scholarly journals Influence of Zinc Oxide Nanoparticles to Regulate the Antioxidants Enzymes, Some Osmolytes and Agronomic Attributes in Coriandrum sativum L. Grown under Water Stress

Agronomy ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2004
Author(s):  
Muhammad Tajammal Khan ◽  
Shakil Ahmed ◽  
Anis Ali Shah ◽  
Adnan Noor Shah ◽  
Mohsin Tanveer ◽  
...  

Climatic variations adversely affect the limited water resources of earth which leads to water stress and influences agricultural production worldwide. Therefore, a novel approach has been introduced to improve the tolerance against water stress in herbaceous nature medicinal plants such as Coriandrum sativum by the usage of nanotechnology (foliar applied nanoparticles of ZnOx) coupled with the application of water deficit irrigation. This is an alternative water saving strategy that proved to be efficient to mitigate the Coriandrum sativum tolerance against water stress regimes for sustainable yield production through the activation of antioxidant system. Thus, the phenomena of green synthesis have been deployed for the formation of Zinc oxide nanoparticles (ZnOx NPs) from the leaf extract of Camellia sinensis L. and zinc acetate dihydrate was used as precursor. Different techniques have been used for the thorough study and confirmation of ZnOx NPs such as UV-vis spectroscopy (UV-vis) X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and Elemental dispersive spectroscopy (EDS). The prepared ZnOx NPs exhibit hexagonal wurtzite crystal nature has an average size of 37 nm with high purity. These ZnOx NPs have been further studied for their role in amelioration of water stress tolerance in Coriandrum sativum in a pot experiment. Two levels of water stress regimes were employed, IR75 (moderate) and IR50 (Intense) to evaluate the behavior of plant compared to full irrigation (FI). Results showed that under water stress regimes, the 100 ppm of prepared NPs stimulate the antioxidant system by increasing the activity of catalases (CAT), super oxidases (SOD) and ascorbate peroxidase (APX) enzymes and found the maximum at IR50, while the concentration of malondialdehyde (MDA) decreased due to increase in activity of antioxidative enzymes. Furthermore, chlorophyll content and amount of proline also enhanced by the foliar application of prepared ZnOx NPs under moderate water stress (IR75). The results suggested that all the investigated agronomic attributes significantly increased, including plant biomass and economic yield (EY), compared to non-treated ZnOx NPs plants, except for the number of primary branches and LAI. Further, the 100 ppm of prepared ZnOx NPs have great potential to improve water stress tolerance in Coriandrum sativum by improving the antioxidant enzymes activity that enhance agronomic attributes for high crop productivity that require further research at transcriptomic and genomic level.

2021 ◽  
Author(s):  
Balaji GL ◽  
S. Mahesh ◽  
Boya Palajonnala Narasaiah ◽  
Sadegh Rostamnia ◽  
Harihara Padhy ◽  
...  

Abstract The present report an environmental benign route for the fabrication of Zinc Oxide nanoparticles (ZnO-NPs) using sunflower leaf aqueous extract at room temperature. This was an green method is a rapid biogenic and offers few advantages over the chemical and physical procedures, as it is an easy and fast, eco-friendly and does not involve any costly chemicals as well as hazardous chemicals, harmful solvent. The synthesized ZnO-NPs were characterized using different technique such as UV-Visible spectroscopy. The formation of ZnO-NPs was confirmed by Surface Plasmon Resonance (SRP) at 344 nm using UV-Vis spectroscopy. The leaf extract act as a source of phyto-chemicals and are the primarily responsible for the reduction and then formation of stable Zinc Oxide nanoparticles by the characteristic functional groups of extract and synthesized ZnO-NPs were identified by FTIR spectroscopy. Crystalline nature of ZnO-NPs was confirmed by powder –XRD. Size and morphology were measured by HR-TEM analysis. Stability of the nanoparticles is represented by DLS and TGA analysis. The synthesized ZnO-NPs has been found good and efficient catalyst for the synthesis of novel 1,2-dihydroquinazoline derivatives under green method via one pot reaction of 2-amino benzophenone, 1,3-diphenyl-1 H -pyrazole carbaldehydes and ammonium acetate. The synthesized compounds (4a-o) were characterized by 1 HNMR, 13 CNMR and HRMS spectra and further screened for radical scavenging activity. Among all the compounds showed good percentage of inhibition against standard ascorbic acid. The synthesized ZnO nanoparticles showed good antioxidant activity and also act as a good promising material for catalytic agent.


2001 ◽  
Vol 28 (11) ◽  
pp. 1095 ◽  
Author(s):  
Hernán R. Lascano ◽  
Gerardo E. Antonicelli ◽  
Celina M. Luna ◽  
Mariana N. Melchiorre ◽  
Leonardo D. Gómez ◽  
...  

The participation of the antioxidant system in the drought tolerance of wheat cultivars (Triticum aestivum L.) was studied under field and in vitro conditions. Under field conditions, drought tolerance was evaluated by the capacity to maintain the grain yield under drought, which was higher in cvv. Elite and La Paz than in the sensitive cvv. Oasis and Cruz Alta. Tolerant cultivars showed lower relative water content (RWC) and lower above-ground vegetative biomass than sensitive cultivars. Field assays did not show a clear correlation between water-stress tolerance and antioxidant system behaviour. However, when leaves of cvv. with contrasting drought tolerance were subjected to osmotic stress in vitro, clear differences in the antioxidant system activity and oxidative damage between cvv. were observed. In the tolerant cultivar Elite, it was possible to observe an increase in ascorbate peroxidase (APX), superoxide dismutase (SOD) and glutathione reductase (GR) activities, a higher glutathione (GSH) and ascorbate content and less oxidative damage than in the sensitive cultivar Oasis, which showed no changes or only slight decreases in the enzyme activities. These results indicate that water stress tolerance is in part associated with the antioxidant system activity, and suggest that the behaviour of the antioxidant systemin vitro assays can be used as an early selection tool.


