for chickpea production.

scholarly journals Application of Bioactive Coatings Based on Chitosan for Soybean Seed Protection

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Defang Zeng ◽  
Xinrong Luo ◽  
Renjie Tu
Keyword(s):  
Soybean Seed ◽  
Soybean Aphid ◽  
Soybean Seeds ◽  
Bioactive Coatings ◽  
Coating Agent ◽  
Soybean Yield ◽  
Chitosan Coating ◽  
Pod Borer ◽  
Made In

Soybean seeds suffer attacks of various pests that result in a decreased yield in northeastern China. Until recently, people use pesticides such as insecticides to achieve the goal of controlling pests. Chitosan extracted from deacetylation of chitin is promising candidates as a seed-coating agent to control agrotis ypsilon, soybean pod borer, and soybean aphid effectively. An experimental study on influences of chitosan with different concentrations on pest controlling and soybean growth was made in the paper. Coating based on chitosan was used as a feeding deterrent and for enhancing the germination and quality of soybean seeds. Results indicated that all chitosan coating had a significant effect on antifeeding against pests; with the increasing concentration, antifeedant rate (AR) were increased obviously, especially when in the concentration of 5%, santifeedant rate of agrotis ypsilon, soybean pod borer, and soybean aphid reached 82.89%, 87.24%, and 80.21%, respectively. Also chitosan coating increased seed germination, plant growth, and soybean yield efficiently, especially when, in the concentration of 5%, the yield was increased by about 20% compared with CK. The application of chitosan in soybean seed coated is an appropriate option to control pests replacing high-toxicity pesticides and enhance soybean yield.

Preparation and mechanism analysis of an environment-friendly maize seed coating agent

2017 ◽  
Author(s):  
Defang Zeng ◽  
Zhao Fan ◽  
Xu Tian ◽  
Wenjin Wang ◽  
Mingchun Zhou ◽  
...  
Keyword(s):  
Seed Coating ◽  
Mechanism Analysis ◽  
Maize Seed ◽  
Environment Friendly ◽  
Coating Agent

scholarly journals 86. Access to Antimicrobial Susceptibility Testing for Novel Gram-Negative Antibiotics

2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S159-S159
Author(s):  
Yuman Lee ◽  
Juliette Kim ◽  
Nicole Bradley
Keyword(s):  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
Research Network ◽  
Novel Agents ◽  
The Novel ◽  
Gram Negative ◽  
Electronic Survey ◽  
Antimicrobial Regimen ◽  
The U.S

Abstract Background Antimicrobial susceptibility testing (AST) is critical in identifying the optimal antimicrobial regimen for individual patients with serious gram-negative infections. Limitations to AST for newly developed antibiotics include the lack of commercially available AST methods, challenges of implementation due to regulations, and delays in obtaining results. The purpose of this study was to evaluate the access to AST for cefiderocol (FDC), imipenem-relebactam (IPR), meropenem-vaborbactam (MEV), and eravacycline (ERV) in hospitals across the U.S. Methods An electronic survey was distributed to the American College of Clinical Pharmacist Infectious Diseases Practice and Research Network in May 2021. Hospital baseline demographics and current practices were collected. Results Based on 50 responses, specimens were sent to in-house microbiology labs (37, 74%), core microbiology labs (8, 16%), and 3rd party reference microbiology labs (5, 10%). AST for FDC was performed by 13 (35%) in-house labs, 4 (50%) core labs, and 1 (20%) reference lab. AST for IPR was performed by 11 (30%) in-house labs, 2 (25%) core labs, and 1 (20%) reference lab. AST for MEV was performed by 25 (68%) in-house labs, 3 (37.5%) core labs, and 1 (20%) reference lab. AST for ERV was performed by 1 (20%) in-house lab, 1 (20%) core lab, and 0 reference labs. 15 (30%) respondents were not able to get AST for any of the novel agents at their respective microbiology labs. Turn-around-times (TATs) for FDC, IPR, MEV, and ERV were ≥72 hours for 33 (66%), 35 (70%), 21 (42%), and 35 (70%) hospitals, respectively. When compared with 3rd party reference labs, in-house labs with the ability to perform AST for these novel agents had significantly shorter TATs (p< 0.05). The average number of requests for AST for these novel agents was 20 times a year with an average of 113 minutes spent per patient on the coordination of AST. Conclusion Access to AST for these novel agents varied across hospitals in the U.S. Nearly 1/3 of the respondents were not able to obtain AST for these agents at all. Long TATs exist and a great deal of time is spent per patient for coordinating AST for these novel agents. There is a crucial need for a multidisciplinary, collaborative approach to resolve the challenges in obtaining AST for newly developed antibiotics to provide patient care. Disclosures All Authors: No reported disclosures

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