2021 ◽  
Vol 161 ◽  
pp. 122-130
Author(s):  
Mohammad Faizan ◽  
Javaid Akhter Bhat ◽  
Chen Chen ◽  
Mohammed Nasser Alyemeni ◽  
Leonard Wijaya ◽  
...  

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 884
Author(s):  
Temoor Ahmed ◽  
Zhifeng Wu ◽  
Hubiao Jiang ◽  
Jinyan Luo ◽  
Muhammad Noman ◽  
...  

Burkholderia glumae and B. gladioli are seed-borne rice pathogens that cause bacterial panicle blight (BPB) disease, resulting in huge rice yield losses worldwide. However, the excessive use of chemical pesticides in agriculture has led to an increase in environmental toxicity. Microbe-mediated nanoparticles (NPs) have recently gained significant attention owing to their promising application in plant disease control. In the current study, we biologically synthesize zinc oxide nanoparticles (ZnONPs) from a native Bacillus cereus RNT6 strain, which was taxonomically identified using 16S rRNA gene analysis. The biosynthesis of ZnONPs in the reaction mixture was confirmed by using UV–Vis spectroscopy. Moreover, XRD, FTIR, SEM-EDS, and TEM analysis revealed the functional groups, crystalline nature, and spherical shape of ZnONPs with sizes ranging from 21 to 35 nm, respectively. Biogenic ZnONPs showed significant antibacterial activity at 50 µg mL−1 against B. glumae and B. gladioli with a 2.83 cm and 2.18 cm zone of inhibition, respectively, while cell numbers (measured by OD600) of the two pathogens in broth culture were reduced by 71.2% and 68.1%, respectively. The ultrastructure studies revealed the morphological damage in ZnONPs-treated B. glumae and B. gladioli cells as compared to the corresponding control. The results of this study revealed that ZnONPs could be considered as promising nanopesticides to control BPB disease in rice.


Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1998
Author(s):  
Norma Ruiz-Torres ◽  
Antonio Flores-Naveda ◽  
Enrique Díaz Barriga-Castro ◽  
Neymar Camposeco-Montejo ◽  
Sonia Ramírez-Barrón ◽  
...  

The objective of this study was to determine the oxidative stress and the physiological and antioxidant responses of coriander plants (Coriandrum sativum) grown for 58 days in soil with zinc oxide nanoparticles (ZnO NPs) and zinc sulfate (ZnSO4) at concentrations of 0, 100, 200, 300, and 400 mg of Zn/kg of soil. The results revealed that all Zn compounds increased the total chlorophyll content (CHLt) by at least 45%, compared to the control group; however, with 400 mg/kg of ZnSO4, chlorophyll accumulation decreased by 34.6%. Zn determination by induction-plasma-coupled atomic emission spectrometry (ICP–AES) showed that Zn absorption in roots and shoots occurred in plants exposed to ZnSO4 at all concentrations, which resulted in high levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA). Only at 400 mg/kg of ZnSO4, a 78.6% decrease in the MDA levels was observed. According to the results, the ZnSO4 treatments were more effective than the ZnO NPs to increase the antioxidant activity of catalase (CAT), ascorbate peroxidase (APX), and peroxidases (POD). The results corroborate that phytotoxicity was higher in plants subjected to ZnSO4 compared to treatments with ZnO NPs, which suggests that the toxicity was due to Zn accumulation in the tissues by absorbing dissolved Zn++ ions.


Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4795 ◽  
Author(s):  
Yasmine Abdallah ◽  
Mengju Liu ◽  
Solabomi Olaitan Ogunyemi ◽  
Temoor Ahmed ◽  
Hatem Fouad ◽  
...  

Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most devastating diseases, resulting in significant yield losses in rice. The extensive use of chemical antibacterial agents has led to an increase the environmental toxicity. Nanotechnology products are being developed as a promising alternative to control plant disease with low environmental impact. In the present study, we investigated the antibacterial activity of biosynthesized chitosan nanoparticles (CSNPs) and zinc oxide nanoparticles (ZnONPs) against rice pathogen Xoo. The formation of CSNPs and ZnONPs in the reaction mixture was confirmed by using UV-vis spectroscopy at 300–550 nm. Moreover, CSNPs and ZnONPs with strong antibacterial activity against Xoo were further characterized by scanning and transmission electron microscopy, Fourier-transform infrared spectroscopy, and X-ray diffraction. Compared with the corresponding chitosan and ZnO alone, CSNPs and ZnONPs showed greater inhibition in the growth of Xoo, which may be mainly attributed to the reduction in biofilm formation and swimming, cell membrane damage, reactive oxygen species production, and apoptosis of bacterial cells. Overall, this study revealed that the two biosynthesized nanoparticles, particularly CSNPs, are a promising alternative to control rice bacterial disease.


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